Browsing by Author "Kiziltunc, Ahmet"

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Determination of Endothelial Nitric Oxide Synthase Gene Polymorphism and Plasma Asymmetric Dimethyl Arginine Concentrations in Patients With Lung Cancer
(Aves, 2020) Bayraktutan, Zafer; Kiziltunc, Ahmet; Bakan, Ebubekir; Alp, Hamit Hakan
Objective: Genetic factors can contribute to both the occurrence and development of lung cancer. This study aimed to investigate endothelial nitric oxide synthase (eNOS) G894T and T-786C polymorphisms and plasma asymmetric dimethylarginine (ADMA) levels of lung cancer patients in comparison with healthy subjects. Materials and Methods: A total of 200 subjects, 100 patients with lung cancer and 100 healthy volunteers were included in this study. To determine eNOS gene polymorphisms, we collected and analyzed blood samples with polymerase chain reaction (PCR). Plasma ADMA levels were evaluated by high-performance liquid chromatography (HPLC). Results: The difference in gene polymorphisms between lung cancer patients and healthy controls were insignificant. However, lung cancer patients had statistically significantly higher plasma ADMA levels than healthy controls. The patients and control groups with CC polymorphisms and TT polymorphisms on eNOS T-786C and G894T gene regions had higher plasma ADMA levels. The CC polymorphisms and plasma ADMA levels were higher in patients with small-cell lung cancer compared to those in patients with non-small-cell lung cancer. Conclusion: Although eNOS gene polymorphisms had no significant difference between lung cancer patients and healthy controls, plasma ADMA levels were higher in lung cancer patients compared to healthy controls. Our study suggests that CC genotypes and elevated plasma ADMA levels might be associated with small-cell lung cancer.
Effects of Composite Restorations on Oxidative Stress in Saliva: an in Vivo Study
(Elsevier Taiwan, 2015) Gul, Pinar; Akgul, Nilgun; Alp, Hamit Hakan; Kiziltunc, Ahmet
Background/purpose: This study is aimed at detecting and quantifying monomers from dental composite filling materials and investigating effects of the released monomers in saliva malondialdehyde (MDA) levels and in two antioxidant enzyme levels after dental composite filling materials were applied to healthy volunteers. Materials and methods: Fifty-two individuals (32 female and 20 male) participated in the research study. Saliva samples were collected in scheduled time intervals and MDA, superoxide dismutase, and glutathione peroxidase levels were measured. In addition, saliva samples were collected in order to quantify triethyleneglycol dimethacrylate, hydroxyethyl methacrylate, bisphenol-A diglycidyl methacrylate, and urethane dimethacrylate using high-performance liquid chromatography. Statistical analysis of obtained data was performed by repeated measures variance analysis. A P value <0.05 was considered as statistically significant. Results: As a result of the statistical analysis, the MDA and glutathione peroxidase levels measured at all time intervals were significantly higher when compared to the baseline levels (P < 0.05). Superoxide dismutase values measured after 7 days showed a statistically significant decrease (P < 0.001). The residual monomer levels showed a significant increase within 7 days, and the maximum amount of release was observed generally in the 7-day period, and the release in monomers except for bisphenol-A diglycidyl methacrylate showed a significant decrease in 30 days (P < 0.05). Conclusion: Because of monomer release after the restoration process and also differences in antioxidant enzyme activity, composite materials may lead to oxidative stress. Copyright (C) 2014, Association for Dental Sciences of the Republic of China. Published by Else-vier Taiwan LLC. All rights reserved.
The Effects of Overhang Amalgam Restorations on Levels of Cytokines, Gingival Crevicular Fluid Volume and Some Periodontal Parameters
(Mosher & Linder, inc, 2016) Ilday, Nurcan Ozakar; Celik, Neslihan; Dilsiz, Alparslan; Alp, Hamit Hakan; Aydin, Tuba; Seven, Nilgun; Kiziltunc, Ahmet
Purpose: To evaluate clinical periodontal findings and GCF levels of tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6) and interleukin 8 (IL-8) in patients with overhang amalgam restorations before and after overhang restoration removal. Methods: 22 volunteer subjects (age range: 22-42 years old) with 22 overhang Class II amalgam restorations were selected. GCF samples were obtained from adjacent and contralateral teeth for IL-6, IL-8 and TNF-alpha, measurements and analyzed using enzyme-linked immunosorbent assay (ELISA) kits. The amalgam overhangs were removed and the readings were repeated at the end of 1 week. Clinical periodontal assessments, including gingival index (GI) and plaque index (PI) were performed at baseline and after 1 week. Data were analyzed using the paired t test and independent sample t-test at a significance level of 0.05. Correlations were investigated using Pearson correlation analysis. Results: A significant reduction in GI and PI was observed after removal of the overhanging restoration on Day 7. (P< 0.05) However, there was no significant difference between IL-6 (P= 0.857), IL-8 (P= 0.579) and TNF-alpha (P= 0.958) levels before and after overhang restoration removal. There were no significant correlations between laboratory findings and clinical parameters.
The Effects of Silorane Composites on Levels of Cytokines and Periodontal Parameters
(Medknow Publications & Media Pvt Ltd, 2013) Ilday, Nurcan Ozakar; Celik, Neslihan; Dilsiz, Alparslan; Alp, Hamit Hakan; Aydin, Tuba; Seven, Nilgun; Kiziltunc, Ahmet
Aims: The purpose of this pilot study was to determine the effects of silorane composites on gingival crevicular fluid (GCF) levels of tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6) and IL-8, GCF volume and clinical periodontal parameters in patients with silorane composite restorations before and after restorative treatment. Materials and Methods: A total of 20 systemically healthy non-smokers, 12 female and 8 male (age range: 24-46 years), presenting with 25 instances of primary dentine caries with subgingival margins were selected for this study. Approval was obtained from the university ethics committee and treatment plans were approved by the patients. GCF samples were obtained with periopaper strips from relevant teeth for IL-6, IL-8 and TNF-alpha measurements. Each sample was stored at 80 degrees C and analyzed using the enzyme-linked immunosorbent assay (ELISA) kits. Cavities were prepared according to the common principles for adhesive restorations and restored with a silorane adhesive system (Silorane System Adhesive (3M ESPE) and silorane composite (Filtek Silorane, 3M ESPE). Cytokine levels were reassessed 2 weeks after restorative treatment. Data were analyzed using the independent t-test at a significance level of a =0.05. Associations between parameters were analyzed using Pearson correlation analysis. Results: A significant increase in gingival index (GI) and plaque index (PI) were observed after 15 days (P < 0.05). GCF volume, IL-6, IL-8 and TNF-alpha levels exhibited significant differences before and after restorative treatment (P < 0.05). There were strong positive correlations among parameters except for PI/GCF volume and GI/GCF volume. Conclusion: Within the limitations of this investigation, silorane composites may have some negative effects on cytokine levels, clinical parameters and GCF volume.