Browsing by Author "Koşal, V."

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Adverse Effects of Oxytetracycline and Enrofloxacin on the Fertility of Saanen Bucks
(Springer Science and Business Media B.V., 2021) Yücel, U.M.; Koşal, V.; Kara, M.; Taşpınar, F.; Uslu, B.A.
This study aimed to determine the adverse effects of oxytetracycline and enrofloxacin application on the fertility of Saanen bucks. For this purpose, twenty-four bucks were divided into three groups. Group I (control group) received only 5 ml of 0.9% NaCl for 7 days, group II was given a single dose of 20 mg/kg oxytetracycline and group III was given at a dose of 2.5 mg/kg per day for 7 days intramuscularly. Serum and semen samples were collected from the bucks at post-treatment 1, 3, 5, 7, and 9 days and examined spermatological parameters (quantity, motility, density, abnormal sperm ratio, and live-dead sperm ratio), serum testosterone levels (with ELISA) and sperm DNA parameters (with Comet assay). The results showed no change in sperm volume, abnormal sperm rate, and dead-live sperm ratio in group II and III following oxytetracycline and enrofloxacin administration. However, a decrease in sperm density, sperm motility, mass activity, and testosterone levels, and an increase in sperm DNA damage were detected. These spermatological parameters (density, motility, mass activity) and testosterone levels were less decreased and sperm DNA damage was less increased in group II than group III. The greater damage in group III may be attributed to the longer duration of enrofloxacin administration compared to oxytetracycline and the effect of enrofloxacin on DNA. The results obtained from this study suggest that usage of oxytetracycline and especially enrofloxacin should be restricted and antibiotics with fewer side effects on sperm should be preferred in Saanen bucks during the reproduction period. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
Changes in Sperm Parameters Following Administration of Theophylline, a Competitive Antagonist of Adenosine in Rats Exposed To Bleomycin, a Chemotherapeutic Agent
(Ataturk Universitesi, 2019) Belhan, S.; Oto, G.; Arihan, O.; Koşal, V.
The aim of the present study is to determine the effects of theophylline (THP), a methylxanthine derivative, on the sperm parameters of rats exposed to bleomycin (BLM) as a chemotherapeutic agent. The study was performed on 32 male rats. The rats in Group 1 were administered only with sterile saline intratracheally. The rats in Group 2 were administered a single dose of 7.5 mg/kg of BLM intratracheally. The rats in Group 3 received a single dose of 7.5 mg/kg of BLM intratracheally. In addition, THP was administered to rats in Group 3 intraperitoneally at a dose of 75 mg/kg/ day for 14 days. The rats in group 4 were administered an intraperitoneal injection of THP at a dose of 75 mg/kg/day for 14 days. Motility, sperm density, abnormal sperm and dead sperm rates were examined. Significant deteriorations were observed in sperm parameters of rats that were administered BLM. In sperm parameters of rats that were administered THP, it was determined that it only increased motility, but did not significantly change sperm density, abnormal sperm and dead sperm count. In this study, THP only increased motility. There was no positive effect on sperm parameters except motility. © 2019 Ataturk Universitesi. All rights reserved.
The Effect of Tarantula Cubensis (Theranekron) on Some Reproductive Parameters in Male Rats
(University of Tehran, Faculty of Veterinary Medicine, 2023) Koşal, V.; Keleş, Ö.F.
Background: Tarantula cubensis (theranekron), obtained from the poison of Cuban Tarantula, is a homeopathic drug frequently used in animal health. Objectives: The effect of theranekron on reproductive parameters in male animals is unknown. In this study, the effect of theranekron on male reproductive parameters was investigated. Methods: Three different groups of rats were created: control (n=8) (no application), theranekron I (n=8) (subcuteneous injection of 0.3 mg/kg on days 0, 5, and 10), and theranekron II (n=8) (subcuteneous injection of 0.3 mg/kg on days 0, 5, and 10). Control and theranekron I groups were sacrificed at the end of the 4 weeks, and the theranekron II group was sacrificed at the end of the 6 weeks. Sperm motility, density, abnormal rate, DNA damage, and testicular histopathology were examined. Results: No statistical difference was observed in the parameters examined between the study groups (P>0.05). It was determined that the theranekron did not change sperm motility, density and spermatozoa ratio, also no histopathological changes, and sperm DNA damage. Conclusion: There is no harm in using theranekron in male animals during the breeding and nonbreeding seasons. Copyright © 2023 by Prusa Medical Publishing.
The Effects of Levamisole and Albendazole on Spermatological Parameters, Testosterone Levels, and Sperm Dna Damage in Saanen Bucks
(Springer Science and Business Media B.V., 2021) Yücel, U.M.; Koşal, V.; Taşpınar, F.; Uslu, B.A.
This study aimed to investigate the effect of levamisole and albendazole on spermatological parameters, testosterone levels, and sperm DNA damage in Saanen bucks. For this purpose, twenty-four Saanen bucks were divided into three groups as control, levamisole, and albendazole administration group. The control group received only water (20 ml, oral), the levamisole group received 7.5 mg/kg of levamisole (2 oral tablets once daily for 2 days) + water (20 ml, oral), and the albendazole group received 7.5 mg/kg of albendazole (1 oral tablet) + water (20 ml, oral). Semen and blood samples were collected from all animals, both before drug application (day 0) and within a 2-day interval after drug application between day 1 (day of the treatment) and day 11. Spermatological parameters were evaluated immediately after collection. Testosterone levels were also measured from the blood samples with ELISA. Sperm DNA damage was determined with comet assay. The present research showed that especially albendazole administration decreased spermatological parameters and levamisole administration decreased testosterone levels. Significant sperm DNA damage was seen after both albendazole and levamisole administration. As a result, albendazole and levamisole administration should be used carefully on Saanen bucks, especially during the breeding season. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
Protective Effect of Subsieve Grape Extract (Vitis Vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats
(Ataturk Universitesi, 2023) Koşal, V.; Avci, A.; Bingöl, N.T.; Keleş, Ö.F.; Kömüroğlu, A.U.; Okulmuş, Ç.
Heat stress (HS) has negative effects on reproductive parameters in male animals. This study was designed to investigate the protective effects of Vitis vinifera L., which has a natural antioxi-dant property, on reproductive parameters, testicular oxidative stress, reproductive hormones, and testicular histopathology in male rats treated with HS. Fifty male rats were divided into 5 different groups (control, HS, HS + 100, HS + 200, and HS + 300). It was determined that subsieve V. vinifera extract given at different doses eliminated the negative effects of HS administration on reproductive parameters. It was determined that V. vinifera increased sperm motility and density and decreased the rate of abnormal sperm regardless of dose (P <.001). It was determined that increased oxidative stress and decreased luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels as a result of HS administration improved in the extract-treated groups (P <.05), and FSH and testosterone levels increased in the HS + 200 and HS + 300 groups compared to the control group (P <.001). Histopathologically, it was determined that the degen-eration, edema, and congestion caused by HS disappeared due to the extract administration. As a result of this study, the protective effect of subsieve V. vinifera extract on HS on male reproductive parameters was revealed. © Author(s).