Browsing by Author "Koc, A"

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Effects of a Gnrh Agonist on Oocyte Number and Maturation in Mice Superovulated With Ecg and Hcg
(Elsevier Science inc, 2004) Kanter, M; Yildiz, C; Meral, I; Koc, A; Tasal, I
The objective was to investigate the effects of a gonadotropin-releasing hormone agonist (GnRH) on ovulation rate and the number and maturation of oocytes in mice superovulated with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Thirty 3-month-old BALB/C female mice (weight: 25-30 g) were assigned to three experimental groups: control, superovulated, and superovulated with GnRH pretreatment (n = 10 per group). Control mice received an i.p. injection of 0.1 ml physiological saline solution. Superovulation was induced with 5 IU eCG (i.p.) and 5 IU hCG 48 It later. Mice in the superovulated with GnRH pretreatment group were given GnRH (20 mg/kg Fertirelin, i.m.), 24 h before superovulation. Thirteen hours after hCG administration, mice were sacrificed by cervical dislocation and blood samples were collected to determine serum progesterone concentration (by radioimmunoassay). Ovaries and oviducts were also harvested to enumerate corpora lutea and cumulus-enclosed oocytes. Progesterone concentrations were not significantly different among groups. The oocyte number and the maturation, ovulation rate, and the number of corpora lutea were higher in GnRH-treated mice than both controls and superovulated mice. In conclusion, GnRH given 24 h before superovulation with eCG-hCG increased the number and maturation of oocytes and the rate of ovulation in mice. (C) 2003 Elsevier Inc. All rights reserved.
Effects of Cadmium Exposure on Morphological Aspects of Pancreas, Weights of Fetus and Placenta in Streptozotocin-Induced Diabetic Pregnant Rats
(Humana Press inc, 2003) Kanter, M; Yoruk, M; Koc, A; Meral, I; Karaca, T
This study was designed to evaluate the effects of Cd exposure on morphological aspects of beta-cell and weights of fetus and placenta in streptozotocin (STZ)-induced diabetic pregnant rats. Ninety-nine virgin female Wistar rats (200-220 g) were mated with 33 males for at least 12 h. From the onset of pregnancy, the rats were divided into four experimental groups (control, Cd treated, STZ treated, and Cd+STZ treated). The Cd-treated group was injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl, starting at the onset of pregnancy throughout the experiment. Diabetes was induced on the 13th d of pregnancy by a single intraperitoneal injection of STZ in STZ-treated group. In addition to the daily injection of Cd, a single intraperitoneal injection of STZ was also given on the 13th d of pregnancy in the Cd+STZ-treated group. The rats received the last injection 24 h before being sacrificed and 10 randomly selected rats in each group were sacrificed on the 15th and 20th d of pregnancy. Blood samples were taken for the determination of the serum glucose and insulin levels. Maternal pancreases, fetuses, and placentas of sacrificed rats in all groups were harvested (fetal pancreas was also harvested only on the 20th d of pregnancy) for morphological and immunohistochemical examinations. Cd exposure alone caused a degeneration, necrosis, and weak degranulation, but Cd exposure with STZ caused a severe degeneration, necrosis, and degranulation in the beta-cells of the pancreatic islets. No morphological or immunohistochemical differences were found in beta-cells of fetal pancreatic islets of control or other treatment groups. Cd exposure alone also decreased the fetal and placental weights. The administration of STZ alone, on the other hand, increased the placental weight. Cd, STZ, and Cd+STZ administration increased the glucose and decreased the insulin level. The increase in glucose and decrease in insulin levels were higher when Cd and STZ were given together. All of these changes were more severe on the 20th d than those on the 15th d of the pregnancy. It is concluded that Cd exposure during pregnancy may reduce the birth and placental weights and produce necrosis, degeneration, and degranulation in beta-cells of pancreatic islets, causing an increase in the serum glucose level. These changes might be severe in diabetic pregnant mothers.
Morphological Quantitative Changes in the Number of Lymphocytes, Macrophages and Plasma Cells in the Uterus and Lymph Nodes of Rats Exposed To the Systemic Administration of Bcg
(Tohoku Univ Medical Press, 2003) Kanter, M; Gul, A; Meral, I; Koc, A; Ilhan, M; Erdogan, E
KANTER, M., GUL, A., MERAL, I., Koc, A., ILHAN, M. and ERDOGAN, E. Morphological Quantitative Changes in the Number of Lymphocytes, Macrophages and Plasma Cells in the Uterus and Lymph Nodes of Rats Exposed to the Systemic Administration of BCG. Tohoku J. Exp. Med., 2003, 199 (4), 219-228 - This study was designed to investigate the effect of systemic administration of BCG on the morphological quantitative changes in the number of lymphocytes, macrophages and plasma cells in the uterus and lymph nodes of rats. Thirty female virgin Wistar Albino rats, aging 6 months and weighing between 200-250 g, were assigned to the two experimental groups; BCG treated and controls (n = 15). BCG group received 0.1 ml BCG in tail skin and control group received 0.1 ml saline at the same place. Two weeks after injections, rats in both groups were anesthesized with a high dose of ether and decapitated. Uterus and ileocecal lymph nodes were processed to determine alpha napthyl acid esterase (ANAE)-positive T lymphocytes and macrophages. The plasma cells were stained with the methyl green-pyronin method. It was found that the numbers of T lymphocytes, macrophages and plasma cells in the uterus and the ileocecal lymph nodes of BCG treated group significantly increased indicating the presence of an immune response to the systemic BCG administration. It was concluded that the systemic administration of BCG increases humoral and cellular immunity in endometrium, myometrium and regional lymph nodes. The immune deficiency system plays an important role in the pathogenesis of endometriosis. Therefore, the endometriosis might be prevented by using periodical administration of BCG. However, further experimental and clinical studies associated with these issue are required.
A Rat Model for the Immune Response To the Intrauterine Administration of Bcg
(Scandinavian Federation Laboratory Animal Science, 2002) Kanter, M; Yoruk, M; Koc, A; Meral, I; Timurkan, HH
This study as designed to investigate the changes in the numbers of lymphocytes. macrophages and plasma cells in the uterus and ileocecal lymph nodes of rats exposed to the intrauterine administration of Bacillus-Calmette Guerin (BCG). Thirty female Wistar Albino rats. age 6 months and weighing between 200-250 g. ere assigned to the two experimental groups BCG treated and controls (n = 15). The intrauterine BCG injections where made using laparatomy in the diestrous cycle under Rompun and Ketalar anesthesia. 0.1 ml BCG ere injected for each into cornu uteri awhile the control group received 0.1 ml sterile saline in the same place, Two weeks later. the rats in both groups were anesthetized with ether and decapitated. Uterus and ileocecal lymph nodes were processed to determine alpha naphthyl acid esterase (ANAE) - positive T lymphocytes and macrophages. The plasma cells were stained with the methyl green-pyronin method. It was found that the numbers of lymphocytes. macrophages; and plasma cells on the uterus increased (P<0.01) in BCG treated rats. In addition. the number of these cells also increased in the ileocecal lymph nodes indicating the presence of an immune response to the intrauterine BCG administration. It is concluded that although the rat was chosen as a model and BCG as given by the process of laparatomy in this stud,,. intracervical administration of BCG in the uterus should be studied clinically in cases of immune deficiency disorders related to the uterus. such as endometritis. myometritis. pyometra. endometriosis. infertility and implantation problems of domestic animals. to see if there is an increase in the immune response.