Browsing by Author "Kocak, Y."

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Eco-Friendly Production of Platinum Nanoparticles: Physicochemical Properties, Evaluation of Biological and Catalytic Activities
(Springer, 2024) Kocak, Y.; Aygun, A.; Altuner, E. E.; Ozdemir, S.; Gonca, S.; Berikten, D.; Sen, F.
In this research, Vitis vinifera extract was used as a reducing molecule for the biogenic synthesis of platinum nanoparticles (Vv-Pt NPs). Vv-Pt NPs were extensively characterized by various methods. It has been determined that Pt NPs were spherical shaped and 1.513 +/- 0.35 nm in size with TEM analysis. The biological activity of synthesized Pt NPs using Vitis vinifera extract was investigated such as antimicrobial, antioxidant, and DNA cleavage studies. Pt NPs exhibited the highest 2,2-Diphenyl-1-picrylhydrazil (DPPH) radical scavenging activity at 200 mg/L. The synthesized Pt NPs were observed to have significant chemical nuclease activity. Pt NPs were found to have significant antibacterial activity against Gram (-) and Gram (+) bacteria, and the highest antimicrobial activity was observed against E. coli. Then, hydrogen production was investigated due to the catalytic effects of Vv-Pt NPs on the hydrolysis of sodium borohydride (NaBH4). Turnover of frequency (TOF), enthalpy (triangle H), entropy (triangle S), and activation energy (Ea) values were determined as 1217.20 h(-1), 37.70 kJ/mol, -153.66 kJ/mol K, and 40.24 kJ/mol, respectively. It has been observed that synthesized Vv-Pt NPs will be used as nano-drugs and will guide sustainable, renewable, and clean energy studies.
Evaluation of Antioxidant, Antibacterial and Thermal Stability Properties of Silver Nanoparticles Synthesised With Infundibulicybe Gibba Extract
(Springer, 2025) Meydan, I.; Seckin, H.; Kocak, Y.; Okumus, E.; Bekmezci, M.; Sen, F.
Mushrooms have been used by people for centuries for various purposes due to their unique taste, high nutritional content and pharmacological effects. Bioactive components in the structure of mushrooms are an important biological source for the green synthesis of silver nanoparticles (AgNPs) as reducing and stabilizing agents. In this study, AgNPs/Ig were synthesized using the mushroom species Infundibulicybe gibba. The color transformation of the formed nanoparticles from yellow to brown occurred and peaked at 370 nm in the UV-Vis spectrum. The nanoparticles had amorphous shape and their molecular characterization was determined by Fourier transform infrared spectroscopy (FTIR). AgNPs/Ig was much more stable (- 50.02 mV) and its hydrodynamic diameter was much lower (216.50 nm) compared to the mushroom extract. A significant increase in the antioxidant activity (IC50 5.66 mg/ml) and thermal stability of the formed nanoparticles was determined. Lipid peroxidation inhibition of the extract and nanoparticles was measured as IC50 value of 6.75 and 5.51 mg/ml, respectively. In the antimicrobial analysis results, while the mushroom extract did not show any inhibition against the selected microorganisms, AgNPs/Ig showed a low antimicrobial activity. As a result, the synthesis of AgNPs/Ig was carried out through green synthesis, which is environmentally friendly, safe, cost-effective, easy to use and does not contain toxic chemicals, and it has been revealed that AgNPs synthesized using this mushroom species have advantageous potential for use.
Investigation of Mycosynthesized Silver Nanoparticles by the Mushroom Pleurotus Eryngii in Biomedical Applications
(Springer, 2023) Kocak, Y.; Meydan, I.; Karahan, T. Gur; Sen, F.
The mycosynthesis of nanoparticles using mushroom is a promising and innovative approach to Biogenic Sustainable Nanotechnology. In this study, mycosynthesis of silver nanoparticles (AgNPs) was carried out using an edible mushroom extract of Pleurotus eryngii (Pe). AgNPs were characterized by ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) analyses. UV-vis analysis exhibited an absorption peak at 415 nm, which corresponds to the surface plasmon resonance of AgNPs. FT-IR and XRD revealed the presence of bioactive components and face-centered cubic structure in synthesis. SEM and EDX analyze confirmed that the AgNPs were spherical and 10-45 nm in size, and the presence of strong signals from silver metal. The therapeutic properties of AgNPs were tested by DNA (Deoxyribonucleic acid) damage, antioxidant (2,2 '-diphenyl-1-picrylhydrazyl (DPPH) method), and lipid peroxidation (LPO) assays. Biosynthesized AgNPs were found to restore DNA damage at concentrations of 50 and 75 mu g/mL. The highest DPPH radical scavenging activity of AgNPs was recorded as 89.44% at 400 mu g/mL and was more effective than Pe extract. AgNPs demonstrated potent activity in inhibiting LPO with a percentage of 88.38% at a concentration of 300 mu g/mL. The biological activity of AgNPs was found to be concentration dependent and had more activity than the Pe extract. The obtained results predict that the mycosynthesis method is less toxic, cost-effective and environmentally friendly, and the AgNPs produced will contribute to the development of new-generation drugs in the fields of biomedicine and pharmacology.
Role of Trametes Multicolor in Green Nanotechnology Based Antioxidant, Antimicrobial, Lipid Peroxidation Inhibition From Fungi To Nanoparticles
(Springer, 2024) Kocak, Y.; Okumus, E.; Meydan, I.; Seckin, H.; Bekmezci, M.; Sen, F.
Fungi as source for new-bio based materials has a wide range of potential uses in the pharmaceutical, cosmetic, and medical sectors. They contain abundant natural bioactive chemical resources. This study reports the use of Trametes multicolour (Tm) mushroom extract for the ecofriendly production of silver nanoparticles (AgNPs). The color of the mushroom extract turns dark brown after a certain period of time when combined with metal. (AgNPs/Tm) and then showed maximum absorption at 318 nm, the wavelength of the surface plasmon resonance of AgNPs. The morphology of AgNPs/Tm was spherical and an EDX peak in the 3 keV region indicated the presence of Ag atoms. The binding properties of the biocomponents involved in NPs handling and stabilising the NPs were identified using Fourier transform infrared spectroscopy (FTIR) data. The synthesized NPs exhibited much higher stability (- 37.63 mV), antioxidant activity (IC50 18.92 mg/ml) and inhibition against lipid peroxidation (IC50 7.46 mg/ml) compared to the mushroom extract. Although there was a significant decrease in the hydrodynamic diameter of the NPs, there was an increase in their thermal stability and antimicrobial activity. As a result, the existence and current potential of a new biomaterial suitable for cost-effective and large-scale production in the synthesis of AgNPs has been demonstrated for the first time.