Browsing by Author "Korkoca, H"

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The Characterization of Protein Profiles of the Isolated Aeromonas Sobria Strains From Animal Faeces by Sds-Page
(indian veterinary Journal, 2004) Boynukara, B; Korkoca, H; Senler, NG; Gulhan, T; Atalan, E
Aeromonas spp. are members of the Vibrionaceae (Janda, 1991). Although they are common in fresh surface water, their presence in faeces of several animals and humans has been proved (Boynukara et al., 2002; Jindal et al., 1993). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins have been used for typing several bacterial species, including Aeromonads (Stephenson and Tabaqchali 1986; Stephenson et al., 1987). A more sensible approach might be to identify isolates as A. hydrophila, A. sobria or A. caviae, and use an electrophoretic fingerprinting technique to further characterize them (Popoff, 1984), Maclnnes et al. (1979) did the first DNA hybridization experiments with Aeromonas, and concluded that the genus consist of two main evolutionary lines; a diverse group of motile Aeromonads and genetically more homogeneous non-motile Aeromonads. Fanning et al. (1985) confirmed that all strains of Aeromonas were more closely related to each other than to species in other families, and found that motile Aeromonas species could be divided into at least 10 different DNA hybridization. In this study, we aimed to characterize the Aeromonas sobria isolated from Turkish Van cat, chicken, gull and Rainbow trout and identified according to Popoff (1984) using SDS-PAGE method.
Prevalence and Antibiotic Sensitivity of Motile Aeromonas in Dogs
(veterinarni A Farmaceuticka Univerzita Brno, 2003) Ceylan, E; Berktas, M; Korkoca, H; Keles, I; Bozkurt, H; Kurtoglu, MG
The present study was carried out to assess the prevalence of motile Aeromonas sp. in the faeces of healthy dogs and to evaluate its susceptibility to some anti-microbial agents. Rectal swabs from 55 domestic dogs were examined for Aeromonas species using alkaline peptone water (pH 8.4) as the enrichment medium and Aeromonas Selective Agar containing 5 mg/l ampicillin as the plating medium. Identification and antibiotic resistance of motile Aeromonas strains was performed using Gram Negative Breakpoint ID panel. After examining these samples, aeromonads were isolated from three (5.45%) dogs. Of those, one was A. sobria, and two were A. caviae. While all isolated strains were sensitive to amikacin, cefoperazone, cefotaxime, cefotetan, ceftazidime, ceftriaxone, cefuroxime, ciprofloxacin, gentamicin, imipenem, piperacillin, tetracycline, ticarcillin, ticarcillin/clavulanate, tobramycin, and trimethoprim/sulfamethoxazole, they were resistant to ampicillin, and ampicillin/sulbactam. In addition, a strain of A. caviae was also resistant to aztreonam. As a result, in the present study, information about the prevalence of Aeromonas in dogs, susceptibility to antibiotics of these microorganisms and its importance for both animal and public health were discussed.