Browsing by Author "Kose, Yavuz Bulent"

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Assessment of the Enzyme Inhibitory Activities of Salvia Dorystaechas and Its Phytochemical Analysis by Rp-Hplc
(Springer, 2025) Ozupek, Burcin; Pekacar, Sultan; Saltan, Nagehan; Yildiz, Gulsum; Gulcan, Zeynep; Orhan, Didem Deliorman; Kose, Yavuz Bulent
Salvia dorystaechas is an endemic species found around Antalya in Anatolia, and in this study, the antidiabetic, antiobesity, antihyperlipidemic, and antimelanogenic potentials of the extracts (methanol, ethyl acetate, and chloroform) obtained from the root and aerial parts of the plant were evaluated using in vitro enzyme models (alpha-glucosidase, alpha-amylase, pancreatic lipase, cholesterol esterase, and tyrosinase). Root ethylacetate (IC50 6.53 +/- 0.19 mu g/ml) and aerial part chloroform extracts (IC50 24.14 +/- 1.76 mu g/ml) inhibited the pancreatic cholesterol esterase enzyme, as much as, or even more potently than simvastatin (IC50 20.48 +/- 2.70 mu g/ml). All extracts showed weak or no inhibitory activity against other enzymes. RP-HPLC analysis results showed that S. dorystaechas root ethyl acetate extract, which has the strongest pancreatic cholesterol esterase inhibitory activity, can be standardized with rosmarinic acid (3.164 +/- 0.001 mg/g plant) and caffeic acid (1.118 +/- 0.000 mg/g plant). As a result, it can be predicted that this plant, which is also commonly consumed as a tea, can be evaluated as a medicinal tea or medicinal herbal product with further studies due to its strong cholesterol-lowering effect.
Chemical Profile, in Vitro Pharmacological Activity and Satureja Cuneifolia Ten. Evaluation of Essential Oil Based on Distillation Time
(Taylor & Francis Ltd, 2024) Yildiz, Gulsum; Ilgun, Selen; Karatoprak, Gokce Seker; Kose, Yavuz Bulent; Goger, Fatih; Temel, Halide Edip; Demirci, Betul
The medicinal plant Satureja cuneifolia Ten. was widely utilized as spice, tea and traditional medicine. The objective of the current study was to examine the chemical composition and in vitro biological activities (LOX, MMP-1, and MMP-12 enzyme inhibition activity and cytotoxicity on A549 cell line) of Satureja cuneifolia extracts and essential oils. The essential oils of the flowering aerial parts were hydro-distilled at four different distillation times (5, 30, 60, and 180 min) using the Clevenger apparatus. The total essential oil and four fragments were compared in terms of the major component, yield, and distillation time. Volatile compounds of the infusion were extracted by using HS-SPME. Ethanolic extract had the strongest inhibition activity on the LOX enzyme (84.50%), while the essential oils exhibited more cytotoxic activity on the A549 cell line than the extracts. The oils and the infusion were analyzed using GC-MS and the primary chemicals identified by LC-MS/MS.