Browsing by Author "Kurt, Serdal"

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Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows With Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus Aureus
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Kurt, Serdal; Eski, Funda; Mis, Leyla; Demir, Pinar Ayvazoglu
The aim of this study was to investigate effect of Staphylococcus aureus on oxidative stress status (TAS, TOS, OSI), immune system (IL-1 beta, IL-6, TNF-alpha), and mineral (Mg, Fe, Zn, Cu, Na and Ca) concentrations in milk and serum of cows with mastitis. The cows were allocated to three groups according to mammary health status as follows: healthy (Group 1), clinical (Group 2) and subclinical mastitis cows (Group 3). IL-1 beta levels in serum and milk increased in Group 2 compared to Group 1 and Group 3 (P<0.001; P<0.05). Milk IL-6 level was greater in Group 3 and Group 2 than in Group 1 (P<0.01). Blood TNF-alpha (P<0.001), TOS and OSI levels (P<0.01) were higher in Group 2 than other groups. Milk TNF-alpha level increased and blood TAS level decreased in Group 2 compared to Group 1 (P<0.05). Milk TOS (P<0.01) and OSI (P<0.05) levels increased in Group 3 compared to Group 1. Blood and milk Mg levels increased in Group 1 (P<0.05) and Group 2 (P<0.001), respectively. Milk Fe (P<0.01) and Na levels were greater in Group 2 (P<0.001). Blood Zn level was lower in Group 2 compared to Group 3 (P<0.05). While blood Cu level decreased in Group 1 compared to other groups, milk Cu level decreased in Group 3 compared to other groups (P<0.001). In conclusion, S. aureus had significant effects on oxidative stress, cytokine and mineral levels in milk and blood serum of cows with clinical and subclinical mastitis. However, since there were specific changes only in milk OSI and IL-6 levels according to other parameters, it is thought that milk IL-6 and OSI levels may be a diagnostic tool in the detection of subclinical mastitis.
Investigation of the Usability of Kisspeptin and Oxidative Stress Parameters in the Early Diagnosis of Asymptomatic Cystic Endometrial Hyperplasia in Dogs
(Wiley, 2021) Kurt, Serdal; Eski, Funda; Mis, Leyla
This study aimed to investigate the differences in oxidative stress index (OSI) and kisspeptin levels in clinically asymptomatic dogs with cystic endometrial hyperplasia (CEH) compared to healthy and pregnant dogs, and to determine the usability of the obtained results in the diagnosis of asymptomatic CEH. The study comprised three groups; a healthy (n = 8), a pregnant (n = 10) and a CEH (n = 10). All dogs in the three groups were included in the study at the 30 +/- 3th day after estrus, and blood samples were collected for analysis of kisspeptin, total antioxidant status (TAS), total oxidant status (TOS), progesterone (P4), estradiol (E2) and some biochemical parameters (TSH; thyroid stimulating hormone, ALT; alanine aminotransferase, AST; aspartate aminotransferase, ALP; alkaline phosphatase, LDH; lactate dehydrogenase, CRE; creatine and BUN; blood urea nitrogen). In addition, OSI value was calculated. P4 and ALT and BUN levels were significantly lower and higher in CEH group than the pregnant group, respectively (p < .05). While kisspeptin and TAS levels were significantly lower in CEH group compared to the healthy and pregnant groups (p < .01), OSI level increased dramatically. In conclusion, it was confirmed that CEH clearly affected kisspeptin and OSI levels, and it is thought that these parameters may be an alternative diagnostic tool for the detection of CEH after further studies.
Monitoring of Oxidative Stress and Tnf-Α Status During the Healing Process in Hair Goats With Metritis
(2022) Eski, Funda; Kurt, Serdal; Mis, Leyla
Objective: This study aimed to monitor oxidative stress parameters including total antioxidant status\r(TAS) and total oxidant status (TOS), and tumor necrosis factor-alpha (TNF-α) level during the healing\rprocess in hair goats with metritis.\rMaterials and Methods: This study was carried out on a total of 25 hair goats with metritis (n=10; Group\r1) and healthy (n=15; Group 2). The beginning of the study was accepted as day 0. In both Group 1 and\rGroup 2, blood samples were collected on days 0 (first measurement day), 14 (second measurement day)\rand 28 (third measurement day) of the study for TAS, TOS and TNF-α analyzes. In addition, oxidative\rstress index (OSI) was calculated using TAS and TOS values.\rResults: It was found that TAS level decreased in Group 1 compared to Group 2 at the first measurement\rday (p<0.05). However, TOS, OSI and TNF-α levels increased in Group 1 compared to Group 2 (p<0.01).\rAt the second measurement day, TOS and OSI values were higher in Group 1 than Group 2 (p<0.05).\rConclusion: In conclusion, the antioxidant system weakened, oxidative stress and TNF-α levels increased\rin animals with metritis compared to healthy animals. However, at the third measurement time, all\rparameters became similar between groups.
Tiftik Keçilerinde Doğum Şeklinin Bazı Oksidatif Stres Belirteçleri ve Hormon Konsantrasyonları Üzerine Etkisi
(2021) Eski, Funda; Kurt, Serdal; Çetin, Nebi
Güç doğum, spontan doğumun belirli bir zaman içerisinde herhangi bir müdahale olmadan gerçekleşemediği olguolarak tanımlanmaktadır. Güç doğum steroid hormon seviyesi ile ilişkili olabilmekte ve oksidatif stres üzerine etkiedebilmektedir. Bu çalışmanın amacı, normal ve sezaryen ile doğum yapan Tiftik keçilerinde malondialdehid (MDA),süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), total glutatyonun (GSH) progesteron (P4) ve östradiol (E2)konsantrasyonlarını değerlendirmektir. Yapılan çalışmada, keçiler normal (n=10) ve sezaryen ile doğum (n=10) olmak üzere2 gruba ayrıldı. Her iki grubun kan örnekleri, doğumdan sonraki ilk yarım saat içinde jugular venadan alındı. MDA, GSH-Px,total GSH seviyelerinde gruplar arasında önemli bir farklılık (P>0.05) görülmedi. Sezaryen grubunda hem SOD (P <0.01), hemde P4 ve E2 konsantrasyonları anlamlı olarak daha yüksekti (P <0.05). Sonuç olarak, sezaryen ile doğum yapan keçilerdeSOD, P4 ve E2 seviyelerinde farklılıklar gözlemlendi. P4 ve E2 konsantrasyonlarındaki farklılıkların güç doğumunetiyopatogenezinde rol alabileceği ve güç doğuma bağlı artan strese karşı mücadele için endojen bir antioksidan madde olanSOD’un üretiminin arttığı kanaatine varıldı.