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Browsing by Author "Kurt, Z."

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    Article
    New Insights Into Tomato Spotted Wilt Orthotospovirus (Tswv) Infections in Türkiye: Molecular Detection, Phylogenetic Analysis, and in Silico Docking Study
    (Academic Press, 2023) Usta, M.; Guller, A.; Demİrel, S.; Korkmaz, G.; Kurt, Z.
    Seventy-eight tomato and pepper plants showing symptoms were tested for tomato spotted wilt orthotospovirus (TSWV) using specific primers targeting the full coat protein gene (CP) through RT-PCR. Plant samples were collected in Denizli region, Türkiye during September 2021. The PCR screening results revealed that 23 samples were infected with TSWV, indicating an infection rate of 29.48%. PCR products were subsequently sequenced bidirectionally, and the obtained sequences were deposited in GenBank under the accession numbers ON323583-84 for pepper, and OQ597214 for tomato. Phylogenetic analysis demonstrated a close relationship between the Turkish-Denizli TSWV isolates and previously reported isolates from tomato and pepper plants in Türkiye (KM407603), Hungary (KJ649612), and Serbia (GU369723). Moreover, in this study, three commercial chemicals and five selected phytochemicals were docked against the TSWV CP to assess their binding energies. The docking results indicate that the tested phytochemicals exhibit promising performance compared to other commercial chemicals. This study represents the first molecular and phylogenetic report on TSWV isolates in pepper and tomato plants in Denizli province, Türkiye. Furthermore, the interaction between TSWV and the selected compounds through in silico docking has been reported for the first time. © Articles by the authors; Licensee UASVM and SHST, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
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    Article
    Presence of 'candidatus Phytoplasma Trifolii' in Pepper Plants Showing Yellowing and Bushy Appearance From Iǧdir Province of Turkey
    (Ege Universitesi, 2022) Güller, A.; Usta, M.; Korkmaz, G.; Demirel, S.; Kurt, Z.
    Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms. Material and Methods: In this study, plants collected from Iǧdir province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used. Results: In the tests performed, approximately 1.2 kb of DNA fragments specific to phytoplasma were obtained. The 16S rRNA nucleotide sequence (1254 bp in length) (OM663745) revealed that it was showed more than 99.44% nucleotide similarity to other 'Ca. P. trifolii' members. The tentative RFLP and phylogenetic analyzes performed proved the 'Ca. P. trifolii' the infection from the Clover proliferation group (16SrVI) group and subgroup A in symptomatic pepper plants. Conclusion: The presence of 'Ca. P. trifolii' in naturally infected peppers in Iǧdir province of Turkey was detected using PCR-RFLP and cladistic analysis. © 2021 Authors.