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Browsing by Author "Kurt, Zeynelabidin"

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    Coat Protein of Alfalfa Mosaic Alfamovirus (Amv) From Türkiye: Genetic Inference and in Silico Docking Analysis for Potential Antiphytoviral Purposes
    (Univ Agr Sci & veterinary Med Cluj-napoca, 2024) Demirel, Serap; Guller, Abdullah; Usta, Mustafa; Kurt, Zeynelabidin; Korkmaz, Gulustan
    In 2021, a study was conducted in the Denizli region of Turkiye to investigate the phylogenetic relationship and presence of alfalfa mosaic alfamovirus (AMV) infecting pepper plants exhibiting viral disease symptoms. A total of 57 samples were collected, of which twenty-four tested positive for AMV with reverse transcriptase polymerase chain reaction (RT-PCR) assays. Samples from pepper plants displaying virus symptoms gave positive bands on the agarose gel, while healthy plants yielded negative results. One of the positive samples was randomly selected, cloned and sequenced. This sequence of the Denizli AMV isolate (753 bp) was recorded in the GenBank database with accession number OQ845956. The nucleotide sequence showed a high nucleotide consensus of 97%-99% compared with the nucleotide sequences of the same variants from different origins in GenBank. According to the phylogenetic tree generated through the Neighbour Joining (NJ) method, this AMV isolate belongs to the same group as Iranian isolates from various of hosts. Furthermore, in silico docking analysis of the coat protein (CP) of the AMV isolate and promising 12 essential oil compounds was performed to enable potential antiviral drug development. Docking study showed that eucalyptol, eugenol and carvacrol can make important contributions to the advancement of drug -based strategies for the managing of plant viruses by interacting with the virus coat protein of high binding energies, -5.3, -5.2 and -5.0 kcal mol-1, respectively. Although the presence of AMV in Denizli province has been reported previously, this study reports the phylogenetic relationships and docking analysis of the new AMV isolate in pepper crops.
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    Recombinant Tritin Protein Exhibits Antiviral Activity Against Zucchini Yellow Mosaic Virus
    (BMC, 2025) Demirel, Serap; Usta, Mustafa; Korkmaz, Gulustan; Kurt, Zeynelabidin
    BackgroundRibosome-inactivating proteins (RIPs) are a group of proteins known to inhibit protein synthesis and contribute to plant defense responses. Although the antiviral properties of various RIPs have been demonstrated, the antiviral potential of tritin, a type 1 RIP from bread wheat (Triticum aestivum L.), remains unexplored. This study aimed to investigate the antiviral activity of recombinant tritin against zucchini yellow mosaic virus (ZYMV) in zucchini (Cucurbita pepo L.) plants.MethodsThe tritin gene was isolated from the wheat cultivar Kutluk-94, cloned into the pETDuet-1 expression vector, and expressed in Escherichia coli BL21 (DE3) cells. Following induction, recombinant tritin was purified using Ni-NTA affinity chromatography. Antiviral activity was assessed by measuring morphological parameters, disease severity, and the expression levels of the ZYMV Coat Protein (CP) gene and the Pathogenesis-Related 1 (PR1) through quantitative real-time PCR.ResultsTritin-treated plants exhibited significantly lower ZYMV-CP gene expression compared to virus-inoculated controls at 3- and 15- days post-inoculation. Furthermore, PR1 gene expression was upregulated in response to tritin application, suggesting the activation of systemic defense pathways. Morphological assessments revealed dose-dependent phytotoxic effects, including reductions in chlorophyll content and plant growth at higher tritin concentrations.ConclusionThis study represents the first report in demonstrating that recombinant tritin exhibits antiviral activity against ZYMV, reducing viral replication and enhancing defense gene expression in zucchini plants. However, the phytotoxic effects observed at higher concentrations suggest the need for dose optimization before agricultural application. These findings provide a promising basis for the development of RIP-based antiviral strategies to improve crop tolerance.
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    Türkiye’nin Bingöl İli Bal Arı Kolonilerinde Deforme Kanat Virüsü ve Kronik Arı Felci Virüsü’nün Prevalansı
    (2023) Güller, Abdullah; Balkaya, Adnan; Kurt, Zeynelabidin; Usta, Mustafa
    Bal arılarını infekteleyen virüsler dünyanın her yerinde bal arılarının her gelişim evresinde infeksiyona neden olan ve ekonomik zarara yol açan hastalıklardır. Bu araştırmada dünya çapında yaygın olan iki bal arısı virüsünün (deforme kanat virüsü [DWV] ve kronik arı paraliz virüsü [CBPV]) varlığı ve yaygınlığını belirlemek için bir survey çalışması gerçekleştirilmiştir. Viral RNA’yı belirlemek amacıyla Bingöl ili sınırlarındaki 9 farklı lokaliteden toplam 128 arılık ziyaret edilmiştir. Toplanan 384 bal arısı örneği her virüse özgü genom spesifik primerler kullanılarak moleküler olarak (revers-transkriptaz polimeraz zincir reaksiyonu, RT-PCR) testlenmiştir. 128 arılığın 28’i (%21.87) DWV için pozitif reaksiyon vermiştir fakat hiçbir arılıkta CBPV patojeni tespit edilmemiştir. Pozitif örneklerden rastgele seçilen birinin klonlanarak ortaya çıkarılan 702 bazlık nükleotit dizisi gen bankasına MZ357973 ulaşım numarasıyla kaydedilmiştir. NCBI veri tabanındaki BLASTn analizine göre, Bingöl ilinde belirlenen DWV etmeninin nükleotit dizisi aynı virüsün diğer izolatlarıyla kıyaslandığında %77.82-98.45 arasında değişen nükleotit benzerliği göstermiştir. Ayrıca, farklı orijinlerden izolatların kullanıldığı filogenetik ağaca dayanarak bizim izolatımız Türkiye’den ErzincanDWV izolatıyla yakın filogenetik ilişki içerisinde olduğu tespit edilmiştir. Literatür taramalarına göre, bu çalışma Bingöl ilinde DWV ve CBPV yaygınlığını ve varlığını DNA temelli yöntemlerle ortaya koyan ilk kayıt niteliğindedir