Browsing by Author "Levent, A."

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The Changes in the Serum, Liver, Kidney Protein and Genomic Dna Profiles in Rats Treated With 7,12-Dimethylbenz(a) Anthracene and Plantago Major L
(2014) Berber, I.; Ekin, S.; Levent, A.; Oto, G.
In the study, the changes in the serum, liver, kidney protein and genomic DNA profiles in Wistar albino rats administrated to 7,12-dimethylbenz(a)anthracene (DMBA) and Plantagomajor L. were investigated. SDS-PAGE profiling of the serum proteins showed that the levels of two proteins molecular weighing 140.8 kDa and 46.8 kDa were significantly lower on the 0th day of treatment than on the 60th day of application in the groups. Furthermore, two proteins (34.5 and 22.3 kDa) were solely present in DMBA-treated liver cell lysates. On the contrary, the kidney protein profiles did not show significant banding variations in groups. Genomic DNA analysis also confirmed that DNA extracted from liver and kidney cells did not fragment in any of the groups after the 60th study day. The proteins 34.5 and 22.3 kDa in liver cell lysates at the 60th day in DMBA-treated group could be extra-prognostic indicators for severe DMBA toxicity.
Trace Heavy Metal Contents and Mineral of Rosa Canina L. Fruits From Van Region of Eastern Anatolia, Turkey
(2010) Levent, A.; Alp, S.; Ekin, S.; Karagöz, S.
Trace heavy metal and mineral levels in four different sites of Rosa canina L. fruits from the Van region of Eastern Anatolia, Turkey were determined by atomic absorption spectrometry. The trace heavy metal contents and mineral of Rosa canina L. fruit samples were found in the ranges: 6.01-9.29 ppm) for copper, 0.0063-0.0248 ppm for cadmium, 0.111- 0.273 ppm for lead, 0.0328- 0.0830 ppm for cobalt, 35.29-66.7 ppm for manganese, 21.94-30.87 ppm for iron, 7.98-13.32 ppm for zinc, 294.08- 392.33 ppm for magnesium, 1395.88-1792.70 ppm for calcium, 1323.12- 1358.27 ppm for sodium and 2106.48-2445.17 ppm for potassium. Results obtained are in agreement with data reported in the literature.
Voltammetric Studies on the Potent Carcinogen, 7,12-Dimethylbenz[a]anthracene: Adsorptive Stripping Voltammetric Determination in Bulk Aqueous Forms and Human Urine Samples and Detection of Dna Interaction on Pencil Graphite Electrode
(Elsevier, 2010) Yardim, Y.; Keskin, E.; Levent, A.; Ozsoz, M.; Senturk, Z.
7,12-Dimethylbenz[a]anthracene (DMBA), is a widely studied polycyclic aromatic hydrocarbon that has long been recognized as a very potent carcinogen. Initially, the electrochemical oxidation of DMBA at the glassy carbon and pencil graphite electrodes in non-aqueous media (dimethylsulphoxide with lithium perchlorate) was studied by cyclic voltammetry. DMBA was irreversibly oxidized in two steps at high positive potentials, resulting in the ill-resolved formation of a couple with a reduction and re-oxidation wave at much lower potentials. Special attention was given to the use of adsorptive stripping voltammetry together with a medium exchange procedure on disposable pencil graphite electrode in aqueous solutions over the pH range of 3.0-9.0. The response was characterized with respect to pH of the supporting electrolyte, pre-concentration time and accumulation potential. Using square-wave stripping mode, the compound yielded a well-defined voltammetric response in acetate buffer, pH 4.8 at +1.15V (vs. Ag/AgCl)(a pre-concentration step being carried out at a fixed potential of +0.60 V for 360 s). The process Could be used to determine DMBA concentrations in the range 2-10 nM, with an extremely low detection limit of 0.194 nM (49.7 ng L-1). The applicability to assay of spiked human urine samples was also illustrated. Finally, the interaction of DMBA with fish sperm double-stranded DNA based on decreasing of the oxidation signal of adenine base was studied electrochemically by using differential pulse voltammetry with a pencil graphite electrode at the surface and also in solution. The favorable signal-to-noise characteristics of biosensor resulted in low detection limit (ca. 46 nM) following a 300-s interaction. These results displayed that the electrochemical DNA-based biosensor could be used for the sensitive, rapid. simple and cost effective detection of DMBA-DNA interaction. (C) 2009 Elsevier B.V. All rights reserved.