Browsing by Author "Meral, Ayfer"

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Can B-Type Natriuretic Peptide (Bnp) Levels Serve as an Early Predictor of Clinical Severity in Patients With Covid-19 Pneumonia
(Clin Lab Publ, 2022) Cilingir, Buket Mermit; Askar, Selvi; Meral, Ayfer; Askar, Muntecep
Background: COVID-19 has continued to aggressively spread and kill. The incidence of complications and associated mortality rates are high. Cardiac damage, which is related to survival, is one of these. The purpose of this study is to assess the role of BNP, a cardiac biomarker, in predicting mortality in COVID-19. Materials and Methods: This single-center, prospective observational study was performed from July to September 2020 in a tertiary university hospital designated for the treatment of COVID-19 patients. Patients whose diagnoses were confirmed with real-time polymerase chain reaction (RT-PCR) tested nasopharyngeal swabs and with thoracic computed tomography (CT) findings compatible with COVID-19 pneumonia were included in the study. All clinical and laboratory data were obtained within the first 24 hours of hospital admission. To determine the risk of in-hospital death, patients were followed from admission until their discharge (1 to 15 days). The primary outcome was in-hospital death, defined as the case-fatality ratio. Results: Among all biomarkers that were included in the multivariate analysis only high BNP levels was independently associated with mortality [Mean 1.012, 95% CI (1.005 -1.02 pg/mL) (p = 0.002)]. Mortality was found to be significantly associated with older age and higher BNP, LDH, AST, HGB, PLT, ferritin, D-dimer, and CRP levels. In addition, mortality was found to be higher with longer duration of hospitalization (p = 0.041). Conclusions: Our fundamental goal for COVID-19 is to determine whether the hospitalized patients are in the mortality risk group at an early stage of disease. Adding measurement of BNP levels to routine laboratory tests for COVID-19 may be a practical approach to determine the patients with a high risk of mortality. (Clin. Lab. 2022;68:xx-xx. DOI: 10.7754/Clin.Lab.2021.210602)
Erythropoietin Shows Gender Dependent Positive Effects on Social Deficits, Learning/Memory Impairments, Neuronal Loss and Neuroinflammation in the Lipopolysaccharide Induced Rat Model of Autism
(Churchill Livingstone, 2020) Solmaz, Volkan; Erdogan, Mumin Alper; Alnak, Alper; Meral, Ayfer; Erbas, Oytun
We aimed to evaluate the effects of EPO in the lipopolysaccharide (LPS) induced rat model of autism in terms of social deficits, learning and memory impairments, as well as their neurochemical correlates. Sixteen female Sprague Dawley rats randomly distributed into two equel groups, then were caged with fertile males for mating. At the 10th day of pregnancy, 0.5 ml %0,9 NaCl saline was given to first group, 100 mu g/kg LPS was given to second group to induce autism. On postnatal 21th day, forty-eight littermates were divided into four groups as; 8 male, 8 female controls, 16 male and 16 female LPS-exposed. Then, LPS groups were also divided in to two groups as saline (1 mg/kg/day) and EPO 600 U/kg/day groups, and animals were treated 45 days. At 50th day, after behavioral evaluations, brain levels of TNF-alpha, nerve growth factor (NGF) were measured. Histologically, hippocampal neuronal density and GFAP expression were assessed. Three-chamber sociability and social novelty test, passive avoidance learning test were revealed significant differences among the EPO and control groups. Histologically, hippocampal CAl & CA3 regions displayed significant alterations regarding gliosis (GFAP-positive cells) and regarding frontal cortical thickness in EPO groups compare to controls. Biochemical measurements of the brain levels of TNF-alpha and NGF levels showed significant differences between controls and EPO groups. According to our findings EPO treatment has beneficial effects on ASD-like symptoms, learning and memory processes, neuronal loss and neuroinflammation in the LPS induced rat model of autism, with some gender differences through inflammatory and neurotrophic pathways.
Protective Effect of Dapagliflozin on Colistin-Induced Renal Toxicity
(Mre Press, 2021) Bora, Ejder Saylav; Erdogan, Mumin Alper; Meral, Ayfer; Karakaya, Zeynep; Erbas, Oytun
Objectives: Multiple-drug resistance to Gram-negative bacteria has increased significantly in recent years. Colistin is increasingly used as a last line of defense against these bacteria. However, colistin has been associated with nephrotoxicity in experimental animals. This study explores the protective effect of dapagliflozin in a rodent model of nephrotoxicity. Material Method: The present study includes a total of 24 male rats, of which 16 were given a single 20 mg/kg dose of colistin (Colimycin 150 mg/mL) intravenously to induce renal toxicity. The remaining eight rats were given no drugs in order to serve as the control, Group A. The 16 rats treated with colistin were then divided into two groups. Rats in Group B received 0.9% NaCl saline solution at a dose of 30 mL/kg/day intraperitoneally (i.p.) and 10 mg/kg/day dapagliflozin (Forziga 10 mg) via oral gavage. Those in Group C received 0.9% NaCl saline solution at an i.p. dose of 30 mL/kg/day. Both saline and dapagliflozin were administered as described over the course of ten days. The animals were euthanized and blood samples were taken by cardiac puncture for further analysis. Their kidneys were removed for histopathological and biochemical examination. Results: Levels of creatinine, BUN, KIM-1, and MDA were significantly increased in the 16-rat (Groups B and C) treatment group, in comparison to the control group; however, these biomarkers were significantly normalized in Group B, which had received dapagliflozin in addition to saline. The GSH levels of Group C showed significant decline when compared to those of the control group, and were significantly normalized in Group B. Histologically, in Group 2, we observed severe tubular dilatation and tubular epithelial cell injury in comparison to the control group. These severe anatomical changes were decreased in Group B. Conclusion: Apart from its positive effect on glucose regulation, which is the usual purpose of dapagliflozin, we observed that in colistin-induced nephrotoxicity, it decreases oxidative stress by inhibiting SGLT-2, and has restorative effects in terms of histopathology and biochemistry. These findings offer hope that the use of dapagliflozin may be protective for contrast nephropathy, which causes renal tubule damage through oxidative mechanisms. Future studies will further clarify the mechanistic action of colistin and dapagliflozin, and may support the hypothesis that dapagliflozin can be used as an adjunctive therapy in all nephrotoxic conditions.
The Investigation of Lipid Peroxidation and Asymmetric Dimethylarginin Levels in Peritoneal Dialysis Patients Treated With Different Dialysis Solotions
(2010) Meral, Ayfer; Şekeroğlu, M. Ramazan
Böbrek fonksiyonlarında bozulma sonucu, son dönem böbrek yetmezliği hastalarında birçok patalojik bozuklukla beraber ateroskleroz ve kardiyovasküler bozukluklar da oluşmaktadır. Asimetrik dimetilarginin (ADMA), nitrik oksit sentazın endojen inhibitörüdür. Nitrik oksit sentezini azaltarak koroner arter hastalık gelişimi için bir risk faktörü olduğu kabul edilmektedir. Bu çalışmada periton diyalizi ile tedavi edilen hastalarda farklı iki diyaliz solüsyonu (Physioneal® ve Dianeal®) kullanımının kardiyovasküler risk faktörleri ve lipid peroksidasyonu üzerine etkilerinin karşılaştırılması amaçlandı. Physioneal® marka periton diyalizi solüsyonu ile tedavi gören 14 hasta çalışma grubuna alındı. Bu hastalardan öncelikle kan numuneleri alındı (Grup- 1) ve daha sonra aynı hastalarda tedaviye bir ay süreyle Dianeal® marka periton diyalizi solüsyonu ile devam edildi. Bu sürenin sonunda hastalardan yine kan numuneleri alındı (Grup- 2). Sonra hastaların tedavisinde tekrar Physioneal® solüsyonuna dönülerek bir aylık sürenin sonunda üçüncü kez kan numuneleri alındı (Grup- 3). Ayrıca hasta grubu ile yaş ve cinsiyet yönüyle eşleştirilmiş 16 sağlıklı şahıstan kontrol grubu oluşturuldu. Çalışmaya alınan bütün deneklerde serum ADMA, homosistein (Hcy), malondialdehit (MDA), hsCRP, vitamin B6, vitamin B12 ve folik asit seviyeleri ölçüldü. Her üç periton diyaliz grubunun serum ADMA seviyeleri (Grup-1: 0.527±0,112 ?mol/L; Grup-2: 0.637±0.14 ?mol/L; Grup-3: 0.54±0,113 ?mol/L) kontrol grubundan (0,423±0,105 ?mol/L) yüksek bulunurken, Grup-2'nin ADMA seviyesi ise, Grup-1 ve 3'ten daha yüksekti (p<0.05). Ayrıca bütün gruplarda ADMA ile Hcy arasında pozitif korelasyon bulundu (p<0,01). Hcy değerleri açısından, diyaliz gruplarımız ile kontrol grubu arasında anlamlı bir fark bulunmadı (p>0.05). Her üç diyaliz grubunda hsCRP, MDA, vitamin B12 ve folik asit seviyeleri kontrollerden yüksek bulunurken, vitamin B6 seviyeleri kontrolden düşüktü (p<0.05). Bu çalışmanın sonuçları; sürekli ayaktan periton diyalizi (SAPD) hastalarında aterosklerotik risk faktörlerinden serum ADMA seviyeleri ile hsCRP düzeylerinin ve oksidatif stresin kontrollere göre artmış olduğunu göstermiştir. Ayrıca Physioneal® solüsyonu ile tedavinin Dianeal®' e göre ADMA seviyelerinde daha az artışa neden olduğunun gösterilmesi, SAPD'li hastalarda fizyolojik periton diyalizi solüsyonu kullanımının ateroskleroz gelişimini azaltmada daha yararlı olabileceğini düşündürmektedir.Anahtar Sözcükler: Asimetrik dimetilarginin, kardiyovasküler risk faktörleri, periton diyalizi, lipid peroksidasyonu