Browsing by Author "Meral, I"

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Blood, Slaughter and Carcass Characteristics of Male Turkeys Following Quantitative Feed Restriction Between 8 To 16 Weeks of Age
(indian veterinary Journal, 2003) Gunduz, H; Meral, I; Ak, I; Mert, N
Comparative Study of Total Protein, and Total and Lipid-Associated Serum Sialic Acid Levels in Patients With Type 2 Diabetes Mellitus
(Wiley-liss, 2003) Ekin, S; Meral, I; Gunduz, H; Mert, N
The aim of the present study was to investigate the serum total protein (TP), total sialic acid (TSA), lipid-associated sialic acid (LSA), LSA/TP, and LSA/TP values in type 2 diabetes mellitus (DM) patients. Two study groups (healthy controls and type 2 DM subjects) were examined. For the type 2 DM group, 120 patients (60 females and 60 males) who had been diagnosed and treated for type 2 DM in the Yuzuncu Yil University Hospital, Van, Turkey, were selected consecutively. Forty healthy individuals (20 females and 20 males) were selected from hospital staff and other outpatient clinics to serve as the control group. They were matched for age, sex, body mass index, and smoking status. None of the participants had taken vitamin or mineral supplements for at least 2 weeks before sampling. To determine serum glucose, TP, TSA, and LSA levels, blood samples were drawn after all of the subjects fasted overnight. It was found that diabetics had higher TSA, LSA, TSA/TP, and LSA/TP levels than controls. However, the TP levels were not significantly different between the groups. Our results showed that TSA, LSA, TSA/TP, and LSA/TP have interactive connections with DM. These parameters can be used as a diagnostic index for patients with DM. J. Clin. Lab. Anal. 17:124-126, 2003. (C) 2003 Wiley-Liss, Inc.
Digoxin- and Monensin-Induced Changes of Intracellular Ca2+ Concentration in Isolated Guinea-Pig Ventricular Myocyte
(Wiley, 2002) Meral, I; Hsu, W; Hembrough, FB
This study was undertaken to determine the possible mechanisms of actions of monensin and digoxin by using isolated guinea-pig ventricular myocytes. Since Ca2+ is the major signal for triggering contraction of cardiac muscle, the objective of this study was to determine whether monensin and digoxin affect the [Ca2+]i of cardiac myocytes and if so is this effect due to an increase in [Na+] i. Three different concentrations of digoxin ( 0.3, 1 and 3 mu mol/l) and three different concentrations of monensin ( 0.3, 1 and 3 mu mol/l) were used. Each treatment was monitored for two hours by using computerized fluoroscopy. Both digoxin and monensin increased the [Ca2+] i and accelerated the onset time of [ Ca 2+] i increase in a dose- dependent manner. Normal myocytes ( loaded with fura-2 for 30 min before the treatment) were also compared with 'weakened' myocytes (loaded with fura-2 for 3 h before the treatment to create a 'weakened' condition). It was found that although 0.3 mu mol/l monensin and digoxin did not change the [ Ca 2+] i in normal myocytes, they increased the [Ca2+]i in 'weakened' myocytes. Finally, a Na+- free medium was used to demonstrate the effect of [Na+](o) on both monensin- and digoxin-induced increases in [ Ca 2+] i. It was found that digoxin did not increase the [ Ca 2+] i in the Na+- free medium. Although monensin increased the [ Ca 2+] i in the Na+- free solution, this increase was not as large as in the Na+- containing medium. The results of the study led to the conclusion that the positive inotropic effect of digoxin depends on [ Na+] o. However, monensin increases [ Ca 2+] i in Na+- dependent and -independent ways. An addition conclusion was that 'weakened' myocytes are more sensitive to the monensin and digoxin treatment than normal myocytes.
Effect of Nigella Sativa L. on Heart Rate and Some Haematological Values of Alloxan-Induced Diabetic Rabbits
(Scandinavian Federation Laboratory Animal Science, 2004) Meral, I; Donmez, N; Baydas, B; Belge, F; Kanter, M
This study was designed to investigate the effect of an extract of Nigella sativa L. on the heart rate and some haematological values in alloxan-induced diabetic rabbits. Fifteen New Zealand male rabbits were divided into three experimental groups: control, diabetic and N. sativa L.-treated diabetic. At the end of the experimental period (2 months), animals in all three groups were fasted for 12 hours and blood samples were taken for the determination of glucose levels, RBC and WBC (red and white blood cell) counts, packed cell volume (PCV), and haemoglobin (Hb) concentration. Heart rates were also measured by a direct-writing electrocardiograph before the blood withdrawals. It was found that N. sativa L. treatment increased the lowered RBC and WBC counts, PCV and neutrophil percentage in diabetic rabbits. However, the WBC count of the X sativa L. treated diabetic group was still lower than the control. N. sativa L. treatment also decreased the elevated heart rate and glucose concentration of diabetic rabbits. It is concluded that oral X sativa L. treatment might decrease the diabetes-induced disturbances of heart rate and some haematological parameters of alloxan-induced diabetic rabbits.
Effect of Nigella Sativa on Glucose Concentration, Lipid Peroxidation, Anti-Oxidant Defence System and Liver Damage in Experimentally-Induced Diabetic Rabbits
(Blackwell verlag Gmbh, 2001) Meral, I; Yener, Z; Kahraman, T; Mert, N
This Study was carried out to investigate whether Nigella sativa could decrease the lipid peroxidation, increase the anti-oxidant defence system and also prevent the lipid-peroxidation-induced Ever damage in experimentally induced diabetic rabbits. Fifteen New Zealand male rabbits were divided into three experimental groups: control, diabetic and diabetic and N. sativa-treated. The diabetes mellitus (DM) was induced in the rabbits using 150 mg/kg of 10% alloxan. The diabetic + N. sativa-treated group was given extract of N sativa seeds orally every day for 2 months after induction of D-M. At the end of the 2-month experiment, blood samples were collected to measure malondialdehyde (MDA), glutathione (GSH), ceruloplasmin and glucose concentration, and livers were harvested for histopathological analysis, Treatment with N sativa decreased the elevated glucose and MDA concentrations, increased the lowered GSH and ceruloplasmin concentrations, and prevented lipid-peroxidation-induced Ever damage in diabetic rabbits. It was concluded that N sativa might be used in diabetic patients to prevent lipid peroxidation, increase anti-oxidant defence system activity, and also prevent liver damage.
Effect of Short-Term Hypothermia on Lipid Peroxidation and Antioxidant Enzyme Activity in Rats
(Wiley-blackwell, 2002) Dede, S; Deger, Y; Meral, I
This experiment was carried out to determine the effect of short-term hypothermia on blood malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G-6-PD) concentrations in rats. Twenty Sprague-Dawley rats were used weighing 180-200 g and on average 3.5 months old. They were randomly divided into two experimental groups: control (without cooling) and hypothermic (with cooling). The rats of the hypothermic group were cooled by immersion into cold water (10-12 degrees C), and the control rats were immersed into water of body temperature (37 degrees C) up to the neck without using any anaesthetic or tranquilizer for 3 min Rectal body temperatures of both groups were measured and blood samples to analyse MDA, GSH, SOD, GSH, GSH-Px and G-6-PD were collected immediately after the treatment. It was found that the MDA level was higher and the GSH and G-6-PD levels were lower in the hypothermic group than those in the controls. There was no difference between the control or hypothermic group regarding SOD or GSH-Px levels. It is concluded that acute hypothermia increased the lipid peroxidation and decreased the GSH and G-6-PD levels in rats.
Effects of a Gnrh Agonist on Oocyte Number and Maturation in Mice Superovulated With Ecg and Hcg
(Elsevier Science inc, 2004) Kanter, M; Yildiz, C; Meral, I; Koc, A; Tasal, I
The objective was to investigate the effects of a gonadotropin-releasing hormone agonist (GnRH) on ovulation rate and the number and maturation of oocytes in mice superovulated with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Thirty 3-month-old BALB/C female mice (weight: 25-30 g) were assigned to three experimental groups: control, superovulated, and superovulated with GnRH pretreatment (n = 10 per group). Control mice received an i.p. injection of 0.1 ml physiological saline solution. Superovulation was induced with 5 IU eCG (i.p.) and 5 IU hCG 48 It later. Mice in the superovulated with GnRH pretreatment group were given GnRH (20 mg/kg Fertirelin, i.m.), 24 h before superovulation. Thirteen hours after hCG administration, mice were sacrificed by cervical dislocation and blood samples were collected to determine serum progesterone concentration (by radioimmunoassay). Ovaries and oviducts were also harvested to enumerate corpora lutea and cumulus-enclosed oocytes. Progesterone concentrations were not significantly different among groups. The oocyte number and the maturation, ovulation rate, and the number of corpora lutea were higher in GnRH-treated mice than both controls and superovulated mice. In conclusion, GnRH given 24 h before superovulation with eCG-hCG increased the number and maturation of oocytes and the rate of ovulation in mice. (C) 2003 Elsevier Inc. All rights reserved.
Effects of Cadmium Exposure on Morphological Aspects of Pancreas, Weights of Fetus and Placenta in Streptozotocin-Induced Diabetic Pregnant Rats
(Humana Press inc, 2003) Kanter, M; Yoruk, M; Koc, A; Meral, I; Karaca, T
This study was designed to evaluate the effects of Cd exposure on morphological aspects of beta-cell and weights of fetus and placenta in streptozotocin (STZ)-induced diabetic pregnant rats. Ninety-nine virgin female Wistar rats (200-220 g) were mated with 33 males for at least 12 h. From the onset of pregnancy, the rats were divided into four experimental groups (control, Cd treated, STZ treated, and Cd+STZ treated). The Cd-treated group was injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl, starting at the onset of pregnancy throughout the experiment. Diabetes was induced on the 13th d of pregnancy by a single intraperitoneal injection of STZ in STZ-treated group. In addition to the daily injection of Cd, a single intraperitoneal injection of STZ was also given on the 13th d of pregnancy in the Cd+STZ-treated group. The rats received the last injection 24 h before being sacrificed and 10 randomly selected rats in each group were sacrificed on the 15th and 20th d of pregnancy. Blood samples were taken for the determination of the serum glucose and insulin levels. Maternal pancreases, fetuses, and placentas of sacrificed rats in all groups were harvested (fetal pancreas was also harvested only on the 20th d of pregnancy) for morphological and immunohistochemical examinations. Cd exposure alone caused a degeneration, necrosis, and weak degranulation, but Cd exposure with STZ caused a severe degeneration, necrosis, and degranulation in the beta-cells of the pancreatic islets. No morphological or immunohistochemical differences were found in beta-cells of fetal pancreatic islets of control or other treatment groups. Cd exposure alone also decreased the fetal and placental weights. The administration of STZ alone, on the other hand, increased the placental weight. Cd, STZ, and Cd+STZ administration increased the glucose and decreased the insulin level. The increase in glucose and decrease in insulin levels were higher when Cd and STZ were given together. All of these changes were more severe on the 20th d than those on the 15th d of the pregnancy. It is concluded that Cd exposure during pregnancy may reduce the birth and placental weights and produce necrosis, degeneration, and degranulation in beta-cells of pancreatic islets, causing an increase in the serum glucose level. These changes might be severe in diabetic pregnant mothers.
The Effects of Carbon Tetrachloride (Ccl4) Induced Experimental Acute and Chronic Intoxication on Histological Structure of Liver and Some Hematological Values and Electrocardiogram in Rabbits
(Scientific Technical Research Council Turkey, 1999) Çinar, A; Yörük, M; Meral, I; Kiliçalp, D; Koç, A; Ertekin, A
This paper reports an investigation of the effects of acute and chronic carbon tetrachloride intoxications on the histologic structure of the liver and hematological values and electrocardiograms in rabbits. The rabbits were divided into 3 groups of 7. The first group was the control group, the second group was acutely intoxicated and the third group was chronically intoxicated. In the acutely intoxicated group, caryorexsis, along with necrosis was observed in the hepatocyte of the centrolobular area of the liver. In the chronically intoxicated group, connective tissue mass around the V. centralis increased. This increase of connective tissue caused chirrosis. Hematological values of the groups were as follows: red blood cells 5.86+/-0.6, 5.95+/-1.5, 4.26+/-0.7 billion/mm(3); white blood cell: 6.9+/-1.35, 5.90+/-0.8, 4.0+/-2.44 thousand/mm(3); hemoglobin concentration: 12.7+/-0.9, 13.6+/-1.5, 11.8+/-1.2 gr/dl; hematocrit value: 41.8+/-3.3, 44.6+/-4.9, 33.0+/-4.4%; sedimentation: 5.2+/-1.6, 8.0+/-2.8, 10.2+/-1.3 45 degrees/1 hours respectively. The formula leukocyte was as follows: in the control group: neutrophil 34+/-5.3%, eosinophil 0.6+/-0.8%, basophil 0.2+/-0.4%, lymphocyte 62+/-5.02%, monocyte 3.2+/-0.97%. In the acutely intoxicated group: neutrophil 7.75+/-1.1%; eosinophil 1+/-0%; basophil 0+/-0%; lymphocyte 88.5+/-1.3%; monocyte 3.5+/-1.8%. In the chronically intoxicated group: neutrophil 60+/-8.51%; eosinophil 1+/-0.71%; basophil 0+0%: lymphocyte 35.75+/-7.33%; monocyte 3.2+/-0.83%. The extremity leads and 2 vertical and 1 horizontal chest leads were used in the electrocardiograph of the rabbits, All waves were observed in all derivations. The P and T waves were negative in the III and aR leads and positive in the other leads, The amplitudes of waves decreased (hypo voltage) as a result of intoxication. In the groups, the heart rates were 219+/-21 in the control. 215+/-17 in the acutaly intoxicated, 201+/-35 per minute in the chronically intoxicated and the mean electrical axes of the heart rate were 45 degrees+/-20, 50 degrees+/-27. 35 degrees+/-20 respectively. Statistical comparisons were carried out using analysis of variance and Q test.
Effects of Diet Treatment on Some Biochemical and Physiological Parameters in Patients With Type 2 Diabetes Mellitus
(Tohoku Univ Medical Press, 2003) Meral, I; Kacmaz, R
The aim of the present study was to investigate the effect of diet treatment on serum glucose, triglyceride (TG), total cholesterol (TC), high density lipoprotein-(HDL) cholesterol, low density lipoprotein-(LDL) cholesterol and very low density lipoprotein-(VLDL) cholesterol levels, systolic and diastolic blood pressure and electrocardiograms (ECGs) in patients with type 2 diabetes mellitus (DM). Twenty healthy subjects (mean age 45.9 +/- 3.7 years) and newly diagnosed patients with type 2 diabetes prior to receiving diet treatment (mean age 47.6 +/- 6.2 years) were included in this study. Diabetic patients were given a standard dietary treatment that was composed of 50% to 55% carbonhydrate and 30% fat (1200 kcal for women and 1600 kcal for men) for 2 months. No diet treatment was applied for control. For both groups serum glucose, TG, TC, HDL-cholesterol, LDL-cholesterol and VLDL-cholesterol levels, systolic and diastolic blood pressure and ECGs were measured at the beginning and end of the diet treatment. Although diet treatment decreased the elevated serum glucose in diabetic patients, it still remained higher than that in the controls. Diet treatment also decreased the elevated TG and VLDL-cholesterol in diabetic patients to control values. Although heart rate and systolic blood pressure were higher, diastolic blood pressure was not different in diabetic patients than those in controls. Ventricular hypertrophy was also observed in ECGs of 10% of diabetic patients. Diet treatment normalized all of these findings, except systolic blood pressure. This study showed that diet treatment could not normalize the high systolic blood pressure in type 2 DM. Thus, an effective way of controlling blood pressure should be taken to improve healing in DM.
Effects of Different Concentrations of Monensin on the Contractility Changes of Guinea-Pig Papillary Muscle
(Scientific Technical Research Council Turkey, 1998) Meral, I
Effects of different concentrations of monensin on contraction amplitude (CA) which is the contraction force (CF), initial contraction velocity (ICV), average contraction velocity (ACV) and initial relaxation velocity (IRV) of guinea-pig papillary muscle were studied by using male guinea-pigs. Hearts were removed and papillary muscles were isolated from the right ventricles. The muscles were then attached to a capacitance transducer, electrically connected to a Beckman recorder and the isometric contractions of guinea-pig papillary muscles were recorded. The five hours of measurement in this experiment were divided into five periods (T0 was equilibration, T1, T2, T3 and T4 were respectively one, two, three and four hours after drug administration). Two mu mol/l monensin did not change any parameters at any time period. Although 12 mu mol/l monensin did not change the CA, ACV or IRV at any time period, it increased the ICV at T2. Seven mu mol/l monensin, on the other hand, increased the CA and IRV at T2, T3 and T4 and the ICV and ACV at T1, T2, T3 and T4. It is concluded that the effect of monensin in guinea-pig papillary muscles is dose dependent, It is also concluded that although the therapeutic range of monensin is very narrow, it should be considered as an alternative drug for the treatment of congestive heart failure (CHF). However, more studies are needed to domenstrate the effectiveness of monensin on the tratment of congestive heart failure.
Effects of Different Concentrations of Monensin on the Electrocardiogram and the Serum Ion Balance of the Rabbit
(Scientific Technical Research Council Turkey, 1998) Meral, I; Çinar, A; Bayiroglu, F; Aslan, SO
The present study was planned to investigate the effects of two different concentrations (10 and 40 mg/kg) of monensin, carboxylic ionophore antibiotic, on the ECG and the serum Na+ and K+ concentrations of rabbits. Fifteen male rabbits, about 2 kg and 1 year old, were used. ECGs were recorded by a direct writing electrocardiograph. Blood samples for measuring serum Na+ and the K+ concentrations were collected and the leads I, ii, II, aVR, aVL and aVF were recorded before and 6 hours after monensin was given. The durations and amplitudes of waves on the trace were measured in lead II and electrical axis also measured in leads 1 and III. We found that the effects of monensin on ECG and the serum Na+ and the K+ concentrations were dose dependent. In low doses (10 mg/kg) it produced a sinusoidal tachycardia by decreasing the time interval between two impulse production by sinoatrial node without changing the serum ion balance. However, in high doses (40 mg/kg) it produced a sinusoidal tachycardia with increasing the serum K+ and decreasing the serum Na+ concentrations.
Effects of Increasing Zinc Supplementation in Drinking Water on Growth and Thyroid Gland Function and Histology in Broiler Chicks
(M H Schaper Gmbh Co Kg, 2002) Dönmez, HH; Karsli, MA; Meral, I; Dönmez, N; Simsek, N
The aim of the study was to examine the effects of increasing zinc supplementation on growth, feed efficiency and thyroid function and histology in broiler chicks. Sixty new born male broiler chicks were randomly allotted into one of four treatment groups and fed for 60 d. Zinc (Zn) was added into drinking water at the levels of 0, 125, 500, and 1000 mg Zn/L. Body weight gain were significantly higher and feed efficiency were significantly lower in chicks supplemented with 125 mg Zn/L compared with chicks supplemented with 500 or 1000 mg Zn/L at the end of the experiment. Serum Zn concentration linearly increased with the increasing level of Zn intake. Serum triiodothyronine and thyroxine levels and the diameters of follicles of thyroid gland were significantly reduced with high levels (500 and 1000 mg Zn/L) of Zn intake at the end of the experiment. It was concluded that chick receiving 1000 mg Zn/L as ZnSO4 . 7H(2)O in drinking water showed signs of Zn toxicity.
Effects of Melatonin on Lipid Peroxidation and Anti-Oxidant Enzyme Activity in Rats With Experimentally Induced Hyperthyroidism
(Wiley, 2005) Baydas, B; Meral, I
1. The present study was designed to investigate the effects of high-dose melatonin on lipid peroxidation and anti-oxidant enzyme activity in rats with experimentally induced hyperthyroidism. 2. Twenty-four albino male rats, weighing 240-260 g, were randomly allotted into one of three experimental groups (control, hyperthyroid and hyperthyroid + melatonin treatment), with each group containing eight animals. Hyperthyroidism was induced by a daily with i.p. injection of 200 mu g L-thyroxine for 30 days. In addition to L-thyroxin treatment, rats in the hyperthyroid + melatonin treatment group were also given daily i.p. injections of 10 mg/kg melatonin on the last 10 days of L-thyroxine treatment. Control animals received injections of an equivalent volume of saline solution. Rats received the last injection 24 h before being killed. 3. At the end of the experiment, rats in all three groups were fasted for 12 h and killed by cardiac puncture under ether anaesthesia. Blood samples were taken for the determination of malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) levels and concentrations of tri-iodothyronine (T-3) and thyroxine (T-4). 4. It was found that MDA and SOD levels and concentrations of T-3 and T-4 were higher and the GSH level was lower in rats with hyperthyroidism compared with controls. Melatonin treatment decreased the elevated MDA and SOD levels and increased the lowered GSH level to control levels in rats with hyperthyroidism, but did not ameliorate the concentrations of T-3 and T-4. 5. It was concluded that high-dose melatonin treatment may decrease the hyperthyroidism-induced disturbances of lipid peroxidation and anti-oxidant enzyme activity and oxidative damage.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats
(Wiley, 2003) Kanter, M; Meral, I; Dede, S; Cemek, M; Ozbek, H; Uygan, I; Gunduz, H
This study was designed to investigate the effects Nigella sativa L. (NS) and Urtica dioica L. (UD) on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in carbon tetrachloride (CCl4)-treated rats. A total of 60 healthy male Sprague-Dawley rats were utilized in this study. The rats were randomly allotted into one of four experimental groups: A (CCl4 -only treated), B (CCl4 + UD treated), C (CCl4 + NS treated) and D (CCl4 + UD + NS treated), each containing 15 animals. All groups received CCl4 [0.8 ml/kg of body weight, subcutaneously, twice a week for 90 days starting day 1]. In addition, B, C and D groups also received daily intraperitoneal injections of 0.2 ml/kg NS or/and 2 ml/kg UD oils for 45 days starting day 46. Group A, on the other hand, received only 2 ml/kg normal saline solution for 45 days starting day 46. Blood samples for the biochemical analysis were taken by cardiac puncture from five randomly chosen rats in each treatment group at beginning, at 45th and at 90th day of the experiment. The CCl4 treatment for 45 days increased the lipid peroxidation and liver enzymes, and also decreased the antioxidant enzyme levels. NS or UD treatments (alone or combination) for 45 days starting day 46 decreased the elevated lipid peroxidation and liver enzyme levels and also increased the reduced antioxidant enzyme levels. Live weights of the rats decreased in group A, and increased in groups B, C and D. It is concluded that NS and UD decrease the lipid peroxidation and liver enzymes, and increase the antioxidant defence system activity in the CCl4-treated rats.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats (Vol 50, Pg 264, 2003)
(Blackwell verlag Gmbh, 2003) Kanter, M; Meral, I; Dede, S; Gunduz, H; Cemek, M; Ozbek, H; Uygan, I
Effects of Nigella Sativa L. and Urtica Dioica L. on Selected Mineral Status and Hematological Values in Ccl4-Treated Rats
(Humana Press inc, 2003) Meral, I; Kanter, M
This study was designed to investigate the effects of Nigella sativa L. (NS), known as black seed, or/and Urtica dioica L. (UD), known as stinging nettle root, treatments on serum Na, K, Cl, and Ca levels and some hematological values of CCl4-treated rats. Sixty healthy male Sprague-Dawley rats, weighing 250-300 g, were randomly allotted into 1 of 4 experimental groups: A (CCl4-only treated), B (CCl4 + UD treated), C (CCl4 + NS treated), and D (CCl4 + UD + NS treated), each containing 15 animals. All groups received CCl4 (0.8 mL/kg of body weight, subcutaneously, twice a week for 90 d starting d 1). In addition, B, C, and D groups also received the daily ip injection of 0.2 mL/kg NS and/or 2 mL/kg UD oils for 45 d starting d 46. Group A, on the other hand, received only 2 mL/kg normal saline solution for 45 d starting d 46. Blood samples for the biochemical analysis were taken by cardiac puncture from five randomly chosen rats in each treatment group at the beginning, d 45, and d 90 of the experiment. The CCl4 treatment for 45 d significantly (p < 0.05) increased the serum K and Ca and decreased (p < 0.05) the red blood cell count (RBC), white blood cell count (WBC), packed cell volume (PCV), and Hb levels without changing (p > 0.05) the serum Na and Cl levels. NS or UD treatments (alone or combination) for 45 d starting d 46 significantly (p < 0.05) decreased the elevated serum K and Ca levels and also increased (p < 0.05) the reduced RBC, WBC, PCV, and Hb levels. It is concluded that NS and/or UD treatments might ameliorate the CCl4-induced disturbances of anemia, some minerals, and body's defense mechanism in CCl4-treated rats.
Effects of Nigella Sativa L. on Serum Concentrations of Thyroid Hormones, Thyroid Stimulating Hormone and Glucose in Alloxaninduced Diabetic Rabbits
(veterinary Ireland, 2003) Meral, I; Yener, Z; Ozbek, H; Ustun, R
This study was designed to investigate the effect of an extract of Nigella sativa L. (NS) on the histology and function of the thyroid glands in alloxan-induced diabetic rabbits. Fifteen New Zealand male rabbits were divided into three experimental groups: control, diabetic and NS-treated diabetic. At the start of the experiment, a single intravenous injection of 10% alloxan (150 mg/kg body weight (bwt)) was given to produce diabetes mellitus (DM) in the diabetic and NS-treated diabetic groups. After DM was confirmed in the two groups, rabbits in the NS-treated group were given oral doses of 20 ml/kg bwt of the NS extract every day for two months. Then Mood samples were taken from all 15 rabbits to measure the serum concentrations of glucose, triiodothyronine (T-3), thyroxine (T-4) and thyroid stimulating hormone (TSH). The rabbits were killed and tissue samples were taken from the thyroid glands for histopathological analysis. It was found that NS treatment decreased the elevated glucose concentrations, and raised the lowered serum triiodothyronine concentrations in diabetic rabbits. There were no significant differences between groups in the histology of the thyroid glands or in the concentrations of serum T, and serum TSH. It was suggested that oral NS treatment might decrease the diabetes-induced disturbances of thyroid hormone metabolism.
Effects of Oral Zinc and Magnesium Supplementation on Serum Thyroid Hormone and Lipid Levels in Experimentally Induced Diabetic Rats
(Humana Press inc, 2002) Baydas, B; Karagoz, S; Meral, I
This study was designed to investigate the effects of oral zinc and magnesium supplementation on serum thyroid hormone and lipid levels in alloxan-induced diabetic rats. Thirty-two albino male rats, weighing 234 34 g, were divided into four experimental groups (control, diabetic, diabetic+zinc supplemented and diabetic+ magnesium supplemented). The experiment lasted for 60 d. The first 45 d of the experiment was the supplementation and last 15 d was the supplementation and diabetes-inducing period. Diabetic+zinc-supplemented and diabetic+magnesium-supplemented groups were given orally (by adding in their drinking water) 227 mg/L of zinc and 100 mg/kg body weight (bw) of magnesium, respectively throughout the experiment. Control and diabetic groups served as controls and did not receive zinc or magnesium supplementation. Diabetic, diabetic+zinc-supplemented, and diabetic+magnesium-supplemented groups were given a daily injection (ip) of 100 mg/kg bw of alloxan for 15 d starting on d 46 of the experiment. The control group was only injected with the same volume of isotonic NaCl as the diabetic group received. At the end of the of the experiment, rats in all four groups were fasted for 12 h and blood samples were taken from the heart under ether anesthesia for the determination of thyroid hormone, glucose, total cholesterol, and triglyceride concentrations. It was found that serum glucose, total cholesterol, and triglyceride concentrations were higher and serum T3 and T4 concentrations were lower in diabetic rats than those in the control group. Zinc supplementation did not change any parameter in diabetic rats. However, magnesium supplementation decreased the elevated total cholesterol and triglyceride concentrations of the diabetic rats to the control level. It was concluded that oral magnesium supplementation might decrease the diabetes-induced disturbances of lipid metabolism.
Erythropoietin Level and Some Blood Parameters in Cattle With Tropical Theileriosis
(indian veterinary Journal, 2004) Ceylan, E; Agaoglu, Z; Gul, Y; Meral, I; Dabak, M