Browsing by Author "Mert, N"

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Blood, Slaughter and Carcass Characteristics of Male Turkeys Following Quantitative Feed Restriction Between 8 To 16 Weeks of Age
(indian veterinary Journal, 2003) Gunduz, H; Meral, I; Ak, I; Mert, N
Changes in Erythrocyte Parameters of Fluorotic Sheep
(int Soc Fluoride Research, 2003) Yur, F; Belge, F; Mert, N; Yörük, I
The aim of this study was to investigate changes in oxidative stress parameters of erythrocytes in twenty 3-4 year-old fluorotic sheep as compared to twenty controls. Malondialdehyde (MDA) levels (mean +/- SD) were significantly elevated in erthyrocytes of the fluorotic sheep (2.51 +/- 0.16 nmol/mL) in comparison to the control sheep (1.32 +/- 0.13 nmol/mL). Erythrocyte Na+-K+ adenosine-5'-triphosphatase (Na+-K+ ATPase) activity in the fluorotic sheep (0.003 +/- 0.0005 mumol Pi/mg protein/h) was significantly lower than in the controls (0.005 +/- 0.0005 mumol Pi/mg protein/h), as was the activity of glucose-6-phosphate dehydrogenase (G6PD) (510 +/- 23.30 mU/g Hb) compared to the controls (1418 +/- 85.40 mU/g Hb). The results are explained by fluoride-induced oxidative stress reflected in elevated erythrocyte MDA levels that cause decreased enzyme activity of Na+-K+ ATPase and G6PD by affecting membrane structure.
Comparative Study of Total Protein, and Total and Lipid-Associated Serum Sialic Acid Levels in Patients With Type 2 Diabetes Mellitus
(Wiley-liss, 2003) Ekin, S; Meral, I; Gunduz, H; Mert, N
The aim of the present study was to investigate the serum total protein (TP), total sialic acid (TSA), lipid-associated sialic acid (LSA), LSA/TP, and LSA/TP values in type 2 diabetes mellitus (DM) patients. Two study groups (healthy controls and type 2 DM subjects) were examined. For the type 2 DM group, 120 patients (60 females and 60 males) who had been diagnosed and treated for type 2 DM in the Yuzuncu Yil University Hospital, Van, Turkey, were selected consecutively. Forty healthy individuals (20 females and 20 males) were selected from hospital staff and other outpatient clinics to serve as the control group. They were matched for age, sex, body mass index, and smoking status. None of the participants had taken vitamin or mineral supplements for at least 2 weeks before sampling. To determine serum glucose, TP, TSA, and LSA levels, blood samples were drawn after all of the subjects fasted overnight. It was found that diabetics had higher TSA, LSA, TSA/TP, and LSA/TP levels than controls. However, the TP levels were not significantly different between the groups. Our results showed that TSA, LSA, TSA/TP, and LSA/TP have interactive connections with DM. These parameters can be used as a diagnostic index for patients with DM. J. Clin. Lab. Anal. 17:124-126, 2003. (C) 2003 Wiley-Liss, Inc.
Correlation Between Biochemical Parameters and Production Traits in Merino Cross Sheep Ii -: Hemoglobin and Transferrin Types
(Scientific Technical Research Council Turkey, 2003) Mert, N; Gündüz, H; Akgündüz, V; Akgündüz, M
To determine the relationship between biochemical parameters and production traits in sheep, 111 of the animals raised in the Bandirma Sheep Research Institute were used as living materials. The blood samples of 31 Hampshire Down x Merino, 33 Dorset Down x Merino, 27 German Black Headed x Merino and 20 Border Leicester x Merino crosses were taken. Transferrin (Tf) and haemoglobin (Hb) types were determined. Some production traits such as greasy fleece weight, staple length, fiber diameter, ondulance and lamb birth weight were estimated. Important correlations between Hb AB and lamb birth weights (P less than or equal to 0.05), Hb AB and greasy fleece weight in SBA x Merino crosses (P less than or equal to 0.05), Hb AB and greasy fleece weight in Border Leicester x Merino crosses (P less than or equal to 0.05), Tf B type and greasy fleece weight in SBA x Merino and Border Leicester x Merino crosses (P less than or equal to 0.05) and Tf A type and lamb birth weight in SBA x Merino crosses (P less than or equal to 0.05) were also determined.
Correlation Between Biochemical Parameters and Production Traits in Merino Cross Sheep Iii -: Glucose, Alkaline Phosphatase, Ceruloplasmin
(Scientific Technical Research Council Turkey, 2003) Mert, N; Gündüz, H; Akgündüz, V; Akgündüz, M
A group of 111 sheep raised in the Bandirma Sheep Research Institute were used as living materials for this study. Blood samples from 31 Hampshire Down x Merino, 33 Dorset Down x Merino, 27 German Black Headed x Merino and 20 Border Leicester x Merino crosses were taken. Plasma glucose, alkaline phosphatase (ALP) and ceruloplasmin levels were determined. Some production traits such as greasy fleece weight, staple length, fiber diameter, ondulance, lamb birth weight, reproduction traits and the live weight gain of lambs were estimated. There were blood glucose differences between the breeds and an important correlation between greasy fleece weight and ALP and glucose levels.
Correlation Between Biochemical Parameters and Production Traits in Merino Crosses Sheep -: I -: Erythrocyte Potassium and Glutathione
(Scientific Technical Research Council Turkey, 2003) Mert, N; Gündüz, H; Akgündüz, V; Akgündüz, M
To determine the relationship between biochemical parameters and production traits in sheep, 111 sheep raised in Bandirma Sheep Research Institute were used as living material. Blood samples of 31 Hampshire Down x Merino, 33 Dorset Down x Merino, 27 German Black Headed x Merino and 20 Border Leicester x Merino crosses were taken. The levels and types of blood glutathione (GSH) and erythrocyte potassium (K+) were determined. Correlations between some production traits such as greasy fleece weight and lamb birth weight were estimated. Statistical significance was found in greasy fleece weight and high glutathione (GSH(H)) and low potassium (LK) SBA x Merino crosses, but in Dorset Down x Merino crosses only low glutathione (GSH(h)) type was shown to have significance with regards lamb birth weight.
Effect of Nigella Sativa on Glucose Concentration, Lipid Peroxidation, Anti-Oxidant Defence System and Liver Damage in Experimentally-Induced Diabetic Rabbits
(Blackwell verlag Gmbh, 2001) Meral, I; Yener, Z; Kahraman, T; Mert, N
This Study was carried out to investigate whether Nigella sativa could decrease the lipid peroxidation, increase the anti-oxidant defence system and also prevent the lipid-peroxidation-induced Ever damage in experimentally induced diabetic rabbits. Fifteen New Zealand male rabbits were divided into three experimental groups: control, diabetic and diabetic and N. sativa-treated. The diabetes mellitus (DM) was induced in the rabbits using 150 mg/kg of 10% alloxan. The diabetic + N. sativa-treated group was given extract of N sativa seeds orally every day for 2 months after induction of D-M. At the end of the 2-month experiment, blood samples were collected to measure malondialdehyde (MDA), glutathione (GSH), ceruloplasmin and glucose concentration, and livers were harvested for histopathological analysis, Treatment with N sativa decreased the elevated glucose and MDA concentrations, increased the lowered GSH and ceruloplasmin concentrations, and prevented lipid-peroxidation-induced Ever damage in diabetic rabbits. It was concluded that N sativa might be used in diabetic patients to prevent lipid peroxidation, increase anti-oxidant defence system activity, and also prevent liver damage.
Effects of Gestation and Lactation on Lipid Peroxidation and Antioxidant System in Sheep
(indian veterinary Journal, 2003) Yur, F; Dede, S; Deger, Y; Mert, N
Pregnancy is accompanied by increased energy demands of many body systems. Production of reactive oxygen species (ROS) is proportional to the rate of oxygen consumption. Therefore, a concomitant increase in the activity of antioxidants during pregnancy could be expected (Mover-Lev and Ar., 1997). Malondialdehyde (MDA) is a major breakdown product split off from lipid peroxides (Mihailovic et al., 2000). The antioxidant systems protect the cells against lipid peroxidation which is the basis of many pathologic processes (Bray and Bettger, 1990). Superoxide dismutase (SOD) are metalloenzyme, and important initial component in the cellular defense against oxygen toxicity (Fridovich, 1986). Glutathione peroxides (GPx) are enzymes that catalyze the reduction of organic hydroperoxides or hydrogen peroxide by glutathione (Knapen, 2000). Antioxidant vitamins like E, C and A protect the cells from damage against free oxygen radicals. The present study examined the level of MDA and antioxidative substances such as SOD, GPx, GSH, vit E, C, beta-carotene, retinol, during the gestation and, lactation period of sheep.
Effects of X-Ray Radiation on Lipid Peroxidation and Antioxidant Systems in Rabbits Treated With Antioxidant Compounds
(Humana Press inc, 2003) Deger, Y; Dede, S; Belge, A; Mert, N; Kahraman, T; Alkan, A
The aim of this study was to investigate the effects of supplemental antioxidant vitamins and minerals on lipid peroxidation and on the antioxidant systems in rabbits exposed to X-rays. The rabbits were divided into two experimental groups and one control group, each group containing seven rabbits. The first group (VG) received daily oral doses of vitamin E (460 mg/kg live weight) and vitamin C (100 mg/kg live weight). The second group (MG) was fed a mineral-enriched diet that contained 60 mg manganese chloride, 40 mg zinc sulfate, and 5 mg copper sulfate per kilogram of feed. The third group served as controls and received only a standard diet. Blood samples were obtained before and after the supplementation with vitamins or minerals, as well as before and after irradiation with a total dose of 550-rad X-rays. The blood samples were analyzed for their content of malondialdehyde (MDA), plasma vitamins C and E, retinol, reduced glutathione (GSH), and glutathione peroxidase activity (GPx). After irradiation, the control group showed increased levels of MDA and activity of GPx (p<0.05), whereas the levels of GSH, vitamin C, and vitamin E were decreased. In the VG, the concentration of MDA was lower (p<0.05), and the concentration of GSH and vitamins C and E were higher (p<0.05) when compared to controls. In the MG, the concentrations of MDA, GSH, vitamin C, and retinol were not affected by the mineral administration and radiation. The level of vitamin E in the MG increased with mineral administration (p<0.05), but decreased after irradiation (p<0.05). For the control group, the level of GSH was higher than in the two experimental groups. After irradiation, the VG animals had vitamin E and C levels that were higher than in MG and control groups (p<0.05). The activity of GPx was not affected by vitamin or mineral supplementation or by irradiation. We conclude that the supplementation with antioxidant vitamins and minerals may serve to reinforce the antioxidant systems, thus having a protective effect against cell damage by X-rays.
An Investigation on Blood Gases and Some Haematological-Biochemical Parameters in Experimentally Induced Liver Cirrhosis of Dogs
(indian veterinary Journal, 2003) Ertekin, A; Mert, N; Akgul, Y; Karaca, M; Akkan, HA; Keles, I
Levels of Some Trace Elements and Rheumatoid Factor in Sheep With Brucellosis
(Humana Press inc, 2004) Ekin, S; Kozat, S; Gunduz, H; Mert, N; Karakaya, C
Brucellosis is a zoonotic disease that is encountered in sheep rather frequently. In this study, 100 sheep diagnosed with brucellosis that had aborts and 40 healthy sheep were used as materials. Analyses for Cu, Zn, Fe, Cr, Ca, Mg, and K were performed with the atomic absorption spectrophotometric method on blood collected from vena jugularis of all the sheep and rheumatoid factor levels were determined by the nephelometry method. Although it was found that Cu, Ca, and rheumatoid factor values in the sera of the sheep with brucellosis were high when compared to the control group (p<0.001, p<0.05, p<0.001, respectively), their serum Zn values were low (p<0.05). No significant changes in serum Cr, Fe, K, and Mg levels were found.
Serum Copper, Zinc, Manganese, and Magnesium Status of Subjects With Chronic Fluorosis
(int Soc Fluoride Research, 2004) Meral, I; Demir, H; Gunduz, H; Mert, N; Dogan, I
This study was designed to investigate the serum copper (Cu), zinc (Zn), manganese (Mn), and magnesium (Mg) status in a group of subjects with chronic fluorosis. Fifteen subjects diagnosed with fluorosis who live near Van, Turkey in the fluoride-polluted volcanic Tendurek Mountain region (altitude ca. 2000 m) were randomly selected to represent the fluorotic group. Fifteen healthy individuals living near this region matched for age, sex, body mass index, and smoking status were selected as controls. None of the participants had taken any vitamin or mineral supplements for at least 2 weeks before blood samples were drawn after overnight fasting. Subjects with chronic fluorosis had lower serum Cu, Zn, Mn, and Mg levels than did the controls, and it was therefore concluded that chronic fluorosis is associated with decreased serum levels of these minerals.
Serum Sialic Acid Levels and Selected Mineral Status in Patients With Type 2 Diabetes Mellitus
(Humana Press inc, 2003) Ekin, S; Mert, N; Gunduz, H; Meral, I
The aim of the present study was to investigate whether altered serum total sialic acid (TSA), lipid-associated sialic acid (LSA), copper (Cu), manganese (Mn), zinc (Zn), chromium (Cr), iron (Fe), and magnesium (Mg) levels had an interactive connection with diabetes and also whether they were correlated with each other in diabetic patients. Two study groups (control and type 2 diabetic subjects) were included. Two hundred patients (108 female and 92 male), diagnosed and treated for type 2 diabetes in the Yuzuncu Yil University Hospital (Van, Turkey), were selected consecutively to represent type 2 diabetic patients. Fifty healthy individuals (29 female and 21 male) served as the control group matched for age, sex, body mass index, and smoking status were selected from hospital staff and other outpatient clinics. All participants had not taken vitamin or mineral supplements for at least 2 wk before sampling. Blood samples were drawn after an overnight fasting in both groups for the determination of serum glucose, TSA, LSA, Cu, Zn, Mn, Cr, Fe, and Mg. It was found that diabetics had higher TSA, LSA, Fe, Mn, Fe/Zn, and Cu/Zn levels, and lower Zn and Mg levels than those of controls. Although, Cu levels were higher, and Cr levels were lower in total and male diabetic patients, they were not different in female diabetic patients than in controls. The Cu/Fe ratio was lower in total and female diabetic patients, but not different in male diabetic patients than controls. The Zn/Cr ratio, on the other hand, was not different in diabetics than in controls. There was only a positive correlation between Fe-Mn levels in male diabetic patients. There was a negative correlation in LSA-Mn, Fe-Cu, Cu-Fe/Zn, and Mn-Cu/Zn levels in total diabetic patients. There was a positive correlation in TSA-Cr, TSA-Mg, LSA-Cu/Fe, LSA-Zn/Cr levels, and a negative correlation in TSA-Cu/Zn, LSA-Mn, Fe-Cu, Mn-Cu, Cu-Fe/Zn, Fe-cholesterol, and Cr-cholesterol in female diabetic patients. Our results showed that TSA, LSA, and selected minerals have interactive connections with diabetes mellitus (DM). There are also many sex-related positive or negative correlations between the altered parameters in diabetic patients. These parameters might be used as diagnostic index in patients with DM.
Serum Trace Elements Status of Rabbits Supplemented With Nigella Sativa, Vitamins C and E, and Selenium Against Damage by N-Methyl
(Humana Press inc, 2002) Gündüz, H; Dede, S; Agaoglu, ZT; Atasoy, N; Mert, N
In this study, we investigated the effects of Nigella sativa, vitamins C and E, and selenium on the levels of trace elements in the serum of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-injected rabbits. The rabbits were separated into one control and three experimental groups, each consisting of eight rabbits. MNNG was administered to all rabbits at a dose of 20 mg/kg. Group A received a suspension of N. sativa, group B received a combination of vitamins C and E and selenium, and group C received MNNG without any additional treatment. Group D did not receive any treatment and acted as control. The concentrations of serum zinc, copper, and iron were determined for groups A, B, C, and D. The zinc levels were 155.3 +/- 25.8, 304.7 +/- 14.22, 117.2 +/- 27.9, and 87.0 +/- 8 mu/dL for groups A-D, respectively; copper was measured at 234.8 +/- 31.9, 214.3 +/- 14.2, 196.5 +/- 19.3, and 359.2 +/- 19.9 mu/dL and iron levels were 276.3 +/- 10.71, 260.8 +/- 7.15, 211.2 +/- 13.47, and 223.4 +/- 9.5 mu/dL, in the stated group order. There were statistically significant differences between groups (p < 0.05).
Studies on the Effects of X-Ray on Erythrocyte Zinc and Copper Concentrations in Rabbits After Treatment With Antioxidants
(Humana Press inc, 2003) Dede, S; Deger, Y; Mert, N; Kahraman, T; Alkan, M; Keles, I
The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the erythrocyte concentrations of zinc and copper in rabbits after exposure to X-rays. The animals were divided into two experimental and one control group (CG). The first group (VG) was given daily oral doses of vitamins E and C; supplemental amounts of manganese, zinc, and copper were mixed with the feed and given to the second group of experimental animals (MG). Blood samples were taken from all groups before and after 4 wk of vitamin and mineral administration and after irradiation with a total dose of 550-rad X-rays. The administration of minerals caused the most significant increases of Zn and Cu. Even after irradiation, the zinc levels in the irradiated animals were higher than in the nonirradiated vitamin-supplemented animals (p<0.05). The results suggest that supplementation with antioxidant vitamins and minerals may have a protective effect against X-ray-induced damage.