Browsing by Author "Mert, Nihat"

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Advanced Oxidation Protein Product (Aopp) Levels in Second- and Third-Degree Thermal Burns
(Oxford Univ Press, 2021) Mert, Handan; Acikkol, Suat; Calli, Iskan; Cibuk, Salih; Keskin, Siddik; Mert, Nihat
Burn is a systemic injury affecting the entire organism according to its etiology and severity. The aim of this study was to investigate plasma AOPP levels before and after treatment of second- and third-degree thermal burn patients and determine the changes in this parameter, and also, to find out the relationship between AOPP level and hospitalization period and total body surface area (TBSA). The study material consisted of pediatric patients with the complaint of second- and third-degree thermal burns aged between 1 and 18 years, with a burn area exceeding 10%. Blood samples were taken twice before and after treatment. AOPP level in blood plasma was measured in ELISA. It was observed that in the second-degree thermal burn group, AOPP level was 25.85 +/- 2.82 ng/ml before the treatment decreased to 22.16 +/- 3.62 ng/ml after treatment, whereas in the third-degree thermal burn group before the treatment AOPP was 25.96 +/- 3.49 ng/ml, and after the treatment dropped to 21.70 +/- 3.79 ng/ml, decreases were significantly important (P < .05). There was no statistically significant difference between the two groups in terms of AOPP levels (P > .05). Correlation analyses in the second- and third-degree thermal burn group did not show any correlation between AOPP levels and burn area and length of hospitalization period. As a result, AOPP level has been studied, for the first time, in burn cases. In both groups, the level of AOPP increased due to oxidative stress before treatment and decreased after treatment.
Advanced Oxidation Protein Products (Aopp) Levels and Kidney Function in Fluorotic Sheep
(int Soc Fluoride Research, 2016) Mert, Handan; Comba, Bahat; Mert, Nihat; Cinar, Ali; Apaydin, Betul
The ingestion of excessive amounts of the fluoride ion (F) may cause chronic fluorosis with abnormalities in many organs and systems including, in addition to dental and skeletal fluorosis, impaired renal function. The aim of the present study was to investigate renal function and the levels of advanced oxidation protein products (AOPP) as oxidative stress markers in chronically fluorotic sheep with kidney disease. Fifteen healthy and 30 fluorotic sheep were studied. The plasma F levels were determined with a F-selective electrode. The plasma AOPP and the serum total protein levels were measured spectrophotometrically. The serum BUN, creatinine, potassium, sodium, and chloride levels were determined by autoanalyzer. Compared to the control group, significant increases were present in the fluorotic sheep in the levels of F (p=0.001), AOPP (p=0.001), BUN (p=0.001), creatinine (p= 0.05), and total protein (p= 0.05). A significant decrease was present in the fluorotic sheep in the level of potassium (p=0.01) while no significant changes were present in the sodium and chloride concentrations. The levels of AOPP in chronically fluorotic sheep, reported here for the first time in veterinary science, and the biochemical abnormalities can be considered in the evaluation of the effects of chronic fluorosis on kidney function.
An Investigation on the Hba1c, Mda, Gsh-Px and Sod Levels in Diabetic Rats
(2008) Çiftçi, Sevim; Mert, Nihat
İnsülin sekresyonu veya etkisindeki yetmezliği ile karakterize olan diabetes mellitus üzerinde yapılan bu çalışmada 7-8 haftalık Wistar cinsi sıçanlar kullanıldı. Rastgele olarak 10 sıçan kontrol, 20 sıçan ise hasta grup olarak ayrıldı. Deneysel diyabet oluşturmak için 30 mg/kg düzeyinde streptozotosin intraperiteonal uygulandı. Tüm hayvanlarda HbA1c, MDA, GSH-Px ve SOD düzeyleri usulüne uygun tekniklerle saptandı. HbA1c hasta grupta P<0.001 düzeyinde artış gösterirken, MDA düzeyinde saptanan yükselme istatiksel önem göstermemiştir (P>0.05). GSH-Px düzeyi hasta grupta azalmış (P<0.06), SOD miktarı ise hasta grupta artmıştır. Sonuç olarak, HbA1c'nin diabetes mellitus tanısında kullanılabilecek önemli bir parametre olduğuna ve diabetes mellitus'da antioksidant enzim değişimlerinin hastalığın prognozu açısından önemli olabileceği, klinisyenlerin diabetes mellitusta bu parametrelere dikkat etmelerinin yerinde olacağı sonucuna varıldı.
Antidiabetic and Antioxidant Effects of Pomegranate Oil in Stz Induced Diabetic Rats
(2020) Baskın, Rahaf Alhalabı; Mert, Nihat; Mis, Leyla
Diyabet 21. yüzyılın en büyük küresel sağlık sorunlarından biri olup en önemli 10 ölüm nedeninden biridir. Tedavi amacıyla kullanılan hipoglisemik ilaçlar ya çok pahalıdır ya da bazıları istenmeyen yan etkilere sahiptir. Daha etkili, güvenli ve daha düşük maliyetli antidiyabetik bitkiler aramak için araştırmalar yapılmaktadır. Bu çalışma, nar çekirdeği yağının kan şekeri, serum insülin düzeyleri ve bazı kan biyokimyasal parametreleri üzerine etkisini incelemek için yapıldı. 3 haftalık deneme için deneysel diyabetik sıçan modeli kullanıldı. Çalışmada Van Yüzüncü Yıl Üniveritesi Deney Hayvanları ünitesinden temin edilen 7-8 haftalık, canlı ağırlık ortalaması (185-250 g) olan 32 adet dişi Wistar cinsi Albino sıçan, her grupta 8 sıçan olacak şekilde rastgele seçilerek eşit olarak 4 gruba ayrıldı. Kontrol grubuna su verilirken, nar yağı grubuna gavaj yoluyla nar çekirdeği yağı (2 ml / kg / gün) verildi. Diyabet oluşturmak için üçüncü grup sıçanlara 45 mg/kg tek doz streptozotosin (STZ) i.p. yoldan uygulandı. Dördüncü grup sıçanlarda benzer şekilde STZ uygulanıp aynı dozda nar çekirdeği yağı gavaj yoluyla üç hafta boyunca her gün verildi. Deneme sonunda ratların kalplerinden kan örnekleri alınıp glukoz, insülin, adiponektin, resistin, toplam kolesterol (TC), trigliseritler (TG), HDL kolesterol, LDL-kolesterol, TAC, TOS, düzeyleri ölçüldü, OSI düzeyleri hesaplandı. Başlangıç ve son canlı ağırlıkları kaydedildi. Sunulan bu çalışmada TAC ve TOS seviyeleri kontrol ve nar çekirdeği grubunda değişmezken, diyabetin oksidan etkisi ile 3. grupta değişmiştir. TOS düzeylerinde gözlenen artış nar çekirdeği yağı verilmesiyle azalmıştır. Tersine olarak TAC miktarı diyabetli sıçanlarda azalırken nar çekirdeği yağı verilen grupta artış göstermiştir. Diyabetli sıçanlarda oksidan maddelerin varlığı ile yükselen OSI değerleri yine nar çekirdeği yağı ilavesi ile normal düzeye doğru azalmıştır. Son canlı ağırlıklar incelendiğinde diyabetli sıçanların en düşük değere sahip olduğu saptandı. Diyabetli sıçanlarda yükselen glukoz değeri nar yağı ilave edildiğinde önemli olarak düşmüştür. Adiponektin ve resisitin düzeylerinde önemli değişimler bulunmazken insülin düzeyi diyabetli grupta önemle azalmıştır. Trigliserit düzeyi tüm gruplarda benzer değerlerde saptandı. Diğer üç gruba göre diyabetli sıçanlarda LDL, kolesterol, TOS ve OSI düzeyleri artarken, HDL düzeyi azalmıştır. Bu bulgular nar çekirdeği yağının diyabette yükselen glukoz düzeyini düşürmesi, artmış kolesterol, LDL TOS ve OSI değerlerindeki yükselmeleri aşağıya çekmesi, ayrıca HDL ve TAC düzeylerindeki azalmalar üzerine yükseltici etkisi ile diyabette gözlenen olumsuz değişimleri düzeltebileceği, antioksidan olarak önemli bir madde olarak diyabette kullanılabileceği sonucuna varılmıştır. Anahtar kelimeler: Nar Çekirdeği Yağı, STZ, Diyabet, Antioksidan, Kolesterol, İnsülin, Resistin, Adiponektin.
Antidiabetic Effect of Fig Seed Oil in Rats With Diabetes Induced by Streptozotocin
(Japan Oil Chemists Soc, 2024) Mert, Handan; Mert, Nihan; Cibuk, Salih; Yildirim, Serkan; Mert, Nihat
The anti -diabetic effect of Ficus carica (Fig) seed oil was investigated. 4 groups with 6 rats in each group were used in the experiment as control, diabetes (45 mg/kg streptozotocin), fig seed oil (FSO) (6 mL/ kg/day/rat by gavage) and diabetes+FSO groups. Glucose, urea, creatinine, ALT, AST, GSH, AOPP and MDA analyses were done. Pancreatic tissues were examined histopathologically. When fig seed oil was given to the diabetic group, the blood glucose level decreased. In the diabetes+FSO group, serum urea, creatinine, AOPP, MDA levels and ALT and AST activities decreased statistically significantly compared to the diabetes group, while GSH levels increased significantly, histopathological, immunohistochemical, and immunofluorescent improvements were observed. It has been shown for the first time that FSO has positive effects on blood glucose level and pancreatic health. It can be said that the protective effect of fig seed oil on tissues may be due to its antioxidant activity.
Babesia Ovis ile Doğal Olarak Enfekte Koyunlarda Serum Folik Asit Düzeylerinin Araştırılması
(2008) Mert, Nihat; Dede, Semiha; Değer, Yeter; Mert, Handan; Yur, Fatmagül
Bu çalışma, Babesia ovis ile doğal olarak enfekte olan koyunlarda serum folik asit düzeylerinin araştırılması ve tedavi sonrasında nasıl etkilendiklerinin saptanması amacıyla yapıldı. Klinik ve parazitolojik olarak babesiosis tanısı konulan 30 adet koyun hasta grubunu, 10 adet sağlıklı koyun da kontrol grubunu oluşturdu. Hasta koyunlar, antiprotozoon ilaç (tek dozda diminazene aceturate’ın % 7 lik solüsyonu, i.m.) verilerek tedavi edildi. Tedavinin 7. gününde tekrar bu gruptan kan örnekleri toplandı. Yapılan analizlerde serum folik asit düzeylerinin hasta grupta en düşük olduğu (p<0.05), tedavi ile birlikte bu düzeylerin hastaya göre önemli oranda arttığı (p<0.05), ancak yine de kontrol grubuna göre önemli oranda azalmış olduğu tespit edildi. Sonuç olarak B. ovis enfeksiyonun serum folik asit düzeylerinin önemli oranda düşmesine yol açtığı, tedaviyle birlikte bir miktar arttığı görüldü.
Bisfenol-a’nın Sıçanlarda Oksidatif Durum ve Bazı İnflamatuar Belirteçler Üzerine Etkisi: Deneysel Çalışma
(2023) Çıbuk, Salıh; Yılmaz, Halil Cumhur; Mert, Handan; Mis, Leyla; Yoruk, Mecıt; Mert, Nihat
Amaç: Bisfenol-A [Bisphenol-A (BPA)], canlı dünyasında ve tüketici ürünlerinde yayg ın kullan ımı nedeniyle bir halk sa ğlığı sorunudur. Bu çalışmada, sindirilen BPA'nın sıçanlarda oksidatif durum ve baz ı inflamatuar belirteçler üzerindeki etkisinin ara ştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada, 24 adet Wistar Albino rat kullan ıldı ve 4 gruba (kontrol, sham, BPA25, BPA50) ayrıldı. Bu gruplardan birincisine herhangi bir uygulama yapılmazken, ikinci gruba (sham) m ısır ya ğı oral gavaj ı uygulandı. Deney gruplarından birincisine mısır yağında çözünmüş BPA 25 mg/kg/gün, ikincisine 50 mg/kg/gün dozlarda oral gavaj yoluyla verildi. Deneyin sonunda hayvanlara anestezi uyguland ı. Kalpten kan örnekleri al ındı ve serumlar ayrıldı. Toplam antioksidan kapasite, total oksidatif durum, tümör nekroz faktörü-alfa, interlökin (IL)-1 beta ve IL-6 düzeyleri enzim bağlı immünosorbent testi ile belirlendi. Bulgular: BPA 25-50 mg/kg/gün dozlarında uygulandığında, serum total antioksidan kapasite düzeyleri anlamlı derecede azalırken, toplam oksidatif durumda art ış görülmüş ve Oksidatif Stres İndeksi, BPA’n ın zararl ı etkilerini yansıtmaktadır. Ayrıca serum tümör nekroz faktörü-alfa, IL-1-beta ve IL-6 düzeyleri anlamlı olarak artmıştır (p≤0,05). Sonuç: Bu sonuçlar, BPA maruziyetinin inflamatuar belirteçleri indükledi ğini göstermektedir. BPA ayr ıca sadece oksidatif bile şiklerin üretimini teşvik ederek değil, aynı zamanda kontrol grubuna kıyasla antioksidan kapasiteyi azaltarak oksidatif strese neden olabilir. Tüm bu biyokimyasal değişiklikler, canlı organizmaların dokularındaki makro moleküllere zarar verebilir.
Changes in Heart Type Fatty Acid Binding Protein (h-Fabp) and Certain Biochemical Parameters During Chronic Artery Diseases
(2023) Ercan,; Mert, Nihat; Kaya, Yüksel; Günbatar, Nizamettin; Mert, Handan
In this study, changes in heart-type fatty acid binding protein (H-FABP) and certain biochemical parameters during chronic artery diseases were investigated. Coronary artery disease (CAD) is one of the leading causes of death and morbidity in our country,which is also true for the world in general as well. CAD generally develops on the foundations of atherosclerosis. In this study, blood samples collected from patients who applied to Van Yüzüncü Yıl University Dursun Odabaş Medical Center Emergency Department and Cardiology Polyclinics with acute ischemic chest pain between January 2019 and June 2019 were evaluated. Patients were not given any drugs or other kinds of substances before sample collection. Of the patients who applied to the cardiology clinic, 24 patients with acute ischemic chest pain were diagnosed with chronic arteritis, 12 patients with cardiological problems were diagnosed with diabetes mellitus (DM), 12 patients with hypertension (HT) problems and heart complaints, and 12 healthy individuals (who were not diagnosed with diabetes, hypertension or CAD) were included as research materials. Certain markers like Troponin, CK, CK-MB, AST, ALT, LDL-Cholesterol, HDL-Cholesterol, LDH, Glucose, and Creatinine in the blood samples were biochemically determined using an autoanalyzer (Abbott ci16200),while H-FABP values were determined using ELISA method. LDL-cholesterol levels were highest in the CAD group, HDL-Cholesterol and LDH levels were high in the CAD+HT group, while CK, CK-MB, cTnI, AST, Glucose, creatinine, and H-FABP levels were found to be high in the DM+CADgroup. Meanwhile, cTnI values were increased in people with HT or DM in addition to CAD, but there was no statistical significance. LDL-cholesterol changes also did not vary significantly between the groups. Intergroup changes in other parameters examined showed the importance of CK and Glucose levels at p≤ 0.001, AST p≤ 0.002, LDH p≤ 0.003, CK-MB p≤ 0.004, HDL-Cholesterol p≤ 0.049, Creatinine p≤ 0.011, and H-FABP p≤ 0.050. In recent years, H-FABP has taken its place in the field of cardiology with increasing importance in the diagnosis of CAD and MI. The findings obtained in this study show that the H-FABP level was increased in all patients examined (except the test group), and we recommend its use as a critical and useful parameter in the field of cardiology.
Concentration of Products of Nitric Oxide Oxidation and Some Vitamins in Sheep With Naturally Acquired Babesiosis
(Tubitak Scientific & Technological Research Council Turkey, 2009) Mert, Handan; Yoeruek, Ibrahim; Deger, Yeter; Mert, Nihat; Dede, Semiha; Yur, Fatmaguel
The aim of the present study was to determine serum concentrations of the products of nitric oxide oxidation (nitrate and nitrite) and some vitamins (retinol acetate, alpha- and delta-tocopherol, and vitamin D 3) in sheep naturally infected with Babesia ovis. The investigation included 30 infected and 10 control sheep. Serum a- tocopherol levels were significantly lower (P < 0.05), and nitrate and nitrite concentrations were significantly higher (P < 0.05) in infected animals than in controls. It is thought that the elevated nitrate and nitrite levels of the sheep infected with babesiosis were due to the result of damage caused by Babesia. On the other hand, a significant decrease was observed in a- tocopherol levels in sheep with babesiosis because of damage and pathophysiological changes to erythrocytes.
Determination of Vitamin D Level for the Past 5 Years of Women in the Region Between the Ages of 18-35 in Van Education and Research Hospital
(2018) Güneş, Ümit; Mert, Nihat; Akın, Mehmet Murat
Van Eğitim ve Araştırma Hastanesine Başvuran 18-35 Yaş Aralığındaki Bölge Kadınlarının Son 5 Yıllık D Vitamini Düzeyinin Saptanması. Van Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Biyokimya Anabilim Dalı, Veteriner Programı Yüksek Lisans Tezi, Van, 2018. Sunulan bu tez ile Van ilinde beş yıllık bir sürede çeşitli sağlık nedenleriyle Van Eğitim ve Araştırma hastanesine gelen bayan hastaların temelde Vitamin D düzeyleri olmak üzere onunla bağlantılı Ca, Mg ve K düzeylerini saptamak amaçlanmıştır. Çalışmanın insan materyalini 2012-2016 yılları arasında Van Eğitim ve Araştırma Hastanesine başvuran ve serum D vitamin analizleri yapılmış olan 18-35 yaş aralığındaki bayan hastalar oluşturdu. Her yıl için 20 hasta, toplam 100 hasta, gerekli izinleri alınıp araştırmaya dâhil edildi. 2012-2015 yıllları arasındaki istenilen analiz sonuçları arşivden alınırken, 2016 yılında ise yine 20 bayanın kan örnekleri bizzat alınıp, D vitamini, kalsiyum, potasyum ve magnezyum değerleri tayin edildi. Roche marka Cobas otoanalizörü kullanarak analizler yapıldı. 2012 ile 2016 yılında 5 yılık süre yılında bu parametrelerin ortalama değerleri sırasıyla Vitamin D için 7.88±8.23 – 11.90±10.88 - 10.67±10.39 – 12.68±13.76 – 15.98±15.72 ng/ml. Kalsiyum için 9.33±0.96 – 9.64±0.54 – 9.38±1.03 – 8.84±1.03 – 9.53±0.62 mg/dl, Magnezyum için 2.05±0.20 – 2.12±0.17 -2.03±0.14 – 1.93±0.22 – 2.00±0.26 mg/dl iken potasyum için 4.11±0.34 - 4.22±0.43 – 4.03±0.44 -4.10±0.47 – 4.18±0.36 mmol/l olarak saptandı. Çeşitli tıp otoriteleri tarafından 50 nmol /l (20-30 ng/ml) den fazla serum vitamin D düzeyi yeterli olarak kabul edildiğinden, klinik D vitamini eksikliğinde düzey 25 nmol/ l (10ng/ml)' den düşük olduğunda ortaya çıkmaktadır. İncelenen tüm yıllarda saptanan vitamin D düzeyleri eksiklik sınırları içinde olup Van ilinde kadınlarda ciddi eksiklikler bulunmuştur. Yöresel temelde kapalı olarak giyilen giysiler, gün ışığından faydalanma süresinin azlığı ve yetersiz beslenme eksiklik nedenleri olabilir. Diğer parametrelerde ciddi bir eksiklik tesbit edilmemiştir. Sonuç olarak Van ilinde yıllara dayanan bir çalışma olarak bu eser önem arzetmektedir. Daha detaylı Vitamin D seviye tesbiti çalışmalarının yapılması, bölgesel çalışmalara ağırlık verilerek, genç-yaşlı nüfüsta saptanacak eksik vitamin değerlerine karşı çeşitli kuruluşlar nezdinde, özellikle temel bazı gıdalar belirlenecek oranlarda takviye yapılması önerilmelidir. Anahtar Kelimeler: D Vitamini, Kadın, Kolekalsiferol, Kalsiyum, Magnezyum, Potasyum
Direct Sulfonamidation of (Hetero)aromatic C-H Bonds With Sulfonyl Azides: a Novel and Efficient Route Ton-(hetero)aryl Sulfonamides
(Royal Soc Chemistry, 2020) Liu, Zhi; Ebadi, Abdolghaffar; Toughani, Mohsen; Mert, Nihat; Vessally, Esmail
N-Aryl sulfonamides belong to a highly important class of organosulfur compounds which are found in a number of FDA-approved drugs such as dofetilide, dronedarone, ibutilide, sotalol, sulfadiazine, sulfamethizole, vemurafenib, and many more. There is therefore continuing interest in the development of novel and convenient protocols for the preparation of these pharmaceutically important compounds. Recently, direct sulfonamidation of (hetero)aromatic C-H bonds with easily available sulfonyl azides has emerged as an attractive and powerful strategy to accessN-(hetero)aryl sulfonamides where non-toxic nitrogen gas forms as the sole by-product. This review highlights recent advances and developments (2012-2020) in this fast growing research area with emphasis on the mechanistic features of the reactions.
The Effect of Cinnamaldehyde on The Activity of Glucose-6 Dehydrogenase, Some Biochemical and Hematological Parameters in Diabetic Rats
(Wiley, 2017) Celik, Remzi; Mert, Handan; Comba, Bahat; Mert, Nihat
The Effect of Emodin on Rats With Liver Damage by Carbon Techloride
(2024) Ağlamiş, Suzan; Mert, Nihat
Ağlamiş S. Karbon tetraklorür İle Karaciğer Hasarı Oluşturulan Ratlarda Emodin'in Etkisi Van Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Veteriner Biyokimya Anabilim Dalı, Yüksek Lisans Tezi, Van, 2024. Karbon tetraklorür(CCl4) canlılarda hepatotoksiteye neden olan bir ksenobiyotiktir. Serbest radikal üretimi ile hücresel hasar oluşturabilme özelliğine sahiptir ve toksisite modeli oluşturmak için yaygın olarak kullanılır. Emodin, Rhamnus cinsinin sayısız bitkisinin köklerinde ve kabuğunda bulunan doğal olarak oluşan bir antrakinondur . Emodinin serbest radikalleri inhibe etme, temizleme, enfeksiyona ve dejeneratif hastalıklara karşı koruma sağlamada önemli rolü vardır. Sunulan çalışmada, emodinin antioksidan ve anti-inflamatuar özellikleri ile CCl4'ü indüklediği hepatooksitidede koruyucu etkisi karaciğer dokusunda oluşabilecek histopatolojik değişimler araştırıldı. Çalışmada, 150-300 gr ağırlığında 32 adet dişi Wistar Albino rat kullanıldı. Hayvanlar rastgele olarak her birinde 8 sıçan olacak şekilde 4 gruba ayrıldı ve araştırma 8 gün sürdü. Kontrol grubuna Fizyolojik tuzlu su intraperiteonal (i.p) verildi. Emodin grubunda hayvanlara 10 mg/kg/gün dozunda emodin oral olarak verildi. CCl4 grubundaki sıçanlara sekizinci günde tek doz 1 mg/kg CCl4 verilirken CCl4+Emodin grubuna 10 mg/kg/gün dozunda emodin oral olarak verilip sekizinci günde tek doz 1 mg/kg CCl4 ip olarak birlikte verildi. Son CCl4 enjeksiyonundan 24 saat sonra (9. gün) tüm ratlara 90 mg/kg ketamin i.p uygulanıp kalpten kan örnekleri alındı. Serum örneklerinde ALT ve AST analizleri yapıldı. Karaciğer dokusunda ise histopatolojik inceleme ve biyokimyasal analizler yapıldı. Karaciğer dokusu mekanik olarak homojenize edilip ELISA kitleri kullanılarak GSH, MDA, AOPP, TNF-α, IL-6 analizleri yapıldı. CCl4 verilen grupta serum ALT ve AST düzeylerinde ciddi artışlar gözlenirken emodin verilmesiyle düzeylerde düşme gözlenmiş ve diğer gruplarla karşılaştırıldığında istatistiksel önem saptanmıştır. Karaciğer doku ekstraktı analizlerinde yine CCl4 grubunda GSH düzeyinde azalma saptanırken MDA, AOPP, TNF-α, IL-6 düzeylerinde artış gözlendi. Bu gruba emodin verilince GSH düzeyi yükselirken diğer parametrelerde önemli düşüşler saptandı. Karaciğer dokularının histopatolojik incelenmesinde, CCl4 grubunda asinar bölgede hepatositlerde şiddetli düzeyde dejenerasyon, nekroz ve damarlarda hiperemi gözlendi, emodin ilave edilen grubun karaciğer dokularında, asinar bölgede hafif düzeyde dejenerasyon ve nekroz olduğu belirlendi. Sonuç olarak, emodinin lipid peroksidasyonunu inhibe ederek, proinflamatuvar ajanları düzenleyip, antioksidan etkisiyle birlikte CCl4 kaynaklı hepatoksisiteye karşı önemli bir hepatoprotektif etkiye sahip olduğu gözlendi.
The Effect of Evening Primrose (Oenothera Biennis) on the Some Biochemical Parameters in Rats With Gentamicin Induced Nephrotoxicity
(2021) Mert, Nihat; Mert, Handan; İrak, Kıvanç; Medineli, Murat
In this study, it was aimed to investigate the effect of evening primrose oil (EPO) on somebiochemical parameters on nephrotoxicity induced by gentamicin (GM) in rats. The rats used in thestudy were randomly divided into 4 groups each consisting of 8 rats. The control group, EPO group,GM group and GM+ EPO group. The blood samples were taken 24 hours after the 8-day trial andkidneys were removed and saved for histopathological and PGE2 analysis. The serum creatinine,BUN, calcitriol, Ca, Na, Cl, K and P analyzes were performed via autoanalyser. PGE2 analysis wasperformed in kidney tissue via ELISA. Histopathological examination of the kidney tissues wasperformed. The levels of creatinine, BUN and Cl were significantly decreased and PGE2 and Caincreased in GM + EPO compared to GM group. The changes in the biochemical parametersexamined and the histopathological findings obtained, it can be said that the EPO weakens thenephrotoxic damage caused by GM and has the protective effects on the kidney.
The Effect of Evening Primrose Oil (Oenothera Biennis) on the Level of Adiponectin and Some Biochemical Parameters in Rats With Fructose Induced Metabolic Syndrome
(Taylor & Francis Ltd, 2022) Mert, Handan; Irak, Kivanc; Cibuk, Salih; Yildirim, Serkan; Mert, Nihat
The effect of evening primrose oil on adiponectin level and some biochemical parameters in model of fructose-induced metabolic syndrome were investigated. The rats were divided into 4 groups: control, evening primrose oil, fructose, fructose + evening primrose oil. Body weight, daily feed and water consumptions and systolic blood pressures of animals were measured. At the end of trial, blood samples were taken, livers were excised and histopathological examination was performed. Glucose, uric acid, triglyceride, T.cholesterol, LDL, HDL, VLDL, ALT, AST, ALP, LDH, adiponectin, insulin, IL-6, TNF-alpha, TAC, and TOS levels were analysed. Some analysed parameters and systolic blood pressure of fructose + evening primrose oil group decreased significantly compared to fructose group and adiponectin, TAC, and HDL levels were significantly increased. As conclusion, evening primrose oil can be considered as antioxidant agent by reducing oxidative stress, increasing adiponectin levels and insulin sensitivity, anti-inflammatory properties, exhibiting anti-atherogenic effect by regulating dyslipidemia and systolic blood pressure.
The Effect of Evening Primrose Oil on Some Biochemical Parameters in Brain Tissue in a Model of Metabolic Syndrome Induced With Fructose in Rats
(2023) Günbatar, Nizamettin; Mert, Handan; Çıbuk, Salıh; Mis, Leyla; Mert, Nihat
Objective: Metabolic syndrome is a disease characterized by hypertension, dyslipidemia and insulin resistance, and constitutes an important risk factor for cardiovascular disorders. The effect of evening primrose oil (EPO) on insulin, adiponectin and resistin levels in brain tissue was investigated in a fructose- related metabolic syndrome model. Materials and Methods: The rats were divided into 4 groups as control, evening primrose oil (orally at a dose of 0.1 mLrat/day), fructose (20% fructose added), fructose+evening primrose oil for 57 days. At the end of the experiment, brain samples were taken and homogenized. Then, insulin, adiponectin and resistin in brain tissue levels were determined by ELISA. Results: Plasma insulin and resistin levels of the fructose group increased (p≤0.05) compared to the controls, on the contrary, adiponectin levels were significantly decreased (p≤0.05) in the fructose group. When EPO was given to rats given fructose, increased insulin and resistin levels decreased (2.54± 0.28a, 2.12±0.68a), (2.21±0.26b, 2.04±0.21a) while decreased adiponectin levels were increased (0.64±0.42c, 1.02±0.35b). Conclusion: It was observed that the impaired metabolic changes caused by fructose in the brain tissue were partially improved in the EPO-treated group as a result of the decrease in insulin, resistin and increase in adiponectin. Accordingly, since metabolic changes in the brains of rats fed with high fructose content may also occur in humans with fructose intake from various foods, the use of EPO in the medical setting may be recommended by clinicians to reduce the harmful effects on the brain.
The Effect of Fucoidan on Changes of Some Biochemical Parameters in Nephrotoxicity Induced by Gentamicin in Rats
(Ankara Univ Press, 2018) Ataman, Nese; Mert, Handan; Yildirim, Serkan; Mert, Nihat
Fucoidan is a polysaccharide with high viscosity and mucilage, which contains significant proportion of sulphate ester group and L-fucose. It is present in intercellular spaces of brown algae. This study aimed to investigate the effect of fucoidan on some biochemical parameters and kidney tissues in nephrotoxicity induced by GM in rats. The rats used in the study were randomly divided into 4 groups. Each group had 7 rats as control, fucoidan, GM and GM+fucoidan. Blood samples were taken after 24 hours from the end of experiment which lasted eight days. Creatinine, BUN, uric acid, glucose, triglycerides, total cholesterol, VLDL, HDL, total bilirubin levels and ALT, AST, ALP, LDH, CK, amylase activities were assayed by an autoanalyser. The kidney tissues were examined histopathologically. In GM+fucoidan group, creatinine (p<0.001), BUN (p<0.001), uric acid (p<0.05), triglycerides (p<0.05), VLDL (p<0.05), AST (p<0.001), ALP (p<0.05), LDH (p<0.001), CK (p<0.01) levels statistically decreased, however HDL (p<0.05) level increased when compared to GM group. As conclusion, it can be said that administering fucoidan during GM toxicity decreases the nephrotoxicity damage. Considering biochemical parameters and histopathological findings, fucoidan can be suggested as a protective agent for kidneys in nephrotoxicity induced rats.
The Effect of Fucoidan on the Changes of Some Biochemical Parameters and Protein Electrophoresis in Hepatotoxicity Induced by Carbontetrachloride in Rats
(Agricultural Research Communication Centre, 2018) Aysin, Nesrullah; Mert, Handan; Mert, Nihat; Irak, Kivanc
Fucoidan is a sulfate polysaccharide extracted from brown algae. Fucoidan has various pharmacological properties, such as anti-tumor, anti-mutagenic, anti-inflammatory, antiviral, antioxidant, anti-fibrogenic activity and anti-complementary activities Objective: This study was aimed to explore the effect of fucoidan on biochemical parameters (ALT, AST, GGT, total protein, albumin, globulin) and protein fractions in hepatotoxicity induced by CCl4 in rats. Materials and Methods: The rats used in the study were randomly divided into four groups of 8 rats each: Control group, fucoidan group, fucoidan+CCl4 group and CCl4 group. After 24 hours from the process of an eight-day experiment, blood samples were taken. The analysis of ALT, AST, GGT activities and total protein, albumin, globulin levels were done by an autoanalyser and serum protein fractions (albumin, alpha 1- globulin, alpha 2-globulin beta- globulin, gamma- globulin and A/G ratio) were electrophoretically determined. Results: In the group of fucoidan+CCl4, it was determined that the levels of AST (p<0.001), GGT (p<0.001), total protein (p<0.01), globulin (p<0.01), beta-globulin % (p<0.01), gamma-globulin % (p<0.001) statistically decreased compared to CCl4 group. Conclusion: It can be said that fucoidan has the property of hepatoprotectant by looking to some biochemical parameters and changes in protein fractions that examined in hepatotoxicity induced by CCl4.
The Effect of Fucoidan on The Gentamicin Induced Nephrotoxicity in Rats
(Parlar Scientific Publications (p S P), 2018) Yilmaz, Mesut; Mert, Handan; Irak, Kivanc; Erten, Remzi; Mert, Nihat
Possible preventive or therapuetic effects of fuciodan, which has antioxidant properties and was administered together with gentamicin (GM) made up nephrotoxicity through giving GM to the rats, have been searched. The rats used in this study were randomly grouped in three: control, GM (80 mg/kg/day/i.p GM was administered for 8 days), GM+fucoidan (80 mg/kg/day/i.p GM for 8 days + fucoidan 100 mg/kg/day/ for 8 days were administered with intra-gastric gavage) groups. Blood and tissue samples were taken 24 hours after the 8 days trial period. Besides determining plasma creatinine, BUN, sodium, potassium, chloride levels in auto-analyser, whole blood glutathione, plasma MDA and AOPP levels were analyzed spectrophotometrically. The highest plasma creatinine, BUN, potassium, AOPP and MDA levels were found in the GM group. It was determined that while the levels of creatinin (p<0.01), BUN (p<0.05), potassium (p<0.05), AOPP (p<0.01), MDA (p<0.01) in GM+fucoidan group were decreasing with regard to those in GM group, chloride levels were rising (p<0.05). The study revealed the nephroprotective effect of fucoidan. These findings suggest that fucoidan administration together with GM may attenuate renal dysfunction and structural damage through the reduction of oxidative stress in the kidney.
The Effect of Pepper Gas (Oc) on Some Biochemical Parameters in Rats
(Kafkas Univ, veteriner Fakultesi dergisi, 2012) Seyhan, Ercan; Mert, Nihat; Mert, Handan
In this dissertation, the effect of the widely used pepper gas (oleoresin capsicum, OC) derived from the cayenne pepper was studied in the blood gases, electrolytes and some biochemical parameters on the rats with different dosages and periods. In the pre-dosage phase, 12 of 47 Wistar albino rats were used as the live material. Commercially available 90 g OC sprays were provided. It was experimentally measured that 6 g of OC was sprayed per second. 6 rats were used,as control group. Experimental Group 1 was 9 rats which were kept 5 min in an OC sprayed closed enviroment for 4 sec (24 g); Experimental Group 2 was 10 rats which were kept 8 min in an OC sprayed closed enviroment for 8 sec (48 g); Experimental Group 3 was 10 rats which were kept 15 min in an OC sprayed closed enviroment for 12 sec (72 g). The rats were kept as a group of 5 of each in 50x50x50 cm experiment cube and OC was applied for the abovementioned periods and dosages. The blood samples were taken from the heart after the 1-2 min sedation application. The potential hydrogen (pH), carbon dioxide partial pressure (pCO(2)), concentration of total carbon dioxide (ct CO2), O-2% saturation, HCO3, base excess (BE), base excess of extracellular fluid (B(ecf)), Na, K, Ca, Cl and glucose levels were measured by Rapidlab 865 System and auto analyser. When the control group values were compared to the other three groups, pH, ct CO2 and HCO3 (except the Group 3) were decreased, pCO(2), pO(2), O-2% saturation, K, Cl, glucose, BE and B(ecf) were increased and no significant change was observed in Na and Ca levels. The pH change (P <= 0.05), pO(2) (P <= 0.01), HCO3 (P <= 0.01); %O-2 saturation (P <= 0.001), K (P <= 0.05), Cl (P <= 0.05); B(ecf) (P <= 0.05) levels were evaulated the changes according to inter-group and control group. As a result, no fatality were encountered in rats which were exposed different dosages and amounts of OC in a closed enviroment except difficulty in breathing and conjuctivity. Despite that supporting biochemical changes for respiratoric asidose were encountered, it was understood that pepper gas could especially be used as a safe riot control agent in open air.