Browsing by Author "Meydan, Ismet"

Now showing 1 - 20 of 22

Antioxidant, Anti-Lipid Peroxidation and Antimicrobial Effect of Heracleum Persicum
(2024) Okumus, Emine; Meydan, Ismet
This study was conducted to determine the antioxidant activity, lipid peroxidation inhibitory effect and antimicrobial properties of Heracleum persicum plant. DPPH (2, 2-diphenyl, 1-picrylhydrazyl) method was used to determine antioxidant activity, and the Folin method was used to determine total phenolic content. Disc diffusion method was applied to determine antimicrobial activity. In the study, the antioxidant activity value of H. persicum was determined as 5.36 mg mL-1, the total phenolic content was 20.84 mg GAE mL-1 and the total flavonoid content was 12.35 mg QE mL-1. The plant's inhibition against lipid peroxidation was measured as 3.63 mg mL-1. Its antioxidant activity and inhibitory effect against lipid peroxidation were lower than butylated hydroxyanisole (BHA) in both analyses (p<0.05). The plant extract was found to be effective against Bacillus subtilis, Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus bacteria. As a result, the antioxidant properties, antimicrobial and anti-lipid peroxidation effects of H. persicum plant have revealed that this plant has an important potential for use in medicine and pharmacology, as well as for consumption as food.
Assessment of Therapeutic Potential of Silver Nanoparticles Synthesized by Ferula Pseudalliacea Rech. F. Plant
(Elsevier, 2022) Kocak, Yilmaz; Oto, Gokhan; Meydan, Ismet; Seckin, Hamdullah; Gur, Tugba; Aygun, Aysenur; Sen, Fatih
Plant-mediated synthesis of silver nanoparticles (Ag NPs) has increased its use in various biomedical applications due to its cost-effectiveness, renewable and environmentally friendly properties. Therefore, the focus of the study was to synthesize Ag NPs and to evaluate their antibacterial, and antioxidant activities by using the aqueous root extract of Ferula pseudodalliacea plant as a reducing agent. In addition, the synthesis of nanoparticles was confirmed by performing Ag NPs/Fp characterization processes. According to the findings of our research, color change due to surface plasmon resonance was confirmed by UV-vis spectrometry. The crystal properties of Ag NPs/Fp were determined according to the XRD model. Phytochemicals responsible for coating and reduction of Ag NPs/Fp were observed by FT-IR analysis. It has been shown that the therapeutic effect of Ag NPs/Fp exhibits better antimicrobial and antioxidant activity than aqueous extract. In addition, it was determined that Ag NPs/Fp structures showed the best antifungal effect against Candida albicans ATTC 90028 pathogen and gave a better zone than Rifampicin antibiotic. Therefore, studies at the molecular level and more comprehensively are required to determine the bioactive components and pharmacological effects of Ferula pseudodalliacea plant to confirm our antibacterial, antioxidant, and antifungal activity results.
Characterization of Rheum Ribes With Zno Nanoparticle and Its Antidiabetic, Antibacterial, Dna Damage Prevention and Lipid Peroxidation Prevention Activity of in Vitro
(Academic Press inc Elsevier Science, 2022) Meydan, Ismet; Burhan, Hakan; Gur, Tugba; Seckin, Hamdullah; Tanhaei, Bahareh; Sen, Fatih
This study aims to investigate the antidiabetic, antimicrobial, DNA damage, and lipid peroxidation prevention activity of ZnO NPs/Rr formed as a result of the interaction of Rheum ribes (R.ribes) plant with ZnO. The ZnO NPs/Rr obtained as a result of the reaction were confirmed using high-reliability characterization methods. According to the data obtained as a result of the study, it is seen that the activity of ZnO NPs/Rr to prevent lipid peroxidation is quite strong. Lipid peroxidation inhibition activity of ZnO NPs/Rr at the highest concentration of 250 mu g/ml was calculated as % 89.1028. It was observed that ZnO NPs/Rr prevented DNA damage by % 92.1240 at the highest concentration of 100 mu g/ml. It was determined that the antidiabetic effect of ZnO NPs/Rr formed by ZnO of R. ribes plant, which is used as a medicinal plant as an antidiabetic, was significant. It appears to have a strong antidiabetic property compared to the positive control acarbose. In our current study, it was observed that ZnO NPs/Rr formed zones ranging from 8 +/- 3.0 to 21 +/- 4.5 against Gram-positive and Gram-negative microorganisms. It has been determined that ZnO nanoparticles have an antibacterial effect.
Chitosan/Pva-supported Silver Nanoparticles for Azo Dyes Removal: Fabrication, Characterization, and Assessment of Antioxidant Activity
(Royal Soc Chemistry, 2024) Meydan, Ismet; Aygun, Aysenur; Tiri, Rima Nour Elhouda; Gur, Tugba; Kocak, Yilmaz; Seckin, Hamdullah; Sen, Fatih
With the advancement of technology, studies in the field of nanotechnology have attracted great interest in recent years. The fact that nanomaterials have superior advantages over micromaterials provides a wide range of uses. Green synthesis is an effective way to prepare nanomaterials with an easy, fast, and environmentally friendly method. Within the scope of the study, AgNPs were synthesized using basil extract and combined with chitosan/PVA as a support material. By using chitosan/PVA support materials, the surface area of AgNPs was increased and it was aimed to improve their properties. The synthesized AgNPs@chitosan/PVA nanocomposite was characterized using various methods. In the UV-Vis spectrum, an absorbance peak was observed at 430 nm for the AgNPs@chitosan/PVA nanocomposite, and the particle size was determined as 25.10 nm according to TEM results. In addition, the photocatalytic and antioxidant activities of AgNPs@chitosan/PVA nanocomposite were investigated. The antioxidant activity of the AgNPs@chitosan/PVA (100 mu g mL-1) nanocomposite against DPPH and H2O2 was determined as 89.18% and 71.87%, respectively. The photocatalytic activity of the AgNPs@chitosan/PVA nanocomposite against methylene blue (MB), methylene red (MR), methylene orange (MO), safranin, and crystal violet (CV) dyes was 77%, 85%, 79%, 54%, and 9%, respectively. While the highest photocatalytic activity was observed against MR dye, very low photocatalytic activity was observed for CV. In light of the results obtained, it can be said that the AgNPs@chitosan/PVA nanocomposite has the potential to be used as an antioxidant agent and photocatalyst. The photocatalytic activity of AgNPs@chitosan/PVA nanocomposite was tested against different dyes such as methylene blue, methylene orange, and methylene red as an effective photocatalyst.
Effects of X-Ray Application on Infertility in New-Born Rats
(Taylor & Francis Ltd, 2023) Cibuk, Salih; Mert, Handan; Mert, Nihat; Tuncer, Oguz; Altindag, Fikret; Karaman, Kamuran; Meydan, Ismet
In this study, the effect of early X-ray exposure on infertility was investigated by creating a newborn model with rats. Fifteen Pregnant rats were divided into five groups. After birth, the study was continued with 12 babies (6 males, 6 females) rat in each group. Different amounts of X-rays were applied to the experimental groups. At the end of the experiment, there was found that testosterone levels decreased in all experimental groups compared to the control group (P < 0.05). When the experimental groups were compared to the control group, there was a decrease in the number of spermatogoniums from all the experimental groups. The decrease in group II, group III and group IV was found to be statistically significant (P < 0.05). As a result, exposure to X-rays in new-borns and premature babies; It was observed that it caused disruption of caspase signaling in gonad organs, a serious decrease in hormonal activity, a significant decrease in spermatogonia number and a decrease in the number of primordial follicles. Considering these results, it can be predicted that exposure to X-rays in the neonatal period, especially in the premature period, may lead to infertility in later life.
Efficient Green Photocatalyst of Silver-Based Palladium Nanoparticles for Methyle Orange Photodegradation, Investigation of Lipid Peroxidation Inhibition, Antimicrobial, and Antioxidant Activity
(Pergamon-elsevier Science Ltd, 2022) Karimi, Fatemeh; Rezaei-savadkouhi, Negin; Aygun, Aysenur; Tiri, Rima Nour Elhouda; Meydan, Ismet; Aghapour, Elaheh; Ucar, Mustafa
Nanotechnology is an interdisciplinary study that has been developing worldwide in recent years and has a serious impact on human life. The fact that the nanoparticles of plant origin are clean, non-toxic, and biocom-patible has enabled new fields of study. The Hibiscus sabdariffa (H. sabdariffa) plant has been attracted by sci-entists because of its impact on health and many other areas. The lipid peroxidation inhibiting activity, antioxidant properties, and antimicrobial properties of H. sabdariffa plant with Ag-Pd metal was ditermined. For the total phenolic component, gallic acid was used as the standard and quarcetin was used for the total flavonoid. The lipid peroxidation inhibition activity of Ag-Pd NPs in ethanol extract was found to be very well compared to the positive control (BHA). The lowest and highest concentrations of DPPH radical scavenging activity were 82.178-97.357%, whereas for BHA these values were found to be 84.142-94.142%. The highest concentration of Ag-Pd NPs at 200 mu g/mL the DPPH radical quenching activity was higher than BHA. Ag-Pd NPs showed a good antimicrobial activity against certain pathogenic microorganisms such as Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, which are the causative agents of various diseases in humans. The photodegradation activity of Ag-Pd NPs also investigated against Methyl orange dye (MO) under sunlight irradiation for 120 min and was found to be as 67.88%
Electrochemical Characterization of Vitamin B2 (Riboflavin) at Ag Nanoparticles Synthesized by Green Chemistry Dispersed on Reduced Graphene Oxide
(Elsevier Science Sa, 2023) Guler, Muhammet; Meydan, Ismet; Seckin, Hamdullah
The present study describes a novel electrochemical riboflavin (RF) sensor based on the green synthesis of silver (Ag) nanoparticles dispersed on reduced graphene oxide (rGO). The Ag nanoparticles was synthesized using Arum italicum leaf extract as reducing and stabilizing agent. The characterization of Ag nanoparticles was carried out using FT-IR, XRD, and SEM-EDX. The electrochemical studies were performed using cyclic voltammetry and differential pulse voltammetry. The Ag/rGO modified glassy carbon electrode (Ag/rGO/GCE) exhibited excellent electrochemical responses to the oxidation/reduction (redox) of RF. The sensor exhibited a linear determination range from 2 x 10-9 to 2.2 x 10-6 M with limit of detection (LOD) of 0.6 nM. The sensor had the sensitivity of 71.4 mu A mu M-1 cm-2. The sensor had satisfactory selectivity, reproducibility of 3.4 % and storage stability of 90.4 %. Also, the fabricated sensor was proved to be useful for the determination of RF in pharmaceutical samples.
An Environmental Approach for the Photodegradation of Toxic Pollutants From Wastewater Using Pt-Pd Nanoparticles: Antioxidant, Antibacterial and Lipid Peroxidation Inhibition Applications
(Academic Press inc Elsevier Science, 2022) Seckin, Hamdullah; Tiri, Rima Nour Elhouda; Meydan, Ismet; Aygun, Aysenur; Gunduz, Meliha Koldemir; Sen, Fatih
Background: Green synthesis is an effective and friendly method for the environment, especially in recent years has been used in many areas. It finds application opportunities in many fields such as physics, chemistry, electronics, food, and especially health and is the subject of intensive studies in this field. Objectives: The synthesized Pt-Pd NPs were aimed to be used as a bio-based photocatalyst under sunlight to prevent wastewater pollution. In addition, it is aimed to use Pt-Pd NPs as biological agents in different applications in the future. Methods: In this study, the platinum-palladium nanoparticles were synthesized by the extract of Hibiscus sabdariffa, the characterization of the nanoparticles was carried out by different methods (ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), infrared transform spectroscopy atomic force microscopy (AFM), and ray diffraction (XRD) analysis). And we discussed several different parameters related to human health by obtaining platinum-palladium bimetallic nanoparticles (Pt-Pd NPs) with a green synthesis method. These parameters are antioxidant properties (total phenolic, flavonoid, and DPPH scavenging activity), antibacterial activity, and lipid peroxidation inhibition activity. Gallic acid was used as standard phenolic, and quercetin was used as standard flavonoid reagents. The newly synthesized Hibiscus sabdariffa mediated green synthesized Pt-Pd NPs were compared with gram-positive and gram-negative bacteria, the high antibacterial activity was shown by gram-positive bacteria. The photodegradation of Pt-Pd NPs was carried out against MB dye for 180 min. Results: TEM results show that the average size of Pt-Pd NPs is around 4.40 nm. The total amount of phenolic compounds contained in 0.2 mg/ml of Pt-Pd NPs was equivalent to 14.962 +/- 7.890 mu g/ml gallic acid and the total amount of flavonoid component was found to be equal to 28.9986 +/- 0.204 mu g/ml quercetin. Hibiscus sabdariffa mediated green synthesized Pt-Pd NPs was found to have very effective for lipid pemxidation inhibition activity in the FeCl2-H2O2 system. The maximum DPPH scavenging activity was determined as 97.35% at 200 mu g/mi. The photocatalytic activity of Pt-Pd NPs was analysed against Methylene blue (MB) and the maximum degradation percentage was observed to be 83.46% at 180 min. Conclusions: The biogenic Pt-Pd NPs showed a high effective photocatalytic and biological activity.
Erica Arborea Bitkisi Kullanılarak Sentezlenen Çinko Nanopartiküllerin Biyoaktivitesinin Değerlendirilmesi
(2023) Koçak, Yılmaz; Meydan, Ismet
Nanopartiküller (NP’ler), çevre dostu, uygun maliyetli ve basit yöntemlerle sentezi, tıp ve endüstri alanlarında pek çok araştırmaya konu olmuştur. NP’ler özellikle yeni nesil nano tabanlı ilaçların geliştirilmesi için umut verici bir yaklaşım sunmaktadır. Bu çalışmada, Erica arborea (E. arborea) yaprak özütü stabilize edici ve indirgeyici ajan olarak kullanıldı ve çinko oksit NP’ler (ZnO NP’ler) sentezlendi. Hazırlanan ZnO NP’ler ultraviyole-görünür spektroskopisi (UV-vis), fourier dönüşümlü kızılötesi spektroskopisi (FTIR), taramalı elektron mikroskopisi (SEM) and enerji dağılımlı X-ışını spektroskopisi (EDX) analizleri ile karakterize edildi. SEM ve EDX analizlerinde, NP’lerin küresel şekilde olduğu ve çinko metalinin güçlü sinyalleri görüldü. UV-vis analizi, çözeltinin renginin açık sarıya dönüşüyle NP'lerin oluşumunu doğruladı ve 350 nm'de bir absorpsiyon piki sergiledi. FT-IR, NP'lerin oluşumuna yaprak ekstresinin metabolitlerinin eşlik ettiğini doğruladı. E. arborea tarafından sentezlenen ZnO NP'lerin biyolojik aktivitesi antimikrobiyal, antioksidan ve lipid peroksidasyon analizleri ile gerçekleştirildi. Biyojenik ZnO NP’ler, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis ve Staphylococcus aureus patojen bakterileri ve Candida albicans mantarına karşı duyarlı olduğu bulundu. Özellikle Bacillus subtilis ve Candida albicans rifampin antibiyotiğine göre daha etkiliydi. ZnO NP’ler, bitki ekstraktına kıyasla daha iyi antioksidan aktivite sergiledi. ZnO NP'ler ve yaprak ekstraktının DPPH radikal süpürme aktivitesinin IC50 değerleri sırasıyla 9.21±0.10 ve 18.71±0.35 idi. Ayrıca ZnO NP'lerin lipid peroksidasyon inhibitör aktivitesi için IC50 değeri 5.33±0.09 olarak bulunmuştur. Bulgular, doğal olarak sentezlenen çinko NP’lerin sentezlenmesi, fiziksel ve kimyasal yöntemlere alternatif bir ajan olabileceğini göstermektedir. Ayrıca NP’lerin biyolojik aktivitesi yeni nesil ilaç geliştirme çalışmalarına katkı sağlayacaktır.
Evaluation of Protective Effects of Gallic Acid on Cisplatin-Induced Testicular and Epididymal Damage
(Wiley, 2021) Altindag, Fikret; Meydan, Ismet
Cisplatin is an effective chemotherapeutic drug used to treat many types of tumours. However, it may cause male reproductive toxicity. Gallic acid exhibits beneficial effects such as antioxidant, anti-inflammatory and antitumor. The current study investigated the beneficial effects of gallic acid against testis and epididymis toxicity induced by cisplatin. Male rats were divided into 4 groups as follows (n = 7): Control, cisplatin (a single dose of 8 mg/kg), Gallic acid (50 mg/kg) and cisplatin +Gallic acid groups. Testis was examined morphometrically by stereological methods. In addition, apoptosis, DNA damage, oxidative stress parameters in testis and testosterone in serum were measured. Epididymis was histopathologically evaluated. As a result, a significant decrease was observed in the number of spermatogonia, Leydig and Sertoli cells, testicular volume, height of germinal epithelial, Bcl-2 immunopositive cell number, activity of CAT, GSH and SOD enzymes and serum testosterone levels compared with the cisplatin group control group, while a significant increase was observed in the number of Caspase-3, Bax and 8-OHdG immunopositive cells and the MDA levels. However, Gallic acid significantly restored these parameters. Our study reveals that Gallic acid may improve Cisplatin-induced male reproductive toxicity by reducing oxidative stress, suppressing apoptosis and DNA damage and restoring structural and functional deterioration.
Green Synthesis, Characterization and Bioactivity of Biogenic Zinc Oxide Nanoparticles
(Academic Press inc Elsevier Science, 2022) Gur, Tugba; Meydan, Ismet; Seckin, Hamdullah; Bekmezci, Muhammed; Sen, Fatih
In this study, we tried to enlighten the structure of zinc oxide nanoparticles (ZnO NPs) obtained from Thymbra Spicata L. plant by using green synthesis method in various ways. Some properties of zinc oxide nanoparticles were determined by using the characterization methods that scanning electron microscopy (SEM), Energy Dispersive X-ray analysis (EDX), fouirer transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet visible spectroscopy (UV-Vis) spectroscopy methods. The detected Zn nanoparticle sizes were determined to be between 6.5 nm and 7.5 nm. In addition to these studies, we investigated the antimicrobial effects of zinc oxide nanoparticles obtained by green synthesis against some pathogens. According to the results, it was seen that zinc oxide nanoparticles formed zones with a diameter of 16.3 mm, 10.25 mm, 13 mm and 10.2 mm, respectively, against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, Pseudomonas aeruginosa ATCC 27853 bacteria and Candida albicans ATTC 90028 fungus, respectively. However, the radical quenching activity (DPPH) of the nanoparticles (Ts-ZnONP (79.67%)) was determined to be quite good compared to the positive control BHA. In addition, it is seen that the protective effect of ZnO NPs against DNA damage increases depending on the concentration. At a concentration of 100 mg/L, the DNA damage inhibitory effect was found to be maximum. In line with the comprehensive results, it was determined that the zinc oxide nanoparticles obtained with the green synthesis method have the potential of use in a wide variety of fields.
Green Synthesis, Characterization, Antimicrobial and Antioxidant Activities of Zinc Oxide Nanoparticles Using Helichrysum Arenarium Extract
(2021) Meydan, Ismet; Seçkin, Hamdullah
Active substance production at the nano-level attracts attention in the scientific world due to its wide applicationarea. Different methods are used for the biosynthesis of nanoparticles. Recent studies have focused on non-toxic,environmentally friendly synthesis methods. Nanoparticles obtained by green synthesis using various biologicalelements such as plants, microorganisms and proteins have taken part in many scientific studies. Plants, whichhave an important potential in active ingredient production, are highly preferred in nanoparticle production.Scanning Electron Microscope and Energy Dispersive X-Ray Analysis (SEM / SEM-EDX), Fourier TransformInfrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD) and Ultraviolet visible light absorption Spectroscopy(UV-vis) techniques were used for the structural and morphological characterization of Zn nanoparticles obtainedby green synthesis using Helichrysum arenarium plant extract and ZnO.The antioxidant capacity of Zn NPs/Ha structures was determined by performing the DPPH test. Antimicrobial effects of zinc nanoparticles onsix different pathogens (Bacillus cereus ATCC 10876, Escherichia coli ATCC 25952, Bacillus subtilis ATCC6633, Staphylococcus aureus ATTC 29213, Pseudomonas aeruginosa ATCC 27853 and Candida albicansATTC 90028) were investigated. As a result of this studies, it has been observed that it has an inhibitory effectagainst some pathogen microorganisms. It has also been found that its antioxidant content is at a significant level.
In Vitro Dna Damage, Protein Oxidation Protective Activity and Antioxidant Potentials of Almond Fruit (Amygdalus Trichamygdalus) Parts (Hull and Drupe) Using Soxhlet Ethanol Extraction
(Springer, 2020) Meydan, Ismet; Kizil, Goksel; Demir, Halit; Ceken Toptanci, Bircan; Kizil, Murat
Almond is a nut that is consumed abundantly all over the world. In Turkey, almonds are grown in most parts of the Turkey and the consumption of nuts has become an essential part of healthy life. Almond contain powerful nutrients, which may be related to their fatty acid composition. Therefore, the aim of present study was to investigate the antioxidant potentials and the protective activity of ethanol extraction ofAmygdalus trichamygdalusfruit parts (Hull and Drupe) against the lipid peroxidation, oxidative DNA damage and protein oxidation using soxhlet ethanol extraction. To the best of our knowledge, our study can be evaluated as the first report. The total phenolic content was found to be equivalent to 28.16 +/- 9.990 mu g/g gallic acid extract and the total flavonoid content was equivalent to 8.866 +/- 0.208 mu g/g quercetin extract. The present study shows thatA. trichamygdalusextract have good protective activity against lipid peroxidation in the FeCl2-H(2)O(2)system. The extract also showed a strong DPPH radical scavenging ability in a concentration-dependent manner. At 2000 mu g/ml, the DPPH radical scavenging activity was found to be 84.47 +/- 0.147%. The protein oxidation was also inhibited byA. trichamygdalusextract. At 2000 mu g/ml,A. trichamygdalusextract protected protein oxidation by 76.27 +/- 1.14%. In addition, the DNA oxidation damage against OH radicals resulting from H(2)O(2)photolysis was inhibited concentration dependently and DNA damage was suppressed by 50.26 +/- 2.6% at 1000 mu g/ml concentration. The results suggest thatA. trichamygdalusfruit extract have antioxidant activity and protect lipid, protein and DNA against oxidative damage. Therefore, the extract may be useful in the food industry as natural antioxidants.
Interleukin-8 (il-8) Levels in Gingival Crevicular Fluid During Root Canal Treatment of Molar Teeth With Symptomatic Irreversible Pulpitis: Impact of Varying Sodium Hypochlorite Concentrations
(Bmc, 2024) Ozlek, Esin; Kadi, Gizem; Shoshaa, Nasser; Saed, Yusuf; Meydan, Ismet
Background The aim of this study was to evaluate the effect of the use of different NaOCl concentrations (1%, 2.5%, and 5.25%) during root canal treatment of molar teeth with symptomatic irreversible pulpitis on the change of the IL-8 level in gingival crevicular fluid (GFC). Methods GCF sampling was performed on experimental tooth with irreversible pulpitis before and after treatment and also contralateral healthy tooth of 54 patients. The patients were divided into three groups according to concentration of NaOCl solution (n = 18); 1%, 2.5%, and 5.25% NaOCl solution. GCF sampling from experimental teeth was repeated one week after root canal treatment. Statistical analysis was performed using Mann-Whitney U, Wilcoxon test, one-way ANOVA and Pearson correlation analysis. Results There was a significant correlation between IL-8 levels in GCF samples taken from teeth with pulpitis before treatment and from healthy contralateral teeth (p = .000). Furthermore, the pretreatment IL-8 level was significantly higher than the posttreatment IL-8 level(p < .05). The effect of the NaOCl concentration on the change in IL-8 level betweeen pre and post treatment was not found statistically significant (p > .05). Conclusions The use of NaOCl during root canal treatment can effectively reduce the levels of IL-8 in GCF and improve clinical outcomes.
Metagenomic Analysis of Intestinal Microbiota in Florated Rats
(Springernature, 2022) Komuroglu, Ahmet Ufuk; Seckin, Hamdullah; Ertas, Metin; Meydan, Ismet
Changes in gut microbiota have shown that it plays an important role in animal health and metabolic diseases. The intestinal microbiota is a complex structure that functions as an organ system with the presence of trillions of microorganisms. In this study, changes in the intestinal microbiota of Wistar rats with high fluorine were evaluated. Water containing 100 ppm NaF was given to 14 male Wistar albino rats as drinking water for 12 weeks. Fluorine is known to be an inducer of protein oxidation, lipid peroxidation, modulation of intracellular redox homeostasis, and oxidative stress. In this study, it was determined that the level of MDA (molandialdehyde), one of the oxidative stress parameters, increased significantly in the intestinal tissue after fluorine intoxication. The decrease in CAT (catalase) and SOD (superoxide dismutase) enzyme activities was found to be statistically significant. Intestinal tissues were taken under aseptic conditions and microorganisms found in flora were replicated by V3-V4 16S rRNA gene-specific primers. As a result of the sequence analysis, a statistical comparison of the control group and the fluorine applied group was made. The study we have done showed that there was a significant difference in species diversity in the intestinal microbiota of mice treated with fluorine. As a result, the composition of the intestinal microflora, especially Lactobacillus species, was significantly changed in rats with high fluorine.
Microwave-Assisted Fabrication of Agruni Trimetallic Nps With Their Antibacterial Vs Photocatalytic Efficiency for Remediation of Persistent Organic Pollutants
(Springer, 2024) Kocak, Yilmaz; Tiri, Rima Nour Elhouda; Aygun, Aysenur; Meydan, Ismet; Bennini, Nihed; Karahan, Tugba; Sen, Fatih
Antibiotic-resistant bacteria have long been a concern and become a public health problem around the world. Therefore, various methods are being tried to develop new-generation therapeutic agents against antibiotic resistance. One of these methods is biogenic nanoparticle (NP) synthesis. Besides the antibacterial properties of biogenic NPs, it also contributes to the solution of the environmental toxic waste problem. In this study, we present a more environmentally friendly microwave approach to produce AgRuNi trimetallic nanoparticles (AgRuNi TNPs). The structural properties and chemical composition of TNPs were characterized by several techniques such as XRD, TEM, FTIR etc. The average particle size of AgRuNi TNPs was found to be 3.61 +/- 0.63 nm. For the removal of a persistent organic pollutant, such as methyl orange (MO) under solar irradiation, the photocatalytic activity of AgRuNi TNPs to remove MO dye was examined. The photodegradation percentage (%) of AgRuNi TNPs against MO dye after 300 min was calculated as 92%. Additionally, the antibacterial efficacy of the generated nanoparticles against gram (+) and gram (-) was evaluated. Overall, the current method to produce AgRuNi TNPs is based on the principles of green chemistry and pharmaceutical technology and shows promise for the creation of highly effective photocatalytic systems and the development of drug delivery systems to generate antibacterial activity.
Production of Plant-Based Zno Nps by Green Synthesis; Anticancer Activities and Photodegradation of Methylene Red Dye Under Sunlight
(Springer Heidelberg, 2024) Demir, Canan; Aygun, Aysenur; Gunduz, Meliha Koldemir; Altinok, Bahar Yilmaz; Karahan, Tugba; Meydan, Ismet; Sen, Fatih
Discharging wastewater from factories into streams without cleaning them causes environmental pollution and threatens the health of living beings. The photocatalysis method developed for easy, fast, and low-cost wastewater cleaning attracts considerable attention and therefore needs to synthesize efficient photocatalysts. In addition, the synthesized non-toxic catalysts can be tested as anticancer agents and may be suitable candidates to solve the issues facing cancer therapy today. Therefore, the synthesis of biogenic nanomaterials has gained importance. Plant-mediated green synthesis stands out in the simple, fast, cheap, and non-toxic production of nanoparticles (NPs). NPs produced by green synthesis are frequently used in biological applications such as anticancer and photocatalyst applications for cleaning wastewater. In this article, zinc oxide nanoparticles (ZnO NPs) were obtained using Zingiber officinale extract. The plant extract functions as a reducing, capping, and stabilizing agent in the green production of ZnO NPs. The synthesis of ZnO NPs was characterized using UV-Vis spectroscopy, FTIR, XRD, and TEM. TEM results show that ZnO NPs have sizes in the range of 10-45 nm. The anticancer activity of ZnO NPs was examined against HT29 (ATCC (R) HTB-38 (TM)) colorectal cancer cell line and HepG2 (ATCC (R) HB-8065 (TM)) hepatocellular cancer cell line by MTT assay. The IC50 values of ZnO NPs against HT29 and HepG2 cancer cell lines after 48 h were calculated as 20 mu g/mL and 45 mu g/mL, respectively. Additionally, the photocatalytic activity of ZnO NPs was tested against methylene red azo dye under sunlight and 87.96% (pH 7) photodegradation was obtained at the end of the 120th minute. It is predicted that ZnO NPs can be used effectively in both anticancer studies and environmental improvement.
Protective Effect of Silymarin and Gallic Acid Against Cisplatin-Induced Nephrotoxicity and Hepatotoxicity
(Wiley-hindawi, 2022) Dogan, Duygu; Meydan, Ismet; Komuroglu, Ahmet Ufuk
Objective. This study aimed to investigate the effects of gallic acid and silymarin against nephrotoxicity and hepatotoxicity caused by cisplatin. Materials and Methods. In the study, 56 Wistar Albino rats were equally divided into eight groups. Group 1 was the control group; group 2 was the group receiving cisplatin; group 3 was the group receiving cisplatin + gallic acid; group 4 was the group receiving cisplatin + silymarin; group 5 was the group receiving cisplatin + silymarin + gallic acid; group 6 was the group receiving silymarin; group 7 was the group receiving gallic acid; group 8 was the group receiving gallic acid + silymarin. AST, ALT, urea, creatinine, albumin, globulin, and total protein levels were measured at the end of the study. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), glutathione (GSH), and 8-hydroxy-2 & PRIME;-deoxyguanosine (8OH-dG) levels were measured in kidney and liver tissues. Additionally, histopathological evaluations of the tissues were also performed. Results. In kidney and liver tissues, cisplatin significantly increased MDA and 8-OHdG levels compared with treatment groups (p < 0.05). Silymarin-treated group significantly increased the SOD activity and GSH amount in the liver tissue compared with the cisplatin-treated group (p < 0.05). Gallic acid significantly increased CAT activity compared with the cisplatin-treated group (p < 0.05). It was determined that the cisplatin-treated group significantly decreased CAT and SOD activity compared with the control group (p > 0.05). Gallic acid showed a significant increase in CAT and SOD activity in kidney tissue compared with the cisplatin-treated group (p < 0.05). Conclusion. As a result, it was observed that gallic acid silymarin had a protective effect on cisplatin-induced nephrotoxic and hepatotoxic effects.
Research of Some Trace Elements and Heavy Metal Levels at the Premature, Pre-Term and Term Births
(Parlar Scientific Publications (p S P), 2021) Turan, Kasim; Uckan, Kazim; Meydan, Ismet; Demir, Halit; Demir, Canan
The purpose of the study is to research some trace elements and heavy metal levels at the premature, preterm and term births. There are many genetic and environmental etiologic factors causing to the prematurity. This study was undertaken to understand how the unknown etiology is associated with heavy metal and trace element values in pre-term, preterm, and premature-preterm conditions. Measurement of the Zn, Cd, Fe, Mg, Mn, Pb, Co and Cu elements was determined with Atomic Absorption Spectrophotometer method. Fe, Co, Cu, Mg, Mn ve Zn levels of the premature preterm, preterm and term patients was found to be lower compared with the healthy control group (p<0.05). Pb and Cd levels were found higher at the premature preterm, preterm and term patients compared with the healthy control group (p<0.05). Some trace element and heavy metal levels at the premature preterm, preterm and term births may play an important role at the etiopathogenesy of the disease. This study is first and original at the literature.
Selective Extraction and Determination of Citrinin in Rye Samples by a Molecularly Imprinted Polymer (Mip) Using Reversible Addition Fragmentation Chain Transfer Precipitation Polymerization (Raftpp) With High-Performance Liquid Chromatography (Hplc) Detection
(Taylor & Francis inc, 2021) Meydan, Ismet; Bilici, Mustafa; Turan, Eylem; Zengin, Adem
Citrinin (CIT) is a mycotoxin naturally in many foods that causes carcinogenic and mutagenic effects in the human body. A novel method was developed for the selective quantification of citrinin in rye samples. Citrinin-imprinted spheres were fabricated through reversible addition fragmentation chain transfer (RAFT) precipitation polymerization (RAFTPP) in the presence of 2-hydroxymethacrylate (HEMA, functional monomer), ethylene glycol dimethacrylate (EGDMA, cross-linker), citrinin (template), 4-cyano-4-(phenylcarbonothioylthio)pentanoic acid (CTA, RAFT agent), azobisisobutyronitrile (AIBN, initiator), and acetonitrile (ACN, porogen). The imprinted polymers were shown to be spherical with a high surface area and a porous structure. The rebinding properties of citrinin to the imprinted spheres were also examined in detail. The maximum adsorption capacity, equilibration time, and imprinting factor were 38.6 mg g(-1), 90 min, and 3.89, respectively. In addition, the citrinin-imprinted spheres were regenerated at least 10 times without change in the adsorption capacity. The citrinin-imprinted particles were used to selectively remove and determine the analyte in rye extract. The calibration relationship was linear between 1 and 100 mu g kg(-1) with a limit of detection of 0.35 mu g kg(-1). The method also had high recoveries (98 to 100.0%) and low relative standard deviations (less than 4.1%). Therefore, the imprinted spheres are suitable for the selective determination of citrinin in rye extracts.