Browsing by Author "Mis, Leyla"

Now showing 1 - 20 of 35

Anaplasma Phagocytophilum in Horses - Evaluation of Proinflammatory Biomarkers
(Univ Fed Rio Grande Do Sul, 2022) Mis, Leyla; Oguz, Bekir
Background: Anaplasma phagocytophilum is an obligate intracellular pathogen transmitted by the ticks that cause equine granulocytic anaplasmosis (EGA). This pathogen is infects predominantly blood cells, principally granulocytes and especially neutrophils. A. phagocytophilum causes an acute febrile disease in horses accompanying with lethargy, loss of appetite, lameness and hemorrhages. In horses, this disease should be considered in all acute symptoms accompanied by thrombocytopenia and leukopenia identified by hematological test performed. Tick-borne pathogens have become increasingly threatening for both animals and also public health since ticks mostly carry numerous well-documented and undocumented pathogens, and the geographical range of ticks has expanded in the recent years. This research has aimed to evaluate the impact of A. phagocytophilum infection on some oxidative/nitrosative stress parameters, antioxidant enzyme activities, proinflammatory biomarkers and trace element levels in horses. Materials, Methods & Results: The present study has been carried out using blood samples collected from 93 horses aged 1-year and older. The blood samples were centrifuged and sera were separated. Serum samples stored in the freezer (-20 degrees C) until the day of analysis. The DNA was extracted from blood and analysed by nested-PCR technique targeting 16S rRNA gene of A. phagocytophilum and then positive PCR products were sequenced. A. phagocytophilum was 6 horses (6.4%) showed positive nested-PCR results. An infected group comprised of 6 positive horses according to PCR analysis results also 6 healthy horses as control were selected. Serum SOD (Horse Superoxide Dismutase(Cu-Zn)) ELISA Kit, MPO (ELISA Assay Kit Horse Myeloperoxidase) and GPx (Horse glutathione peroxidase 1 ELISA Kit Assay), IL1 (Horse Interleukin 1 Beta ELISA Kit), IL6 (Horse Interleukin 6 ELISA Kit), TNF alpha (Horse Tumor Necrosis Factor Alpha ELISA Kit) and IL18 (Horse Interleukin 18 (IL18) ELISA Kit) levels were determined by ELISA reader. Serum TAS ,TOS Glucose-6-phosphate dehydrogenase (G6PD) levels were determined using colorimetric kit method. The determination of peroxynitrite was performed using spectrophotometry as described by Vanuffelen. The levels of Fe, Zn, Se, Cu, Mn, Ar, Cr, Co, Cd Ni and Pb elements were analyzed by ICP-OES. Total oxidant status (TOS) and peroxynitrite levels of the positive group infected with A. phagocytophilum were found to be higher compared with the control group (P < 0.05, respectively). Whereas, total antioxidant status (TAS), glutathione peroxidase (GPx) ,superoxide dismutase (SOD), G6PD and myeloperoxidase (MPO) levels were detected to be statistically significantly higher than the control group (P < 0.05, respectively). Cu, Mn, Se and Zn levels were also found to be lower in the infected group (P < 0.05, respectively). IL1, IL6, TNF alpha and IL18 from proinflammatory markers were elevated in the A. phagocytophilum infected group (P < 0.05, respectively). Discussion: The evaluation of the proinflammatory biomarkers with respect to host-parasite interactions has been suggested as a beneficial clinical tool for determination of the infection severity and diagnosis of the disease. As a conclusion, increased oxidative stress and high levels of some proinflammatory biomarkers assessed by the multibiomarker analysis carried out for the infection in the horses caused by Anaplasma phagocytophilum will provide a contribution to diagnosis, treatment and clarification of the pathogenesis.
Antidiabetic and Antioxidant Effects of Pomegranate Oil in Stz Induced Diabetic Rats
(2020) Baskın, Rahaf Alhalabı; Mert, Nihat; Mis, Leyla
Diyabet 21. yüzyılın en büyük küresel sağlık sorunlarından biri olup en önemli 10 ölüm nedeninden biridir. Tedavi amacıyla kullanılan hipoglisemik ilaçlar ya çok pahalıdır ya da bazıları istenmeyen yan etkilere sahiptir. Daha etkili, güvenli ve daha düşük maliyetli antidiyabetik bitkiler aramak için araştırmalar yapılmaktadır. Bu çalışma, nar çekirdeği yağının kan şekeri, serum insülin düzeyleri ve bazı kan biyokimyasal parametreleri üzerine etkisini incelemek için yapıldı. 3 haftalık deneme için deneysel diyabetik sıçan modeli kullanıldı. Çalışmada Van Yüzüncü Yıl Üniveritesi Deney Hayvanları ünitesinden temin edilen 7-8 haftalık, canlı ağırlık ortalaması (185-250 g) olan 32 adet dişi Wistar cinsi Albino sıçan, her grupta 8 sıçan olacak şekilde rastgele seçilerek eşit olarak 4 gruba ayrıldı. Kontrol grubuna su verilirken, nar yağı grubuna gavaj yoluyla nar çekirdeği yağı (2 ml / kg / gün) verildi. Diyabet oluşturmak için üçüncü grup sıçanlara 45 mg/kg tek doz streptozotosin (STZ) i.p. yoldan uygulandı. Dördüncü grup sıçanlarda benzer şekilde STZ uygulanıp aynı dozda nar çekirdeği yağı gavaj yoluyla üç hafta boyunca her gün verildi. Deneme sonunda ratların kalplerinden kan örnekleri alınıp glukoz, insülin, adiponektin, resistin, toplam kolesterol (TC), trigliseritler (TG), HDL kolesterol, LDL-kolesterol, TAC, TOS, düzeyleri ölçüldü, OSI düzeyleri hesaplandı. Başlangıç ve son canlı ağırlıkları kaydedildi. Sunulan bu çalışmada TAC ve TOS seviyeleri kontrol ve nar çekirdeği grubunda değişmezken, diyabetin oksidan etkisi ile 3. grupta değişmiştir. TOS düzeylerinde gözlenen artış nar çekirdeği yağı verilmesiyle azalmıştır. Tersine olarak TAC miktarı diyabetli sıçanlarda azalırken nar çekirdeği yağı verilen grupta artış göstermiştir. Diyabetli sıçanlarda oksidan maddelerin varlığı ile yükselen OSI değerleri yine nar çekirdeği yağı ilavesi ile normal düzeye doğru azalmıştır. Son canlı ağırlıklar incelendiğinde diyabetli sıçanların en düşük değere sahip olduğu saptandı. Diyabetli sıçanlarda yükselen glukoz değeri nar yağı ilave edildiğinde önemli olarak düşmüştür. Adiponektin ve resisitin düzeylerinde önemli değişimler bulunmazken insülin düzeyi diyabetli grupta önemle azalmıştır. Trigliserit düzeyi tüm gruplarda benzer değerlerde saptandı. Diğer üç gruba göre diyabetli sıçanlarda LDL, kolesterol, TOS ve OSI düzeyleri artarken, HDL düzeyi azalmıştır. Bu bulgular nar çekirdeği yağının diyabette yükselen glukoz düzeyini düşürmesi, artmış kolesterol, LDL TOS ve OSI değerlerindeki yükselmeleri aşağıya çekmesi, ayrıca HDL ve TAC düzeylerindeki azalmalar üzerine yükseltici etkisi ile diyabette gözlenen olumsuz değişimleri düzeltebileceği, antioksidan olarak önemli bir madde olarak diyabette kullanılabileceği sonucuna varılmıştır. Anahtar kelimeler: Nar Çekirdeği Yağı, STZ, Diyabet, Antioksidan, Kolesterol, İnsülin, Resistin, Adiponektin.
Arpa Çiminin Diyabetli Ratlarda Antioksidan Kapasitesine ve Dna Hasarına Etkisi
(2023) Başbuğan, Yıldıray; Comba, Bahat; Aksu, Devrim Sarıpınar; Comba, Arzu; Mis, Leyla
Amaç: Dünya çapında fitoterapi yöntemleri büyük önem kazanmakta ve bu alandaki çalışmalar her geçen gün önemini artırmaktadır. Bu çalışmada deneysel olarak diyabet oluşturulan ratlarda toplam antioksidan, oksidan durum, oksidatif DNA hasarı, glikoz ve hemoglobin A1c düzeyleri ve arpa çimi suyunun bu parametreler üzerine etkisinin incelenmesi amaçlandı. Gereç ve yöntem: Çalışma için 4 grup oluşturuldu ve her grupta 250-350 gr ağırlığında 6 erkek rat kullanıldı. Kontrol grubu; serum fizyolojik periton içi yol ile enjekte edildi, Diyabet grubu; diyabet, streptozotosin kullanılarak oluşturuldu, Arpa çimi grubu; 4 hafta oral olarak arpa çimi suyu (3 ml/sıçan/gün) verildi, Diyabet+Arpa çimi grubu; streptozotosin ile diyabet oluşturuldu ve 4 hafta boyunca arpa çimi suyu oral olarak verildi. Bulgular: Arpa çimi suyu, streptozotosin kaynaklı diyabette kan şekeri, glikoz, HemoglobinA1c, toplam oksidatif durum ve oksidatif stres gösterge değerlerini düşürdüğü, toplam antioksidan değerini ve canlı ağırlıkları arttırdığı belirlendi. Ayrıca arpa çimi suyu ilavesinin bu parametreler üzerinde belirgin koruyucu etki ve iyileşme sağladığı tespit edildi. Sonuç: Bu bulgulardan yola çıkarak arpa çimi suyunun şeker hastalığında anti-diyabetik, anti-oksidan etki gösterdiği ve kilo kaybını önlediğini söyleyebiliriz.
Assessment of the Wheatgrass Effects on Antioxidant Capacity and Dna Damage in Rats With Experimental Renal Failure
(Parlar Scientific Publications (p S P), 2019) Mis, Leyla; Comba, Bahat; Basbugan, Yildiray; Yasar, Semih; Comba, Arzu
In this study, it was aimed to evaluate the effect of wheatgrass on antioxidant capacity and DNA damage in rats with renal failure. In the study, 200-300 g 24 healthy Wistar-Albino male rats obtained from YYU Experimental Animal Unit were used. The rats were randomly divided into 4 groups and each group was formed with 6 rats. Groups were allocated as; 1. Control Group, 2. Renal Failure Group (Genta Group) 3. Renal Failure + Wheatgrass Group (Genta +Wheatgrass) 4. Wheatgrass Group. Gentamicin sulfate was given intraperitoneal at a dose of 50 mg/kg for 1 week and once a day to the groups with renal failure (Groups 2 and 3). Wheat grass extract (10 ml/kg) was added to the drinking water of the wheatgrass group rats (3rd and 4th groups) once a day. This practice lasted for 4 weeks. Total oxidant, antioxidant quantities, DNA damage were evaluated at the end of 4 weeks. The result that wheatgrass could be useful in renal failure was reached.
Between 2010 and 2011, Factors Affecting Incidence of Tuberculosis in Van
(2013) Karasungur, Remzi; Mis, Leyla
Karasungur R, Van İlinde 2010-2011 Yılı Tüberküloz İnsidansını Etkileyen Faktörler. YYÜ Sağlık Bilimleri Enstitüsü Fizyoloji Anabilim Dalı Yüksek Lisans Tezi, Van, 2013. Bu çalışmada, Van'da Tbc insidansını ve buna etki eden faktörleri incelemek ve yapılacak iyileştirmeler için fikir oluşturmak amaçlanmıştır. Verem savaş dispanserlerinin Tbc bildiriminde kullandıkları standart aylık formlar 2010-2011 yılları arasında retrospektif olarak incelendi. Bu dönemde 267 Tbc hastası saptandı. 2010-2011 yıllarının ortalama Tbc insidansı 12.8'dir.Tüberküloz hastalarının çoğunluğunu (%43)'ü 15-34 yaş grubunun oluşturduğu belirlendi. Tbc insidansı 2010 yılında yüksek bulundu. Bunda, şehrin kötü sosyoekonomik faktörlerinin rol oynadığı düşünüldü. 2011 yılında ise tüberküloz insidansında düşüş görüldü. Tüm bu verilerin tüberküloz ile savaşta yapılacak yeni düzenlemelere yardımcı olabileceğini düşünmekteyiz. Doğrudan gözetim altında tedavi stratejisi ile tedavi süreci mutlaka takip edilmelidir. Anahtar Kelimeler: Tüberküloz, insidans, sosyoekonomik faktörler.
Bisfenol-a’nın Sıçanlarda Oksidatif Durum ve Bazı İnflamatuar Belirteçler Üzerine Etkisi: Deneysel Çalışma
(2023) Çıbuk, Salıh; Yılmaz, Halil Cumhur; Mert, Handan; Mis, Leyla; Yoruk, Mecıt; Mert, Nihat
Amaç: Bisfenol-A [Bisphenol-A (BPA)], canlı dünyasında ve tüketici ürünlerinde yayg ın kullan ımı nedeniyle bir halk sa ğlığı sorunudur. Bu çalışmada, sindirilen BPA'nın sıçanlarda oksidatif durum ve baz ı inflamatuar belirteçler üzerindeki etkisinin ara ştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada, 24 adet Wistar Albino rat kullan ıldı ve 4 gruba (kontrol, sham, BPA25, BPA50) ayrıldı. Bu gruplardan birincisine herhangi bir uygulama yapılmazken, ikinci gruba (sham) m ısır ya ğı oral gavaj ı uygulandı. Deney gruplarından birincisine mısır yağında çözünmüş BPA 25 mg/kg/gün, ikincisine 50 mg/kg/gün dozlarda oral gavaj yoluyla verildi. Deneyin sonunda hayvanlara anestezi uyguland ı. Kalpten kan örnekleri al ındı ve serumlar ayrıldı. Toplam antioksidan kapasite, total oksidatif durum, tümör nekroz faktörü-alfa, interlökin (IL)-1 beta ve IL-6 düzeyleri enzim bağlı immünosorbent testi ile belirlendi. Bulgular: BPA 25-50 mg/kg/gün dozlarında uygulandığında, serum total antioksidan kapasite düzeyleri anlamlı derecede azalırken, toplam oksidatif durumda art ış görülmüş ve Oksidatif Stres İndeksi, BPA’n ın zararl ı etkilerini yansıtmaktadır. Ayrıca serum tümör nekroz faktörü-alfa, IL-1-beta ve IL-6 düzeyleri anlamlı olarak artmıştır (p≤0,05). Sonuç: Bu sonuçlar, BPA maruziyetinin inflamatuar belirteçleri indükledi ğini göstermektedir. BPA ayr ıca sadece oksidatif bile şiklerin üretimini teşvik ederek değil, aynı zamanda kontrol grubuna kıyasla antioksidan kapasiteyi azaltarak oksidatif strese neden olabilir. Tüm bu biyokimyasal değişiklikler, canlı organizmaların dokularındaki makro moleküllere zarar verebilir.
The Changes in the Levels of Elements in Sheep With Contagious Ecthyma
(Agricultural Research Communication Centre, 2018) Yeltekin, Asli Cilingir; Karapinar, Zeynep; Mis, Leyla
This study has aimed to investigate the differences between the levels of trace elements in sheep with the diagnosis of ecthyma, and in healthy sheep. For this purpose, Be, Bi, Pb, Cd, Na, K, Ca, Mg, Fe, Cu, Zn, Se, Ni and Mn, Co, Cr, Li elements in the plasma of sheep with Contagious ecthyma detected with PCR and in sheep in the control group were analyzed with ICP-OES. In this study, the levels of Fe, Cu, Zn, Se and Pb trace elements were found to be statistically different between the sheep infected with parapoxvirus. The Na ele ment of the macro elements was observed to be statistically different in sheep with ecthyma, to the control group(P<0.05). When there are low levels of metal concentration in sheep with ecthyma, their immune system can be considered to be weakened. Further studies can be carried out on the increase in the risk of developing the disease as a result of, in particular, a decrease in the Fe, Cu, Zn and Se concentrations in sheep infected with the virus.
Dynamics of Oxidants, Antioxidants and Hormones During Different Phases of Pregnancy in Hairy Goats
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Cetin, Nebi; Eski, Funda; Mis, Leyla; Naseer, Zahid; Bolacali, Memis
The aim of the present study was to observe the variation in oxidant (MDA), antioxidants (SOD, GSH, GSH-Px) and hormones (P4 and E2) levels in pregnant hairy goats during breeding season. In the this study, twenty hairy goats were synchronized by using sponges containing progesterone (fluorogestone acetate). The animals showing oestrus were inseminated twice, first at 18th -24th h and second at 36th - 48th h of oestrus. On 35th and 42nd days after insemination, pregnancy diagnosis examination was performed with transrectal ultrasonography. The blood samples were collected from pregnant goats at 0,11, 24, 57, 100, 134, 141 days of gestation and immediately after the parturation. Serum samples collected at estrus and different stages of gestation were analyzed for MDA, SOD, GSH-Px, GSH, P4 and E2 concentrations using standard protocols. The results showed that MDA level did not change in pregnant goats. The SOD, GSH and GSH-Px levels also shown a similar pattern throughout pregnancy period. The peak (P<0.05) level of progesterone was recorded between 11th to 134th days of gestation. At time of estrus (day 0) and late gestation (day 134 and 141), the concentrations estradiol reached at maximal level (P<0.05) in hairy goats. In conclusion, the oxidants and antoixidants do not change with respect to dynamics of progesterone or estradiol level during gestation period in black hairy goats.
The Effect of Barley Grass on Antioxidant Capacity and Dna Damage in Rat With Renal Failure
(Parlar Scientific Publications (p S P), 2017) Comba, Bahat; Mis, Leyla; Uslu, Sema; Comba, Arzu
This study was aimed to search the effect of barley grass (BG) on the Total Antioxidant (TAS)-Oxidant Status (TOS) and DNA damage (8OHdG) in rat with renal failure. The rats which are used in the study were randomly divided into 4 groups that each of has 8 rats: Control (C) group; injected i.p. with physiological saline once a day for 7 days, GM group; Gentamicin (80 mg/kg/day) was injected i.p. for 7 days, BG group; was given oral BG (250 mg/kg/day) for 4 weeks, GM+BG group was injected gentamicin (80 mg/kg/day) with i.p. for 7 days and BG (250 mg/kg/day) was given by oral for 4 weeks. After from the process of experiment for 4 weeks, blood sample and kidney tissue were taken. The analyses of urea and creatinine were done by autoanalyser; TAS, TOS levels by colorimetric kits; 8OHdG level by ELISA kits in serum. The kidney tissues were examined histologically. In the GM+BG group was statistically decreased levels of urea, creatinine, TOS (p<0.05) and OSI (p<0.01) compared to GM group. In the group of BG was determined the levels of TAS p<0.05 statistically increased other groups. The statistical significance was not found in the level of serum 8OHdG differences between the groups. BG decreased widespread damage to the renal glomerulus and proximal tubulus. As a result, adverse effects of high dose gentamicin application such as kidney damage can be reduced when used barley grass.
The Effect of Evening Primrose Oil on Some Biochemical Parameters in Brain Tissue in a Model of Metabolic Syndrome Induced With Fructose in Rats
(2023) Günbatar, Nizamettin; Mert, Handan; Çıbuk, Salıh; Mis, Leyla; Mert, Nihat
Objective: Metabolic syndrome is a disease characterized by hypertension, dyslipidemia and insulin resistance, and constitutes an important risk factor for cardiovascular disorders. The effect of evening primrose oil (EPO) on insulin, adiponectin and resistin levels in brain tissue was investigated in a fructose- related metabolic syndrome model. Materials and Methods: The rats were divided into 4 groups as control, evening primrose oil (orally at a dose of 0.1 mLrat/day), fructose (20% fructose added), fructose+evening primrose oil for 57 days. At the end of the experiment, brain samples were taken and homogenized. Then, insulin, adiponectin and resistin in brain tissue levels were determined by ELISA. Results: Plasma insulin and resistin levels of the fructose group increased (p≤0.05) compared to the controls, on the contrary, adiponectin levels were significantly decreased (p≤0.05) in the fructose group. When EPO was given to rats given fructose, increased insulin and resistin levels decreased (2.54± 0.28a, 2.12±0.68a), (2.21±0.26b, 2.04±0.21a) while decreased adiponectin levels were increased (0.64±0.42c, 1.02±0.35b). Conclusion: It was observed that the impaired metabolic changes caused by fructose in the brain tissue were partially improved in the EPO-treated group as a result of the decrease in insulin, resistin and increase in adiponectin. Accordingly, since metabolic changes in the brains of rats fed with high fructose content may also occur in humans with fructose intake from various foods, the use of EPO in the medical setting may be recommended by clinicians to reduce the harmful effects on the brain.
Effect of Vitamin E on Ecg, Blood Lipid Peroxidation and Antioxidant Enzyme Activity in Experimentally-Induced Hypothermic Guinea-Pigs
(2004) Mis, Leyla; Meral, İsmail
Bu çalışmasında vitamin E uygulamasının deneysel olarak hipotermi oluşturulan kobaylarda elektrokardiyogram (EKG) bulguları, serum lipit peroksidasyonu ve antioksidan enzim düzeyleri üzerine etkileri araştırıldı. Araştırmada 500-800 gr ağırlığında 30 adet sağlıklı erkek kobay (guinea pig) kullanıldı. Bir haftalık adaptasyon süresinden sonra, kobaylar her bir grupta 10 adet olacak şekilde kontrol (K), hipotermik (H) ve vitamin E uygulanan hipotermik (Vit E+H) olmak üzere 3 deneme grubuna ayrıldı. Analizler yapılmadan önce Vit E+H grubundaki kobaylara oral olarak 4 gün boyunca 460 mg/kg vitamin E verildi. Diğer iki gruba (K ve H) vitamin E verilmedi. Son vitamin E uygulamasından 24 saat sonra H ve Vit E+H gruplarında yer alan kobaylar herhangi bir anestezik veya tarankilizan madde verilmeden boyunlarının üst kısmına kadar soğuk su (10-12 0C) içerisine sokuldular ve 5 dakika soğuk su içerisinde tutuldular. Kontrol grubundaki kobaylar ise aynı yöntemle vücut ısısındaki (37 0C) su içerisinde 5 dakika tutuldular. Su içerisinden çıkarılan kobayların rektal vücut ısıları termometre ile ölçüldü ve EKG'leri çekildi. EKG çekimini takiben malondialdehit (MDA) süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve redükte glutatyon (GSH) tayini için eter ile anestezi edilen hayvanların kalbinden biri EDTA'lı olmak üzere iki plastik tüpe kan örnekleri alındı. Hipotermi oluşturulan grupta vücut ısısı önemli oranda azaldı. Üçüncü derece atriovetriküler blok ve Osborn J dalgası (erken ventriküler repolarizasyon) şekillendi. Ayrıca bu kobaylarda MDA değerinin önemli ölçüde arttığı, SOD değerinin değişmediği, GSH ve GSH-Px değerlerinin ise önemli ölçüde azaldığı görüldü. Vitamin E uygulanmış hipotermik grupta bulunan kobaylarda vücut ısısı önemli derecede düşmesine rağmen, hipotermi grubunda gözlenen bulgulara bu grupta rastlanmadı. Böylece, vitamin E'nin hipoterminin oluşturduğu lipit peroksidasyonunu ve EKG'deki değişiklikleri önlediği, ve ayrıca antioksidan savunma sistemini güçlendirdiği sonucuna varıldı.
Effect of Vitamin E on Ecg, Blood Lipid Peroxidation and Antioxidant Enzyme Activity in Experimentally-Induced Hypothermic Guinea-Pigs
(2003) Mis, Leyla; Meral, İsmail
Bu çalışmasında vitamin E uygulamasının deneysel olarak hipotermi oluşturulan kobaylarda elektrokardiyogram (EKG) bulguları, serum lipit peroksidasyonu ve antioksidan enzim düzeyleri üzerine etkileri araştırıldı. Araştırmada 500-800 gr ağırlığında 30 adet sağlıklı erkek kobay (guinea pig) kullanıldı. Bir haftalık adaptasyon süresinden sonra, kobaylar her bir grupta 10 adet olacak şekilde kontrol (K), hipotermik (H) ve vitamin E uygulanan hipotermik (Vit E+H) olmak üzere 3 deneme grubuna ayrıldı. Analizler yapılmadan önce Vit E+H grubundaki kobaylara oral olarak 4 gün boyunca 460 mg/kg vitamin E verildi. Diğer iki gruba (K ve H) vitamin E verilmedi. Son vitamin E uygulamasından 24 saat sonra H ve Vit E+H gruplarında yer alan kobaylar herhangi bir anestezik veya tarankilizan madde verilmeden boyunlarının üst kısmına kadar soğuk su (10-12 0C) içerisine sokuldular ve 5 dakika soğuk su içerisinde tutuldular. Kontrol grubundaki kobaylar ise aynı yöntemle vücut ısısındaki (37 0C) su içerisinde 5 dakika tutuldular. Su içerisinden çıkarılan kobayların rektal vücut ısıları termometre ile ölçüldü ve EKG'leri çekildi. EKG çekimini takiben malondialdehit (MDA) süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve redükte glutatyon (GSH) tayini için eter ile anestezi edilen hayvanların kalbinden biri EDTA'lı olmak üzere iki plastik tüpe kan örnekleri alındı. Hipotermi oluşturulan grupta vücut ısısı önemli oranda azaldı. Üçüncü derece atriovetriküler blok ve Osborn J dalgası (erken ventriküler repolarizasyon) şekillendi. Ayrıca bu kobaylarda MDA değerinin önemli ölçüde arttığı, SOD değerinin değişmediği, GSH ve GSH-Px değerlerinin ise önemli ölçüde azaldığı görüldü. Vitamin E uygulanmış hipotermik grupta bulunan kobaylarda vücut ısısı önemli derecede düşmesine rağmen, hipotermi grubunda gözlenen bulgulara bu grupta rastlanmadı. Böylece, vitamin E'nin hipoterminin oluşturduğu lipit peroksidasyonunu ve EKG'deki değişiklikleri önlediği, ve ayrıca antioksidan savunma sistemini güçlendirdiği sonucuna varıldı.
Effect of Wheatgrass on Dna Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
(Soc Chilena Anatomia, 2018) Mis, Leyla; Comba, Bahat; Uslu, Sema; Yeltekin, Asli
This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 mlikg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.
Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene
(Kafkas Univ, veteriner Fakultesi dergisi, 2016) Comba, Bahat; Oto, Gokhan; Mis, Leyla; Ozdemir, Hulya; Comba, Arzu
In this study was investigated effects of borax (BX) on inflammation markers, haematolojical parameters and total oxidant (TOS)-antioxidant status (TAS) in rats applied 3-methylcholanthrene (3-MC). In this research a total of 24 Wistar Albino rats were used. They were divided into 4 groups each containing 6 rats. 1st group was separated as a control group. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose to the 2nd group with i.p. way. BX was given to 3rd group 300mg/L/day dose with drinking water during 150 days. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose with i.p. way and BX were given with drinking water during 150 days to 4th group. At the end of the study blood analysis, tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) levels in 3-MC group; TOS and oxidative stress index (OSI), platelet (PLT) levels in 3-MC and 3-MC+BX groups showed significantly increases when compared to other groups. It was determined that lymphocytes % (LY%) of ever 3 groups were significantly higher; however, neutrophil % (NEU%) were significantly fewer according to control group. Haemoglobin (HGB) and hematocrit (HCT) values of 3-MC+BX groups showed significantly decrease according to other groups (P <= 0.05). Mean corpusculer volume (MCV) in 3-MC and 3-MC+BX groups showed significantly decrease when compared to other groups (P <= 0.05). As a result, in case of exposure to 3-MC, long-term use of BX with oral ways may not decrease oxidative stress, may changes haematolojical parameters such as, WBC, LY%, NEU%, PLT, HGB, HCT, MCV. However, these changes remain within physiological limits. Even so, in the use of BX should be considered use of iron. Furthermore, BX with the abovementioned dosage may be used to reduce the levels of TNF-alpha, IL-1 beta, IL-6 being inflammation and cancer markers.
The Effects of Grape Seed Extract on Insulin, Adiponectin and Resistin Levels in Diabetic Rats
(2021) Yılmaz, Halil Cumhur; Mert, Nihat; Mis, Leyla; İrak, Kıvanç; Mert, Handan; Söğütlü, İnci
In this study, the effects of grape seed extract on insulin, adiponectin and resistin levels in diabetic rats were aimed to investigate. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats were used. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats randomly divided into four groups of eight each: control group (C), group with diabetes (DM), grape seed extract group (GSE), diabetes mellitus and grape seed extract group (DM+GSE). The diabetic group 45 mg/kg single dose of streptozotocin was administered intraperitoneally, and the grape seed extract groups (DM+GSE and GSE) grape seed extract was given orally every day with an intragastric tube for 20 days (0.6 ml/rat). Blood samples were taken from the hearts of rat’s end of the experiment. The sera obtained were used for insulin, adiponectin and resistin analysis measured via ELISA with commercial kits. The insulin and adiponectin levels of the rats in the diabetes + grape seed extract group were found to be higher than those in the diabetes group. There was no significant change in the resistin level differences between the groups. As a result; It is possible to say that grape seed extract has an increasing effect on insulin level and adiponectin levels of diabetic rats are decreased due to diabetes and it has no significant effect on resistin levels. Detected by the positive effects of grape seed extracts, it may be useful to use for the regulating of the diabetes in human.
The Effects of Nutritional Differentiation and Duration on Levels of Inflammation Markers in the Experimental Acute Colitis Model
(Wiley, 2017) Emlik, Huseyin; Bayiroglu, Fahri; Mis, Leyla
Effects of Protocatechuic Acid Against Cisplatin-Induced Neurotoxicity in Rat Brains: an Experimental Study
(Taylor & Francis Ltd, 2024) Mert, Handan; Kerem, Ozge; Mis, Leyla; Yildirim, Serkan; Mert, Nihat
Aims/Objectives: Cisplatin (CIS) is widely used in the treatment of various malignant tumors. The aim of study is to determine the potential protective effects of protocatechuic acid (PCA) on the brain in neurotoxicity induced by CIS in rats.Materials and methods: Forty rats were divided into four groups: 1-Control group: 2- PCA group: PCA was administered orally at a dose of 100 mg/kg/day for 5 weeks. 3-CIS group: 5 mg/kg/week of CIS was administered intraperiteonally 4-PCA + CIS group: The rats were given PCA orally daily for 5 weeks and CIS of 5 mg/kg/week. The brain tissues were used for histopathological examinations and for total antioxidant capacity (TAC), total oxidative state (TOS), oxidative stress index (OSI), tumornecrosis factor-alpha (T NF-alpha), interleukin 6 (IL-6) Interleukin 1 beta (IL-1 beta), acetylcholinesterase (AChE), glutamate, gamma aminobutyric acid (GABA), dopamine analyzes in ELISA. WBC, RBC, hemoglobin and hematocrit levels were measured.Results: PCA + CIS group compared to CIS group TOS, OSI, T NF-alpha, IL-6, IL-1 beta, AChE, glutamate, WBC levels decreased significantly, while TAC and GABA levels increased statistically significant. With this study, P CA corrected the deterioration in the oxidant / antioxidant status, suppressed neuro-inflammation, decreased AChE activity, partially normalized neurotransmitters, and decreased the increased WBC count. Necrosis seen in the CIS group in histopathological examinations was never seen in the PCA + CIS group.Conclusions: PCA may provide therapeutic benefit when used in conjunction with CIS.
Effects of Zinc Supplementation on Dna Damage in Rats With Experimental Kidney Deficiency
(Humana Press inc, 2017) Yegin, Sevim Ciftci; Dede, Semiha; Mis, Leyla; Yur, Fatmagul
This study was carried out to determine the effect of zinc on oxidative DNA damage in rats with experimental acute and chronic kidney deficiency. Six groups of five Wistar-Albino rats each were assigned as controls (C), acute kidney deficiency (AKD), zinc-supplemented (+Zn), acute kidney deficiency, zinc-supplemented (AKD + Zn), chronic kidney deficiency (CKD) and zinc-supplemented chronic kidney deficiency (CKD + Zn). The levels of 8-Oxo-2'-deoxyguanosine (8-OHdG) were determined, being the lowest in the CKD group (p < 0.05), higher in the C group than those of rats with CKD but lower than that of all the other groups (p < 0.05). There were no significant differences between the controls and the CKD + Zn group, or between the AKD and the +Zn groups. Among all groups, the highest 8-OHdG level was found in the AKD + Zn group (p < 0.05). DNA damage was greater in acute renal failure than in rats with chronic renal failure. The DNA damage in the zinc group was significantly higher than in the controls.
Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows With Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus Aureus
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Kurt, Serdal; Eski, Funda; Mis, Leyla; Demir, Pinar Ayvazoglu
The aim of this study was to investigate effect of Staphylococcus aureus on oxidative stress status (TAS, TOS, OSI), immune system (IL-1 beta, IL-6, TNF-alpha), and mineral (Mg, Fe, Zn, Cu, Na and Ca) concentrations in milk and serum of cows with mastitis. The cows were allocated to three groups according to mammary health status as follows: healthy (Group 1), clinical (Group 2) and subclinical mastitis cows (Group 3). IL-1 beta levels in serum and milk increased in Group 2 compared to Group 1 and Group 3 (P<0.001; P<0.05). Milk IL-6 level was greater in Group 3 and Group 2 than in Group 1 (P<0.01). Blood TNF-alpha (P<0.001), TOS and OSI levels (P<0.01) were higher in Group 2 than other groups. Milk TNF-alpha level increased and blood TAS level decreased in Group 2 compared to Group 1 (P<0.05). Milk TOS (P<0.01) and OSI (P<0.05) levels increased in Group 3 compared to Group 1. Blood and milk Mg levels increased in Group 1 (P<0.05) and Group 2 (P<0.001), respectively. Milk Fe (P<0.01) and Na levels were greater in Group 2 (P<0.001). Blood Zn level was lower in Group 2 compared to Group 3 (P<0.05). While blood Cu level decreased in Group 1 compared to other groups, milk Cu level decreased in Group 3 compared to other groups (P<0.001). In conclusion, S. aureus had significant effects on oxidative stress, cytokine and mineral levels in milk and blood serum of cows with clinical and subclinical mastitis. However, since there were specific changes only in milk OSI and IL-6 levels according to other parameters, it is thought that milk IL-6 and OSI levels may be a diagnostic tool in the detection of subclinical mastitis.
The Evaluation of Retinol, Α-Tocopherol, Cholecalciferol and Reproductive Hormones Levels After Administrated Allium Schoenoprasum L. Ethanol Extract and Acrylamide in the Female Rats
(2020) Mis, Leyla; Belhan, Saadet; Ayan, Adnan; Huyut, Zübeyir; York, Ibrahım Hakkı; Yaşar, Semih
This study was carried out to determine the levels of retinol, α-tocopherol, cholecalciferol andreproductive hormones (follicle stimulating hormone - FSH, luteinizing hormone - LH,progesterone, estradiol) in the female rats administrated Allium schoenoprasum L. ethanol extract(ASLEE) and acrylamide. The study was performed on thirty-two Wistar albino female rats (200-220 grams). The rats were divided into 4 groups with an equal number. Serum LH values werehigher in the ASLEE group compared to the other groups. Control group: No treatment wasperformed. Acrylamide group: Acrylamide was administrated by gastric gavage at a dose of 25 mg/ kg daily. ASLEE group: ASLEE was administrated by gastric gavage at a dose of 200 mg / kgdaily. Acrylamide + ASLEE group: Acrylamide was administrated by gastric gavage at a dose of25 mg / kg daily. Then ASLEE was administered by gastric gavage at a dose of 200 mg / kg perday. Serum FSH and LH values were significantly lower in the acrylamide group compared to theother groups. Serum LH values in the acrylamide + ASLEE group were significantly restoredcompared to the acrylamide group. Serum estradiol values were partially lower in the acrylamidegroup compared to other groups, but there was no significant difference between the groups. Serumprogesterone values in the acrylamide group were significantly lower than the control group. Serumprogesterone values were higher in the acrylamide + ASLEE group compared to the acrylamidegroup. As a result, the levels of retinol, α-tocopherol, cholecalciferol and reproductive hormones inASLEE were determined in this study. In addition, when ASLEE was applied with acrylamide, therate of change in the relevant parameters was determined.