Browsing by Author "Mukemre, Muzaffer"

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Centaurea Karduchorum Boiss. From Eastern Anatolia: Phenolic Composition, Antioxidant and Enzyme Inhibitory Activities
(Elsevier Gmbh, Urban & Fischer verlag, 2015) Dalar, Abdullah; Uzun, Yusuf; Mukemre, Muzaffer; Turker, Musa; Konczak, Izabela
The phenolic composition of lyophilized hydrophilic extracts obtained from root, stem, leaf and flower of Centaurea karduchorum Boiss. a medicinal plant endemic to Eastern Anatolia (Turkey) used as antidiabetic agent was characterized for the first time. Liquid chromatography mass spectrometry (LC/MS) analysis revealed that luteolin derivatives and chlorogenic acid were the dominating compounds contributing, respectively, from 16.1% (root) to 33.7% (stem) and from 7.3% (flower) to 12.0% (root) of total phenolics. Total reducing capacities of the extracts varied from 24.1 (root) to 38.5 (flower) mg gallic acid Eq./g DW [Folin-Ciocalteu assay] and from 274.0 (root) to 441.0 (stem) mu mol Fe2+/g DW [FRAP assay]. The oxygen radical absorbance capacity (ORAC assay) values varied from 930.5 (root) to 1853.5 (stem) mu mol trolox Eq./g DW. The leaf extract exhibited a pronounced inhibitory activity towards alpha-glucosidase (IC50: 0.63 +/- 0.00 mg/ml) and a weak inhibitory activity towards amylase (IC50: 14.63 +/- 0.67 mg/ml), which suggests a potential to reduce postprandial hyperglycaemia and supports its traditional use as antidiabetic agent. (C) 2015 Elsevier GmbH. All rights reserved.
Determining Phylogenetic Relationships of Some Endemic and Rare Astragalus Taxa in the Van Lake Basin
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2023) Genli, Gulistan; Tekbudak, Ilhan Kaya; Usta, Mustafa; Mukemre, Muzaffer
Astragalus, the most diverse genus in the world, contains about 3000 different taxa. This genus has 489 taxa and 63 sections in Turkey, giving it a 51% endemism rate. 82 Astragalus taxa were collected from the Van Lake Basin in 2019 for this research. These species' morphological diagnoses revealed that four of them were rare and 17 of them were endemic. In this research, the universal primers ITS4 and ITS5 were used to amplify the ITS1 and ITS2 sections, which included the 5.8S gene of rDNA. Different programs (SnapGene, CLC DNA Workbench) were used to analyze the genome information of species in the genus Astragalus. The DNA sequences of Astragalus species have been uploaded to the GenBank database of the National Center for Biotechnology Information (NCBI) (Maryland, USA), which is open to all researchers worldwide. The length of the ITS in the study, including the 5.8S sequence, varied from 669 to 687 bp. The results showed that the phylogenetic tree combined with the most stable secondary (20) structure obtained from the universal ITS4 and ITS5 primers is an effective tool for the identification of Astragalus taxa.
Electrochemical Biosensing: From Interaction Between Epilobium Species and Dna To an Approach To Detect New Electroactive Components in Plant Extracts and Their Effects on Dna
(Springer Heidelberg, 2024) Subak, Hasret; Dalar, Abdullah; Mukemre, Muzaffer; Ozkan-Ariksoysal, Dilsat
Here, a simple and label-free biosensor based on disposable pencil graphite electrode (PGE) was developed for the first time to investigate the interaction between Epilobium sp. plant extract containing active compounds (quercetin and kaempferol) and DNA using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The solution of extract was interacted with calf thymus double-stranded DNA (dsDNA) which immobilized onto the biosensor surface by simple adsorption. The effect of Epilobium on double-stranded DNA (dsDNA) was then analyzed by monitoring the changes in electrochemical signals arising from both guanine bases in DNA and the electroactive compounds in Epilobium extract. Epilobium content was also detected by using HPLC/MS/MS technique, and the gained results were compared with those obtained by electrochemical method. It was found that both methodologies supported each other in terms of active ingredients in Epilobium sp. Numerous factors affecting the extract-DNA interaction were optimized such as Epilobium concentration, interaction time. The developed DNA sensor can well detect Epilobium extract-DNA interaction in 60-min detection time with 26 ng of detection limit in 50 mu L of sample volume with a linear range from 1 to 10 mu g/mL. This study includes alternative method that can be used to detect new electroactive components present or to be found in different plant extracts and their interactions with DNA.
Ethnobotanical Study on Medicinal Plants in Villages of Catak (van-Turkey)
(Elsevier Ireland Ltd, 2015) Mukemre, Muzaffer; Behcet, Lutfi; Cakilcioglu, Ugur
Ethnopharmacological relevance: This paper provides significant ethnobotanical information on medicinal plants in the villages of Catak in the Eastern Anatolia Region. Recording such data calls for urgency. This is the first ethnobotanical study in which statistical calculations about plants are carried out by means of FIC method in Eastern (Van) part of Turkey. Aim of the study: This study aims to identify the wild plants collected for medicinal purposes by locals of Catak which is located in the Eastern Anatolia Region of Turkey, and to identify the uses and local names of these wild plants. Materials and methods: A field study had been carried out for a period of approximately 2 years (20102012). During this period, 78 plants taxa were collected. Demographic characteristics of participants, names of the local plants, their utilized parts and preparation methods were investigated and recorded. The plant taxa were collected within the scope of the study; and herbarium materials were prepared. In addition, the relative significance value of the taxa was determined, and informant consensus factor (FIC) was calculated for the medicinal plants included in the study. Results: We have found out in the literature review of the plants included in our study that 78 plant taxa are already used for medicinal purposes while 19 plants are not available among the records in the literature. The most common families are Asteraceae, Apiaceae, Lamiaceae, Rosaceae, Euphorbiaceae, Fabaceae, and Malvaceae. We include in our study and report for the first time the medicinal uses of Alchemilla buseriana Rothm.. Astragalus longifolius Lam., Cephalaria microcephala Boiss., Euphorbia grisophylla M.S. Khan, Fritillaria crassifolia Boiss. & Huet. subsp. kurdica (Boiss. & Noe) Rix, Fritillaria pinardii Boiss., Malabaila lasiocarpa Boiss., Nepeta betonicifolia CA. Mey., Onobrychis altissima Grossh., Onobrychis carduchorum C.C. Townsend, Papaver bracteatum Lindl., Phlomis tuberosa L, Psephellus karduchorum (Boiss.) Wagenitz, Scutellaria orientalis L subsp. pichleri (Stapf.) Edmondson, Stachys kurdica Boiss. & Hohen var. kurdica, Tanacetum kotschyi (Boiss.) Grierson, Tanacetum zahlbruckneri (Nib.) Grierson, Turanecio eriospermus (DC.) Hamzaoglu, Verbascum pyramidatum M.Bieb. Names of local plants in Turkey vary especially due to vernaculars. The plants that the locals of Catak use are called with the same or different local names in various parts of Anatolia. Conclusion: We found out that locals living in the research area use for therapeutic purpose 78 plants taxa which belong to 22 families. Turkish citizens with different ethnic backgrounds took the questionnaire. These people use these wild plants in treatment of several diseases. Comparison of the data obtained in this study with the experimental data obtained in the previous laboratory studies on the wild plants which grow in Catak proved ethnobotanical usages to a great extent. Literature review indicated that the therapeutic plants that grow in Catak are used in different parts of the world for the treatment of similar diseases. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
The Phenolic Profile and Biological Activities of the Wild-Edible Mushrooms Helvella Leucopus and Morchella Pulchella
(Springer, 2021) Acar, Ismail; Blando, Federica; Gul, Bahar; Greco, Alessandra; Mukemre, Muzaffer; Uzun, Yusuf; Dalar, Abdullah
Morchella pulchellaClowez & Franc. Petit. andHelvella leucopusPers. are among the most utilized naturally grown wild-edible mushroom species in Eastern Anatolia, Turkey. This study focused on revealing their phenolic profiles using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), antioxidant (hydrogen atom and single electron-transfer mechanisms), and enzyme inhibition (acetylcholinesterase, amylase, glucosidase, and lipase) activities of lyophilized sequential (n-hexane, ethanol, methanol, and water, respectively) extracts and traditional preparations (infusion and decoction) obtained from the fruiting bodies of these mycomaterials. Our chromatographic studies revealed thatM. pulchellaextracts are dominated by caffeic acid, whileH. leucopusextracts contained significant levels of protocatechuic acid as a major biologically active compound (excluding n-hexane fractions). The most active antioxidant and enzyme inhibitory extracts were from ethanol (or methanol) inM. pulchellaand water (or infusion) inH. leucopus. Data obtained from our study showed that hydrophilic extracts ofM. pulchellaandH. leucopuswere significant sources of antioxidants and enzyme inhibition activities and that they might be used as raw materials in the food, nutraceutical, biotherapeutic and pharmaceutical industries. [GRAPHICS] .
Phytochemical Pro File and Biological Activities of Anatolian Plantain (Plantago Anatolica)
(Elsevier, 2020) Mukemre, Muzaffer; Konczak, Izabela; Uzun, Yusuf; Dalar, Abdullah
The phytochemical profile and potential biological activities of Plantago anatolica Tutel & R. Mill., a traditional endemic food plant of Eastern Anatolia, Turkey, were studied. The study included analysis of phytochemical and mineral compositions of lyophilized sequential extracts (n-hexane, chloroform, ethyl acetate, acetone, ethanol and water) and traditional preparations (herbal infusion, decoction and cold water extraction) obtained from aerial parts of the P. anatolica plant, followed by evaluation of their antioxidant capacities and inhibitory activities towards enzymes involved in metabolic syndrome. Chromatographic studies showed that phenolic compounds (caffeic and chlorogenic acids, apigenin and kaempferol), and fatty acids (palmitic and linolenic acids) and an aromatic compound benzotiazole were the major phytochemicals. The ethanol extract and the herbal infusion extracted the most caffeic acid. These two preparations showed significant oxygen radical absorbance capacity (1.9 and 1.0 mmol Trolox equivalent/g dw, respectively), high ferric reducing antioxidant power (0.9 and 0.7 mmol Fe2+/g dw, respectively), 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity (IC50: 0.87 and 0.34 mg/ml, respectively), and ferric chelation activities (IC50: 0.09 mg/ml). Both preparations showed high inhibitory activities towards a-glucosidase (IC50 of 0.8 and 0.6 mg/ml, respectively), and lipase (IC50 of 72 and 44 mu g/ml, respectively) but low inhibitory activities towards a-amylase (IC50: 4.31 and 5.32 mg/ml, respectively). These results highlighted the potential of P. anatolica as a source of phytochemicals with strong antioxidant and enzyme inhibitory properties for application in the food and biopharmaceutical industries.
Screening in Vivo Antioxidant and Haematological Properties of Sumac and Acorn Bioactive Rich Extracts
(Elsevier Science Bv, 2018) Dalar, Abdullah; Dogan, Abdulahad; Bengu, Aydin Sukru; Mukemre, Muzaffer; Celik, Ismail
Sumac (Rhos coriaria L.) and acorn (Quercus brantii Lindl.) are plant species widely used in the Mediterranean countries for various industrial, therapeutic and nutritional purposes. This study aimed to investigate the phytochemical composition (phenolics, volatiles, fatty acids, and minerals) and effects of the extracts obtained from sumac and acorn on antioxidant mechanisms in various tissues (lung, spleen, hearth, and muscle) and several haematological parameters. Bioactive rich extracts containing phenolics (gallic acid and methyl gallate), volatiles (beta-pinene, octanal, limonene, nonanal, beta-caryophyllene, and humulene), fatty acids (oleic, palmitic, and linoleic acids) and mineral compounds (K, Mg, Ca, Si, Na, and B) regulated the levels of lipid peroxidation and antioxidant defence systems, and haematological parameters effectively.
Survey of Wild Food Plants for Human Consumption in Villages of Catak (van-Turkey)
(Natl inst Science Communication-niscair, 2016) Mukemre, Muzaffer; Behcet, Lutfi; Cakilcioglu, Ugur
This study aims to record accumulation of knowledge on plants which are used as food by native people of Catak (Van, Turkey) that has a rich culture and a very natural environment. In addition, the medical uses of these plants were compiled from the literature. Study area was located on the East of Anatolian diagonal, in the Eastern Anatolia Region. Field study was carried out over a period of approximately two years (2010-2012). During this period, 82 vascular plant taxa were collected. The plants were pressed in the field and prepared for identification. A total of 82 food plants belonging to 28 families were identified in the region. In the study being conducted, use of wild plants as food points out interest of people in Catak in wild plants. The fact that a large proportion of edible plants are also being used for medicinal purposes indicates that the use of wild plants has a high potential in the region. The present study shows that further ethnobotanical investigations are worthy to be carried out in Turkey, where most of knowledge on popular food plants are still to discover. In Turkey, the number of ethnobotanic studies is ever-increasing. However, traditional uses of many wild plants have not been recorded yet. In terms of food safety, the adverse effects that may arise due to the use of wild plants without sufficient knowledge must be reported to the native people.
Traditional Medicinal Plants of Agri Province, Turkey
(Elsevier Ireland Ltd, 2018) Dalar, Abdullah; Mukemre, Muzaffer; Unal, Murat; Ozgokce, Fevzi
Ethnopharmacological relevance: This study serves as the first investigation of the knowledge of traditional medicinal plants used in Agri Province. The mountainous and strongly fragmented area, diversity of local flora, limited access to modern medicines because of the people's seminomadic lifestyle, and harsh climatic conditions have prompted the locals to benefit from natural resources, principally plants. Aim of the study: Although the use of medicinal plant is common in the area, documentation and inventory analysis are not available on the plant taxa used in traditional medicine in Agri Province. This study focused on the answer of the following question: What are the most medicinally valuable plant taxa in the province? Therefore, this study aimed to (i) document medicinal plant taxa used in Agri Province; (ii) determine the endemic plant species and their risk categories; (iii) determine the most widely used plant taxa for specific ailments to further contribute to drug discovery; (iv) determine the local names, plant part(s) used, preparation, and utilization methods of these local plant medicines to assist with pharmaceutical studies; and (v) reveal the origin(s) of traditional medicinal knowledge of Agri Province. Materials and methods: Ethnopharmacological field studies were conducted between 2014 and 2016 in 638 locations within Agri Province. Plant samples used in folk medicine were determined through face-to-face interviews with 120 local healers. Traditional medicinal plant samples were collected from wild areas with the help of the local healers and transported to Van Pharmaceutical Herbarium (Van, Turkey) for botanical identification. The statistical analyses of data were conducted using the informant consensus factor (ICF) method. Results: In this study, 118 medicinal taxa belonging to 35 families of plants were determined to be used in Agri Province. Ninety-three different local names were recorded, which mainly described the plant's morphological characteristics. Data analysis (ICF) revealed that the traditional medicinal plants used in Agri Province might offer promising information for new pharmaceuticals. Conclusions: The local population in the study area has a preserved and comprehensive knowledge of traditional medicine because of its limited access to modern medicine, seminomadic lifestyle, isolated geographical position, traditional taste habits, climatic conditions, diversity of local flora, and mountainous and rugged geography. The medicinal plants presented in this study might provide valuable leads for the identification of bioactive compounds for pharmaceutical use and represent a vast and underutilized resource for the development of newer and more efficient pharmacological treatments.