Browsing by Author "Noyan, T"

Now showing 1 - 15 of 15

Alterations in the Biochemical Markers of Renal Function After Sevoflurane Anaesthesia
(Wiley, 2005) Sekeroglu, MR; Kati, I; Noyan, T; Dülger, H; Yalçinkaya, A
Aim: This study has been carried out to see whether renal function is acutely altered in patients undergoing sevoflurane anaesthesia. For this purpose, the urinary levels of markers of renal tubular function, namely leucine amino peptidase (LAP), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and beta-2 microglobulin (beta-2M), and urinary albumin as a predictor of renal glomerular function were measured before and after sevoflurane anaesthesia. Methods: This study was comprised of 20 patients (11 males and nine females) aged 18-55, who underwent various elective surgical procedures under general anaesthesia. Urine samples of all patients were collected before and 1, 2 and 8 h after the anaesthesia. The levels of LAP, GGT, beta-2M, and albumin were then expressed as factored by urinary creatinine. In all patients, the anaesthesia was maintained with sevoflurane (2% end-tidal) at a high flow-rate (6 L/min). Results: Urinary beta-2M and LAP levels after anaesthesia were unchanged (P > 0.05). While urinary GGT and ALP levels were found elevated in the first hour, LDH levels were higher in the second hour (P < 0.05). They returned to normal levels in the later periods after the anaesthesia. Urinary albumin excretion (UAE) was significantly elevated in the second hour after the anaesthesia (P < 0.001). Although UAE was decreased in the eighth hour after the anaesthesia, it still remained higher than the pre-anaesthesia level (P < 0.001). Conclusion: These results suggest that a 2% end-tidal concentration of sevoflurane at a high flow-rate (6 L/min) acutely alters renal glomerular function but does not have a significant acute effect on biochemical markers of renal tubular damage.
Antithyroid Antibody Levels in Patients With Breast Cancer
(Amer Assoc Clinical Chemistry, 2004) Sekeroglu, MR; Dulger, H; Alici, S; Algun, E; Noyan, T
Diagnostic Value of Cytokeratin-18 as a Tumor Marker in Bladder Cancer
(Pergamon-elsevier Science Ltd, 2002) Sekeroglu, MR; Aydin, S; Dülger, H; Yilmaz, Y; Bayrakli, H; Noyan, T
Objectives: The aim of the study was to compare serum levels of cytokeratin-18 of patients with bladder cancer with those of the healthy controls, and to investigate the relation between cytokeratin level and the tumor stage. Design and methods: Serum cytokeratin-18 levels of 38 patients with bladder cancer and of 25 healthy people were determined. Tumor stage was T-1 in 12 patients, T-2 in 9 patients, T-3 in 10 patients and T-4 in 7 patients. The serum cytokeratin-18 levels in these cases were analyzed with respect to the stage of the tumor. Results: Cytokeratin- 18 level in the patient group was found to be significantly higher than that of the control group (p < 0.010) when the groups were totally compared. However, when the levels in patients with different tumor stages were compared with that of the controls, the difference was not significant in patients with stage I and 2 tumors (p > 0.05). Regarding the cut off value as 4.0 ng/mL, sensitivity and specificity for serum cytokeratin-18 were found to be 53% and 72% respectively. When sensitivity was calculated with respect to tumor stages, it was 8% for T-1, 33% for T-2, 90% for T-3 and 100% for T-4. On the other hand, considering higher stage (T-3 and T-4) tumors only, the sensitivity was calculated as 94%, but the sensitivity for lower stage (T-1 and T-2) tumors was 19%. Conclusions: It is clear that serum cytokeratin- 18 level increases in patients with bladder cancer. However, it can only be useful as a tumor marker in the diagnosis of T-3 and higher staged tumors. This study indicated that cytokeratin- 18 does not have any diagnostic value in lower stage bladder cancers. (C) 2002 The Canadian Society of Clinical Chemists. All rights reserved.
The Effect of High Dose Digoxin on Cytokines in Healthy Dogs
(Hindawi Ltd, 2002) Dülger, H; Sekeroglu, MR; Eryonucu, B; Noyan, T; Alici, U
Background: Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta are pro-inflammatory cytokines, causing myocardial dysfunction and a negative inotropic effect. The drugs used to treat heart failure affect the production of cytokines. Digoxin, on which this study was focused, is one of the drugs for the treatment of heart failure. Aim: The present study was designed to examine the early effects of high doses of digoxin on the production of cytokines in healthy dogs. Methods: Digoxin was given parenterally to dogs at 0.15 mg/kg. IL-1beta and TNF-alpha production and levels of digoxin in the serum were measured 0, 12, 24, 48, and 72 h following administration of digoxin. Results: As the levels of serum digoxin taken at 12, 24, 48, and 72 h of administration were considered significantly high compared with preceding values (p<0.001), no notable change in serum IL-1 beta and TNF-alpha levels was observed. Conclusions: These results suggest that high doses of digoxin do not cause a significant cytokine production in heart muscle in the early phase.
Effects of Erythropoietin and Pentoxifylline on the Oxidant and Antioxidant Systems in the Experimental Short Bowel Syndrome
(Wiley, 2003) Noyan, T; Onem, O; Sekerglu, MR; Köseoglu, B; Dülger, H; Bayram, I; Bakan, V
In this study, we investigated the effects of erythropoietin (Epo), and pentoxifylline (Ptx) on the oxidant and antioxidant systems in the experimental short bowel syndrome. Spraque-Dawley rats were divided into four groups and all animals underwent 75% small bowel resection. Group E was treated with 500 IU kg(-1) Epo subcutaneously (s.c.), group P with 50 mg kg(-1) day(-1) s.c. Ptx and group E + P with 500 IU kg(-1) s.c. Epo plus 50 mg kg(-1) day(-1) s.c. Ptx for a period of 28 days. In group C, which is the control group, no drug treatment was given. At the end of 28 days the experimented rats were killed and ileum samples excised for biochemical and histopathological testing. Malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were determined in ileum homogenates. When compared to group C, the MDA and GSH-Px levels were significantly decreased (p < 0.05), but SOD activitiy was not changed (p > 0.05) in groups P and E + P, whereas both MDA and SOD and also GSH-Px activities were not changed significantly in group E (p > 0.05). The average villous length, crypt depth, muscular thickness and mucosal length were measured in all groups. The average crypt depth and mucosal length were statistically higher in the group P than group C (p < 0.001, p < 0.01, respectively). In addition, the crypt depth was statistically higher in both E and E + P groups as compared to group C (p < 0.001, p < 0.01, respectively). Therefore, our study indicates that Ptx may be more effective than Epo in reducing lipid peroxidation. Moreover, we considered that Ptx may give this protective effect by inhibiting the free oxygen radicals to a greater extent than developing the antioxidant capacity. Copyright (C) 2002 John Wiley Sons, Ltd.
Histamine Contents of White Cheeses Obtained From Van Retail Markets
(indian veterinary Journal, 2004) Noyan, T; Ekici, K; Coskun, H; Dulger, HH
Mild Renal Injury in Behcet's Disease
(Taylor & Francis Ltd, 2004) Sahin, I; Noyan, T; Karaman, E; Yalçinkaya, AS; Metin, A; Akpolat, T
Aim: The aim of this study is to investigate the frequency of microalbuminuria and abnormal urinary beta(2)-microglobulin excretion in patients with Behcet's disease (BD). Materials and Methods: Twenty-eight patients and 27 healthy controls were included in this study. Urine albumin/creatinine and beta(2)-microglobulin/creatinine ratios were calculated. Results: The frequency of microalbuminuria and abnormal urinary beta(2)-microglobulin excretion was higher among patients with BD than in control group, but this was not statistically significant (p>0.05). Conclusion: Microalbuminuria and abnormal beta(2)-microglobulin excretion are markers of renal injury, which have not been investigated in BD previously. Renal injury in BD is more frequent than has been recognized and it is most often in mild nature.
Note on Histamine Levels in Turkish Style Fermented Sausages
(Elsevier Sci Ltd, 2004) Ekici, K; Sekeroglu, R; Sancak, YC; Noyan, T
The histamine contents of Turkish style fermented sausages were determined on 46 samples of five different brands obtained from retail stores in Van in Turkey. Histamine was found in all samples in the range 19.64-87.47 mg/kg (mean 32.13 mg/kg). Histamine levels in the brands differed significantly (P < 0.01). The results suggest that the histamine levels in the sausages were not hazardous in terms of public health although they may be a potential risk to sensitive individuals. (C) 2004 Elsevier Ltd. All rights reserved.
The Oxidant and Antioxidant Effects of 25-Hydroxyvitamin D3 in Liver, Kidney and Heart Tissues of Diabetic Rats
(Springer, 2005) Noyan, T; Balaharoglu, R; Kömüroglu, U
Previous studies have implicated protective effects of vitamin D on insulin secretion and pancreas beta cell function. The goal of the present study is to determine if a combination therapy of 25-hydroxyvitamin D3 and insulin had any advantage over insulin therapy alone on lipid peroxidation and antioxidant activity in the streptozotocin (STZ)-induced diabetic rat. The lipid peroxidation product, thiobarbituric acid-reacting substances (TBARS), was measured to assess free radical activity in the heart, kidney and liver tissues. The enzymatic activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) were measured as indicators of antioxidation in these tissues. Sprague-Dawley rats were made diabetic with a single injection of STZ (75 mg/kg i.p.). Rats were separated into three groups, each containing 10 animals: Group 1, non-diabetic and no drug treatment was given; Group 2, diabetic rats were treated with 3 IU/day subcutaneous (s.c.) insulin; and Group 3, diabetic rats were treated with 3 IU/day (s.c.) insulin plus 1 mg/kg/day per oral (p.o.) 25-hydroxyvitamin D3 for a period of 4 weeks. At the end of the study, TBARS contents of the liver, kidney and heart tissues in Groups 2 and 3 were found to be significantly increased as compared to Group 1 (P < 0.05) and kidney MDA levels in Group 3 were also significantly increased as compared to Group 2 (P < 0.05). The SOD and CAT contents of the heart in Group 2 were significantly increased as compared to Groups 1 and 3 (P < 0.05). GSH-Px activity was unaltered in all groups (P > 0.05). We suggest that a combination of insulin with 25-hydroxyvitamin D3 treatment would not be more beneficial than the use of insulin alone in antioxidant defence of diabetic liver and kidney tissues.
Oxidants and Antioxidants in Tonsillar and Adenoidal Tissue in Chronic Adenotonsillitis and Adenotonsillar Hypertrophy in Children
(Elsevier Ireland Ltd, 2006) Kiroglu, AF; Noyan, T; Oger, M; Kara, T
Objective: The aim of the study is to determine the possible role of oxidants and antioxidants in the pathogenesis of chronic adenotonsillitis and adenotonsillar hypertrophy in children. Patients and methods: The children were divided into infection and hypertrophy groups, which were comparable according to age and gender distribution. The infection group was consisted of 20 children with the diagnosis of chronic adenotonsillitis and the hypertrophy group was made up of 19 childen with adenotonsillar hypertrophy to whom adenotonsillectomy was performed. Preoperative blood levels of erythrocyte MDA, serum MDA, erythrocyte catatase and serum catatase, and adenoidal and tonsillar tissue levels of MDA and catatase were studied. Results: There were significant increase in tonsil MDA, adenoid MDA, tonsil catalase and adenoid catalase levels in infection group (p < 0.05). Conclusion: Oxidants and antioxidants are found to have an important rote in the pathogenesis of adenotonsillar hypertrophy and chronic adenotonsillitis. These findings strengthen the hypothesis that indicates adenotonsillar hypertrophy and chronic adenotonsillitis are different diseases of the same tissues. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
The Prevalence of Pancreatitis in Organophosphate Poisonings
(Arnold, Hodder Headline Plc, 2002) Sahin, I; Onbasi, K; Sahin, H; Karakaya, C; Ustun, Y; Noyan, T
Objective: The aim of this study was to evaluate the prevalence of pancreatitis in cases of organophosphate (OP) poisonings admitted to Yuzuncu Yil University Teaching Hospital over an 18-month period. Materials and Methods: A total of 47 patients of acute poisoning with OP insecticides attended the Emergency Department of the Yuzuncu Yil Medical School Hospital, from May 1999 to December 2000, and were prospectively studied. Serum amylase and lipase levels were studied with colorimetric assay. Serum SGOT, SGPT, LDH, CPK, K levels, leukocyte count and total hospitalization days were also evaluated. Results: Four of 47 patients had obviously elevated amylase and lipase levels (amylase > 300 U/L, lipase > 60 U/L). Only two of the patients with amylase levels between 100 and 300 V/L had also elevated levels of lipase. None of the patients with normal amylase levels had elevated levels of lipase. A total of 12.76% was diagnosed as acute pancreatitis. Conclusion: Acute pancreatitis as a complication of OP intoxication is not a rare condition. In order to improve the outcome of OP poisoning, early diagnosis of acute pancreatitis is important and serum levels of amylase and lipase should be routinely considered carefully. In acute pancreatitis, serum levels of SGOT, SGPT, LDH and leukocyte counts may also be found to be elevated. However, serum K levels are only slightly decreased.
Pro-Inflammatory Cytokines in Turkish Children With Protein-Energy Malnutrition
(Carfax Publishing, 2002) Dülger, H; Arik, M; Sekeroglu, MR; Tarakçioglu, M; Noyan, T; Cesur, Y; Balahoroglu, R
BACKGROUND: Protein-energy malnutrition (PEM) results from food insufficiency as well as from poor social and economic conditions. Development of PEM is due to insufficient nutrition. Children with PEM lose their resistance to infections because of a disordered immune system. It has been reported that the changes occurring in mediators referred to as cytokines; in the immune system may be indicators of the disorders associated with PEM. Aims: To determine the concentrations of pro-inflammatory cytokines in children with PEM, and to find out whether there was an association with the clinical presentation of PEM. Methods: The levels of serum total protein, albumin, tumour necrosis factor-a, and interleukin-6 were measured in 25 patients with PEM and in 18 healthy children as a control group. PEM was divided into two groups as kwashiorkor and marasmus. The kwashiorkor group consisted of 15 children and the marasinus group consisted of 10 children. Results: Levels of serum total protein and albumin of the kwashiorkor group were significantly lower than both the marasmus group and controls (p < 0.05). In view of tumour necrosis factor-a levels, there was no difference between groups (P > 0.05). While levels of interleukin-6 in both the marasmus group and the kwashiorkor group were significantly higher compared with controls (p < 0.05), there was no significant difference between the groups of marasmus and kwashiorkor (p > 0.05). Conclusions: It was observed that the inflammatory response had increased in children with malnutrition.
Serum Levels of Leptin and Proinflammatory Cytokines in Patients With Gastrointestinal Cancer
(Blackwell Publishing Ltd, 2004) Dülger, H; Alici, S; Sekeroglu, MR; Erkog, R; Özbek, H; Noyan, T; Yavuz, M
The aim was to investigate the serum levels of leptin, TNFalpha, IL-1 beta, IL-6, insulin, and growth hormone in patients with upper gastrointestinal cancer and cachexia. A total of 39 patients with various advanced stage (stage IV) gastrointestinal malignancies were enrolled. These cancer patients were divided into two groups according to the presence or absence of cachexia. Fifteen healthy adults were recruited as the control group. Body mass index (BMI; kg/m(2)) was calculated. Serum leptin, turnout necrosis factor (TNF)-alpha interleukin (IL)-1 beta, interleukin (IL)-6, growth hormone, insulin, glucose, triglyceride, total protein, albumin, erythrocyte sedimentation rate, and CRP were measured. In both cancer groups (cachectic and non-cachectic) body mass index and serum leptin levels were lower than controls (p < 0.001). Serum IL-1 beta, IL-6, and growth hormone levels were higher in both cachectic and non-cachectic groups than those of controls (p < 0.05). Serum TNF-alpha level in non-cachectic group was also significantly higher than in control group (p < 0.01). There is no significant difference between three groups in terms of insulin resistance as assessed by HOMA index. Our results showed that some proinflammatory cytokine levels were increased and leptin level was decreased due to upper gastrointestinal cancers. Increased cytokine levels may lead to decreased food intake and caused a weight loss. (C) 2004 Blackwell Ptiblishing Ltd.
The Serum Vitamin C Levels in Behcet's Disease
(Yonsei Univ Coll Medicine, 2003) Noyan, T; Sahin, I; Sekeroglu, MR; Dülger, H
Behcet's disease (BD) is a chronic inflammatory disorder of unknown aetiology, and recognised as a multi-system vasculitis. It has been postulated that an imbalance of the oxidant and antioxidant systems related to the disease are important in its pathogenesis. Previous publications have reported increased levels of enzymatic antioxidant defence systems in patients with BD. The non-enzymatic antioxidant systems, including vitamin C and uric acid, were looked for in the present study. For this aim, the serum malondialdehyde (MDA), an end product of lipid peroxidation, and vitamin C and uric acid, as endogenous antioxidants, were determined in 20 patients with BD (11 in active and 9 in inactive periods) and 20 healthy subjects. The MDA level was significantly higher in both the active and inactive period patients compared with the control group (p < 0.001, p < 0.05, respectively). The MDA level was also significantly higher in the active period patients compared with the inactive period patients (p < 0.05). The vitamin C levels were significantly lower in both the active and inactive period patients compared with the control group (p < 0.001, p < 0.05, respectively). There was no significant difference in the vitamin C level between the active and inactive period patients (p > 0.05). There was also no significant difference in uric acid levels between the groups (p > 0.05). In the patients group, a negative correlation was found between the levels of serum MDA and vitamin C (r=-0.517; p < 0.05). Our results indicate that decreased vitamin C and increased MDA levels reflect the increased levels of oxidative stress in BD patients, and this situation may be important in relation with its pathogenesis.
A Study on the Determination of Histamine Levels in Herby Cheese
(A V A Agrarverlag, 2005) Sancak, YC; Ekici, K; Isleyici, O; Sekeroglu, R; Noyan, T
Cheese, like other fermented foods, may contain histamine as result of decarboxylation of histidine by microorganisms. Histamine is either psychoactive or vasoactive and may cause problems to some consumers. Histamine, has been implicated as the causative agent in several outbreaks of food poisoning. This study was conducted to determine the histamine contents of Herby cheese marketed in Van area, Turkey. Histamine levels of 47 Herby cheese samples purchased from retail stores were quantified by fluorometric o-phthalaldehyde method. Histamine was found in all samples. Histamine concentration varied from 25.62 to 957.62 mg/kg. Mean was 211.82 +/- 206.74 mg/kg. It can be said that the histamine levels in Herby cheeses of 6.38% examined in this study may be hazardous for public health. In conclusion, histamine formation should be controlled by strict use of good hygiene in both raw material and manufacturing environment, with corresponding inhibition of spoiling microorganisms.