Browsing by Author "Noyan, Tevfik"

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8-Ohdg Levels in Patients With Allergic Rhiniti
(Yerkure Tanitim & Yayincilik Hizmetleri A S, 2011) Onbasi, Kevser; Noyan, Tevfik; Cebi, Aysegl; Kiroglu, Faruk
Objective: Respiratory allergies like allergic rhinitis and allergic asthma are more frequently observed worldwide. Chronic inflammation in the respiratory tract is observed in these diseases. Oxidative stress contributes to this inflammation in the respiratory airways. 8-OHdG is a marker of oxidative stress. The aim of our study was to investigate the 8-OHdG levels in patients with respiratory allergy. Material and Methods: 23 patients with allergy consisting of 11 men and 12 women and 23 healthy controls were enrolled to the study. Results: 8-OHdG levels were found to be higher in allergic patients in comparison to healthy controls but it was not statistically significant. Conclusion: The reason for not significantly high levels of 8-OHdG levels as a marker of oxidative stress in respiratory allergy may attribute to the factor that the inflammation in respiratory allergy is less intensive in comparison to the inflammation in atopic dermatitis.
Biocompatibility of Heparin-Coated Cardiopulmonary Bypass Circuits in Coronary Patients With Left Ventricular Dysfunction Is Superior To Pmea-Coated Circuits
(Wiley, 2006) Kutay, Veysel; Noyan, Tevfik; Ozcan, Sedat; Melek, Yasin; Ekim, Hasan; Yakut, Cevat
Background: Several coating techniques for extracorporeal circulation have been developed to diminish the systemic inflammatory response during cardiopulmonary bypass (CPB). The aim of this study was to evaluate the clinical effectiveness and biocompatibility of heparin-coated and poly-2-methoxyethylacrylate (PMEA)-coated CPB circuits on coronary patients with left ventricular systolic dysfunction. Methods: Thirty-six patients who underwent elective coronary artery bypass grafting were divided into two equal groups: group H (n = 18), heparin-coated; group P (n = 18), PMEA coated. Clinical outcomes, hematologic variables, cardiac enzymes, malondialdehyde (MDA), and acute phase inflammatory response (including myeloperoxidase (MPO), catalase, hsCRP, and IL-8) were analyzed perioperatively. Results: Demographic, CPB, and clinical outcome data were similar for both groups. Plasma fibrinogen, total protein, albumin, and platelet count decreased, neutrophil count, MDA, IL-8, MPO, and catalase levels increased during CPB. During CPB, MPO and catalase values were significantly higher in group P (p = 0.02 and p = 0.01) and postoperative MDA concentration was lower in group H (p = 0.03). Platelet counts were better preserved in group H during and after CPB but neutrophil count and IL-8 level did not differ between the groups. Postoperative total protein, albumin, and fibrinogen levels were higher in group H (p < 0.05). The postoperative first day levels of troponin-I, CK-MB, and CRP increased in both groups without any significant differences between the groups. Conclusions: Heparin-coated circuit provided better suppression of perioperative inflammatory markers and exhibited more favorable effects on hematologic variables than PMEA-coated circuit.
Böbrek Hasarı Markerlerinin Değerlendirilmesinde 24 Saatlik ve Spot İdrar Kullanımı Arasında Fark Var Mıdır
(2001) Şekeroğlu, M. Ramazan; Dülger, Haluk; Noyan, Tevfik
Bu çalışmada, böbrek hasarı markerlerinden, N-asetil-beta-D-glukozaminidaz (NAG), mikroalbumin(MAU) ve beta-2 mikroglobulin (b2M) 'in 24 saatlik ve spot idrar değerleri arasındaki ilişki araştırılmıştır. Bu amaçla, 30 tip 2 diyabetik hastadan 24 saatlik idrar ve ayrıca spot idrar numuneleri toplanmış ve bu numunelerde NAG, b2M ve MAU değerleri ölçülmüştür. Daha sonra 24 saatlik ve spot idrar değerleri arasındaki ilişki araştırılmıştır. Yapılan değerlendirmede, 24 saatlik NAG ile spot NAG ve 24 saatlik MAU ile spot MAU değerleri arasında anlamlı korelasyon bulunurken (sırasıyla, r = 0,403; p=0,027; r = 0,94; p = 0,001), 24 saatlik b2M ile spot b2M arasında anlamlı korelasyon gözlemlenmemiştir (r=0,137; p = 0,472). Bu çalışmanın sonuçları böbrek hasarı markerlerinden NAG ve MAU'nün daha pratik olması açısından 24 saatlik idrar yerine spot idrarda da ölçülebileceğini, ancak b2M'in 24 saatlik ve spot idrar sonuçlarının birbiriyle uyumlu olmadığını göstermiştir.
Determination of Difference Between Groups in Multivariate Analysis of Variance: an Application
(Ortadogu Ad Pres & Publ Co, 2007) Keskin, Siddik; Ankarali, Handan; Noyan, Tevfik; Kamaci, Mansur
Objective: Although there is a healthy trend toward employment of multivariate analysis of variance (MANOVA) in the analysis of data from experiments which involve multiple dependent variables, there remains a need to demonstrate the wide variety of data analytic techniques available for post hoc analysis of statistically significant multivariate results. As a post-hoc test, using Stepdown Analysis after MANOVA was explained with application in this study, Material and Methods: In this study, the data set were used that contain 19 healthy (control group) pregnant, 21 pre-eclamptic pregnant and I I eclamptic pregnant, who submitted to Obstetric and Gynecologist Department of Research Hospital of Y.Y. University Medical School between 2004-2005 years. Urinary protein, systolic blood pressure, lactate dehydrogenase, alanine amino transferase, aspartat transaminase, malonyl dialdehyde and. platelets measurements were measured from pregnants. Results: It showed that urinary protein, systolic blood pressure and LDH were affected on groups' differences according to results of Stepdown analysis. Conclusion: Stepdown analysis, a test for the incremental discriminatory power of a dependent variable after the effects of other dependent variables has been taken into account. This analysis is similar to stepwise regression or discriminant analysis in that it relies on a specific order of entry to determine how much an additional dependent variable adds to the explanation of the differences between the groups in the MANOVA analysis. Because of this properties, Stepdown analysis is a suitable post-hoc tes for MANOVA.
Health From Grape by Resveratrol: Review
(Ortadogu Ad Pres & Publ Co, 2009) Keskin, Nurhan; Noyan, Tevfik; Kunter, Birhan
Resveratrol (3, 4', 5-trihydroxy-stilbene) is a phytoalexin of the stilbene group and is synthezed by grapevine species against biotic and abiotic stress conditions. Especially, it is synthezed in high concentrations in the shell of colored grape cultivars (0.30-0.14 mg/g fw; 9.30-78.50 mg/g dwt). After the discovery of its biochemical structure, resveratrol has been used in the prevention and the treatment of several diseases. Interest on this compound increased after finding its existence in grape and wine. Many medical and pharmacy literatures have emphasized that resveratrol has antifungal, antimicrobial, antitumor and antioxidant effects. Most of the studies about resveratrols are focused on its protective and inhibitory effects on cancer. Resveratrol decreases the risk of coronary heart attack by inhibiting platelet aggregation, reducing cholesterol level and displaying anti-inflammatory activity. In France, low mortality rate from coronary heart diseases depend on moderate amount of wine consumption (French paradox). In addition, recent studies stated that resveratrol had curative effects for Alzheimer's disease. Because it is resistant to heat, resveratrol preserves its own active form in foods. It is easily digested after ingestion and diffuses into blood rapidly. For the protective effect of resveratrol, consumption of 375 mL red wine or 50 grape berries a day or taking resveratrol extract supplements may be recommended. In this review, the protective effect of resveratrol, a protector for grapewine against stress factors, on several diseases of humans such as cancer, heart attack, etc. were discussed with the review of recent literatures.
İleri Evre Gastrik Adenokarsinomalı Olguların Serum ve Mide Sıvısında Vitamin B12, Folik Asit ve Vitamin C Düzeyleri
(2005) Tuncer, İlyas; Uygan, İsmail; Türkdoğan, M. Kürşat; Mercan, Ridvan; Balahoroğlu, Ragıp; Noyan, Tevfik
Amaç: Gastrik kanserin etiyolojisinde diyet faktörleri önemli rol oynamaktadır. Gastrik dokuda antioksidan vitaminlerin düzeylerinin azalması, gastrik malignite riskini artırmaktadır. Çalışmamızda gastrik kanserli olguların serum ve mide sıvısında vitamin B12, folik asit ve vitamin C düzeylerini araştırdık. Materyal ve metod: Endoskopik ve histopatolojik olarak gastrik adenokarsinoma tanısı almış 51 olgu (16 K, 35 E, yaş ort: 56,l p 1,7), kontrol grubu olarak aktif kronik gastrit tanısı almış 44 olgu (18 K, 26 E, yaş ort: 52,8p 2,6) çalışmaya alındı. Kanserli olguların tümü ileri evre mide kanseri idi. Bulgular: Gastrik adenokarsinomada ortalama serum folik asit ve vitamin C konsantrasyonları, kontrol grubu ile karşılaştırıldığında anlamlı olarak düşük bulundu (6,4 p 0,9 vs 9,5 p 1,2) ve (0,8 p 0,1 vs 1,5 p 0,2) (p<0.05). Serumda ortalama vitamin B12 düzeyleri yönünden gruplar arasında istatistiksel anlamlı fark tespit edilmedi. Gastrik sıvıda ortalama folik asit düzeyleri kanserli grupta, kontrol grubuna göre anlamlı olarak yüksek bulunmasına karşın (27,4 p 2,5 vs 19,9 p 1,6) (p<0.05), vitamin B12 ve vitamin C düzeyleri yönünden gruplar arasında anlamlı farklar saptanmadı. Sonuç: Gastrik kanserli olgularda serum folik asit ve vitamin C düzeyleri kontrol grubuna göre düşük bulundu. Oysa mide sıvısında folik asit düzeyleri kontrol grubuna göre anlamlı oranlarda yüksek tespit edildi. Serumda folik asit ve vitamin C değerlerindeki bu düşüklüğün; kanserin oluşmasında birer etiyolojik faktör mü oldukları veya kanser sonrası oluşan malnutrisyona mı sekonder geliştikleri araştırılması gereken konulardır.
The Importance of Oxidative Stress in Patients With Chronic Renal Failure Whose Hypertension Is Treated With Peritoneal Dialysis
(Wiley, 2011) Demirci, Serafettin; Sekeroglu, Mehmet Ramazan; Noyan, Tevfik; Koceroglu, Rusen; Soyoral, Yasemin Usul; Dulger, Haluk; Erkoc, Reha
Increased oxidative stress is a well-known phenomenon in dialysis patients. However, the contribution of hypertension to the oxidative stress in peritoneal dialysis patients has not yet been assessed. The present study aimed to investigate if hypertension had an additional effect on oxidative stress in peritoneal dialysis patients. A total of 50 patients treated with peritoneal dialysis were divided into two groups: The patients with mean of last three blood pressure results as 135/90 mmHg and above were considered hypertensive, the patients with lower blood pressure were considered normotensive. The control group included 25 healthy individuals. Serum malondialdehyde (MDA), advanced oxidation protein product (AOPP), myeloperoxidase (MPO), catalase (CAT) and glutathione peroxidase (GSH-Px) levels were measured in all groups. MDA level, an indicator of lipid peroxidation, was significantly higher in the hypertensive group compared to the control group, while the increase in the normotensive group was not significant. However, the difference between the hypertensive and normotensive groups was significant. The levels of AOPP, an indicator of protein oxidation level, and MPO, an indicator of neutrophil activation, were not different between the groups, while the activities of antioxidant CAT and GSH-Px decreased in both normotensive and hypertensive groups compared to the control group, and there was no significant difference between the patient groups. This study shows that both normotensive and hypertensive peritoneal dialysis patients have increased-oxidative stress and decreased antioxidant levels and hypertension might have an additional effect on oxidative stress by increasing MDA level in peritoneal dialysis patients. Copyright (C) 2011 John Wiley & Sons, Ltd.
Investigation of Changes in Oxidative Stress Before and After Nasal Packing Following Nasal Septoplasty
(Ortadogu Ad Pres & Publ Co, 2008) Noyan, Tevfik; Kiroglu, A. Faruk; Kara, Tolga; Keskin, Siddik
Objective: The aim of the study was to investigate the changes of the oxidant and antioxidant system in blood samples before and after nasal packing following nasal septoplasty. Material and Methods: A total of 23 patients, 7 females and 16 males, who were planned to undergo nasal septoplasty were included in the study. Nasal packing was removed at postoperative 48 hours in all patients. Blood gases, serum advanced oxidation protein products (AOPP), serum and erythrocyte malondialdehyde (MDA) levels, and catalase (CAT) activity were measured at preoperative 24 hours (period 1), postoperative 24 hours (period 2) and on postoperative day 15 (period 3) in all subjects. Results: pO2, pCO2, 02 saturation and HCO3 values were similar at all three periods. MDA level was lower and CAT activity in erythrocyte was higher at period 3 compared to periods 1 and 2. Serum CAT activity was also increased at period 3 compared to period 1. Serum MDA and AOPP levels did not change among the periods. Conclusion: The findings suggest that a decrease in oxidative stress as indicated. by the decrease in lipid peroxidation and increased CAT activity may have a protective role via free radical-scavenging properties 15 days after the operation.
The Investigation of Relationship Between Secondary Hyperparathyroidism and Oxidative Stress in Patients With Chronic Kidney Disease
(Turk Nefroloji Diyaliz Transplantasyon dergisi, 2009) Noyan, Tevfik; Avci, Gulendam; Sekeroglu, M. Ramazan; Erkoc, Reha
OBJECTIVES: Our aim was to investigate the changes of the oxidant and antioxidant systems in chronic kidney disease patients who had increased parathyroid hormone (PTH) levels in the present study. PATIENTS and METHODS: A total of fifty hemodialysis patients were divided into two equal groups based on the PTH levels; > 300 pg/mL (Group 1) and < 300 pg/mL (Group 2). A total 20 healthy subjects were included in the study as the control group (Group 3). The measurement of malondialdehyde (MDA), advanced protein oxidation products level (AOPP), ascorbic acid (AA) levels, and activities of myeloperoxidase (MPO) and catalase (CAT) were performed by colorimetric methods. PTH measurement was also performed by chemiluminescent method. RESULTS: As compared with control group, MDA level was significantly higher in Groups 1 and 2 (p<0.05), and the AOPP level was higher in Group 2 (p<0.05) only. The MPO activity was significantly lower in Group 1 as compared to the control group (p<0.05). The CAT activity and AA levels were significantly lower in Groups 1 and 2 than in the control group (p<0.05). CONCLUSIONS: This study has shown that patients with chronic kidney disease are characterized by increased oxidative stress and decreased antioxidant defense systems. However, increased PTH levels did not have an additional effect on the oxidant and antioxidant mechanisms in these patients.
Kalp Yetmezliği ile Serum Leptin Düzeyi Arasındaki İlişki
(2003) Şekeroğlu, M. Ramazan; Dülger, Haluk; Yalçınkaya, Ahmet; Eryonucu, Beyhan; Akpolat, Tuba; Noyan, Tevfik
Amaç: Vücudun enerji depolan ile ilgili bilgiyi beyne ulaştıran ve obesite geninin ürünü olan leptin hipotalamusu etkileyen protein yapıda bir hormondur. Leptin reseptörleri, aralarında kalp kası da bulunan birçok dokuda bulunmaktadır. Son zamanlarda leptinin kalp yetmezliği ile ilişkisini gösteren çeşitli çalışmalar yapılmıştır. Çalışmamızda serum leptin düzeyi ve kalp hızı değişkenliği (KHD) parametreleri ile olan ilişkilerinin incelenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmaya New York Heart Association sınıflamasına göre 4 farklı evreden 10'ar hasta olmak üzere toplam 40 hasta alınmıştır. Ayrıca bu hasta gruplarıyla karşılaştırmak amacıyla 10 adet sağlıklı bireyden oluşan kontrol grubu oluşturulmuştur. 24 saatlik holter kayıtlarından elde edilen KHD'nin zaman-alan parametreleri saptanmış ve leptin düzeyi ile olan ilişkileri değerlendirilmiştir. Bulgular: Leptin düzeylerinin evre III ve IV kalp yetmezliği gruplarında evre I'e göre anlamlı olarak (sırasıyla p<0.05 ve p<0.01) azaldığı saptanmıştır. Ayrıca KHD parametreleri ile leptin düzeyi arasında pozitif korelasyon gözlenmiştir. Sonuç: Kalp yetmezliğinin ileri evrelerinde serum leptin düzeyleri azalmış olup, bunun da artan sempatik aktivite ile ilişkili olabileceği düşünülmüştür.
Kanserli Hastalarda Kemoterapinin Lipid Peroksidasyonu Üzerine Etkisi
(2002) Dülger, Haluk; Alıcı, Süleyman; Noyan, Tevfik; Şekeroğlu, M. Ramazan; Yalçınkaya, Ahmet
Serbest radikaller ve bu radikallerin etkisiyle oluşan lipid peroksitlerinin pek çok hastalığın patogenezinde rol oynadığı bilinmektedir. Bu çalışmada kanser tanısı konmuş ve önceden kemoterapi ve radyoterapi almamış 21 hastada tedavi öncesi ve ikinci kemoterapi siklüsü sonrası olmak üzere lipid peroksidasyon Ürünlerinden serum malonildialdehit (MDA), glukoz, t. protein ve trigliserid düzeyleri araştırılarak kontrol grubu serum MDA, glukoz, t. protein ve trigliserid düzeyleri ile karşılaştırıldı. Hasta ve kontrol gruplarının 10'u erkek, 11'i kadındı. Yaş ortalaması (yıl olarak) hasta grubunda 52.61±15.68, kontrol grubunda ise 47.00±4.37 idi. Hastaların %39'u gastrointestinal sistem tümörü, %34'ü meme tümörü ve %27'si diğer tümörlere sahipti. Hasta grubunda tedavi öncesi ve ikinci siklüs kemoterapi sonrası ortalama MDA düzeyleri sırasıyla 9.01±2.52 nmol/ml ve 8.90±2.74 nmol/ml idi. Kontrol grubunun ortalama MDA düzeyi 3.94±1.41 nmol/ml idi. Hasta grubunda tedavi öncesi ve ikinci siklüs kemoterapi sonrası MDA düzeyleri karşılaştırıldığında istatistiksel olarak anlamlı fark yoktu (p>0.05). Kontrol grubu ile hasta grubu MDA düzeyleri istatiksel olarak karşılaştırıldığında anlamlı fark saptandı (p<0.001). Bu çalışma kanserli hastalarda serum MDA düzeylerinin sağlıklı bireylere göre belirgin olarak yüksek olduğunu, ancak iki kemoterapi seansı sonunda serum MDA düzeyinde anlamlı bir değişikliğin olmadığını göstermiştir.
Serum Advanced Oxidation Protein Products, Myeloperoxidase and Ascorbic Acid in Pre-Eclampsia and Eclampsia
(Wiley, 2006) Noyan, Tevfik; Guler, Ayse; Sekeroglu, Mehmet Ramazan; Kamaci, Mansur
Background: Activation products from neutrophils and the complement system might cause endothelial dysfunction, which is central to the aetiology of pre-eclampsia. This study aimed to investigate the activity of myeloperoxidase (MPO), and its association with advanced oxidation protein products (AOPP), in women with pre-eclampsia and eclampsia. Materials and method: Twenty-one pregnant women with pre-eclampsia, 11 pregnant women with eclampsia and 19 healthy pregnant women were studied. Serum levels of malondialdehyde (MDA), AOPP, ascorbic acid (AA) and activities of MPO and catalase (CAT) were measured using a colorimetric method. Results: The MDA level was significantly higher in the pre-eclampsia (3.15 +/- 0.28 nmol/mL) and eclampsia (4.01 +/- 0.66 nmol/mL) groups than in controls (1.85 +/- 0.18 nmol/mL); the difference between MDA levels in the pre-eclampsia and eclampsia groups was not statistically significant. MPO activity was significantly higher in the eclampsia (347.59 +/- 88.06 U/L) group than in the pre-eclampsia (196.17 +/- 30.8) and control (93.22 +/- 9.52) groups, and there was also no significant difference in these levels between the pre-eclampsia and control groups. CAT activity was significantly higher in the pre-eclampsia (166.35 +/- 31.75 U/L) and eclampsia (166.98 +/- 40.31 U/L) groups than in controls (81.28 +/- 7.41 U/L), and AA level was significantly higher in the pre-eclampsia (0.54 +/- 0.15 mg/dL) group than in controls (0.18 +/- 0.01 mg/dL); the differences in AA and CAT activity between the pre-eclampsia and eclampsia groups were not statistically significant. AOPP levels did not change significantly among the control, pre-eclampsia and eclampsia groups (106.88 +/- 5.62, 98.89 +/- 6.47, 111.89 +/- 6.8 mu mol/L, respectively). Conclusions: We suggest that increased oxidative stress might contribute to the pathophysiological mechanisms of pre-eclampsia and eclampsia, and that AA and CAT might have a protective role via free radical-scavenging properties. However, further study is needed.
Serum Cytokines and Bone Metabolism in Patients With Thyroid Dysfunction
(Health Communications inc, 2006) Sekeroglu, M. Ramazan; Altun, Z. Busra; Algun, Ekrem; Dulger, Haluk; Noyan, Tevfik; Balaharoglu, Ragip; Ozturk, Mustafa
Hyperthyroidism is associated with increased bone turnover. Besides the hormones of calcium metabolism , locally produced factors are important in maintaining normal bone metabolism. Interleukin-6 (IL-6), in particular, has a major influence on bone turnover. In this study, serum IL-6 and tumor necrosis factor-alpha (TNF-alpha) levels, as well as bone turnover markers and relationships between them, were investigated in hyperthyroidism and hypothyroidism. A total of 20 female patients with hyperthyroidism, 15 with subclinical hyperthyroidism, 16 with hypothyroidism, and 15 with subclinical hypothyroidism constituted the patient groups. In all, 15 age-matched healthy female volunteers were recruited as controls. When compared with controls, serum TNF-a levels showed no significant difference in any of the patient groups (P >.05). In the groups with hyperthyroidism and subclinical hyperthyroidism, IL-6 levels were significantly higher compared with control group values (P <.05). Hyperthyroid patients showed higher levels of alkaline phosphatase (ALP) and osteocalcin, and a higher urinary deoxypyridinoline/creatinine ratio, compared with controls (P <.05). In subclinical hyperthyroidism, only ALP was found to be higher compared with control values. No significant correlations were made in any group between serum IL-6 or TNF-a level and bone turnover markers. Results suggest that serum IL-6 level and markers of bone turnover rate seem to be increased in hyperthyroidism. This finding may support the role of IL-6 in induction of bone turnover in hyperthyroid states.
The Serum Interleukin-6 and Tumor Necrosis Factor-Α Levels and Their Relationship With Antithrombin-Iii and Von Willebrand Factor in Preeclampsia
(Galenos Yayincilik, 2006) Noyan, Tevfik; Bursal, Ercan; Sekeroglu, Mehmet Ramazan; Dulger, Haluk; Kamaci, Mansur
Objective: Preeclampsia, characterized by changes in the placenta and uteroplacental vasculature, is the most frequent complication of pregnancy. It is argued that an overproduction of placental cytokines may be associated with the pathophysiological changes found in preeclampsia. In this study, it is aimed to determine the levels of both pro-inflammatory (tumor necrosis factor-alpha) and immunoregulatory (interleukin-6) cytokines and their relationships with von Willebrand factor and anti-thrombin III from both preeclamptic and healthy pregnant women. Material and Methods: Twenty-five pregnant women with preeclampsia and 25 healthy pregnant women were included in the study. The fasting blood samples were obtained at eight o'clock in the morning from the both groups and interleukin-6, tumor necrosis factor-alpha, von Willebrand factor and anti-thrombin III levels were measured. From the preeclamptic patients blood samples were obtained at twelve o'clock midnight to determine the diurnal variation in these two cytokine levels. Results: As compared to healthy pregnant group, levels of interleukin-6 and anti-thrombin III were significantly lower and von Willebrand factor level was significantly higher in the preeclamptic group (p< 0.05). Tumor necrosis factor-alpha level did not differ significantly between the two groups. Interleukin-6 and tumor necrosis factor-alpha levels did not show significant diurnal variation in preeclamptic group. A significant positive correlation was found between the tumor necrosis factor-alpha levels measured in the morning and night samples in the preeclamptic group (p< 0.01, r=0.701). Also, a significant positive correlation was found between the tumor necrosis factor-alpha and von Willebrand factor levels in healthy pregnant group (p< 0.05, r=0.648). Conclusions: These findings suggest that preeclampsia is associated with decreased interleukin-6, and anti-thrombin III, and with increased von Willebrand factor levels.
Serum Mast Cell Tryptase, Eosinophil Cationic Protein, Endothelin-1 and Cytokine Levels in Preeclampsia and Healthy Pregnancy
(Walter de Gruyter Gmbh, 2009) Noyan, Tevfik; Kolusari, Ali; Kamaci, Mansur
Aim: Preeclampsia is characterized by both local and systemic changes in cytokine balance and endothelial dysfunction. It was aimed to investigate the possible role of cytokines, endothelin and mast cell in the pathophysiology of preeclampsia in this study. Material and Methods: Twenty five pregnant women with preeclampsia (group 1), 25 healthy pregnant women (group 2) and 25 healthy non-pregnant women as a control (group 3) were included in the study. The soluble interleukin-2 receptor, interleukin-10, tumor necrosis factor alpha and eosinophil cationic protein were measured by using a non-radioimmunoassay chemiluminescent method, endothelin-1 and myeloperoxidase by using enzyme-linked immunosorbent method and mast cell tryptase by using fluorescent enzyme immunoassay method. Results: The myeloperoxidase activity was significantly higher in group 1 than in groups 2 and 3 (p<0.05). Mast cell tryptase level was significantly lower in groups 1 and 2 than in group 3 (p<0.05). Soluble interleukin-2 receptor level was significantly higher in group 2 than in groups 1 and 3 (p<0.05). Tumor necrosis factor alpha level was significantly higher in groups 1 and 2 than in group 3 (p<0.05). Endothelin-1, interleukin-10 and eosinophil cationic protein levels did not change significantly among the groups. Conclusion: The result of this study indicated that mast cell was restrained in both preeclampsia and healthy pregnancy. The inflammatory changes might also contribute to the pathogenesis of preeclampsia.
A Study of Oxidative Stress Parameters in Anti-Helicobacter Pylorus Immunoglobulin G Positive and Negative Gastric Cancer Patients
(Yonsei Univ Coll Medicine, 2009) Noyan, Tevfik; Guducuoglu, Hueseyin; Ilhan, Mahmut
Purpose: Helicobacter pylorurs (HP) is a Gram-negative spiral-shaped microaerophitic bacterium, which colonizes in the gastric mucosa of humans. The gastric human pathogen HP causes chronic gastritis and ulcers, and has a strong relationship with gastric cancer. The aim of this study was to determine advanced oxidation protein products (AOPP) levels, activities of myeloperoxidase (MPO) and catalase (CAT) in two groups. Materials and Methods: For this aim, one group included 30 patients with gastric cancer (Group 1) and the other included 30 subjects with non-gastric cancer and Anti-HP immunoglobulin (Ig) G antibody positive (group 2). Anti-HP IgG antibody test values were found as positive in fifty percent of group I and all of the group 2 patients. Results: Significantly increased AOOP levels were found in group 1 (p < 0.05) compared to group 2. There were no significant differences between the groups in regard to activities of M PO and CAT. In addition, AOPP level, MPO and CAT activities were similar among the Anti-HP IgG positive and negative subgroups of group 1 patients. Conclusion: The result of this study indicated that gastric cancer patients were characterized by increased protein oxidation, whereas there was no significant difference in oxidative stress parameters and antioxidant enzyme activity between the Anti-HP IgG positive and negative gastric cancer patients.
The Importance of Oxidative Stres at Chronic Kidney Failure Patients Have Seconder Hyperparathroidsm Treated With Hemodialysis
(2007) Avcı, Gülendam; Noyan, Tevfik
Kronik böbrek yetmezliği (KBY); glomerüler filtrasyon değerinin azalmasına bağlı olarak, böbrek fonksiyon bozukluğuyla kendini gösteren, kronik ve ilerleyici bir hastalıktır. Oksidatif stres ile KBY'nin ilerlemesi arasında bir ilişki olduğu bildirilmektedir. Bununla birlikte, sekonder hiperparatroidizm kronik hemodiyaliz hastalarında en önemli komplikasyonlardan biridir. Bu çalışma; paratroid hormon (PTH) seviyeleri yüksek olan hemodiyaliz hastalarında, PTH yüksekliğinin oksidatif stres ve antioksidan sistemlerde nasıl bir değişiklik yaptığının belirlenması amacıyla yapılmıştır. Bu amaçla; hemodiyalizle tedavi edilen toplam 50 hasta; PTH seviyesi 300 pg/mL üstünde (Grup 1) ve altında (Grup 2) olmak üzere iki gruba ayrıldı. Kontrol grubu (grup 3) olarak ise böbrek fonksiyonları ve PTH seviyeleri normal değerler arasında olan 20 sağlıklı birey alındı. Çalışmada; lipid peroksidasyonunun bir göstergesi olan malondialdehit (MDA) seviyesi, 1. ve 2. grupta kontrol grubuna göre anlamlı (p<0.05) olarak yüksek bulunurken, protein oksidasyonunun göstergesi olan ileri oksidasyon protein ürünü (AOPP) seviyesi, yalnızca 2. grupta kontrol grubuna göre anlamlı olarak yüksek bulunmuştur (p<0.05). Nötrofil aktivasyonunun göstergesi olan myeloperoksidaz (MPO) aktivitesi; 1.grupta kontrol grubuna göre anlamlı olarak düşük bulunmuştur (p<0.05). Antioksidan etki gösteren katalaz (KAT) aktivitesi ve C vitamini seviyesi ise hem grup 1'de hem de grup 2'de kontrol grubuna göre anlamlı olarak düşük bulunmuştur (p<0.05). Bununla birlikte, KAT aktivitesi; 1.grupta, 2. gruptan daha düşük bulunmuştur (p<0.05). Bu çalışmanın sonuçları, hemodiyaliz hastalarının artmış oksidatif stres ve azalmış antioksidan etkiye sahip olduğunu göstermektedir. Yine, PTH yüksekliğinin, hemodiyaliz hastalarında oksidatif stresin artışında ek bir etkiye sahip olmadığını da göstermektedir. Anahtar Sözcükler: Antioksidan savunma, kronik böbrek yetmezliği, oksidatif stres, sekonder hiperparatrodizm
Tip 1 Diabetli Hastalarda Antitiroid Antikor Düzeyleri
(2003) Dülger, Haluk; Yalçınkaya, A. Sadık; Balaharoğlu, Ragıp; Noyan, Tevfik; Şekeroğlu, M. Ramazan; Algün, Ekrem; Ayakta, Hayati
Bu çalışma, tip 1 diabetik hastalarda antitiroid antikorlardan anti-tiroglobulin antikor (anti-TG ab) ve anti-peroksidaz antikor (anti-TPO ab) düzeylerini araştırmak amacıyla yapıldı. Bu amaçla, Tip 1 diabetli glisemik kontrol altındaki 20 hasta (12'si kadın, 8'i erkek) çalışma grubu olarak alındı. Bu hastalarla karşılaştırma yapmak amacıyla Tip 2 diabetli 20 hasta (14'ü kadın, 6'sı erkek) ile 20 sağlıklı birey (15'i kadın, 5 'i erkek) kontrol grubu olarak alındı. Her üç grupta da serum glukoz, T3, T4, TSH, FT3, FT4, anti-Tg ab, anti-TPO ab ölçümleri yapıldı. Kontrol ve hasta gruplarına ait bulgular istatistiksel olarak karşılaştırıldığında her üç grup arasında serum T3, T4 ve FT4'te istatistiksel açıdan önemli bir fark tespit edilemedi. Tip 1 diabet grubunun anti-Tg ab ve anti-TPO ab seviyeleri, kontrol grubuna ve tip 2 diyabet grubuna göre anlamlı derecede yüksek bulunurken(p<0.05); tip 2 diabet grubunun anti-Tg ab ve anti-TPO ab seviyeleri kontrol grubuna göre yüksek bulunmuş fakat istatistiksel açıdan anlamlı bulunmamıştır. Yine tip 1 diabet grubunun TSH seviyeleri, kontrol grubu ve tip 2 diabet grubuna göre anlamlı derecede yüksek bulunurken (p<0.05); tip 1 diabet FT3 seviyeleri kontrol grubu ve tip 2 diabete göre anlamlı derecede düşük bulunmuştur(p<0.05). Tip 1 diabetik grupta anti-Tg ab sıklığı % 25, anti-TPO ab sıklığı ise % 45 olarak bulunmuştur. Bulgularımızdan, Tip I diabetik hastalarda tiroid otoantikor prevalansının ve hipotiroidi riskinin arttığı görülmüştür. Tip 1 diyabetik hastalarda yüksek serum anti-Tg ab ve anti-TPO ab'na sahip hastaların tiroid fonksiyonlarının yakından takip edilmesinin uygun olacağı kanısındayız.
Tubular Function in Pregnant Women With Preeclampsia
(2004) Kamacı, Mansur; Şekeroğlu, M. Ramazan; Noyan, Tevfik; Dülger, Haluk
Amaç: Preeklampsi (PE) gebeliğin sık oluşan komplikasyonlarından biridir. PE, proteinüri ve glomerüler filtrasyonda azalmayla karakterize böbrek tutulumuna sıklıkla sahiptir. Bu çalışmanın amacı, preeklamptik ve normal gebelerde, gebe olmayan sağlıklı kadınlarla karşılaştırıldığında tubuler fonksiyonun göstergesi olan beta-2 mikroglobulin (b2 M)in idrar ve fraksiyonel atılımındaki değişimleri araştırmaktır. Gereç ve Yöntemler: Bu çalışmaya, üçüncü trimesterde olan 20 preeklamptik (grup 1), 20 sağlıklı gebe (grup 2) ve kontrol grubu olarak da gebe olmayan 20 sağlıklı kadın (grup 3) katıldı. Her bir gruptaki kadınlarda serum ve idrar beta-2 mikroglobulin düzeyi ölçülerek, b2 M fraksiyonel eksresyonu hesaplandı. İdrar b2 M değeri, idrar kreatinin değerine oranlanarak verildi (b2 M/Cr). Bulgular: İdrar b2 M/Cr oranı grup 3le karşılaştırıldığında, grup 1 ve 2de anlamlı olarak yüksekti (p<0.001), fakat grup 1 ve 2 arasında anlamlı bir farklılık yoktu (p>0.05). Grup 2 ve 3le karşılaştırıldığında grup 1de serum b2 M değerleri anlamlı olarak yüksekti (p<0.01) ve grup 2 ve 3 arasında anlamlı bir farklılık yoktu (p>0.05). Grup 3le karşılaştırıldığında grup 1 ve 2de b2 M in fraksiyonel eksresyonu anlamlı olarak artmıştı (p<0.05) ve grup 1 ve 2 arasında anlamlı bir farklılık yoktu (p>0.05). Sonuç: Çalışmamızın sonuçları, preeklampsili ve normal sağlıklı gebelerde, gebe olmayan sağlıklı kadınlara göre b2 Min idrar ve fraksiyonel atılımının önemli oranda arttığını ve bu artışın tubuler fonksiyon bozukluğunun bir belirtisi olabileceğini göstermektedir.