Browsing by Author "Oğuz, B."

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Current Status of Zoonotic Pathogen Anaplasma Phagocytophilum in Türkiye: a Molecular Approach
(Aras Part Medical International Press, 2021) Oğuz, B.; Değer, M.S.
Cytochrome Oxidase I Gene Sequences That Cause Hypodermosis Cattle Species by PCR-RFLP Technique Investigation
(2013) Oğuz, B.
Evaluation of Oxidant/Antioxidant Markers, Cytokines, DNA Damage and Trace Mineral Levels in Goats Naturally Single and Co-Infected with Theileria Ovis and Anaplasma Ovis
(Alexandria University, Faculty of Veterinary Medicine, 2024) Mi̇s, L.; Deǧer, Y.; Oğuz, B.
The impact of co-infections with Theileria ovis and Anaplasma ovis on caprine health is very limited knowledge and is rarely documented in the literature. Türkiye is endemic for tick-borne diseases (TBDs), posing a risk of co-infections in goats. To evaluate the impact of single and co-infection with T. ovis and A. ovis on caprine health, four groups of goats were examined: healthy goats, goats infected with T. ovis, goats infected with A. ovis and goats co-infected with both species. Sera from blood samples was tested for oxidant/antioxidant markers [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST)], cytokines [interleukins (IL)-6, IL-1, tumor necrosis factor-α (TNF-α)], DNA damage [8-hydroxy-2'-deoxyguanosine (8-OHdG)] and trace minerals [(copper (Cu), zinc (Zn), manganese (Mn), selenium (Se)]. MDA, 8-OHdG, TNF-α, IL-1 and IL-6 levels, CAT and GST enzyme activities were found to be significantly increased in both Theileria ovis and Anaplasma ovis single- and co-infections (P<0.05). In goats, single and co-infected with A. ovis showed a statistically significant increase in SOD enzyme activity compared to goats infected only with T. ovis (P<0.05). In goats co-infected with A. ovis and T. ovis, considerable decrease in GPx enzyme activity were observed (P<0.05). Zn and Se levels in single infected with A. ovis indicated a significant decrease. Also, Cu, Zn, and Se levels in co-infected animals decreased significantly (P<0.05). Significant increase in lipid peroxidation and antioxidant enzyme activity in goats with single and double infections indicates impaired erythrocyte membrane integrity due to oxidative stress. Moreover, increased proinflammatory cytokine concentrations in all groups indicate that a high degree of systemic inflammation is induced during parasitaemia. © 2024 Alexandria University, Faculty of Veterinary Medicine. All rights reserved.
First Investigation on Vectorial Potential of Blattella Germanica in Turkey
(Chartered Inst. of Building Services Engineers, 2017) Oğuz, B.; Özdal, N.; Orunç Kilinç, Ö.; Değer, M.S.
Cockroaches are claimed to be mechanical vectors of microorganisms such as intestinal parasites, bacteria, fungi, and viruses. This study was conducted to determine the potential role of cockroaches as carriers of parasites having medical importance in Van province, Turkey. One hundred and thirty-eight cockroaches were collected from different parts of apartments and houses between March and April 2014. All of the collected cockroaches were identified as Blatella germanica. They were examined for isolation and identification of intestinal parasites from external surface. The results showed that 66 (48%) of the cockroaches harbored parasitic organisms. Of these, 96.6% were protozoon and the remaining 3.4% were helminthes. Isolated helminth, species were Toxocara sp. (3%), Ascaris lumbricoides (3%), Trichostrongylus sp. (1.5%), Trichuris trichiura (1.5%) and unidentified nematode egg samples (3%). The protozoon identified during the study were Endolimax nana (7.6%), Blastocystis hominis (41%), Entamoeba histolytica/E. dispar (16.7%), unsporulated coccidial oocyst (7.6%), Chilomastix mesnilli (4.5%), Entamoeba coli (35%), Giardia sp. (13.6%) and Iodamoeba butschlii (7.6%). In conclusion, Blattella germanica was found to harbor intestinal parasites of public health importance. Hence, awareness on the potential role of cockroaches in the mechanical transmission of intestinal parasites needs to be further investigated. Control of cockroaches will substantially minimize the spread of diseases caused by helminths and protozoons in our environment. © 2017, Chartered Inst. of Building Services Engineers. All rights reserved.
Investigation of Thiol-Disulphide Homeostasis, Total Oxidant-Antioxidant and Ischaemia-Modified Albumin Levels in Dogs With Babesiosis
(Alexandria University, Faculty of Veterinary Medicine, 2023) Tarhan, M.; Deǧer, Y.; Oğuz, B.; Ozdek, U.
This study was designed to reveal the diagnostic efficiency of total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), thiol/disulphide homeostasis (TDH), albumin (ALB) and ischaemia-modified albumin (IMA) parameters in the assessment of oxidative stress in dogs with babesiosis. While the babesiosis group consisted of a total of 7 dogs with clinical and parasitological (ELISA) diagnoses of babesiosis, the control group consisted of 7 healthy dogs. Blood samples were taken from both groups. While the levels of TAC, TOC and ALB in the blood serums of the groups were measured using a commercial kit, the levels of total thiol (TT), native thiol (NT) and IMA were measured using the method. The OSI and disulphide (Ds) levels were calculated using the formula. When compared with the control group, TAC and ALB levels significantly decreased, whereas the levels of TOC and OSI elevated in the babesiosis group (p<0.05). No difference was found between the groups in terms of NT, TT, Ds, and IMA levels and the ratios of NT/TT, Ds/TT, and Ds/NT (p>0.05). A negative significant correlation was found between TAC and OSI and between Ds/NT and NT, TT and NT/TT (p<0.05; p<0.001, respectively). A significant positive correlation was found between NT and TT and NT/TT and between TT and NT/TT (p<0.001). The areas under the ROC curve of the variables TAC, TOC, OSI and ALB were found to be 0.918, 0.796, 0.980 and 1, respectively. The results showed that TAC, TOC, OSI and ALB performed diagnostically in the assessment of elevated oxidative stress in dogs with babesiosis. © 2023 Alexandria University, Faculty of Veterinary Medicine. All rights reserved.
Prevalence of Elisa-Detected Specific Antibodies Against Besnoitia Besnoiti in Cattle of the Eastern and Southeastern Anatolian Regions, Turkey
(Shiraz University, 2019) Özdal, N.; Oğuz, B.; Orunç Kılınç, Ö.; Karakuş, A.; Değer, S.
Background: Besnoitiosis caused by Besnoitia besnoiti is regarded as a re-emerging disease in cattle because of the increased number of cases and geographical distribution in many European countries. Aims: The present study was conducted to determine the presence of B. besnoiti in cattle in the Eastern and Southeastern Anatolia of Turkey. Methods: Blood samples were collected from 450 cattle in the provinces of Mus, Van, Siirt, and Diyarbakir. PrioCHECK®Besnoitia Ab 2.0 enzyme-linked immunosorbent assay (ELISA) kit was used to detect specific anti-B. besnoiti antibodies in the serum samples. Results: Twelve (2.7%) of the 450 asymptomatic cattle were seropositive against B. besnoiti. In cattle, the prevalence rates were 0%, 3.7%, 3.4%, and 1.1% in Mus, Siirt, Diyarbakir, and Van provinces (P>0.05), respectively. This study is the first to investigate the presence of B. besnoiti in cattle raised in the Eastern and Southeastern Anatolia of Turkey. Conclusion: Although the ELISA test revealed some positive cases, concrete evidence for the establishment of clinical B. besnoiti infection could not be verified. More comprehensive analysis would be necessary to determine the significance of the present observations. © 2019 Shiraz University. All rights reserved.