Browsing by Author "Ogun, E."

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Determination of Total Cell Protein Profiles of Streptomyces Species
(Journal Experimental Biology & Agricultural Sciences, 2013) Zdemir, K.; Berber, I; Ogun, E.; Atalan, M.
Present study has been conducted for finding out the total protein profile of bacterial strain Streptomyces sps by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Total 139 isolates of Streptomyces have been isolated from the soil. Amongst all isolated strain, total 20 isolates were used for getting protein profile by SDS PAGE. Amongst all isolates, 20 isolates were selected for protein profiling and these were divided in two groups. Two strains of Streptomyces i.e. S. violaceus and S. albidoflavus were selected as a reference strain for both groups. Band profile were analyzed and assessed by computer added program BioRad Quantity with the use of Unweighted Pair Group Method of Analysis (UPGMA). As a result of this computer assisted numeric analysis study, approximately 40 different types of protein bands were reported between 10 or 100 kD molecular weight. Analysis of acquired dendogram on the basis of similarities ratios, all 40 proteins can be divided in to 7 groups. In addition, the isolates A4B3G, D145B, S5036.6 and reference isolate S. violaceus were available in the same group, while 805A, C804B, F1705 isolates and reference sample S. albidoflavus were detected in the same group. The test organisms which were similar to each other in terms of morphological and biochemical characters delivered the same protein bands. SDS-PAGE method is an effective method in terms of determining taxonomical relations between the various species of genus Streptomyces.
The Effects of Wood Vinegar on Some Soil Microorganisms
(Aloki Applied Ecological Research and Forensic inst Ltd, 2019) Koc, I; Ogun, E.; Namli, A.; Mendes, M.; Kutlu, E.; Yardim, E. N.
This study was carried out in order to determine the enzyme activity of the wood vinegar obtained from the hazelnut shells with the potential of bio-pesticide in agro-ecosystem soil, and its effect on the microfungi and heterotrophic bacteria in total. The study was realized in the production seasons of 2014-2015 and 2015-2016, in the ecological conditions of the province Mus (in Turkey) on winter wheat field and with four repetitions according to Randomized Blocks Experimental Design. The treatments within the scope of the experiment were conducted as the six different doses of wood vinegar at 0.5%, 1.0%, 2.0%, 3.0%, 4.0%, and 5.0% mL, and the control treatment which was only provided with tap water. Repeated Measurement ANOVA was used to determine the effect of wood vinegar at different doses, the production seasons and periods (pre- and post-treatment) on relevant features. As based on the statistical inferences, only the Treatment x Year and Period x Year interaction effects in terms of the number of heterotrophic bacteria (P = 0.000 and P = 0.011); and only the Period x Year interaction effects for the number of microfungi (P = 0.000) were found statistically significant. Therefore, the effect of the treatments made on the number of heterotrophic bacteria varied by the production seasons. In a similar way, the effect of the production season and periods on the number of microfungi also changed as based on the term. While the effect of period (P = 0.000) and the Treatment x Period interaction (P = 0.014) effect were significant for Alkaline Phosphatase activity, Year x Period (P = 0.001) and Treatment x Period interactions (P = 0.000) were found significant as related to Beta Glucosidase activity. In conclusion, it may be stated that the wood vinegar used at different doses with the purpose of protecting plant and/or crop in wheat agro-ecosystem does not have a negative effect on microbial factors determined in the soil, and especially, the treatment of 3% mL has a positive effect on bacteria number and Beta Glucosidase enzyme activity.
Identification of Biodiversity of Some Streptomyces Species and Determination of a Restriction Fragment Length Polymorphism (Rflp) Profile of 16s Rdna Gene Region
(Medwell Journals, 2014) Ozdemir, K.; Ogun, E.; Ertas, M.; Acar, S.; Atalan, E.
In this study, soil samples from the roots and peripheral regions of some agricultural plant species growing in the Van region (such as sainfoin, bean, malus, wheat and sugar beet) were collected between May and June 2004 and 139 strains of Streptomyces bacteria were isolated from these samples. Moreover, researchers determined the physicochemical characteristics of these soil samples. Streptomyces bacteria were color-grouped and the Diaminopimelic Acid (DAP) forms were detected followed by the determination of then phenotypic characteristics. When these isolates were divided into 39 color groups, 20 strains were found to contain L-DAP and therefore were confirmed as members of the genus Streptomyces. The species were identified using the TAXON Software and similarity dendrograms were constructed by the Unweighted Pair-Group Method with an Arithmetic average (UPGMA) cluster analysis using the Multi-Variate Statistical Package (MVSP 3.1). Genomic DNA was isolated from these bacteria and 16S rDNA gene was amplified by PCPv followed by restriction digestion with Bspl43I, Haelll, Mnll and SphI for Restriction Fragment Length Polymorphism (RFLP) analysis. In the dendrogram obtained at the end of the RFLP analysis, four groups of two or more members and six 1 -member groups were established according to the similarity ratio criterion. © Medwell Journals, 2014.