Browsing by Author "Oguz, Ahmet Regaip"

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Neuromuscular Degenerative Effects of Ankaferd Blood Stopper® in Mouse Sciatic Nerve Model
(Taylor & Francis Ltd, 2017) Ustun, Ramazan; Oguz, Elif Kaval; Delilbasi, Cagri; Seker, Ayse; Taspinar, Filiz; Oncu, Mehmet Resit; Oguz, Ahmet Regaip
Purpose: Ankaferd Blood Stopper((R)) (ABS), a licenced medicinal herbal extract, is commonly used as an effective topical haemostatic agent. This study is designed to investigate whether topical ABS application may cause peripheral nerve degeneration and neuromuscular dysfunction in a mouse sciatic nerve model.Methods: Twenty mice were randomly divided into two groups; an ABS treated experimental group and a saline-treated control group. Left sciatic nerves were treated with 0.3ml of ABS in the experimental group and 0.3ml of sterile saline in the control group for 5min. Peripheral nerve degeneration and neuromuscular dysfunction were evaluated by behavioural tests, electrophysiological analysis and weight ratio comparison of target muscles.Results: The motor function, assessed by the sciatic function index, was significantly impaired in ABS-treated animals as compared to the animals treated with saline. Motor coordination, evaluated with the rotarod test, was significantly decreased (-42%) in ABS-treated animals compared to the saline-treated animals. The degree of pain, assessed by the reaction latency to thermal stimuli (hot-plate test), was significantly prolonged (313%) in ABS-treated mice when compared to the saline-treated mice. ABS-treated mice showed a significant reduction in motor nerve conduction velocity (MNCV) (-52%) and the compound muscle action potential (CMAP) (-47%); however, it significantly prolonged onset latency (23%). The gastrocnemius muscles weight ratio of the ABS group was considerably lower than that of the control group.Conclusions: These findings demonstrate that ABS triggers peripheral nerve degeneration and functional impairment and, thus promotes a deterioration of sciatic nerves.
Protective Effects of Chitosan and Chitosan Oligosaccharide Against Oxidative Damage in Liver Tissue of Rats With Fluorine Poisoning
(Univ Karachi, 2021) Ozdek, Ugur; Komuroglu, Ahmet Ufuk; Oguz, Ahmet Regaip; Deger, Yeter
Fluorine toxicity has negative effects on soft tissue besides skeletal and dental tissues. In the present study, we have investigated the protective effect of chitosan (CS) and chitosan oligosaccharide (COS) on liver tissue of fluorine-intoxicated rats taking the antioxidant characteristics of chitosan and its derivatives into consideration. In this study, 42 male Wistar albino rats were randomly selected to determine the control and experimental fluorosis groups. Our study lasted for 12 weeks. As a consequence of the study, MDA significantly increased in the liver tissue of NaF group while some antioxidant values significantly decreased. It was detected that serum AST and LDH levels increased significantly while ALB and TP values significantly decreased in NaF group. The degenerations were identified in the liver histopathology of all fluoride- treated groups. We have concluded according to the results that chitosan oligosaccharide can be more effective compared with chitosan.
The Variations in The Levels of Some Metals in The Different Tissues of Van Fish (Alburnus Tarichi, Guldenstadt 1814) According To Gender and Weight
(Parlar Scientific Publications (p S P), 2017) Yeltekin, Asli Cilingir; Oguz, Ahmet Regaip
Environmental pollution threatens the ecosystem and people more and more every day. This situation is also valid for Lake Van. For this reason, the alterations in the levels of some metals were studied according to gender and weight in Van fish (Alburnus Tarichi, Giildenstadt 1814). In the study, beryllium (Be), bismuth (Bi), lead (Pb), cadmium (Cd), iron (Fe), copper (Cu), zinc (Zn), selenium (Se), nickel (Ni) and manganese (Mn) elements in the tissues of muscles, liver, gills, gonads and brain of Van fish were analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES). The results were evaluated as toxic elements and trace elements. Highly toxic Be, Bi, Pb and Cd elements were found in the tissues of Van fish. Especially in the brain tissues, their concentrations were observed to be higher. Ni metal's tissue concentration in the brain was found to be higher than the other tissues. In the study, the metal concentrations in the male fish were found to be higher than the female fish (p<0.05). According to their weights, the levels were found to be higher in the 80>Weight group and 80-100 Weight group, and lower in the 100