Browsing by Author "Oguz, B."

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A Case Report of Echinococcus Granulosus Sensu Stricto (G1) in a Domestic Cat in Turkey
(Hellenic veterinary Medical Soc, 2021) Oguz, B.; Selcin, O.; Deger, M. S.; Bicek, K.; Ozdal, N.
Echinococcus granulosus sensu lato is a zoonotic helminth with a life cycle that include of definitive hosts (dogs and wild carnivores) and intermediate hosts (usually the even-toed ungulates, Artiodactyla). Intermediate hosts become infected by ingesting the parasite eggs in contaminated food and water.Accidental intermediate hosts acquire infection in a similar way as other intermediate hosts. A two-year-old female cat was presented to the Van (Turkey) Animal Care and Rehabilitation Center with abdominal tension. Multiple intraperitoneal vesicles, which were found to be E. granulosus (s.1.) metacestodes, were observed during the ultrasound imaging. Then, the animal was laparotomized. Phylogenetic analysis based the partial cytochrome c oxidase 1 (pcox1) mitochondrial gene region was performed on metacestode samples (hydatid cysts). The isolate was identified as sensu stricto genotype G1, which is most commonly found in Turkey.
Investigation of Some Markers of Inflammation in Sheep With Anaplasmosis
(Ataturk Universitesi, 2020) Bayram, G.; Mis, L.; Cinar, D.A.; Oguz, B.
Anaplasmosis is an arthropod-borne parasitic disease that infects blood cells and frequently seen in sheep. This study was planned to evaluate inflammation marker levels in sheep infected with anaplasmosis. In this research, the blood samples used were obtained from 20 healthy and 20 Anaplasmosis sheep brought to Van Metropolitan Municipality Slaughterhouse. Determination of control and patient groups, clinical symptoms of the disease, Giemsa stained blood smears and serological method (cELISA) were used. Serum samples of 91 sheep collected from Anaplasma spp. When examined serologically for the presence of antibodies of the species, 73.6% (67/91) were found to be seropositive in terms of Anaplasmosis. The blood of the sheep with high ELISA inhibition values and showing Anaplasma morulae form in the peripheral blood smears, were used for analysis. Granulocyte macrophage colony stimulating factor (GM-CSF), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) levels in 20 sheep whose positivity was detected were identified with the Elisa kit. IL-1β (ng/L), TNF-α (ng/L), GM-CSF (ng/L) levels from among the pro-inflammation parameters were found to increase. It is thought that researching the mechanisms related to inflammation developing against Anaplasma will contribute to studies for diagnosis and prevention of Anaplasma infections for future immunotherapy research. © 2020 Ataturk Universitesi. All rights reserved.
Molecular Detection and Phylogeny of Anaplasma Ovis in Goats in Van, Türkiye
(Springer Science and Business Media Deutschland GmbH, 2025) Oguz, B.; Deger, M.S.
One of the areas in Türkiye where animal husbandry is practiced extensively is the Eastern Anatolia Region. In the province of Van, which belongs to this region, depending on the sociocultural factors of the people, animal husbandry is carried out, especially in the form of small ruminant breeding. One of the most commonly reported intracellular tick-borne illnesses, Anaplasma ovis, infects ruminants worldwide and results in significant financial losses. The purpose of this study was to report the prevalence and phylogeny of this pathogen in goats that were obtained from Gevas, Van Province, Türkiye, via the msp4 gene. Blood samples were taken from the vena jugularis to the EDTA tubes from 205 randomly selected goats (apparently healthy) between April and June 2022. Using a pair of primers called MSP45/MSP43, the msp4 gene (major surface protein 4) of A. ovis was amplified from all the DNA samples via PCR. A total of 146 (71.2%) of the 205 samples amplified a fragment of 852 bp that was unique to the A. ovis msp4 gene. Sanger sequencing verified the presence of incomplete msp4 gene sequences, which were subsequently added to GenBank (PP625125–PP625128). None of the studied epidemiological characteristics (sex, age, infertility problem, history of abortion, or shelter conditions) were associated (P > 0.05) with A. ovis infection in the enrolled goats. Furthermore, there were variations in the prevalence of A. ovis in goats among the sampling sites. The analysis of the amplified partial msp4 sequence of A. ovis indicated that this gene is highly conserved, since all four sequences are phylogenetically closely related to each other and to the msp4 sequences amplified from other countries. The detection and phylogenetic analysis of A. ovis in this study have contributed to the existing knowledge of the parasite and provided valuable data on caprine anaplasmosis. Additionally, this research highlights the urgent need for further investigation and the implementation of targeted control measures in the region. © Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS) 2024.
Molecular Identification of Sarcocystis Species in Bovine Minced Meat Using Partial Cytochrome Oxidase Subunit 1 (Cox1) Gene in Van Province, Turkey
(Faculty of Veterinary Medicine, University of Forestry, 2022) Oguz, B.; Deger, M.S.
Sarcocystis species are obligate two-host protozoan parasites classified in the phylum Apicomplexa. Cattle are generally accepted to be the intermediate hosts for seven species, i.e., . S. cruzi, S. hirsuta, S. hominis, S. bovifelis, S. bovini, S. rommeli, and S. heydorni. Since it is not possible to differentiate between some species using amplification of the 18S ribosomal (rRNA) gen, the aim of this study was to reveal the molecular characterization of Sarcocystis species obtained from cattle minced meat amplifying partial cytochrome oxidase subunit 1 (Cox1) gene. Fifty DNA samples were used. Sequence analyzes of amplicons belonging to positive isolates were performed and their phylogenetic structures were investigated. While S. hominis was found in only one sample, it was molecularly confirmed that S. bovifelis was dominant species in other samples. We designed primer set in present study could not differentiate between S. bovini, S. rommeli and S. bovifelis species. Phylogenetic analyzes of isolates with GenBank records (OK041347-OK041353) were performed with similar isolates in the world. According to phylogenetic analysis, sequence of S. bovifelis (OK041347) was found closer to the isolates from cattle skeletal muscle in Argentina (KT900970 and KT900962). S. hominis (OK041352) isolate showed high genetic similarity to isolates from Netherlands and Italy (MK497840; MH021119). In conclusion, genetic characterization of S. bovifelis and S. hominis was performed for the first time in Van province of Turkey by partial cytochrome oxidase subunit 1 (Cox1) gene. © 2022 by the authors.
Molecular Investigation and Genotyping of Theileria Equi and Babesia Caballi in Horses in Mus Province, Turkey
(Hellenic Veterinary Medical Society, 2020) Oguz, B.; Özdal, N.; Deger, M.S.; Bicek, K.
Equine Piroplasmosis (EP) is a tick-borne disease caused by Theileria equi and Babesia caballi of the phylum Apicomplexa. In this study, 102 blood samples were randomly collected from the horses in Mus province of Turkey. PCR analysis, gene sequences, and phylogenetic analyses were carried out for detecting the presence and genotypic characteristics of species that cause piroplasmosis. Four (3.9%) of the 102 horses that were examined were found to be positive for T. equi, while B. caballi was not detected. Theileria equi isolates that were detected in the sequence analyses were found to be 100% identical to the isolates that were isolated from the horses in Turkey, the United States, and South Africa as well. In the phylogenetic analysis, all of the isolates were found to cluster with T. equi sequences in the genotype A. This study, in which we revealed intraspecies sequence heterogeneity of the parasite using the 18S rRNA gene region, provides important epidemiological data for equine piroplasmosis. However, we think that determining the characterization of genotypes that are common in different parts of our country is extremely important in terms of developing new diagnostic tools and vaccines. © 2020. All Rights Reserved.
Molecular Survey of Anaplasma Capra in Goats in Van Province, Eastern Türkiye
(Springer Science and Business Media Deutschland GmbH, 2024) Oguz, B.; Deger, M.S.; Al-Olayan, E.; El-Ashram, S.
Background: A newly discovered zoonotic infection carried by ixodid ticks, Anaplasma capra, affects a wide variety of hosts, including numerous mammals. A. capra most likely infects erythrocytes or endothelial cells in mammals. This study aimed to investigate the A. capra pathogen in goats in Türkiye’s Van province. Methods: A total of 200 goat blood samples were examined. Goat samples were subjected to partial amplification of the gltA gene fragment using a nested polymerase chain reaction. Results: A. capra DNA was detected in 0.5% of goat blood samples. Phylogenetic analysis of a partial gltA gene fragment showed that the Eastern Türkiye isolate, closely grouped with A. capra isolates reported from wild and domestic ruminants in France, Türkiye, and Kyrgyzstan, formed a distinct clade. Conclusions: This is the first report of A. capra in goats in Van province, Eastern Türkiye. © The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2023.
Total and Lipid-Bound Serum Sialic Acid and Hematological-Biochemical Gas Changes in Dogs With Dirofilariosis
(Hellenic veterinary Medical Soc, 2022) Kesici, H.; Deger, Y.; Oguz, B.; Ozdek, U.
The measurement of serum sialic acid concentration is of importance to indicate damage in cells or tissues. Dirofilaria immitis causes cardiovascular, pulmonary, hepatic and kidney lesions. Therefore, present study was planned to investigate the potential changes in the levels of serum total sialic acid (TSA), lipid-bound sialic acid (LSA) and hematological-biochemical parameters in the dogs naturally infected by Dirofilaria immitis.The patient group included the 7 dogs clinically and parasitologically (Modified Knott and PCR) diagnosed with Dirofilaria immitis while 7 healthy dogs were assigned as the control group. The biochemical parameters, blood gas parameters and hematological parameters were tested in the blood samples taken properly according to the guidelines from all the study animals using automated biochemistry analyzer, blood gas analyser and automated hematology analyzers, respectively. Serum TSA and LSA levels were measured spectrophotometrically using Sydow and Katapodis methods, respectively. Compared with the healthy group, the dogs diagnosed with dirofilariosis were found to have significantly increased levels of serum cardiactroponin, triglycerides, VLDL, LDL, BUN, urea and creatinine and enzymatic activities of CK-MB, ALT, AST, ALP and LDH, and statistically significantly decreased levels of cholesterol and HDL (p<0.05). A significantly decreased level of RBC and a significantly increased level of WBC was determined in the dogs with dirofilariosis (p<0.05). The differential leucocyte count test of the dogs with dirofilariosis indicated statistically significantly increased eosinophil count (p<0.05). In the dogs with dirofilariosis, the decreases in the levels of pCO(2), HCO3 and BE in venous blood were statistically significant (p<0.05). The levels of serum TSA and LSA of the dogs with dirofilariosis were found statistically significantly higher than the healthy group (p<0.05). As a conclusion, statistically significant differences were identified between the dogs with dirofilariosis and healthy dogs in terms of sialic acid levels and certain biochemical, venous blood gas and hematological parameters. On the other hand, the present study is the first to investigate the serum sialic acid levels in the dogs with dirofilariosis.