Browsing by Author "Oguz, Bekir"

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Anaplasma Phagocytophilum in Horses - Evaluation of Proinflammatory Biomarkers
(Univ Fed Rio Grande Do Sul, 2022) Mis, Leyla; Oguz, Bekir
Background: Anaplasma phagocytophilum is an obligate intracellular pathogen transmitted by the ticks that cause equine granulocytic anaplasmosis (EGA). This pathogen is infects predominantly blood cells, principally granulocytes and especially neutrophils. A. phagocytophilum causes an acute febrile disease in horses accompanying with lethargy, loss of appetite, lameness and hemorrhages. In horses, this disease should be considered in all acute symptoms accompanied by thrombocytopenia and leukopenia identified by hematological test performed. Tick-borne pathogens have become increasingly threatening for both animals and also public health since ticks mostly carry numerous well-documented and undocumented pathogens, and the geographical range of ticks has expanded in the recent years. This research has aimed to evaluate the impact of A. phagocytophilum infection on some oxidative/nitrosative stress parameters, antioxidant enzyme activities, proinflammatory biomarkers and trace element levels in horses. Materials, Methods & Results: The present study has been carried out using blood samples collected from 93 horses aged 1-year and older. The blood samples were centrifuged and sera were separated. Serum samples stored in the freezer (-20 degrees C) until the day of analysis. The DNA was extracted from blood and analysed by nested-PCR technique targeting 16S rRNA gene of A. phagocytophilum and then positive PCR products were sequenced. A. phagocytophilum was 6 horses (6.4%) showed positive nested-PCR results. An infected group comprised of 6 positive horses according to PCR analysis results also 6 healthy horses as control were selected. Serum SOD (Horse Superoxide Dismutase(Cu-Zn)) ELISA Kit, MPO (ELISA Assay Kit Horse Myeloperoxidase) and GPx (Horse glutathione peroxidase 1 ELISA Kit Assay), IL1 (Horse Interleukin 1 Beta ELISA Kit), IL6 (Horse Interleukin 6 ELISA Kit), TNF alpha (Horse Tumor Necrosis Factor Alpha ELISA Kit) and IL18 (Horse Interleukin 18 (IL18) ELISA Kit) levels were determined by ELISA reader. Serum TAS ,TOS Glucose-6-phosphate dehydrogenase (G6PD) levels were determined using colorimetric kit method. The determination of peroxynitrite was performed using spectrophotometry as described by Vanuffelen. The levels of Fe, Zn, Se, Cu, Mn, Ar, Cr, Co, Cd Ni and Pb elements were analyzed by ICP-OES. Total oxidant status (TOS) and peroxynitrite levels of the positive group infected with A. phagocytophilum were found to be higher compared with the control group (P < 0.05, respectively). Whereas, total antioxidant status (TAS), glutathione peroxidase (GPx) ,superoxide dismutase (SOD), G6PD and myeloperoxidase (MPO) levels were detected to be statistically significantly higher than the control group (P < 0.05, respectively). Cu, Mn, Se and Zn levels were also found to be lower in the infected group (P < 0.05, respectively). IL1, IL6, TNF alpha and IL18 from proinflammatory markers were elevated in the A. phagocytophilum infected group (P < 0.05, respectively). Discussion: The evaluation of the proinflammatory biomarkers with respect to host-parasite interactions has been suggested as a beneficial clinical tool for determination of the infection severity and diagnosis of the disease. As a conclusion, increased oxidative stress and high levels of some proinflammatory biomarkers assessed by the multibiomarker analysis carried out for the infection in the horses caused by Anaplasma phagocytophilum will provide a contribution to diagnosis, treatment and clarification of the pathogenesis.
Copro-Elisa Prevalence of Fasciola Hepatica in Cattle in Van, Turkey
(Univ Fed Rio Grande Do Sul, 2017) Bostanci, Aysegul; Oguz, Bekir
Background: Fasciolosis is an important food borne zoonotic disease caused by Fasciola trematode parasites. There are two types of Fasciola spp. namely F. hepatica and F. gigantica, widely distributed across the globe, affecting both human and animal hosts. In endemic regions, it is possible to base the diagnosis of fasciolosis on clinical signs and the season, however, it could be more useful to support these data with fecal examination and various hematologic and serological tests. The aim of this study was to determine the prevalence of fasciolosis in cattle in Van province by copro-ELISA technique. Materials, Methods & Results: Fecal samples from 140 cattle were technically collected and examined by sedimentation-zinc sulphate flotation technique. Modified McMaster sedimentation technique was applied to the egg positive samples to determine the EPG values. Fasciola hepatica coproantigens in samples were investigated by ELISA. The coprological and antigen ELISA prevalence of fasciolosis were determined as 5.07% and 30.7%, respectively, which shows the significant difference between these methods in examining the rate of infection. The highest prevalence of fasciolosis infection was observed in 1-2 age groups (41.9%), and this prevalence was followed by 3-5 (31.2%) and <= 6 age group (5%). The differences between age groups were found significant (P < 0.05). The prevalence in female and male cattle was found as 30.1% and 35.3% This difference was not found statistically significant (P > 0.05). The highest prevalence was observed in Brown Swiss with the ratio of 40% and this was followed by 31% in Crossbreed and 22.6% in Rubia Gallega. The differences among breeds were not statistically significant (P > 0.05). Discussion: Fasciola hepatica is the most common species of liver flukes, and its pathogenicity leads to significant impact on the economy of the livestock industry. The economic losses consist of costs of anthelmintics, drenches, labor, liver condemnation at meat inspection; and losses in production due to mortality, reduction in meat, milk and reduction in growth rate, fertility and decreased feed intake, conversion and lower resistance to other disease. To diagnose fasciolosis, eggs can only be detected in feces after the tenth or twelfth week of infection once the parasites have matured. It is reported that routine microscopic methods used before this stage do not provide sufficient information about the current infection status. Therefore, serological tests have been introduced for the early diagnosis of the disease. Among these tests, the ELISA test based on detecting antigens has become the most commonly used test. It is known that the probability of ELISA to cross-react with parasites that carry similar immunogenic features and the similarities between antibodies generated in previous infections and new infections pose a challenge to making the definitive diagnosis. Therefore, it is reported that to predict the parasitic potential of the host and the success of treatment beforehand, the presence of Fasciola spp. antigens can be investigated in serum instead of antibodies. In conclusion, this study has established prevalence of fasciolosis in cattle raised in Van province using the copro-ELISA technique for the first time. It has been concluded that copro-ELISA could serve as a useful technique for herd diagnosis of fasciolosis in cattle in addition to fecal examinations particularly with respect to fasciolosis.
Determination of the Levels of Serum Oxidative Indicator, Cytokine and Some Biochemical Parameters in Horses Naturally Infected With Theileria Equi
(Ankara Univ Press, 2020) Ozdek, Ugur; Oguz, Bekir; Komuroglu, Ufuk; Deger, Yeter
Equine theileriosis is considered as a serious problem because of harmful effects on the health and performance of equids. Despite the importance of this disease, there are few studies in related to clinical pathologic changes in equine theileriosis especially in horses. In this study, the oxidative stress biomarkers, cytokines, enzymes, lipid profile, electrolytes, minerals and some metabolites were evaluated in horses naturally infected with Theileria equi (n=22) and healthy (n=7). In infected horses, the serum concentrations of MDA, IFN-gamma, total protein, globulin, bilirubin (total, direct, indirect), triglyceride, glucose, iron, chloride, sodium and copper with enzyme activities of ALP, AST and GGT were found significantly higher, however concentrations of GSH, albumin, total cholesterol, HDL, TIBC, calcium, phosphorus, magnesium, potassium, zinc and manganese with enzyme activity of CAT were found lower when compared to the healthy horses (P<0.05). The changes in TNF-alpha, creatinine, urea and LDL concentrations were not statistically significant (P>0.05).The results indicate that there are significant changes in the oxidative indicator, cytokine, and biochemical parameters of horses in T. equi infection and that these changes may be useful in the evaluation of the diagnosis, prognosis and treatment of theileriosis. In addition, comprehensive studies are needed to better understand the role of cytokines in the pathogenesis of theileriosis.
Evaluation of Oxidative Stress, Thyroid Hormones, Trace Elements and Some Biochemical Markers in Goats Naturally Infected With Theileria Ovis
(Springer int Publ Ag, 2024) Caglar, Yunus Emre; Deger, Yeter; Oguz, Bekir
Background Theileriosis is a tick-borne disease caused by protozoon species in the Theileria genus of the Theileriidae family. The biochemical changes induced by infection are considered to be an important understanding of the pathophysiology of caprine theileriosis. In this study, it was aimed to determine oxidative stress, thyroid hormones, trace elements, and biochemical parameters in theileriosis infection. Materials and Methods A sample of 14 goat was used for this purpose, of which 7 were healthy and 7 were infected with Theileria ovis. Theileria infection was diagnosed from the polymerase chain reaction (PCR). Sera from blood samples was tested for total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), alanine aminotransferase (ALT), aspartate transaminase (AST), gamma glutamyl transferase (GGT), total protein, albumin, triglyceride, cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), urea, blood urea nitrogen (BUN), creatinine, triiodothyronine (T3), thyroxine (T4), copper (Cu), zinc (Zn), cobalt (Co), manganese (Mn), selenium (Se), iron (Fe). Result TOC, OSI, AST, ALT and GGT values were higher in the patient group than in the healthy group (P < 0.05). On the other hand, there were decreases in TAC, T3, T4, total protein, albumin, creatinine, Cu, Zn, Se, and Co values (P < 0.05). However, there was not found to be a statistical difference between the healthy and patient groups in terms of triglyceride, cholesterol, HDL, LDL, urea, BUN, Mn, and Fe values (P > 0.05). Conclusions It can be stated that oxidative stress is a complication of caprine theileriosis and it may be accompanied with hypothyroidism and deficits in trace minerals.
First Reports of Sarconema Eurycerca and Trinoton Anserinum in the Whooper Swan (Cygnus Cygnus) in Van, Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Oguz, Bekir; Kilinc, Ozlem Orunc; Deger, M. Serdar
Whooper swan Cygnus cygnus (Linnaeus, 1758) with wounded wing that found in the Ercis district of Van province was brought into Directorship of Wild Animal Protection of University of Yuzuncu Yil. Despite the surgical interventions, the whooper swan could not be rescued. Five lice in the feather and three nematodes in the heart were found at examination of the whooper swan. These lice specimens were identified as Trinoton anserinum (Fabricius, 1805). After the necropsy, nematodes were found in the heart of the whooper swan. Nematodes were identified as Sarconema eurycerca according to their morphological pecularities. Sarconema eurycerca have been reported for the first time in Whooper swan in Van, Turkey.
Genetic Analysis of Toxocara Spp. in Stray Cats and Dogs in Van Province, Eastern Turkey
(Walter de Gruyter Gmbh, 2018) Oguz, Bekir; Ozdal, Nalan; Deger, M. Serdar
Introduction: Toxocara canis and Toxocara cati are roundworms of dogs and cats. The purpose of this study was to investigate the infection caused by these ascarids in cats and dogs, using microscopic and molecular analysis methods. Material and Methods: Adult ascarids were gathered from the faeces of dogs and cats in Van province, in 2015-2016. Existing keys and PCR sequencing of the ITS-2 fragment were used to identify the morphological features of the parasite species. Results: It was observed that out of 20 adult ascarids, 17 and 3 were found to be Toxocara canis and Toxocara cati, respectively. The ITS-2 gene region was amplified by PCR to perform molecular analysis. Genotyping indicated that the dogs and cats were infected with T. canis and T. cati, respectively, and none had Toxascaris leonina. Conclusion: To the best of our knowledge, this is the first report on the molecular characteristics of adult ascaridoid nematodes from cats and dogs in Turkey. The molecular approaches established in this study enable molecular identification and genetic structure studies of the ascaridoids.
Genetic Characterization of Toxocara Vitulorum in Turkey by Mitochondrial Gene Markers (Cox1)
(Univ Fed Rio Grande Do Sul, 2018) Oguz, Bekir
Background: Toxocara vitulorum is a involved in the Ascaridoidea family and is a large roundworm with a semi translucent, soft body surface and pinkish color. Female worms measure 8-30cm in length, male worms 6-25cm. The major hosts of T. vitulorum are buffalo (Bubalus bubalis) and cattle (Bos species) in the humid tropics of Asia, Africa and South America. The diagnosis of T. vitulorum infections is usually made by observing characteristic eggs in routine fecal examination. Serological methods are also used to diagnose Toxocariasis. However, in recent years, PCR, a new generation molecular diagnostic method, has been used. The genetic structure of T. vitulorum is little known compared with data available from other parasites. The present sutudy was designed to determine the T. vitulorum isolates by the genetic characterization of the mitochondrial cytochrome c oxidase subunit I (cox1) gene. Materials, Methods & Results: Adult worms were collected from the feces of two calves (East Anatolian Red) during visits to the clinic at the Department of Internal Medicine of Van Yuzuncu Yil University, Faculty of Veterinary Medicine. Worms were washed thoroughly in 0.85 % saline to remove any debris and fixed into 70 % ethanol. After repeated and thoroughly washing the specimens, total genomic DNA of parasite extraction was performed be employing DNA extraction reagent kit (Thermo, GeneJET Genomic DNA Purification Kit) according to manufacturer's recommendations. After DNA amplification, a 446 bp fragment of cox1 of T. vitulorum were obtained in all three isolates. All generated sequences were registered in GenBank database with accession numbers including MG905159, MG911729 and MG911730. The cox1 of T. vitulorum examined differed from another two isolates extracted from Germany beef cattle (KY313642.1) and Sri Lanka buffalo calf (FJ664617.1) at NCBI database. The MEGA 7 software was employed to calculate intra-species distance and similarity. The intra-species distance rate and similarity among the isolates were 0.005 and 99.995%, respectively. The cox 1 sequence of T. vitulorum did not differ from an isolate from Germany, but differed more from isolate from Sri Lanka. The phylogenetic tree that was constructed using the Neighbor-Joining (NJ) method. Bootstrap support (Bp) for ML trees was calculating 1000 bootstrap replicates. This results indicate that both the different species of Toxocara are host-specific and each member of the genus Toxocara spp. has a different about the molecular sequences. We used the phylogenies from the Maximum Parsimony (MP) method to construct another phylogenetic tree based on the cox1 (mtDNA) gene. The results again display that the cattle-calves (East Anatolian Red) isolates from Turkey homology with that obtained from the Germany beef cattle (accession no. KY313642.1). Discussion: The genetic analysis of parasites is a crucial factor in terms of determining epidemiology and the control parasitic diseases of humans and animals. Toxocara vitulorum is the most common gastrointestinal helmints infecting ruminants particularly in tropical regions. Phylogenetic analysis revealed that T. vitulorum is 100% homology with related to sequence of T. vitulorum from Germany. The characterization of cox1 region can provides a foundation for accurate identification of some helminth species using PCR. Even though the small sample size, the obtained results might provide useful information for further phylogenetic studies on the family Ascaridae.
Investigating Erythrocyte Membrane Lipid and Protein Oxidation With Na+ Activity in Caprine Anaplasmosis
(Sivar-soc Italiana veterinari Animali Reddito, 2020) Ilkaya, Salim; Deger, Yeter; Oguz, Bekir; Ozdek, Ugur
Anaplasmosis is an infectious disease that is caused by the genus Anaplasma belonging to the family Anaplasmataceae in the order Rickettsiales and is seen in the mammals inhabiting tropical and subtropical climate zones. Anaplasma ovis and A. phagocytophilum are the most remarkable species causing anaplasmosis in goats. Anaplasmosis induces both cellular and humoral immunity. As animals develop a long-term immunity against the disease, it becomes difficult to treat the disease. Immunity develops mainly depending on premunition. Increased osmotic fragility of erythrocytes in animals infected with anaplasmosis has been found, and it has been reported that this may be developed by various immune-mediated mechanisms including oxidative damage. Moreover, it may also be associated with high cell membrane ATPase activity and erythrocyte morphological changes. The aim of this study was to evaluate the effect of anaplasmosis on erythrocyte membrane malondialdehyde (MDA), advanced oxidation protein products (AOPP), Sodium-potassium adenosine 5'-triphosphatase (Na+/K(+)ATPase), and hematological and biochemical parameters in goats. For this purpose, 45 male hair goats (35 infected and 10 healthy) that were brought to the slaughterhouse of Van Metropolitan Municipality were enrolled in the study. In infected goats, the hematological and biochemical concentrations of RBC, Hb, Hct, MCV, MCH, MCHC as well as serum total protein, albumin, total cholesterol and TIBC were found significantly lower, however concentrations of of WBC, globulin, total bilirubin, direct bilirubin, triglyceride, and iron as well as enzyme activities of AST, ALT, GGT were found higher when compared to the healthy goats (p<0.05). In addition, the MDA and AOPP levels were markedly increased in erythrocyte membrane from infected animals while the Na+/K(+)ATPase enzyme activity was significantly decreased compared to the the healthy goats (p<0.05). Therefore, it can be concluded that oxidative stress in erythrocyte membrane may play an important role in the pathogenesis of anemia in caprine anaplasmosis.
Molecular Detection of Nosema Spp. and Black Queen-Cell Virus in Honeybees in Van Province, Turkey
(Tubitak Scientific & Technological Research Council Turkey, 2017) Oguz, Bekir; Karapinar, Zeynep; Dincer, Ender; Deger, Mustafa Serdar
This study was planned to determine the prevalences of the Nosema spp. and the black queen-cell virus (BQCV) among honeybees (Apis mellifera) raised in the province of Van by PCR and to determine the molecular characteristics of the determined isolates. A total of 260 adult worker bees from 26 colonies at 5 apiary locations belonging to the province of Van in April and May 2015 were collected for this reason. Samples were examined microscopically. In the case of positivity, spore identification was done by multiplex PCR. Reverse transcription/PCR analysis (RT/PCR) was carried out for the BQCV analysis. At the end of the microscopic examination, Nosema spp. spores were detected in 8 out of 26 colonies (32.5%). The result of multiplex-PCR revealed Nosema ceranae positivity in all of the samples, but no Nosema apis was determined. As a result of the RT/PCR tests of the samples BQCV was detected in 23 (88.5%) of the total 26 colonies. This study is the first to investigate Nosema spp. and BQCV with the PCR technique in bees raised in the province of Van.
Molecular Identification Using 18s Ribosomal Rna of Sarcocystis Spp. in Bovine Minced Meat in Van Province, Turkey
(Ankara Univ Press, 2021) Oguz, Bekir; Deger, M. Serdar; Kosal, Sena
This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from Ipekyolu, Tusba, Edremit, Ercis, and Gevas districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study.
Phylogenetic Analysis of Black Queen Cell Virus and Deformed Wing Virus in Honeybee Colonies Infected by Mites in Van, Eastern Turkey
(Polish Soc veterinary Sciences Editorial office, 2018) Karapinar, Zeynep; Oguz, Bekir; Dincer, Ender; Ozturk, Cihat
This study aimed to determine the presence and prevalence of viral and parasitic infections causing high rates of colony loss in honey bee colonies in Van province, eastern Turkey. Twenty-six different apiaries were collected from five counties in Van province. These samples were tested by Reverse-Transcriptase PCR (RT-PCR) for acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), black queen cell virus (BQCV) and deformed wing virus (DWV). Selected positives were sequenced, phylogenetically analyzed and investigated in terms of Varroa. DWV and BQCV were identified in 69.23% (18/26) and 88.46% (23/26) of the bees respectively whereas ABPV and CBPV were not detected in the sampled apiaries. Results of the phylogenetic analysis of DWV and BQCV sequences showed 94-100% similarity to DWV and BQCV isolates obtained from Genbank. Prevalence of varroasis was 89% (23/26) in Van. The obtained samples were identified as Varroa destructor by morphological investigation. The study showed that viral and parasitic agents commonly infect honeybees in Van province, with high prevalence rates for BQCV and DWV. There was also a high degree of conservation of DWV and BQCV sequences distinct from DWV and BQCV isolates from other geographical regions. These findings, including current prevalence and phylogenetic analysis data for DWV, BQCV and varroazis in honeybees, are useful for future studies.
Preliminary Studies on the Prevalence and Genotyping of Echinococcus Granulosus Infection in Stray Dogs in Van Province, Turkey
(Walter de Gruyter Gmbh, 2018) Oguz, Bekir; Ozdal, Nalan; Kilinc, Ozlem Orunc; Deger, M. Serdar
Introduction: Echinococcus granulosus is a zoonotic helminth of the Taeniidae family living in the small intestines of dogs. The hydatid cyst, which is the larval form of this parasite, is observed in sheep, goat, cattle, and many other organisms including humans. It causes a disease called cystic echinococcosis. Identification of strains of E. granulosus in dogs is critical in parasite control and eradication where possible. This study aims to determine the genotype of E. granulosus eggs and prevalence of this parasite in the faeces of dogs in the Van Province using the copro-PCR method. Material and Methods: This study was conducted between 2015 and 2016 on the faeces obtained from 100 stray dogs from different parts of the Van Province. The coprological examination was conducted using the formalin-ether concentration method. Results: Taeniidae eggs were found in 10 (10%) out of 100 faecal samples. E. granulosus was detected in 4 out of 10 of these (40%) infected samples. Sequence analysis of positive amplicons obtained from PCR showed that there were sheep strains (G1). Conclusion: Dogs in Van area are primarily infected with the livestock genotype of E. granulosus, which is thought to be a potential zoonotic threat to humans.
Relationship Between Cardiac Injury, Selected Biochemical Parameters, Dic, and Hemogram Levels in Cattle With Theileriosis
(Polish Soc veterinary Sciences Editorial office, 2018) Kilinc, Ozlem Orunc; Ozdal, Nalan; Bicek, Kamile; Deger, M. Serdar; Yuksek, Nazmi; Yilmaz, Ali Bilgin; Oguz, Bekir
This study was undertaken to investigate the effects of theileriosis on the cardiovascular system in cattle. Blood samples were collected from 50 cattle naturally infected with Theileria spp. (before and after treatment) as well as from 30 non-infected cattle. Hematological parameters and the serum concentrations of total cardiac troponin I (cTnI), aspartate aminotransferase (AST), creatine kinase (CK), and creatine kinase MB (CK-MB) were measured. Also coagulation parameters, plasma levels of activated partial thrombo plastin time (APTT), prothrombin time (PT) and D-dimer were measured. The presence of anemia and the levels of cTnI, CK-MB, CK and AST indicated statistically significant differences between infected and healthy cattle, but no statistically significant differences between the two groups were found in DD levels at the end of the study. Reduction in anemia and reduced levels of cTnI, CK-MB, CK, AST, APTT and PT were also found in post-treatment analyses. The results indicate that anemia, coagulation disorders and hypoxia in theileriosis in cattle can induce myocardial tissue damage.
Several Tick-Borne Pathogenic Viruses in Circulation in Anatolia, Turkey
(Mary Ann Liebert, inc, 2022) Dincer, Ender; Timurkan, Mehmet Ozkan; Oguz, Bekir; Sahindokuyucu, Ismail; Sahan, Adem; Ekinci, Mustafa; Ergunay, Koray
Introduction: We screened host-collected ticks for tick-borne viruses, including those recently documented as human pathogens.Methods: During 2020-2021, ticks removed form cattle, sheep, dogs, and cats in 11 provinces in 5 geographically distinct regions of Anatolia were identified, pooled, and screened using pan-nairovirus, pan-flavivirus and individual assays for Jingmen tick virus (JMTV), and Tacheng tick virus 1 and 2 (TcTV-1 and TcTV-2).Results: A total of 901 tick specimens, comprising 6 species were included. Rhipicephalus sanguineus complex was the most abundant species (44.1%), followed by Rhipicephalus bursa (38.3%), Haemaphysalis parva (7.2%), and others. The specimens were screened in 158 pools with 12 pools (7.6%) being positive. Crimean-Congo hemorrhagic fever virus (CCHFV) lineage Europe 2 (genotype VI) sequences were detected in R. bursa in five (3.2%) of the pools, with similar prevalences in central and Mediterranean Anatolian provinces. JMTV was identified in four R. bursa and one Rhipicephalus turanicus pools, collected from Mediterranean and southeastern Anatolia, with a CCHFV and JMTV coinfected R. bursa pool. The JMTV segment 1 sequences formed a separate cluster with those from Turkey and the Balkan peninsula in the maximum likelihood analysis. TcTV-2 was detected in two Dermacentor marginatus specimens (1.3%) collected in central Anatolia, with nucleocapsid sequences forming a phylogenetically segregated group among viruses from humans and ticks from China and Kazakhstan.Discussion: CCHFV Europe 2 was initially documented in ticks from central Anatolian locations, where related orthonairoviruses had been previously recorded. Ongoing activity and a wider distribution of JMTV and TcTV-2 were observed. These viruses should be screened as potential etiological agents in human infections associated with tick bites.