Browsing by Author "Oguz, Fatma Ertas"

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The Investigation of Giardiasis (Foodborne and Waterborne Diseases) in Buffaloes in Van Region, Turkiye: First Molecular Report of Giardia Duodenalis Assemblage B From Buffaloes
(Mdpi, 2023) Kilinc, Ozlem Orunc; Ayan, Adnan; Celik, Burcak Aslan; Celik, Ozgur Yasar; Yuksek, Nazmi; Akyildiz, Gurkan; Oguz, Fatma Ertas
Giardia duodenalis (G. duodenalis) is an important zoonotic protozoan agent that causes foodborne and waterborne diarrhea in humans and other mammals. Molecular-based tests are critical in diagnosing giardiasis in humans and animals, identifying species, understanding the zoonotic potential and transmission routes, and evaluating taxonomy. Therefore, this study aimed to investigate the molecular characterization of G. duodenalis in buffaloes in the Van region in Turkiye. Buffaloes are a species that has been poorly studied in this regard. For this purpose, 100 fecal samples were collected from buffaloes in the Van region. The DNA extraction was performed using the GeneMATRIX STOOL DNA Purification Kit from stool samples. The nested PCR test was performed with the appropriate primers from the obtained DNA samples. The obtained bands suitable for sequencing were sent for sequence analysis, and the sequence results were aligned bidirectionally and compared with the database of GenBank by BLAST. As a result of the study, an 11% positivity rate for G. duodenalis was found in buffaloes, and assemblage E and assemblage B were isolated. To our knowledge, assemblage B in buffaloes was reported for the first time in this study. As a result, it was concluded that buffaloes are an important reservoir for waterborne and foodborne giardiasis.
Investigation of Interleukins and Oxidative Stress Parameters in Cows Naturally Infected With Bovine Viral Diarrhea Virus
(Univ Agriculture, Fac veterinary Science, 2024) Oguz, Fatma Ertas; Babaoglu, Ali Riza; Turkan, Fikret; Oguz, Ercan; Demirel, Ahmet Fatih; Pacal, Nurettin
Bovine Viral Diarrhea Virus (BVDV) can evade the immune system by modulating cytokines production, therefore enabling the virus to establish persistent infections or exacerbate the severity of disease in infected cattle. The objective of this research was to investigate interleukins and oxidative stress parameters in cows naturally infected with BVDV. The study comprised of two groups: a naturally infected group of 15 cattle with clinical signs of BVDV infection or a history of abortion and confirmed positive for pestivirus by RT-PCR, and a control group of 15 cattle with no abortion anamnesis or clinical symptoms, confirmed negative by RT-PCR. Anti-inflammatory cytokines (IL-4, IL-20, IL-10) and pro-inflammatory ones (IL-1, IL-1(3, IL-6) values were measured in all samples using ELISA method. Additionally, the oxidative stress marker malonaldehyde (MDA) was measured in all samples using the HPLC method. MDA levels were significantly higher in the infected samples compared with the controls. While cytokine levels were elevated in the infected group, however, the differences were not statistically significant. This study found a positive relationship among anti-inflammatory and pro-inflammatory cytokines, as well as a negative relationship among IL-1(3 and IL-1. It was concluded that oxidative stress occurs in BVDV-infected cattle, and the interleukins measured appear to remain in equilibrium by inhibiting each other. Notably, IL-20 was measured for the first time in BVDVinfected cattle, making it an important finding. Altogether, it may be concluded on the basis of these results that cytokines are important in the evaluation of the disease process.
Seroprevalence of Bovine Leukemia Virus in Cattle and Buffaloes in the Border Provinces of the Eastern Anatolia Region, Türkiye: Insights Into the Eradication of Infection
(Urmia Univ, 2024) Babaoglu, Ali Riza; Oguz, Fatma Ertas; Yilmaz, Volkan; Coskun, Nuvit; Abounaaja, Fatima
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an oncogenic deltaretrovirus that has emerged as a potential zoonotic infection. The BLV naturally infects cattle and causes economic losses through a slow persistent infection with various clinical symtoms following preleukosis. The main objective of this study was to determine the seroprevalence of BLV antibodies in cattle and buffaloes in the border provinces of the Eastern Anatolia region, T & uuml;rkiye, using the agar gel immunodiffusion (AGID) assay and enzyme-linked immunosorbent assay (ELISA). For this purpose, a total of 1,033 serum samples were collected from 982 cattle and 51 buffaloes from the provinces of A & gbreve;r & imath; (n = 178), I & gbreve;d & imath;r (n = 252), Kars (n = 317), Van (n = 221), and Hakkari (n = 65) during 2021- 2022. In AGID and ELISA tests, seropositivity for BLV-specific antibodies was not detected in cattle and buffaloes from the mentioned provinces. This study revealed that BLV was not circulating in cattle and buffaloes in the easternmost border provinces of T & uuml;rkiye during the sampling period and contributed to determine the status of BLV in the mentioned region. Due to the presence of virus in other regions of T & uuml;rkiye and neighboring countries, Iran and Iraq, it is recommended to control animal movements, continue efforts to combat the transmission of the virus, and maintain control measures. (c) 2024 Urmia University. All rights reserved.