Browsing by Author "Okyay, Ayse Guler"

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Biomechanical Effects of Menopause in Ovariectomized Rats' Femurs
(informa Healthcare, 2014) Okyay, Ayse Guler; Kavak, Servet; Turktas, Ugur; Alkis, Ismet; Guner, Savas; Aksakal, Bunyamin
Objective: To demonstrate the biomechanical changes in rat bones occurred by surgical menopause and to search for correlation between biomechanical test results and bone mineral density (BMD) measurements. Methods: Thirty female Wistar-albino rats aged 6 months and weighed 250-300 g were randomized into ovariectomy, sham and control groups. After 6 months, rats were sacrificed and right femurs were extracted. BMD measurements and three point bending biomechanical tests were made in femurs. Results: The difference among the mean BMD measurements of three groups were found to be statistically significant (Pr>F value <0.0001). Mean elastic module, maximum elongation and femoral diameters were also found to be statistically significantly different (Pr>F value <0.05). Ovariectomised and sham-operated groups were significantly different in terms of elastic modulus. Ultimate force did not differ among the groups significantly. The difference between ultimate strain and maximum elongation of the ovariectomy and sham operated group was statistically significant. Conclusions: Bone quality was deteriorated and fragility was increased in rats 6 months after surgical menopause. Sole BMD measurement might not be reliable in evaluation of osteoporosis and fracture risk. Biomechanical quality of bone is more important than its mineral content in determining fragility.
Biomechanical Effects of Menopause in Ovariectomized Rats' Femurs (Vol 30, Pg 62, 2014)
(informa Healthcare, 2014) Okyay, Ayse Guler; Kavak, Servet; Turktas, Ugur; Alkis, Ismet; Guner, Savas; Aksakal, Bunyamin
The Effect of Erythropoietin in Preventing Ischemia-Reperfusion Injury in Ovarian Tissue Transplantation
(Sage Publications inc, 2018) Kolusari, Ali; Okyay, Ayse Guler; Kockaya, Evrim Arzu
Condensation Erythropoietin improved the survival of follicles in ovarian grafts most likely by reducing ischemic injury, by improving neoangiogenesis, and by its antioxidant effects. Objective: Ovarian tissue cryopreservation and transplantation are the only options accepted for prepubertal girls and women requiring immediate chemotherapy. Ischemia-reperfusion injury is the main obstacle for ovarian tissue transplantation. In the present study, we aimed to evaluate the effects of recombinant human erythropoietin (EPO) on tissue viability in autotransplanted rat ovaries. Study Design: Seventeen female rats were randomized into 3 groups as sham control group (n = 5), EPO-treated group (n = 6), and EPO-untreated group (n = 6). Both ovaries were excised and transplanted into a subcutaneous pouch formed at the anterior abdominal wall in the EPO-treated and untreated groups. In the EPO group, 5000 U/kg EPO was applied as local injection to the site that ovarian tissue was placed and the dose was repeated with the same route at the end of the fourth week. After 2 months, ovaries were removed and blood samples were obtained. Levels of estradiol (E-2), vascular endothelial growth factor (VEGF), VEGF-C, and lipid hydroperoxidase (LPO) and the activity of glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT) were measured both in blood and tissue samples. Histopathological and morphometric analyses were also performed on tissue samples. Results: Considering serum levels, mean CAT was significantly higher (P = .003) and mean SOD (P = .033), LPO (P = .050), VEGF (P = .001), and VEGF-C (P = .024) were significantly lower in the EPO-treated group than in the untreated group. Mean serum GPX levels were similar. Significantly higher levels of E-2 were determined in the EPO group than in the untreated group. Highest serum E-2 levels were found in the sham group (P = .001). Tissue levels of GPX (1.23) and CAT (53.17) were significantly higher in the EPO group (P = .002 and P = .001, respectively). However, tissue levels of SOD and LPO, VEGF, and VEGF-C levels were significantly lower in the EPO group than those in the untreated group (P = .033, P = .050, P = .002, and P = .003, respectively). In tissue examination, the highest values of x, y axis and epithelial height were in the sham group. Mean value of the EPO group was found statistically significantly higher than that of the untreated group (P .05). In terms of antral follicle count, ordering was found as sham > EPO-treated > EPO-untreated group. Follicle counts in the EPO group were significantly higher than those in the untreated group (P 0.05). Conclusion: Erythropoietin improved the survival of follicles in ovarian grafts most likely by reducing ischemic injury, by improving neoangiogenesis, and by its antioxidant effects.
Spontaneous Pneumothorax and Pneumomediastinum in Pregnancy
(Galenos Yayincilik, 2014) Karadas, Sevdegul; Okyay, Ayse Guler; Odabasi, Dolunay; Selvi, Fatih
Spontaneous acute pneumothorax may occur as a result of spontanous rupture of subpleural blebs or bullae and it is extremely rare during pregnancy. Spontaneous acute pneumothorax and pneumomediastinum were diagnosed in a young pregnant woman admitted with the complaints of swelling and pain on her neck and upper thorax following 2 days' dyspnea. Nasal oxygen, analgesics and antiemetics were used as needed during ten days' hospitalisation. Physical findings, chest X-ray and oxygen saturation improved at the end of this period. Since supportive treatment was sufficient, invasive treatments such as surgery or thorax tube were not required. Although rare, pneumothoax should be remembered in any pregnant woman with dyspnea and chest-pain and must be confirmed radiographically to distinguish it from other diseases and conditions. In this report, a case of spontaneous acute pneumothorax and pneumomediastinum in a 10 week primigravida is presented.