Browsing by Author "Oner, Ahmet Cihat"

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Assessment of the Histopathological Changes Occurring in the Testis of the Mice Suffering From Experimental Diabetes Induced Using Alloxan
(Medwell online, 2009) Uslu, Baris Atalay; Ilhan, Fatma; Gulyuzf, Fetih; Karaca, Mehmet; Oner, Ahmet Cihat
In this study, we aimed to examine the effect of volatile oil extracts of Helichrysum plicatum DC (HP) and Tanacetum balsamita L. (TB) on the histopathology of the testicles of the mice that had alloxan-induced type 1 diabetes or with imitated physiopathology of type 2 diabetes. The volatile oil extracts of HP and TB; were injected intra. peritoneally in diabetic mice at 12.5, 25 and 50 mg kg(-1) doses. Control and reference groups received 0.2 mL serum physiologic intra peritoneally and oral glibenclamide at 0.3 mL kg(-1) dose, respectively. Blood samples were withdrawn from tail veins of the mice 1, 2 and 24 It after the treatment and the blood glucose levels were measured. We investigated testicular histopathology of the mice in control and diabetic groups. The examination of the testicles of diabetic mice revealed reduction in diameters of the seminiferous tubules and thickening in the wall of the seminiferous tubules in addition to degenerative changes and decline in the number of spermatogenic cells. There were multinucleated giant cells in the lumens of some seminiferous tubules. No significant shown was detected in the severity of lesions between control group of diabetic mice receiving serum physiologic and the diabetic mice treated with the extracts of H? and TB. TUNEL-positive cells were higher diabetic mice than in control mice. We monitored no marked encouraging effect of HP and TB volatile oil extracts on the testicular histopathology. The reason for this failure might be due to short term use of these extracts. Long term applications of volatile oil extracts of HP and TB at various doses remained to be done to elucidate the potential anti-diabetic effect of these extracts.
Drug Interaction Between Ivermectin and Etomidate: Investigation on the Effect of Ivermectin on Etomidate Anaesthesia
(Soc Stiinte Farmaceutice Romania, 2020) Yucel, Ufuk Mercan; Oner, Ahmet Cihat; Turel, Idris; Yilmaz, Orhan
The present study aims to determine the effect of ivermectin on the duration of anaesthesia performed with etomidate and to find out if there are any interactions between these two drugs at the central nervous system level. For this purpose, 25 Sprague-Dawley female rats were divided into 5 groups with 5 rats in each group. Group I received only subcutaneous saline at a dosage of 0.1 ml/100 g bw. Group II received the intraperitoneal injection of propylene glycol at a dosage of 1 mL/100 g bw. Group III received the subcutaneously 0.2 mg/kg bw ivermectin. Two hours after these treatments, neuropharmacological tests (rotarod and open field) were performed. Also, Group IV received 20 mg/kg bw etomidate intraperitoneally. Group V received subcutaneously 0.2 mg/kg bw ivermectin and 2 hours after the ivermectin administration, 20 mg/kg bw etomidate was given intraperitoneally. In these two groups, anaesthesia was monitored and the sleep duration was calculated based on rectal reflexes. Once the animals were fully awake, neuropharmacological tests were performed. Ivermectin that was administered two hours before the etomidate anaesthesia prolonged etomidate sleeping duration strongly. Also, ivermectin increased motion and decreased motor coordination, in association with etomidate it potentiates CNS depression (with prolonged sleep duration and decreased motor coordination).
The Effect of Hcg Administration on Reproductive Performance in Undernourished Lactating Hair Goats Synchronized During Non-Breeding Season
(veterinarni A Farmaceuticka Univerzita Brno, 2024) Cetin, Nebi; Kosal, Volkan; Kuru, Mushap; Oner, Ahmet Cihat; Eski, Funda
This study aimed to assess the effects of short and long synchronization protocols, combined with post-synchronization human chorionic gonadotropin (hCG) administration, on the reproductive performance of lactating hair goats during the non-breeding season, considering their inadequate pasture conditions. A total of 60 goats were randomly divided into four groups used for 5 days in G1 and G2 and 12 days in G3 and G4. All received a 500 IU pregnant mare's serum gonadotropin (PMSG) injection 48 h before sponge removal and were exposed to bucks 12 h later. Groups G2 and G4 received a 500 IU hCG injection on the eighth day after mating. Groups G1 and G3 did not receive any application after mating. Blood samples were collected on the 8th, 15th, and 22nd days for post-mating progesterone analysis, and pregnancy examinations were performed on the 35th day. The study showed a 90% total oestrus rate (54/60). However, there were no significant differences in conception, pregnancy, and kidding rates among the groups. Serum progesterone concentrations significantly increased on the 15th day in G2 and G4, where hCG was administered. In summary, hCG raised progesterone levels but did not significantly affect the reproductive performance of undernourished, lactating goats in a nonbreeding season, suggesting that environmental factors and animal nutrition play a crucial role in synchronization outcomes.
Effect of Imidocarb on Dna Damage in Sheep With Babesiosis
(Kafkas Univ, veteriner Fakultesi dergisi, 2022) Oner, Ahmet Cihat; Ayan, Adnan; Orunc Kilinc, Ozlem; Usta, Ayse; Ertas, Fatma
In this study, it was aimed to determine the DNA damage using the comet assay, which specifically shows DNA damage in naturally Babesia spp.-infected sheep and to evaluate the damage before and after imidocarb application. Blood samples obtained from 10 infected sheep with positive clinical signs and symptoms of babesiosis and whose diagnosis was confirmed by Giemsa staining and PCR methods, and blood samples from 10 healthy sheep were used as study material. DNA damage was examined by the comet assay from the blood samples of the infected patient group and the control group obtained during the disease and after the treatment, and the results were compared with statistical methods. When DNA damage was examined in sick animals diagnosed with babesiosis, the tail length and the tail moment values were found to be statistically significantly higher than the control group (P<0.0001). According to the results obtained after imidocarb application, it was determined that DNA damage and tail moment decreased statistically with imidocarb, and the difference was statistically significant, and the values were higher than the control group (P<0.0001). As a result, Babesia infection can cause DNA damage, has been confirmed by the determination of direct DNA damage using the comet assay, and imidocarb given for treatment was successful and reduced the damage.
The Effect of Vitamin C and Vitamin E on Dna Damage, Oxidative Status, and Some Biochemical Parameters in Rats With Experimental Fluorosis
(int Soc Fluoride Research, 2020) Oner, Ahmet Cihat; Dede, Semiha; Yur, Fatmagul; Oner, Aysegul
Excess fluoride intake may lead to metabolic, functional, and structural damage in soft tissue and organs as well as in teeth and bones. The aim of the present study was to investigate the protective and therapeutic properties of vitamins C (vit C) and E (vit E) on the oxidative status and balance, DNA damage, and some biochemical parameters in rats with experimentally-induced chronic fluorosis. Seventy-two Wistar-Albino rats were divided into 9 groups, each containing 8 rats. Control groups were designed as real control and vehicle control groups. Vit C (100 mg/kg), vit E (300 mg/kg) and vit C + vit E (100 mg/kg + 300 mg/kg, respectively) in water that contained 150 ppm NaF were administered daily to three protection groups over 16 weeks. Three treatment groups received ad libitum water with 150 ppm NaF for 16 weeks, followed by the administration of normal drinking water plus vit C (100 mg/kg), vit E (300 mg/kg), and vit C + vit E (100 mg/kg + 300 mg/kg, respectively) on alternate days for 4 weeks. Serum biochemistry, total antioxidant status (TAS), total oxidant status (TOS), and analyses were conducted on sera obtained at the end of the study. To determine DNA damage, the comet assay and 8-hydroxy-2'-deoxyguanine (8-OHdG) analysis were used. Although the therapeutic and protective vitamin administration of vitamins C and E to the rats with experimentally-induced chronic fluorosis had only a limited effect on the oxidative status, the biochemical results for renal and hepatic function showed that the vitamins had positive effects and the comet assay and 8-OHdG analysis showed that DNA damage was reduced. In conclusion, significant positive results were obtained with protective and therapeutic treatment with vitamins E and C in rats with experimentally-induced chronic fluorosis. It is suggested that further studies are conducted to clarify the molecular mechanisms involved in producing these positive outcomes.
Hepatoprotective and Anti-Inflammatory Activities of Plantago Major L
(Wolters Kluwer Medknow Publications, 2009) Turel, Idris; Ozbek, Hanefi; Erten, Remzi; Oner, Ahmet Cihat; Cengiz, Nureddin; Yilmaz, Orhan
Objective: The aim of this study was to investigate anti-inflammatory and hepatoprotective activities of Plantago major L. (PM). Materials and Methods: Anti-inflammatory activity: Control and reference groups were administered isotonic saline solution (ISS) and indomethacin, respectively. Plantago major groups were injected PM in doses of 5 mg/kg (PM-I), 10 mg/kg (PM-II), 20 mg/kg (PM-III) and 25 mg/kg (PM-IV). Before and three hours after the injections, the volume of right hind-paw of rats was measured using a plethysmometer. Hepatoprotective Activity: The hepatotoxicity was induced by carbon tetrachloride (CCl4) administration. Control, CCl4 and reference groups received isotonic saline solution, CCl4 and silibinin, respectively. Plantago major groups received CCl4 (0.8 ml/kg) and PM in doses of 10, 20 and 25 mg/kg, respectively for seven days. Blood samples and liver were collected on the 8th day after the animals were killed. Results: Plantago major had an anti-inflammatory effect matching to that of control group at doses of 20 and 25 mg/kg. It was found that reduction in the inflammation was 90.01% with indomethacin, 3.10% with PM-I, 41.56% with PM-II, 45.87% with PM-III and 49.76% with PM-IV. Median effective dose (ED50) value of PM was found to be 7.507 mg/kg. Plantago major (25 mg/kg) significantly reduced the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels when compared to the CCl4 group. The histopathological findings showed a significant difference between the PM (25 mg/kg) and CCl4 groups. Conclusion: The results showed that PM had a considerable anti-inflammatory and hepatoprotective activities.
Quinazolinone-Based Benzenesulfonamides With Low Toxicity and High Affinity as Monoamine Oxidase-A Inhibitors: Synthesis, Biological Evaluation and Induced-Fit Docking Studies
(Academic Press inc Elsevier Science, 2022) Yamali, Cem; Gul, Halise Inci; Sakarya, Mehtap Tugrak; Saglik, Begum Nurpelin; Ece, Abdulilah; Demirel, Goksun; Oner, Ahmet Cihat
The research in selective monoamine oxidases (MAO-A and MAO-B) inhibitors has been increased due to their therapeutic value for neurodegenerative diseases. In this study, 4-((2-(aryl)-4-oxoquinazolin-3(4H)-yl)amino) benzenesulfonamides were synthesized and their MAOs inhibition potentials were investigated applying in vitro fluorometric technique. The most potent compounds 7 and 8 against MAO-A had IC50 values of 0.058 +/- 0.002 and 0.094 +/- 0.003 mu M, respectively, while the reference moclobemide had an IC50 value of 6.061 mu M. Compounds 7 (> 1724 times) and 8 (> 1063 times) more selective and reversible inhibitors of MAO-A rather than MAO-B. Toxicity studies of 7 (IC50 = 210.23 mu M) and 8 (IC50 = 259.27 mu M) showed that compounds can be considered as non-toxic towards SH-SY5Y cell line at their effective concentrations against MAO-A. In silico docking simulations successfully explained the observed activities and also highlighted structural water molecules to play a key role in the ligand-enzyme interactions. Calculated molecular descriptors are also obeying Lipinski's rule of five and brain/blood partition coefficients, a critical parameter in neurodegenerative diseases. These reversible inhibitors can have considerable advantages compared to irreversible inhibitors which may possess serious pharmacological side effects.