Browsing by Author "Orgi, Elif"

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Anatomical and Histological Investigation of the Pineal Gland in the Lake Van Fish (Alburnus Tarichi (Guldenstadt, 1814))
(Wiley, 2022) Orgi, Elif; Oguz, Ahmet Regaib
The pineal gland and melatonin secreted from the gland regulate the biological clock and adaptation to seasonal changes, glucose balance, nutrition and locomotor activities. In this study, the pineal gland of the Lake Van fish was examined anatomically and histologically. The melatonin level secreted from the pineal gland was determined in fish plasma sampled from both lakes and streams during reproduction migration. The pineal gland in the Lake Van fish, as in other teleost fish, is located in the head, under the translucent pineal window, which does not contain many pigment cells. The gland consists of pineal vesicle and pineal stalk parts on the dorsal sac in the Lake Van fish. It was determined that the pineal gland showed good vascularity. The presence of pinealocytes and different types of cells in the pineal organ was determined histologically. Pinealocytes were intensely localized in the lumen of the pineal vesicle. The plasma melatonin level increased in fish passing from lake to stream for reproductive migration.
Histopathological Determination of Changes in Tissues of Lake Van Fish (Alburnus Tarichi (Güldenstädt, 1814)) Exposed To Esfenvalerate
(Taylor & Francis Ltd, 2024) Oguz, Elif Kaval; Alkan, Zehra; Oguz, Ahmet Regaib; Azizoglu, Burcu Ergoz; Orgi, Elif
Esfenvalerate is a widely used pesticide of the pyrethroid insecticide group. It is a hydrophobic pesticide with toxic effects on many aquatic organisms. In this study, the damage caused by esfenvalerate 0.158 mu g/L administered sublethally to the gills, liver, and kidneys of Lake Van fish was determined histopathologically. Lesions such as necrosis, hyperplasia, epithelial lifting, hypertrophy, and haemorrhage were observed in the gills exposed to esfenvalerate. Gill goblet cells increased after 24 h and decreased on the other days of esfenvalerate exposure. Histopathological changes such as haemorrhage, hypertrophy, necrosis, and cloudy degeneration were observed in the liver. In the kidneys, lesions such as haemorrhage, necrosis, cell degeneration, Bowman's capsule enlargement, and glomerulus atrophy were detected. These lesions increased in tissues depending on the length of application of esfenvalerate. As a result of this study, it was determined that esfenvalerate caused damage to the gills, liver, and kidneys in Lake Van fish depending on time. These anomalies observed in the gills, liver, and kidneys can be used as a bioindicator of esfenvalerate contamination in Lake Van.
Immunohistochemical Examination of Hsp70, Pcna, 8-Ohdg, Inos and Apoptosis in the Gills of Lake Van Fish (Alburnus Tarichi (Güldenstädt, 1814)) After Esfenvalerate Exposure
(Taylor & Francis Ltd, 2025) Cekic, Zehra Alkan; Orgi, Elif; Oguz, Ahmet Regaib; Otun, Yagmur
Lake Van fish is an endemic carp species adapted to the soda waters of Lake Van. As a result of agricultural and livestock activities around the lake, many environmental pollutants are dumped into the lake as waste. Esfenvalerate is a pyrethroid pesticide used extensively in agricultural areas in the region. In this study, Lake Van fish were exposed to a sublethal dose of esfenvalerate (0.158 mu g/L) for 24, 48, 72, and 96 hours, and their gill tissues were examined immunohistochemically. Histopathological analysis determined that hyperplasia and necrosis occurred in the gill tissue as a result of exposure. Additionally, the heat shock protein 70 (HSP70), proliferating cell nuclear antigen (PCNA), 8-hydroxy-2' -deoxyguanosine (8-OHdG), and nitric oxide synthase (iNOS) positive cells and the number of apoptotic cells were determined by immunohistochemistry in gills of Lake Van fish exposed to esfenvalerate. The density of cells containing HSP70 and PCNA increased at 24 and 48 hours of exposure to esfenvalerate. 8-OHdG occurred in gill tissue as a result of esfenvalerate exposure, and the number of damaged cells increased over time. The number of apoptotic cells and containing the inducible isoform of iNOS increased in the gill tissue as a result of esfevalerate exposure. Overall, it was determined that esfenvalerate had a toxic effect on Lake Van fish gills.
Investigation of the Therapeutic Effect of Melatonin on Deltamethrin Applied Mouse Primary Hepatocyte Culture
(Taylor & Francis Ltd, 2025) Kaval Oguz, Elif; Oguz, Ahmet Regaib; Ozok, Necati; Alkan, Zehra; Ergoz Azizoglu, Burcu; Orgi, Elif; Yesilbas, Ayse
ObjectiveIn recent years, it has been known that the melatonin hormone, secreted from the pineal gland, possesses significant antioxidant activity. This study explores the therapeutic effect of melatonin on the deleterious effects of deltamethrin, a pyrethroid pesticide extensively used worldwide, including in T & uuml;rkiye, on mouse liver cells.MethodsHepatocytes from Balb/C mice were isolated using a two-stage perfusion method, resulting in over 85% live hepatocytes. The isolated cells were cultured with different doses of deltamethrin (1 and 10 mu M) and melatonin (100 mu M) for 24 and 48 hours. At the conclusion of the culture period, hepatocytes were extracted at the 24th and 48th hours, and Malondialdehyde (MDA), Total Antioxidant Capacity (TAC), Total Oxidation Status (TOS), and DNA damages (8-hydroxy-2'-deoxyguanosine (8-OHdG)) were examined.ResultsWhile an increase in MDA, TOS, and DNA damage was observed in the deltamethrin-administered groups of hepatocytes, a decrease in TAC level was noted. It was determined that the applied deltamethrin had no effect on cell viability throughout the application period.ConclusionFurthermore, it was observed that melatonin, when administered concurrently with deltamethrin, reduced the toxic effect of deltamethrin. This study suggests that melatonin has a protective effect against deltamethrin-induced damage in mouse hepatocyte cells.