Browsing by Author "Orunc Kilinc, Ozlem"

Now showing 1 - 5 of 5

Determination of Serum Cardiac Biomarkers and Plasma D-Dimer Levels in Anemic Sheep With Babesiosis
(Tubitak Scientific & Technological Research Council Turkey, 2015) Orunc Kilinc, Ozlem; Goz, Yasar; Yuksek, Nazmi; Basbugan, Yildiray; Yilmaz, Ali Bilgin; Atas, Ahmet Duran
In this study, the cardiac effects of anemia and venous thromboembolism (VTE), reportedly caused by the hemolyzed erythrocytes occurring in sheep with babesiosis, were investigated using cardiac markers and D-dimer (DD). The study included 34 sheep: 24 infected Akkaraman sheep (1-3 years old, diagnosed with babesiosis based on clinical and laboratory findings) and a control group of 10 noninfected healthy sheep of the same breed and age. Hematocrit (Hct) levels were measured in blood obtained from both groups. Cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and aspartate aminotransferase (AST) levels were measured in serum samples. In addition, the levels of DD were also measured in plasma samples. Sheep with babesiosis were subsequently divided into 3 subgroups according to their Hct levels, which ranged from 13.2% to 16.3% in the first group (n = 8), 20.1% to 25.6% in the second group (n = 8), and 27.4% to 30.3% in the third group (n = 8). Evaluations of the measurements of cTnI, CK-MB, and AST levels indicated statistically significant differences between infected and healthy sheep. Statistically significant differences were not found for DD levels between the 2 groups.
Effect of Imidocarb on Dna Damage in Sheep With Babesiosis
(Kafkas Univ, veteriner Fakultesi dergisi, 2022) Oner, Ahmet Cihat; Ayan, Adnan; Orunc Kilinc, Ozlem; Usta, Ayse; Ertas, Fatma
In this study, it was aimed to determine the DNA damage using the comet assay, which specifically shows DNA damage in naturally Babesia spp.-infected sheep and to evaluate the damage before and after imidocarb application. Blood samples obtained from 10 infected sheep with positive clinical signs and symptoms of babesiosis and whose diagnosis was confirmed by Giemsa staining and PCR methods, and blood samples from 10 healthy sheep were used as study material. DNA damage was examined by the comet assay from the blood samples of the infected patient group and the control group obtained during the disease and after the treatment, and the results were compared with statistical methods. When DNA damage was examined in sick animals diagnosed with babesiosis, the tail length and the tail moment values were found to be statistically significantly higher than the control group (P<0.0001). According to the results obtained after imidocarb application, it was determined that DNA damage and tail moment decreased statistically with imidocarb, and the difference was statistically significant, and the values were higher than the control group (P<0.0001). As a result, Babesia infection can cause DNA damage, has been confirmed by the determination of direct DNA damage using the comet assay, and imidocarb given for treatment was successful and reduced the damage.
First Report on Heavy Uncinaria (Dochmoides) Sp (Nematoda: Ancylostomatidae) Infection in Brown Bear (Ursus Arctos) Cub, in Van Province, Eastern Anatolian Region of Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Orunc Kilinc, Ozlem; Goz, Yasar; Yilmaz, Ali Bilgin; Aslan, Logman
On April 2014, 6 months old male Brown bear cub that found in the Saray district of Van province was brought into Directorship of Wild Animal Protection of University of Yuzuncu Yil. Despite all the intervation, bear cub, very poor condition in clinically, dead after two days. In postmortem patological and parasitological examination were detecded severe hemorrhagic enteritis and many hookworms with white colour and approximately 1 cm diameter in surface of the small intestine. In stereo microscopic examinations, it was concluded that this worms are Uncinaria spp. This case report represents the first time Uncinaria spp. have been reported in Brown bear in Turkey.
Molecular Diagnosis of Toxoplasma Gondii and Neospora Caninum in Brain Tissues of Some Wild Birds
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Muz, Mustafa Necati; Orunc Kilinc, Ozlem; Isler, Cafer Tayer; Altug, Enes; Karakavuk, Muhammet
There are limited molecular studies about Toxoplasma gondii and Neospora caninum which are economically important livestock protozoons in wild birds investigated by polymerase chain reaction (PCR) method. Molecular prevalance of both parasites in brain tissues of wild birds in Turkey is unknown. Prevalance of T. gondii was 7%, N. caninum was 14% and mix infection was found 4% in brain tissues of 101 wild birds under 20 species from two different regions of Turkey. The chi-square test has been applied to the acquired data. This is the first molecular biologic investigation for the aim of PCR diagnosis of T. gondii in brain tissues of Corvus corone, Melanitta fusca, Aquila heliaca, Aquila pomarina, Buteo rufinus, Accipiter nisus, Strix aluco and N. caninum in brain tissues of Larus genei, Corvus corone, Melanitta fusca, Anas clypeata, Perdix perdix, Aquila heliaca, Buteo rufinus in the world. This also is the first molecular diagnostic investigation of T. gondii and N. caninum in brain tissues of wild birds in Turkey.
Preliminary Investigation of the Prevalence and Genotype Distribution of Cryptosporidium Spp., and Giardia Duodenalis in Cats in Siirt, Turkey
(Sciendo, 2023) Aslan Celik, Burcak; Celik, Ozgur Yasar; Ayan, Adnan; Akyildiz, Gurkan; Orunc Kilinc, Ozlem; Oktay Ayan, Ozge; Ercan, Kerem
Cryptosporidium spp. and Giardia duodenalis are protozoan parasites found in humans and many animal species worldwide. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in cats and to evaluate the zoonotic potential of these agents. The animal material of the study consisted of a total of 40 cats brought to the Veterinary Faculty. Fresh fecal samples taken from the cats were placed in individual sample containers. All samples were examined under the microscope by Kinyoun Acid Fast staining for Cryptosporidium spp. and by the native-Lugol method for Giardia duodenalis. Nested PCR and sequence analyses were then performed. As a result of microscopic and nested PCR analyses for Cryptosporidium spp., no positivity was found in any sample. The prevalence of Giardia duodenalis was 2.5% in both microscopic examination and nested PCR analyses. When the DNA sequences of the beta-Giardin gene obtained in the study were compared with the database in NCBI Basic Local Alignment Search Tool, it was determined that one sample overlapped with Assemblage B samples. As a result of this study, the prevalence of Cryptosporidium spp. and Giardia duodenalis in cats was determined and the presence of Assemblage B was revealed. It is recommended that repetitive studies should be carried out as much as possible to determine the possible role of these parasites in the transmission of these parasites to humans.