Browsing by Author "Oto, G."

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Antinociceptive Activity of Aqueous Extract of Lepidium Sativum L. in Mice
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Özdemir, H.; Yaren, B.; Oto, G.
In the present study the aqueous extract of Lepidium sativum L. (family: Brassicaceae) was investigated for possible antinociceptive effect in Swiss - albino male mice. In this experiment three groups of male mice were used (n=6). Two models were used to study the effects of the extracts on nociception, acetic acid-induced writhing test and hot plate test in mice. Lepidium sativum L. extract was administered in the dose of 20 mg/kg orally 30 minutes prior to pain induction. The aqueous extract showed significant (p<0.05) analgesic activity evidenced by increase in the reaction time by hot plate method and significant (p<0.05) reduction in acetic acid - induced writhings in mice with a maximum effect of 27.00% reduction. These effects were compared with the control and standard drug, diclofenac sodium (50 mg/kg, p.o). The results indicate that aqueous extract of Lepidium sativum L. possesses a significant antinociceptive activity in central and peripheral pain models in mice and therefore, it can be used as supplemental therapy in acute or chronic pain conditions. © 2015, Yuzuncu Yil Universitesi Tip Fakultesi, Universitas Indonesia. All rights reserved.
Changes in Sperm Parameters Following Administration of Theophylline, a Competitive Antagonist of Adenosine in Rats Exposed To Bleomycin, a Chemotherapeutic Agent
(Ataturk Universitesi, 2019) Belhan, S.; Oto, G.; Arihan, O.; Koşal, V.
The aim of the present study is to determine the effects of theophylline (THP), a methylxanthine derivative, on the sperm parameters of rats exposed to bleomycin (BLM) as a chemotherapeutic agent. The study was performed on 32 male rats. The rats in Group 1 were administered only with sterile saline intratracheally. The rats in Group 2 were administered a single dose of 7.5 mg/kg of BLM intratracheally. The rats in Group 3 received a single dose of 7.5 mg/kg of BLM intratracheally. In addition, THP was administered to rats in Group 3 intraperitoneally at a dose of 75 mg/kg/ day for 14 days. The rats in group 4 were administered an intraperitoneal injection of THP at a dose of 75 mg/kg/day for 14 days. Motility, sperm density, abnormal sperm and dead sperm rates were examined. Significant deteriorations were observed in sperm parameters of rats that were administered BLM. In sperm parameters of rats that were administered THP, it was determined that it only increased motility, but did not significantly change sperm density, abnormal sperm and dead sperm count. In this study, THP only increased motility. There was no positive effect on sperm parameters except motility. © 2019 Ataturk Universitesi. All rights reserved.
The Changes in the Serum, Liver, Kidney Protein and Genomic Dna Profiles in Rats Treated With 7,12-Dimethylbenz(a) Anthracene and Plantago Major L
(2014) Berber, I.; Ekin, S.; Levent, A.; Oto, G.
In the study, the changes in the serum, liver, kidney protein and genomic DNA profiles in Wistar albino rats administrated to 7,12-dimethylbenz(a)anthracene (DMBA) and Plantagomajor L. were investigated. SDS-PAGE profiling of the serum proteins showed that the levels of two proteins molecular weighing 140.8 kDa and 46.8 kDa were significantly lower on the 0th day of treatment than on the 60th day of application in the groups. Furthermore, two proteins (34.5 and 22.3 kDa) were solely present in DMBA-treated liver cell lysates. On the contrary, the kidney protein profiles did not show significant banding variations in groups. Genomic DNA analysis also confirmed that DNA extracted from liver and kidney cells did not fragment in any of the groups after the 60th study day. The proteins 34.5 and 22.3 kDa in liver cell lysates at the 60th day in DMBA-treated group could be extra-prognostic indicators for severe DMBA toxicity.
Chronic Immobilization Stress in Rats: Fluoxetine and Amisulpride Protects Against Chronic Immobilization Stress-Induced Biochemical Alterations
(Wiley-blackwell, 2016) Oto, G.; Ekin, S.; Karakus, Y.; Oksuz, E.; Ustun, R.; Ozdemir, H.
Effect of Alloxan on Urea, Creatine and Bilirubin Levels in Serum of Rats
(Blackwell Publishing, 2007) Cebi, A.; Yasar, S.; Oto, G.; Demir, H.
The Effect of Chronic Exposure To Fluorine and 7,12-Dimethylbenz(a)anthracene on Anxiety, Locomotor Activity, Spatial Learning, and Memory Consolidation in Rats
(int Soc Fluoride Research, 2020) Uyar, H.; Oto, G.
In this study, the effect of fluorine, which is known to cause chronic toxication, and 7,12-dimethylbenz(a)nthracene (DMBA) on anxiety, locomotor activity, spatial learning, and memory consolidation in rats were investigated. Ninety adult Wistar albino male rats, weighing 150-200 g, were divided into 9 groups of 10 animals and treated for 90 days with fluoride (F), the ion of fluorine, in drinking water in the form of sodium fluoride (NaF) and weekly oral DMBA (10 mg/kg bw/po/weekly) in a sesame oil vehicle, in the following manner: group 1: 0 ppm F, 0 mg/kg bw DMBA (control); group 2: sesame oil weekly (vehicle control); group 3: 1 ppm F; group 4: 15 ppm F; group 5: 30 ppm F; group 6: DMBA; group 7: 1 ppm F and DMBA; group 8: 15 ppm F and DMBA; and group 9: 30 ppm F and DMBA. Tests for anxiety (elevated plus maze), locomotor activity (rotarod), and spatial learning and learning consolidation (Morris water maze) were administered to rats at the beginning and at the 30th, 60th, and 90th days of the study. The results showed that anxiety was increased by F at a low dose and DMBA, and decreased by high dose F. Locomotor activity was increased by F and decreased by F+DMBA. DMBA could either increase or decrease motor activity depending on the time of assessment. Spatial learning and memory consolidation were decreased by both F and DMBA. After considering these results, we plan to conduct further studies to clarify the mechanisms underlying these neurotoxic effects of fluorine and DMBA.
Effect of Estrogen/Drospirenon Combinations and Tibolone on Biochemical Parameters and the Endometrium in Ovariectomized Rats
(Wiley-blackwell, 2010) Alkis, I.; Sendag, S.; Oto, G.; Yildirim, S.; Wehrend, A.
Effect of Exercise on Erythrocyte Osmotic Fragility in High Fat Diet Fed Rats During Intermittent Fasting
(Yuzuncu Yil Universitesi Tip Fakultesi, 2017) Yaşar, S.; Günbatar, N.; Yüksek, S.; Arıhan, O.; Oto, G.
Exercise is an important component of a healthy lifestyle. High fat diets are blamed for various health problems such as cardiovascular diseases. Intermittent fasting is a way of increasing longevity and decreasing certain adverse health conditions. In this study effect of exercise, intermittent fasting and high fat diet on erythrocyte osmotic fragility which is an important marker of erythrocyte integrity was investigated. 42 Wistar albino male rats were used in this study. Rats were divided into 7 groups in which each were composed of 6 animals. Exercise was administered 3 times a week. Intermittent fasting was administered 2 times a week (24 without any food in those two days). High fat was given in a high fat diet. Combination of applications was also administered in different groups. Results state an increase in erythrocyte osmotic fragility due to exercise. High fat or intermittent fasting did not cause in increase in fragility however they did not alleviate increased fragility due to exercise where they are concomitantly administered with exercise. Intermittent fasting administered solely or with high fat did not change erythrocyte osmotic fragility therefore stating no negative impact of intermittent fasting on this studied parameter. However no protective effect was observed in IF+Exe group. This result can also be interpreted with high impact of Exe in this study on the rats. Studies including molecular mechanism focusing on erythrocyte membrane lipid alterations should be performed to clarify current findings. © 2017, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effect of Nettle Herb on the Levels of Trace Elements in Rabbits Induced With Dimethylbenzanthracene
(Asian Journal of Chemistry, 2011) Celikezen, F. C.; Ertekin, A.; Turel, I.; Oto, G.
In this study, the effects of nettle herb on the levels of trace elements in rabbits induced with 7,12-dimethylbenzanthracene were studied. Twenty one female New Zealand rabbits were used in the study. The rabbits were divided into three groups. Experiment period was designated as 150 days. The dimethyl sulfoxide (10%) solution with the dosage of 0.5 mL/kg/day was applied in the control group and 7,12-dimethylbenzanthracene dissolved in dimethyl sulfoxide (10 %) solution was applied as 0.5 mL/kg/day in the 7,12-dimethylbenzanthracene group. In addition to 7,12-dimethylbenzanthracene, the extract of nettle herb was applied as 0.2 mL/kg/day to the other group. The blood samples were taken in the end of the experiment. It was determined that there were varying decreases in all tissues of zinc and manganese levels in the 7,12-dimethylbenzanthracene applied group in comparison with the control data, whereas there were increases in all tissues of iron and copper levels. On the other hand, while zinc concentration decreased in all tissues in the nettle herb + 7,12-dimethylbenzanthracene group, manganese, iron and copper concentrations showed an increase in all tissues.
The Effect of Resveratrol Therapy on The Vascular Responses Caused by Chronic Fluorosis
(int Soc Fluoride Research, 2020) Bulduk, M.; Oto, G.; Ozdemir, H.; Demirel-Yilmaz, E.
The aim of this study was to investigate the effect of resveratrol therapy on the contraction-relaxation responses of the thoracic aorta rings and on the blood pressure of rats exposed to chronic fluorosis. The study was conducted using 80 male and female Sprague Dawley rats weighing 200-250 g. The rats were randomly divided into 8 same gender groups of 10 (4 groups of male rats and 4 of female rats) and administered, for each gender via the drinking water, (i) 0 mg/L of sodium fluoride (NaF) and 0 mg/L of resveratrol (control groups); (ii) 10 mg/L of NaF; (iii) 50 mg/L of resveratrol; and (iv) 10 mg/L of NaF and 50 mg/L of resveratrol. Blood pressures were measured on days 30, 60, and 90 in each group. At the end of day 90, the contraction-relaxation responses of the thoracic aorta rings were examined in an isolated organ bath. The analysis on the serum fluorine level revealed that the serum fluorine level was elevated in all the rats administered NaF. For each gender, the most marked elevations in the blood pressures were seen, on all three days, in the NaF groups. In both the male and female groups, the chronic administration of resveratrol with NaF led to decreased blood pressures. The contraction response resulting from phenylephrine administration was increased in the groups administered NaF, whereas it was decreased in the groups administered NaF+resveratrol. In the groups administered resveratrol, the maximal relaxing effect was achieved by acetylcholine. In the groups administered NaF, the relaxation responses caused by SNP (sodium nitroprusside) were similar to those in the other study groups. The results of this study indicate that resveratrol provides a protective effect against the increased blood pressure caused by NaF and the potential endothelial damage. The protective effect of resveratrol results from its capability to reduce fluorine-induced oxidative stress and endothelial tissue damage. Further studies are warranted to investigate the adverse effects in the veins exposed to chronic fluorosis and the molecular mechanisms of the potential protective effects of resveratrol against these adverse effects.
Effect of Safran, Safranal and Crocin Which Are Active Ingredients of Saffron (Crocus) on Erythrocyte Fragility and Hematological Parameters in Carbon Tetrachloride Intoxicated Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Arihan, O.; Oto, G.; Bayram, I.; Aras, I.
Carbon tetrachloride is a toxic chemical and well known for its carcinogenic property. Intoxication with carbon tetrachloride causes serious liver injury and it is used as an experimental model for triggering liver related diseases. Saff ron (Crocus) species are well known bulbous ornamental and aromatic plants. Many of the saffron species are used in ethnomedicinal practices. In addition both saffron species and their ingredients are also subject to scientific research. In this study 72 rats were divided into 9 groups (n=8 in each group). Groups were set as control, olive oil, carbon tetrachloride, safran, safranal, crocin, carbon tetrachloride + safranal, carbon tetrachloride + crocin. Administrations were conducted for 7 days. Erythrocyte osmotic fragility and some selected hematological parameters were assessed. Results state significant increase in erythrocyte fragility due to carbon tetrachloride and amelioration due to active ingredients of saffron. Among hematocrit, hemoglobin and red blood cell count parameters only significant alteration was observed in safran with an increase in red blood cell count. Different administration schemes can be evaluated in future studies in order to assess detailed impact of saffron constituents on hematological parame ters and erythrocyte integrity. © 2017 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effects of Chronically Exposure To Flor and 7,12-Dimethylbenzanthracene (Dmba) on Spermatogenesis and Testicular Histopathology in Rats
(Ataturk Universitesi, 2018) Yildirim, S.; Belhan, S.; Uyar, H.; Huyut, Z.; Oto, G.; Sağlam, Y.S.
This study aimed to investigate the effects of both sodium fluoride (NaF) and polycyclic aromatic hydrocarbon (PAH) derivative such as 7,12-dimethylbenz[a]anthracene (DMBA) singly and in combination on spermatogenesis and testis histopathology in rats. Forty male Wistar albino rats weighing 250–300 g used. On the 90th day of the experiment, intracardiac blood samples and testes were taken. While there was no change in FSH and LH levels in the groups, testosterone levels were significantly decreased in the other groups according to the control group (P<0.001). It was decreased the ratio of motility of sperm, sperm density and, increased the abnormal sperm rate by chronic fluorosis and DMBA toxicity in rats significantly. In histopathological examinations; A decrease in the number of spermatocytes and spermatozoa in the tubulus wall of the testes of rats in the NaF (30 ppm) group was observed. When testicular tissues of rats in the DMBA (10 mg/kg) group were examined, atrophy in tubulus, edema in intertubular spaces and a decrease in the number of spermatocytes in the tubulus wall were found. When testes tissues of rats in the group of NaF (30 ppm) + DMBA (10 mg/kg) were examined, atrophy in seminiferous tubules, severe degenerative and necrotic changes in Spermatogoniums on the walls of tubules and thinning in tubule walls were observed. There is not found spermatozoon in the tubul lumens. Severe edema in the intertubular spaces, dilatation and hyperemia in the veins found in these regions. As a result, it was observed that NaF and DMBA had a negative effect on testicular tissue and fertility singly, and in combination, this effect was further exacerbated. © 2018 Ataturk Universitesi.All Rights Reserved.
Effects of Curcumin Administration on Nesfatin-1 Levels in Blood, Brain and Fat Tissues of Diabetic Rats
(verduci Publisher, 2021) Algul, S.; Ozcelik, O.; Oto, G.; Sarikaya, M.; Goceroglu, R. T.; Embiyaoglu, N. M.; Akcan, A. G.
OBJECTIVE: We evaluated the efficacy of curcumin administration on blood glucose levels and its relationship with nesfatin-1 levels in blood brain and adipose tissue of streptozotocin-induced diabetic rats. MATERIALS AND METHODS: A total of 28 male rats were divided into four groups: control group, type 2 diabetes mellitus (DM) group, control plus curcumin group and type 2DM plus curcumin group. After fifteen days, blood samples were collected from sacrificed rats. Nesftain-1 levels were analysed from blood, brain, and fat tissues of rats in all groups. RESULTS: Nesfatin-1 level was found to be significantly lower in blood, brain and fat tissues of type 2 DM rats compared to the control group. A significant decrease in fasting blood glucose levels was observed in the curcumin administration group compared to type 2 DM group. Improvement of fasting blood glucose level was accompanied by improvement of nesfatin-1 levels in blood, brain, and fat tissues. CONCLUSIONS: As expected, curcumin administration caused significant improvement in fasting blood glucose levels. However, for the first time, we found marked improvements in nesfatin-1 levels in blood, brain, and fat tissues of type 2 DM rats. Thus, considering the crucial role of nesfatin-1 in regulation of glucose metabolism, it is logical to expect an interactive relationship between curcumin and nesfatin-1.
The Effects of Different Types of Antioxidants (Se, Vitamin E and Carotenoids) in Broiler Diets on the Growth Performance, Skin Pigmentation and Liver and Plasma Antioxidant Concentrations
(Facta-fundacio Arnco Ciencia Tecnologia Avicolas, 2016) Karadas, F.; Erdogan, S.; Kor, D.; Oto, G.; Uluman, M.
This study investigated the effects of the addition of different antioxidants to broiler diets on their live performance, liver antioxidant composition and concentrations, immune response, and meat and skin color. A total of 945 three-day-old Ross 308 broiler chicks of both genders were randomly allocated to one of nine dietary treatments (n=105), with three replicates 35 chicks per pen, as follows: T1: control (commercially available corn-and soybean-based broiler diet); T2: selenium (control+0.5 mg/kg Sel-PlexTMSe yeast); T3: vitamin E (control+200 mg/kg Kavimix-E-50 a-tocopherol acetate); T4: lutein (control+100 mg/kg 5% Lutein Beads XB); T5: lycopene (control+100 mg/kg 5% Lyco Beads XB); T6: canthaxanthin (control+25 mg/kg 10% Carophyll (R) Red); T7: apoester (control+25 mg/kg 10% Carophyll (R) Yellow); T8: lutein+zeaxanthin (control+25 mg/kg Xamacol (R)); and T9: beta-carotene (control+100 mg/kg 10% Rovimix (R)). Feed (starter, grower, developer and finisher phases) and water were provided ad libitum for 42 days. Body weights, feed intake, feed conversion values and plasma carotene concentrations were recorded weekly, and liver antioxidant concentrations were recorded at the end of the experiment. Newcastle disease (LaSota) vaccination was performed on day 22. HI titers were measured on days 14, 21, 35 and 42 to determine the effects of the antioxidants on the immune system. The addition of selenium, vitamin E, and carotenoid supplements to the commercial broiler diet significantly increased antioxidant accumulation in the liver and the plasma. All antioxidants assessed significantly improved the immune response. Selenium and vitamin E supplementation also significantly improved total carotenoid concentrations in the plasma. The carotenoids enhanced skin and meat color. None of the supplements tested influenced growth (p>0.05).
How Long-Term Intake of Sodium Fluoride (Naf) in Different Doses and 7,12 Dimethylbenz(A)anhtracene (Dmba) Affect the Erythrocyte Parameters in Rats
(Pakistan Agricultural Scientists Forum, 2019) Comba, B.; Oto, G.; Arihan, O.; Comba, A.; Uyar, H.
This study was aimed to search the effect of Sodium Fluoride (NaF) and 7,12 dimethylbenz(a)anthracene (DMBA) to erythrocyte fragility and parameters in rats. The nine groups were formed and each group contained 8 animals. Group 1: Control group (without any treatment). Group 2: Sesame oil (vehicle for DMBA). Group 3: 1 ppm NaF. Group 4: 15 ppm NaF. Group 5: 30 ppm NaF. Group 6: DMBA.Group 7: 1 ppm NaF + DMBA. Group 8: 15 ppm NaF + DMBA. Group 9: 30 ppm NaF + DMBA. Fluoride was added into the animals' drinking water in the form of NaF once a day, for 12 weeks. DMBA (10 mg/kg) was administered once a week and in a total of 12 weeks with oral gavage. Erythrocyte fragility was analyzed with osmotic hemolysis method and erythrocyte parameters with blood cell counter in whole blood. At 0.4% NaCl concentration groups 4, 5, 6, 8, and 9 showed significantly higher erythrocyte fragility values than control group (p <= 0.05). At 0.5% NaCl concentration, groups 6, 8 and 9 showed significant increase in erythrocyte fragility compared to other groups (p <= 0.05). The erythrocyte and hematocrit values were found significantly high in group 5 (p<0.001) and group 4 (p<0.01) while it was found low in all groups with DMBA (6, 7, 8, 9) (p <= 0.05) compared to control group. Hemoglobin amount in group 5(p <= 0.01) and group 4 (p <= 0.05) were significantly higher than other groups. MCV and MCH in group 5 were significantly lower and these values in all groups with DMBA (6, 7, 8, 9) were determined significantly high compared to other groups. RDWC in group 5 (p <= 0.001) and group 4 (p <= 0.01) and in all groups with DMBA (6, 7, 8, 9) (p <= 0.05) was significantly increased compared to other groups. As a result, exposure to high doses of floride and DMBA may cause augmented erythrocyte fragility, abnormal erythrocyte parameters and anemia. Therefore, measures must be taken to protect the health of all living organisms in area exposed to high levels of fluoride and DMBA.
Investigation of Total Flavonoid, Dpph Radical Scavenging, Lipid Peroxidation and Antimicrobial Activity of Allium Schoenoprasum L. Plant Growing in Van Region
(Centenary University, 2020) Koçak, Y.; Oto, G.; Meydan, İ.; Şeçkin, H.
This study, the effect of ethanol extract of Allium schoenoprasum plant was investigated on some antioxidant parameters and pathogenic microorganisms that affect human health. In this coverage, DPPH (1,1-diphenyl-2-picrilhydrazyl) method was used to determine the antioxidant effect of A. schoenoprasum ethanol extract. Lipid peroxidation prevention activity was evaluated by TBA (Thiobarbituric acid) method. Besides, the antimicrobial activity of plant extract on some pathogenic bacteria was investigated via disk diffusion method. Quercetin was used to determine total flavonoid components. According to our findings, the total flavonoid component amount found in 0.2 mg/ml ethanol extract was found equivalent to quercetin with 44.465 ± 0.204 µg/ml. In addition, A. schoenoprasum showed that it was effective compared to BHA (Beta hydroxy acid), which is the positive control of lipid peroxidation activity. While the lowest and highest concentration values of DPPH radical Scavenging activity were 60,125 - 96,285%, these values were found to be 82.178 - 97.357% for BHA. Ethanol extract of A. schoenoprasum, was determined to show antimicrobial activity against some pathogenic microorganisms such as Bacillus subtilis, Escherichiacoli, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans. © 2020, Centenary University. All rights reserved.
Protective Effect of Borax and Boric Acid on Total Sialic Acid and Lipid-Bound Sialic Acid Levels Against 3-Methylcholanthrene and Benzo(A)pyrene Induced Oxidative Stress in Rats
(Wiley-blackwell, 2016) Ekin, S.; Oto, G.; Gok, F.; Karakus, Y.; Yildiz, D.
Protective Effect of Ferulago Angulata (Schlecht.) Boiss on N-Nitrosodimethylamine Induced Oxidative Stress in Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2019) Kızıltaş, H.; Ekin, S.; Akkoyun, M.B.; Oto, G.; Akbaş, E.
The aim of the present study was to investigate the effect of Ferulago angulata (Schlecht.) Boiss. (FASB) extract on mineral (Ca, K, Na) and trace element (Cu, Zn, Se, Co, Fe, Mn, Cr, Pb, Ni, Cd) levels were determined in rat heart tissue, which was oxidatively stressed with N-nitrosodimethylamine (NDMA). Experimental period was continued with Wistar-Albino rats (n:60) in 21 days. Rats were treated with NDMA (10 mg/kg) in first seven days. FASB plant extract was given to the rats each day of the study. The rats were divided into six groups of ten rats each. The mineral and trace element levels of heart tissue were analyzed by using ICP-OES. The results show that NDMA group was significantly lower results than control group with regarding to Zn, Co and Na levels (p<0.05, p<0.01 and p<0.05). On the other hand the NDMA group was also significantly lower results than the FASB (300 mg/kg) group regarding Zn and Co levels (p<0.05 and p<0.05), whereas the FASB (150 mg/kg) group had increased level of Na according to NDMA group (p<0.05). In conclusion, due to the positive effect on significantly alteration in Zn and Co level of heart tissue, it was suggested that the FASB (150, 3 00 mg/kg) can be used in preventing hearth damage. © 2019, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
The Protective Role of Resveratrol on Serum Total Sialic Acid and Lipid-Bound Sialic Acid in Female Rats With Chronic Fluorosis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Oto, G.; Ekin, S.; Özdemir, H.; Bulduk, M.; Uyar, H.; Öksüz, E.
In the present study, the effect of resveratrol on serum total sialic acid (TSA) and lipid bound sialic acid (LSA) was investigated in the rats exposed to chronic fluoride. The study was administered using 32 female Sprague Dawley rats weighing 200-250 g. Rats were divided into four groups (n=8/group). Group I comprised the control group, group II was treated with sodium fluoride (NaF) (10 mg/L/day), group III was treated with resveratrol (50 mg/L/day) and group IV was treated with NaF+resveratrol for 90 days period. Total sialic acid (TSA) and lipid-bound sialic acid (LSA) were determined in serum samples. Statistical analysis showed that the NaF group was significantly higher than the control group with regards to LSA (17.59±2.734 mg/dL, 12.61±2.013 mg/dL) and TSA (87.86± 8.34 mg/dL, 71.47± 8.57 mg/dL) levels (p<0.01 and p<0.05 respectively). Whereas the Resveratrol group was also significantly lower than the NaF group regarding LSA (13.21±2.848 mg/dL, 17.59±2.734 mg/dL) and TSA (72.44± 10.43 mg/dL, 87.86± 8.34 mg/dL) levels (p<0.05 and p<0.05 respectively), Moreover, no significant differences in LSA (14.62±1.85 mg/dL, 12.61±2.013 mg/dL) and TSA (81.19 ±10.24 mg/dL, 71.47± 8.57 mg/dL) levels were observed in the Resveratrol + NaF groups, as compared to the control group (p>0.05). The present study demonstrated slight positive and beneficial effect of resveratrol on the concentration levels of LSA and TSA in serum. © 2017 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Saffron and Saffron Ingredients Like Safranal and Crocin’s Cytoprotective Effects on Carbon Tetrachloride Induced Liver Damage
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Aras, I.; Bayram, I.; Oto, G.; Erten, R.; Almali, A.Ö.; Ilik, Z.A.
This study aims to investigate the liver-protective effects of saffron and saffron components like crocin and safranal against acute liver injury induced by carbon tetrachloride (CCI4) in rats. 72 male Wistar Albino rats were used in the study. The rats were divided into nine groups: 8 in each group. The rats were sacrificed at the end of the seventh day with high excretion (exsanguina tion). The protective effect of saffron, safranal, and crocin against CCI4-induced acute liver injury was evaluated histopathologically. In the study, balloon degeneration, apoptotic cells, and sub-massive necrosis were seen in hepatocytes as a result of-the effect of CCI4. These histopathological changes were hardly seen in groups in which CCI4 was administered with crocin and saffron. On the other hand, in the group where safranal and CCI4 were given together, changes similar to the histopathological changes seen in the CCI4 group were observed. It was concluded that crocin and saffron have a hepatoprotective effect in the experimental ac ute liver injury caused by CCI4, but the safranal has no protective effect on the liver. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.