Browsing by Author "Oto, Goekhan"

Now showing 1 - 2 of 2

The Effects of Nettle Herb (Urtica Dioica L.) on the Levels of Trace Elements in the Rabbit Fur Treaties With 7,12-Dimetylbenzanthracene
(Kafkas Univ, veteriner Fakultesi dergisi, 2009) Tuerel, Idris; Ertekin, Ali; Oto, Goekhan; Celikezen, F. Caglar; Yasar, Semih
In this study the effect of nettle herb (Urtica dioica L.) on the levels of trace element (Zn, Cu, Mn, Fe) in the rabbit fur treated with 7,12-dimetylbenzanthracene (DMBA) were investigated. The rabbit were divided into three experimental groups and one control group, each group contained seven rabbits. DMBA (0.5 ml/kg/day) were administreated IM to group A as carcinogenic compound. DMBA (0.5 ml/kg/day) were administreated IM as carcinogenic compound plus methanol extract of nettle herb (0.2 ml/kg/day) to group B, DMBA (0.5 ml/kg/day) were administreated IM as carcinogenic compound plus water extract of nettle herb (0.5 ml/kg/day) to group C and 10% dimethylsulphoxide (0.5 ml/kg/day) dissolved in normal saline solution were administrated to control group. All of the treatments were performed for five mounths to each group. The decrases in Cu levels were significant when compared with that of control group (P < 0.01). The decrases in Fe levels in water extract of DMBA+Urtica dioica L. was statistically significant compared with that of control group (P < 0.05). In conclusion. fur trace element levels of groups treated with methanol or water extract of Urtica dioica L. were less compared with that of control group but were higher compared with that of DMBA group. It was thought that Urtica dioica L. can have protective effect against damage caused by DMBA.
Simultaneous Determination of Retinol, Retinyl Palmitate and Β-Carotene in Rat Serum Treated With 7,12-Dimethylbenz[a]anthracene and Hypericum Perforatum L. by High-Performance Liquid Chromatography With Diode-Array Detection
(de Gruyter Poland Sp Z O O, 2010) Levent, Abdulkadir; Ekin, Suat; Oto, Goekhan
A new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and beta-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a] anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min(-1) and 40 degrees C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and beta-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and beta-carotene in rat serum. Results of this study demonstrated that at 60(th) day in the 7,12-dimethylbenz[a] anthracene-treated group there was a significant decrease (p<0.001), (p<0,01), (p<0.05) in levels of retinol, retinyl palmitate and vitamin C, respectively compared to the control group levels. A significant decrease (p<0.01) in retinyl palimitate was observed in the 7,12 dimethylbenz[a] anthracene + Hypericum Perforatum L. treated group compared to the control group..