Browsing by Author "Otur, Cigdem"

Now showing 1 - 3 of 3

Cytotoxicity, Apoptotic, Antioxidant and Antimicrobial Activity of Dictyota Dichotoma (Hudson) Jv Lamouroux 1809 Collected From Antalya Coast, Turkiye
(Pleiades Publishing inc, 2025) Arslan, Sevki; Abdelsalam, Amine Hafis; Kizildogan, Aslihan Kurt; Otur, Cigdem; Konus, Metin; Cetin, Dogan; Okudan, Emine Sukran
Current study was purposed to reveal the cytotoxic, apoptotic, antimicrobial and antioxidant potentials of oil extracted from Dictyota dichotoma (Hudson) J.V. Lamouroux 1809 collected from Antalya coast of Turkiye. Dictyota dichotoma oil showed better cytotoxic activity towards two different adenocarcinoma cell lines (Caco-2 (8.83 mu g/mL) and LnCap (10.86 mu g/mL)) compared to HEK293 cells (non-cancerous). Annexin V/PI staining and qPCR studies revealed that oil caused induction of apoptosis. Dictyota dichotoma oil inhibited the growth of Aspergillus niger ATCC 16404 and Enterococcus faecalis ATCC 29212. Nevertheless, the lower antioxidant activity of Dictyota dichotoma oil was determined compared with trolox standard for both DPPH and ABTS assays (4.8 fold less). In conclusion, Dictyota dichotoma oil has a promising potential in medical usage.
A Novel 4h-Chromen Derivative From Marine Streptomyces Ovatisporus S4702t as Potential Antibacterial and Anti-Cancer Agent
(Bentham Science Publ Ltd, 2022) Kurt-Kizildogan, Aslihan; Akarsu, Neslihan; Otur, Cigdem; Kivrak, Arif; Aslan-Ertas, Nevroz; Arslan, Sevki; Sahin, Nevzat
Background: Marine actinomycetes are among indispensable sources of natural bioactive compounds with unique antimicrobial and anti-cancer activities. Objective: Herein, it was aimed to elucidate the bioactive potential of a marine-derived Streptomyces ovatisporus Methods: Streptomyces ovatisporus S4702T was cultured in N-Z Amine broth, and extraction was carried out using different organic solvents. Bioassay-guided purification was followed by chemical characterization using NMR and LC-MS/MS. The compound was then evaluated for its antibacterial, antioxidant and cytotoxic activities. Results: Etyl acetate extracts gave the highest antibacterial activity, and chemical characterization of this extract indicated the formula as C15H29O5N3 and the corresponding possible molecular structure as 4H-chromen-4-one derivative. It was found highly potent against Bacillus subtilis ATCC 6633 (MIC: 0.25 mu g ml-1) and Micrococcus luteus ATCC 9341 (MBC: 0.5 mu g ml-1). It has no remarkable antioxidant activity, but a higher EC50 value and less cytotoxicity against normal cells. The EC50 values of this chromen derivative were found as 9.68 mu g ml-1 for human colon carcinoma, 9.93 mu g ml-1 for human prostate adenocarcinoma and 25.5 mu g ml-1 for human embryonic kidney cells. Conclusion: Overall, the presented 4H-chromen-4-one derivative is a remarkable bioactive compound with potent antibacterial and cytotoxic activity. With its high bioactive potential, it is proposed as a good candidate in medicine.
Synthesis, Cytotoxicity, Antioxidant and Antimicrobial Activity of Indole Based Novel Small Molecules
(Bentham Science Publ Ltd, 2021) Kurt-Kizildogan, Aslihan; Otur, Cigdem; Yilmaz, Can; Arslan, Sevki; Mutlu, Dogukan; Kivrak, Arif; Konus, Metin
Aims: In this study experiments were carried out to explore antioxidant, antimicrobial, cytotoxic properties of novel indole derivative 1-ethyl-2-phenyl-3-phenylethyl-3-thiophen-2-yl-1Hindole (EPI) together with its effect on glutathione S-transferases (GST) activities in human liver carcinoma (HepG2) cells. Background: Indoles probably represent one of the most important heterocyclic structures that have been attracting the interest of many scientists in drug discovery. Objective: The present study was carried out to explore antioxidant, antimicrobial, cytotoxic properties of novel indole derivative 1-ethyl-2-phenyl-3-phenylethyl-3-thiophen-2-yl-1H-indole (EPI) and its effect on glutathione S-transferases (GST) activities in human liver carcinoma (HepG2) cells. Materials and Methods: Pd-catalyst Sonogashira coupling reactions, MTT Assay, Antioxidant capacity test, Antimicrobial test, GST enzyme activity test Results: 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties. It displayed significant induction in glutathione S-transferases (GST) enzyme activity in human liver cancer cell lines (HepG2), but cytotoxic effect on all tested cancer cell lines could not be observed. Conclusion: All of these results showed that 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties without cytotoxic effect, which could make it a promising active component with further studies..