Browsing by Author "Ozarslan, Merve"

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3d-2d Microleakage Assessment of Preheated Bulk-Fill Composite Resin Applied With Different Parameters: a Micro-Ct Analysis
(Springer, 2023) Can, Dilber Bilgili; Ozarslan, Merve
This study evaluated microleakage from class II cavities filled with bulk-fill composite preheated to different temperatures, applied at different thicknesses, and with different polymerization modes. A total of 60 mesio-occlusal cavity were drilled into the extracted human third molars at 2 mm and 4 mm thickness. Preheated bulk-fill composite resin (Viscalor; VOCO, Germany) was applied to the cavities at 68 degrees C and 37 degrees C after the adhesive resin was applied, and cured using standard and high-power light-curing modes of a VALO light-curing unit. An incrementally applied microhybrid composite was used as the control. The teeth were subjected to 2000 cycles of heating to 55 degrees C and cooling to 5 degrees C with a 30-s hold time. Then, they were immersed in a 50% silver nitrate solution for 24 h and scanned with micro-computed tomography. Scanned data were processed using the CTAn software. Two (2D) and three (3D) dimensional analyses of leached silver nitrate were performed. The data's normality was assessed using the Shapiro-Wilk test before comparisons using a three-way analysis of variance. In both 2D and 3D analysis, bulk-fill composite resin preheated to 68 degrees C and applied at 2 mm thickness showed less microleakage. In the 3D analysis, restorations applied at 37 degrees C and 4 mm thickness in high-power mode showed significantly higher values (p < 0.001). Preheated bulk-fill composite resin can be applied at 68 degrees C and effectively cured at both 2 mm and 4 mm thickness.
Biofilm Formation of C. Albicans on Occlusal Device Materials and Antibiofilm Effects of Chitosan and Eugenol
(Mosby-elsevier, 2024) Ozarslan, Merve; Avcioglu, Nermin Hande; Can, Dilber Bilgili; Caliskan, Ahmet
Statement of problem. Microbial adhesion on occlusal devices may lead to oral diseases such as candidiasis. Whether chitosan and eugenol provide antibiofilm effects is unclear. Purpose. The purpose of this in vitro study was to evaluate the biofilm formation of C. albicans strains on occlusal device materials and the antibiofilm effects of chitosan and eugenol against C. albicans on these surfaces. Material and methods. A total of 88 specimens (5x10x2 mm) were produced from occlusal device materials with 4 production techniques: vacuum-formed thermoplastic (Group V), head-press (Group H), computer-aided design and computer-aided manufacture (CAD-CAM) (Group C), and 3-dimensionally (3D) printed (Group D) (n=22). After various finishing procedures, the surface properties of the specimens were evaluated by using surface free energy (SFE), surface roughness (SR) measurements, and elemental and topographic analysis. Biofilm formation of C. albicans strain and the antibiofilm effects of chitosan and eugenol against biofilm formation on these surfaces were also examined with a crystal violet assay. The distribution's normality was statistically analyzed with the Kolmogorov-Smirnov test. One-way and two-way analysis of variance with post hoc Tukey tests were used for statistical evaluations (alpha=.05). Results. Surface roughness values in Groups D and H were significantly higher than in other groups (P.05). While the highest surface free energy values (except gamma p) were in Group V, Group C had the highest gamma p. The lowest biofilm value appeared in Group H. Chitosan exhibited an antibiofilm effect in all groups except Group H, while eugenol was effective in all groups. Conclusions. The production method affected the susceptibility of occlusal device materials to the adhesion of C. albicans. Eugenol was an effective antibiofilm agent for device materials. (J Prosthet Dent 2024;131:144.e1 -e9)
Effect of Different Polishing Techniques on Surface Properties and Bacterial Adhesion on Resin-Ceramic Cad/Cam Materials
(Springer Heidelberg, 2022) Ozarslan, Merve; Bilgili Can, Dilber; Avcioglu, Nermin Hande; Caliskan, Secil
Objectives The aim of this study is to evaluate the adhesion of Streptococcus mutans on the surface of CAD/CAM materials with various surface treatments. Methods Vita Enamic, Lava Ultimate, and Cerasmart materials were used in this study. A total of 90 samples were prepared (n=10). After various finishing and polishing procedures (non-polished, manual polished, and glazed), surface roughness (SR) measurements, surface free energy (SFE), and elemental and topographic analysis with FIB-SEM/EDX were used to evaluate the samples' surface properties. To improve bacterial adhesion, CAD/CAM materials were covered with sterile artificial saliva containing mucin for pellicle formation and incubated for 1 h at 37 degrees C. Bacteria were then inoculated into the pellicle-coated specimens, and incubation was performed at 37 degrees C for 24 h. Bacterial adhesion was determined as x 10(5) CFU/mL and monitored using FIB-SEM analysis. The Kolmogorov-Smirnov test was used to statistically analyze the normality of the distribution; the groups were then compared using one-way ANOVA and Tukey's test. Results The SR of the control group was statistically higher in all materials (p < 0.05). There were no statistically significant differences in SR between all materials in the non-polished and manual polished groups (p > 0.05). The Vita Enamic control group exhibited the highest SFE value. The highest S. mutans adhesion was observed in non-polished (p< 0.05). Vita Enamic samples had higher CFU than other groups. Conclusions Non-polished surfaces showed higher SR and bacterial adhesion. Polishing processes affected the surface properties and bacterial adhesion.
Evaluation of Color Stability and Microhardness of Contemporary Bulk-Fill Composite Resins With Different Polymerization Properties
(Wiley, 2022) Can, Dilber Bilgili; Ozarslan, Merve
Objectives To determine the color stability and microhardness of three bulk-fill composites with different polymerization characteristics, after immersion in four different solutions. Materials and Methods Color measurements of three bulk fill (Viscalor (VIS), Tetric PowerFill (TPF), Fill Up! (FUP)) and a microhyrid composite resin (G-aenial posterior [GCP]) were performed after polymerization. The specimens were immersed in coffee, cola, red wine and distilled water. Discolorations were recorded after 24 h (T1), 10 days (T2) and 30 days (T3) of immersion. Delta E (CIEDE2000) values were calculated. Vickers microhardness (VHN) was measured from top and bottom surfaces at T0 and T3. Data was analyzed with Two-way ANOVA, One-way ANOVA and Tukey post hoc tests. Results The highest discoloration was seen in wine and the lowest in distilled water and cola. At T3, there was no difference in distilled water groups, while the statistically highest Delta E-00 were obtained in TPF and GCP in coffee, cola and wine. VIS groups had the highest VHN values at T0 and T3 in all solutions. Conclusions Samples immersed in coffee and wine showed discoloration beyond clinically acceptable limits. After 30 days, discoloration occurred in all materials immersed in all solutions. At T3, VIS microhardness generally decreased, while TPF did not. Clinical Significance After 30 days, all bulk-fill composites immersed in distilled water, coffee and wine showed clinically unacceptable Delta E-00.