Browsing by Author "Ozbek, H"

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Anti-Fibrogenic Effects of Captopril and Candesartan Cilexetil on the Hepatic Fibrosis Development in Rat - the Effect of At1-R Blocker on the Hepatic Fibrosis
(Elsevier Gmbh, 2003) Tuncer, I; Ozbek, H; Ugras, S; Bayram, I
Background/Aim: Angiotensin converting enzyme (ACE) and angiotensin II (AT-II) have been suggested to play an important role in liver fibrogenesis. There is a significant relationship between inheritance of hightened expression of transforming growth factor beta1 (TGF-beta1) and AT-II and the development of progressive hepatic fibrosis. The purpose of this study was to investigate the effects of captopril. an ACE inhibitor and candesartan cilexetil, an AT-II type I receptor (AT1-R) blocker, on liver fibrosis induced in rats by carbon tetrachloride (CCl4) administration. Methods: rats were divided into 4 experimental groups: The first group was given CCl4 alone; the second was given both CCl4 and captopril (100 mg(.)kg(-1.)day(-1)); the third was given both CCl4 and candesartan cilexetil (8 mg(.)kg(-1.)day(-1)); fourth group was given 0.9% NaCl only. Seven weeks after initiating the treatment. indices of fibrosis were assessed. Results: Candesartan cilexetil treatment significantly reduced the fibrosis development. These inhibitory effects were not observed in the captopril-treated group. The mean fibrosis score was significantly lower in the CCl4/candesartan group compared with the group applied to CCl4 alone and the group applied to CCl4/captopril. Similarly, the number of alpha-smooth muscle actin positive cells was markedly suppressed by candesartan treatment. Conclusions: The results suggest that AT-II plays a pivotal role in hepatic fibrogenesis and candesartan significantly attenuates the progression of liver fibrosis. This drug may provide an effective new strategy for prevention of liver fibrosis. Its effectiveness should be investigated in chronic liver disease associated with progressive fibrosis.
The Assessment of Effects of Atracurium and Mivacurium Using Tof Guard Device in Dogs
(indian veterinary Journal, 2003) Tas, A; Aslan, L; Atasoy, N; Ceylan, E; Altug, ME; Ozbek, H
The Comparison of Effects of Atracurium and Mivacurium With Tof Guard Device in Cats
(indian veterinary Journal, 2003) Tas, T; Genccelep, M; Atasoy, N; Ozbek, H; Ceylan, E
The Effect of Colchicine on the Peritoneal Membrane
(Taylor & Francis inc, 2006) Sayarlioglu, H; Dogan, E; Erkoc, R; Ozbek, H; Bayram, I; Sayarlioglu, M; Bozkurt, H
Peritoneal dialysis (PD) is a treatment modality for patients with renal failure. Peritoneal fibrosis is one of the most serious complications after long-term continuous ambulatory peritoneal dialysis (CAPD). Histological studies in both humans and animals show that chronic peritoneal dialysis results in fibrosis of the peritoneal membrane. In our study, we investigated the effect of colchicine on peritoneal alterations induced by hypertonic PD solution in rats. Sprague-Dawley rats intraperitoneally received saline (control group) once daily, for 28 days, or 3.86% glucose (PDF group), or 3.86% glucose plus colchicine (colchicine group). Animals from each group were sacrificed after 28 days with anesthetized ketamine (60 mg/kg BW). For the PD fluid assessment, 1 h before the sacrifice of animals, 10 mL PD fluid of 2.27% glucose was given, and this fluid was obtained after the sacrifice. The levels of transforming endothelial growth factor ss (TGF-ss), tumor necrosis factor alpha (TNF-alpha) and albumin were investigated both in the peritoneal dialysate and blood, and the levels of malondialdehyde (MDA) were investigated only in peritoneal dialysate. The peritoneal membrane was evaluated histologically by light microscopy. When groups were compared in terms of body weight change, the colchicine group significantly lost weight compared to controls and PDF group (-4.7% 4.5, 3.5% 7.2, 3.0% 1.3, respectively, p = 0.018). Also, the blood albumin level was significantly lower for these in the colchicine group compared to those in the PDF group (2.7 0.35 versus 3.2 0.3 g/dL, respectively, p = 0.048). The blood TGF-ss level was significantly lower in the control group, and no difference was observed between the PDF and colchicine groups (294.4 67.5 versus 787.4 237.4 versus 615.3 235.1 pg/mL, respectively, p = 0.004). The mesothelial thickness found in groups was as follows: control group 102 18.9 mu m, PDF group 128.33 33.1 mu m, colchicine group 117 35.6 mu m ( p = 0.34). In conclusion, a rat model for peritoneal dialysis associated peritoneal derangement without fibrosis could be induced. Colchicine could not prevent peritoneal derangement in this model.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats
(Wiley, 2003) Kanter, M; Meral, I; Dede, S; Cemek, M; Ozbek, H; Uygan, I; Gunduz, H
This study was designed to investigate the effects Nigella sativa L. (NS) and Urtica dioica L. (UD) on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in carbon tetrachloride (CCl4)-treated rats. A total of 60 healthy male Sprague-Dawley rats were utilized in this study. The rats were randomly allotted into one of four experimental groups: A (CCl4 -only treated), B (CCl4 + UD treated), C (CCl4 + NS treated) and D (CCl4 + UD + NS treated), each containing 15 animals. All groups received CCl4 [0.8 ml/kg of body weight, subcutaneously, twice a week for 90 days starting day 1]. In addition, B, C and D groups also received daily intraperitoneal injections of 0.2 ml/kg NS or/and 2 ml/kg UD oils for 45 days starting day 46. Group A, on the other hand, received only 2 ml/kg normal saline solution for 45 days starting day 46. Blood samples for the biochemical analysis were taken by cardiac puncture from five randomly chosen rats in each treatment group at beginning, at 45th and at 90th day of the experiment. The CCl4 treatment for 45 days increased the lipid peroxidation and liver enzymes, and also decreased the antioxidant enzyme levels. NS or UD treatments (alone or combination) for 45 days starting day 46 decreased the elevated lipid peroxidation and liver enzyme levels and also increased the reduced antioxidant enzyme levels. Live weights of the rats decreased in group A, and increased in groups B, C and D. It is concluded that NS and UD decrease the lipid peroxidation and liver enzymes, and increase the antioxidant defence system activity in the CCl4-treated rats.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats (Vol 50, Pg 264, 2003)
(Blackwell verlag Gmbh, 2003) Kanter, M; Meral, I; Dede, S; Gunduz, H; Cemek, M; Ozbek, H; Uygan, I
Effects of Nigella Sativa L. on Serum Concentrations of Thyroid Hormones, Thyroid Stimulating Hormone and Glucose in Alloxaninduced Diabetic Rabbits
(veterinary Ireland, 2003) Meral, I; Yener, Z; Ozbek, H; Ustun, R
This study was designed to investigate the effect of an extract of Nigella sativa L. (NS) on the histology and function of the thyroid glands in alloxan-induced diabetic rabbits. Fifteen New Zealand male rabbits were divided into three experimental groups: control, diabetic and NS-treated diabetic. At the start of the experiment, a single intravenous injection of 10% alloxan (150 mg/kg body weight (bwt)) was given to produce diabetes mellitus (DM) in the diabetic and NS-treated diabetic groups. After DM was confirmed in the two groups, rabbits in the NS-treated group were given oral doses of 20 ml/kg bwt of the NS extract every day for two months. Then Mood samples were taken from all 15 rabbits to measure the serum concentrations of glucose, triiodothyronine (T-3), thyroxine (T-4) and thyroid stimulating hormone (TSH). The rabbits were killed and tissue samples were taken from the thyroid glands for histopathological analysis. It was found that NS treatment decreased the elevated glucose concentrations, and raised the lowered serum triiodothyronine concentrations in diabetic rabbits. There were no significant differences between groups in the histology of the thyroid glands or in the concentrations of serum T, and serum TSH. It was suggested that oral NS treatment might decrease the diabetes-induced disturbances of thyroid hormone metabolism.
Effects of Scorpion Envenomation on Blood Constituents in Rats
(indian veterinary Journal, 2004) Sahin, A; Ozbek, H; Dulger, H; Ozturk, G; Dagoglu, G
Effects of Subchronic Exposure To Thiocarbamide on Haematological and Biochemical Constituents of Rats
(Parlar Scientific Publications (p S P), 2004) Celik, I; Ozbek, H
The effects of sublethal concentration of thiocarbamide on various haematological and biochemical constituents of rat were investigated under laboratory conditions. 250 ppm of thiocarbamide was orally administered to eight ad libitum-fed rats during the test period of 25 days. Various haematological and biochemical constituents of the rat were determined after treatment with thiocarbamide, which caused significant increases in lactate dehydrogenase (LDH) and creatine phosphokinase (CPK), while the level of alanine aminotransferase (ALT) was decreased. Aspartate aminotransferase (AST) and amylase level did not change. On the other hand, the treatment of rats with thiocarbamide also resulted in a different effect on the level of blood constituents compared to that of control rats. While the levels of white blood corpuscles (WBC) and thrombocytes (PLT) were increased significantly by thiocarbamide, the other parameters did not change. With regard to the biochemical characteristics, the levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDLC) were increased significantly by thiocarbamide, but the other consituents did not change. It is concluded from the experiments of this study that thiocarbamide may cause toxicity in different tissues/organs of the laboratory in rats.
Effects of Topical Mitomycin on Inner Ear
(Tohoku Univ Medical Press, 2002) Çankaya, H; Egeli, E; Kuntsal, L; Ozbek, H; Icli, M
QANKAYA, H., EGELI, E., KUNTSAL, L., OZBEK, H. and ICLI, M. Effects of Topical Mitomycin on Inner Ear: A Light and Electron Microscopic Study. Tohoku J. Exp. Med., 2002, 197 (2), 81-86 - Providing maintenance of myringotomy patency without use of ventilation tubes in the treatment of secretory otitis media has been one of the important study areas. For this reason, laser and Mitomycin C (MMC) are used together in experimental studies. But there has been no ultrastructural studies concerning whether leakage of MMC to middle ear during application of this procedure has an ototoxic effect or not and if so, to what extent. In this study, we searched the ultrastructural changes which occurred in the middle ear by direct applications of MMC to the middle ear for different time durations. The study was carried out over thirty adult guinea pigs without ear diseases. Bilateral myringotomy was performed and MMC was applied only to the right middle ear of each guinea pig. The first group received MMC once for 10 minutes, the second group received it once for 20 minutes, and the third group took it each day for 10 minutes during a one week period. The left ears of the samples were accepted as the control group. On the 8th day, sacrification was carried out. After electron and light microscopy examination, significant changes in the inner ear were observed in the third group though no significant change was observed for the first and the second groups. As a result it was concluded that the application of MMC to the middle ear once for a short duration causes no toxic effect on the inner ear. - mitomycin; secretory otitis media; myringotomy; ototoxicity; inner ear. (C) 2002 Tohoku University Medical Press.
Hepatoprotective Effect of Pentoxifylline
(indian veterinary Journal, 2005) Bayram, I; Ozbek, H; Ugras, S; Tuncer, Y
Interferon-Beta Treatment in Multiple Sclerosis Patients Decreases the Number of Monocytes in Peripheral Blood
(John Wiley & Sons Ltd, 2003) Anlar, O; Tombul, T; Ozbek, H; Dilek, L
Multiple sclerosis (MS) is a demyelinating disease of the central nervous system, characterized by the presence of sclerotic lesions throughout the brain. During clinical activity, monocytes activation markers such as Interleukin 1 and tumor necrosis factor-alpha (TNF-alpha) increase. The effect of interferon beta (IFN-beta) in reducing exacerbation rate and in slowing sustained worsening of disability has been shown in MS. The aim of this study was to determine levels of monocytes percentage in peripheral blood of MS patients receiving IFN-beta. We carried out this study in two groups of MS patients and a group of healthy subjects. First (study) group consisted of 13 patients treated by INF-beta. The control groups consisted of a) 14 MS patients group with no treatment by INF- beta and b) 20 healthy subjects. We counted the percentage of monocytes in the study group before and after one year of treatment by IFN- beta and in the control groups patients in the same period. At the end of one year, there was a significant reduction in the percentage of monocytes in peripheral blood of MS patients treated by interferon.
A New Agent for Treatment of Acute Respiratory Distress Syndrome: Thymoquinone. an Experimental Study in a Rat Model
(Oxford Univ Press inc, 2005) Isik, AF; Kati, I; Bayram, I; Ozbek, H
Objective: Acute respiratory distress syndrome (ARDS) and acute lung injury (ALI) are important clinical problems in thoracic surgery and critical care medicine. Most of the treatment methods are still supportive. Thymoquinone has anti-inflammatory, spasmolytic, bronchoditator and antibacterial effects. We studied its effects on ALI/ARDS in a rat model. Methods: ALI/ARDS was developed in 40 Sprague-Dawley mate rats (200-250 g in weight) by intratracheal instillation of human gastric juice (pH 1.2). Rats were treated with mechanic ventilator for 3 h. There were five groups: Control group (n = 11); Steroid group (n = 10); Ethanol group (n = 5); Thymoquinone group (n = 9) and Thymoquinone+Steroid group (n = 5). No instillation except gastric juice was applied in the first group. Thymoquinone was given in dosage of 6mg/kg, metilprednisolone in dosage of 10 mg/kg, ethanol 0.75 ml/kgintraperitoneatly (IP). Blood gas analysis and compliance measurement were done. At the end of the third hour, rats were sacrificed and their lungs were excised for histopathotogical examination. Results: In the thymoquinone group, the ratio of arterial oxygen to the fraction of inspired oxygen (PO2/FiO(2),) was significantly better compared to the other groups (P = 0.0000.043). Static compliance measurements revealed higher values in thymoquinone and thymoquinone+steroid groups. Histopathotogical examinations showed that affected lung tissue is tower in groups 2 and 4 (P = 0.000-0.027). Conclusions: This study revealed that thymoquinone improved oxygenation white both thymoquinone and steroids protect lung tissue from hazardous effects of human gastric juice (pH 1.2) histopathologically. (c) 2005 Elsevier B.V. All rights reserved.
Organochlorine Pesticide Residues in Human Breast Milk From Agricultural Regions of Turkey, 1995-1996
(Springer, 1997) Cok, I; Bilgili, A; Ozdemir, M; Ozbek, H; Bilgili, N; Burgaz, S
Partial Regeneration/Proliferation of the Β-Cells in the Islets of Langerhans by Nigella Sativa L. in Streptozotocin-Induced Diabetic Rats
(Tohoku Univ Medical Press, 2003) Kanter, M; Meral, I; Yener, Z; Ozbek, H; Demir, H
This experiment was carried out to investigate the effect of N. sativa L. on histopathology of pancreatic beta-cells, and blood insulin and glucose concentrations in streptozotocin-induced diabetic rats. Fifty male Wistar rats (200-250 g) were divided into two experimental groups (diabetics with no treatment and diabetics with N. sativa L. treatment), each containing twenty-five rats. Diabetes was induced in both groups by a single intraperitoneal injection of streptozotocin (STZ) (50 mg/kg). The experimental animals in both groups became diabetic within 24 hours after the administration of STZ. The rats in N. sativa L.-treated group were given the daily intraperitoneal injection of 0.20 ml/ kg of A sativa L. volatile oil for 30 days starting the day after STZ injection. Control rats received only the same amount of normal saline solution. The rats in both groups received the last injection 24 hours before the sacrification and 5 randomly-selected rats in each group were sacrificed before, and the 1, 10, 20 and 30 days after the STZ injection to collect blood and pancreatic tissue samples. The N. sativa L. treatment caused a decrease in the elevated serum glucose, an increase in the lowered serum insulin concentrations and partial regeneration/ proliferation of pancreatic beta-cells in STZ-induced diabetic rats with the elapse of the experiment. It is concluded that the hypoglycaemic action of N. sativa L. could be partly due to amelioration in the beta-cells of pancreatic islets causing an increase in insulin secretion. More studies are needed to demonstrate the exact mechanism of action of N. sativa L. on ameliorated blood glucose concentration in STZ-induced diabetes. -rats; N. sativa L.; glucose; insulin, beta-cells. (C) 2003 Tohoku University Medical Press.
The Role of Urtica Dioica and Nigella Sativa in the Prevention of Carbon Tetrachloride-Induced Hepatotoxicity in Rats
(Wiley, 2003) Türkdogan, MK; Ozbek, H; Yener, Z; Tuncer, I; Uygan, I; Ceylan, E
The role of Nigella sativa L. (Ranunculaceae) (NS) and Urtica dioica L. was investigated (UD) in the prevention of carbon tetrachloride (CCl4) induced liver fibrosis and cirrhosis. Fifty Sprague-Dawley rats were allocated into five groups (I, IIA and B, IIIA and B) and CCl4 was injected biweekly to all groups. Group I (control, CCl4 only), group IIA and B (NS fixed oil and volatile oil), group IIIA and B (UD fixed oil and UD decoction extract) rats were killed at the end of week 12 and histopathological and immunohistochemical examinations of liver tissues were performed. In the control group, coagulation necrosis and hydropic degeneration were marked in the periacinar regions (zone 3) associated with fibrosis in the periacinar regions and in the portal tracts. In groups IIA-B and IIIA-B (NS and UD), none of the serious histopathological findings were detected except for sparse coagulation necrosis in the periacinar regions. ASMA-positive perisinusoidal cells with myofibroblastic transformation and lysosomal enzyme activity suggesting fibrogenesis were also significantly more common in the control group than in the NS and UD groups. UD and NS seem to be significantly effective in the prevention of carbon tetrachloride induced hepatotoxicity in rats. Copyright (C) 2003 John Wiley Sons, Ltd.
Visual Evoked Potentials in Multiple Sclerosis Before and After Two Years of Interferon Therapy
(Taylor & Francis Ltd, 2003) Anlar, O; Kisli, M; Tombul, T; Ozbek, H
Magnetic resonance imaging (MRI) is important in the diagnosis of and follow-up for the treatment of multiple sclerosis (MS); evoked potentials may be important if MRI is normal or cannot be performed. We assessed serial visual evoked potentials (VEPs) and cranial MRI in a group of clinically relapsing-remitting multiple sclerosis (N = 15) treated with interferon beta-lb (INFB-1b) and in normal subjects (N = 15). The investigations were done I week before INFB-lb therapy, 1 year later (N = 15), and 2 years later (N = 10). VEPs were abnormal in most of the patients; MRIs were abnormal in all patients. We used P100 latency as an electrophysiological index for the progress of illness. There were significant differences in VEPs between the beginning and ending of the interferon treatment. We concluded that VEPs would be a reliable index for following up the progress of MS under interferon therapy.