Browsing by Author "Ozbek, Hanefi"

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Analgesic and Hepatotoxic Effects of Ononis Spinosa
(Wiley, 2006) Yilmaz, Betul Sever; Ozbek, Hanefi; Citoglu, Gulcin Saltan; Ugras, Serdar; Bayram, Irfan; Erdogan, Ender
The present study investigated the analgesic and hepatoprotective activities of a water extract of Ononis spinosa L. (OS) in mice. Analgesic activity was based on the pain thresholds measured with the tail-flick test before administration at 30, 90 and 150 min. The results were analysed with one-way variance analysis. The extract of Ononis spinosa showed analgesic activity equivalent to aspirin at 30 and 90 min and even higher than aspirin with the 50 mg/kg dose. At a dose of 100 mg/kg OS showed an analgesic effect equivalent to aspirin at all time points. The hepatoprotective influence of OS on carbon tetrachloride (CCl4)-induced acute liver toxicity was also studied. The extract had no significant effect on the increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and bilirubin in CCI4 treated animals (p > 0.05). Thus, the results reveal that the extract of OS had no hepatoprotective effect on CCI4-induced acute liver toxicity. Copyright (c) 2006 John Wiley & Sons, Ltd.
Analgesic Compounds From Scorzonera Latifolia (Fisch and Mey.) Dc
(Elsevier Ireland Ltd, 2010) Bahadir, Ozlem; Citoglu, Guelcin Saltan; Smejkal, Karel; Dall'Acqua, Stefano; Ozbek, Hanefi; Cvacka, Josef; Zemlicka, Milan
Ethnopharmacological relevance: A traditional mastic named yaki sakizi prepared from the roots of Scorzonera latifolia (Fisch. and Mey.) DC. is used as a folk remedy for treatment of pain in Turkish folk medicine. Aim of the study: To isolate and identify the compounds responsible for the antinociceptive activity of S. latifolia using bioassay-guided fractionation. Materials and methods: The methanolic extract of the S. latifolia roots was prepared and subjected to isolation procedures such as solvent-solvent partitioning and column chromatography. Writhing and tail-flick tests were used to determine the antinociceptive activity. Results: The n-hexane fraction of the S. latifolia root methanolic extract showed potent antinociceptive activity in both writhing and tail-flick tests. Three compounds were isolated from n-hexane fraction using bioassay-guided chromatographic purification. Isolated compounds were the triterpene taraxasteryl myristate, taraxasteryl acetate, and fern-7-en-3-beta-one, structures were elucidated by means of MS and NMR techniques. Both taraxasterol derivatives showed promising antinociceptive activity when compared to reference compounds. Conclusion: Results of the present study support the usage of S. (Walla in Turkish folk medicine. Both plant root extract and the isolated compounds showed promising antinociceptive activities. Our results suggested that antinociceptive activity of the plant extract is probably caused by the synergistic interaction of the isolated compounds. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Analysis of Factors Associated With Survival in Advanced Stage Gastric Adenocarcinoma
(Kare Publ, 2005) Alici, Suleyman; Kaya, Serap; Izmirli, Mustafa; Tuncer, Ilyas; Dogan, Ekrem; Ozbek, Hanefi; Sayarlioglu, Hayriye
In this study, 138 patients with high grade gastric adenocarcinoma who were admitted to Yuzuncu Yil University, Medical School, Department of Medical Oncology between September 1999 and April 2002 were retrospectively evaluated in terms of the effects of clinicopathological parameters and treatment approaches on survival by single and multiple variable analyses. Patients histopathologically diagnosed as gastric adenocarcinomas with stage IV M0 (without distant organ metastasis) or stage IV M1 (with distant organ metastasis) were included in the study. Mean age of the patients was 61.1 (34-84), 64.6% male and 35.5% female. The ratio of patients with a performance score of 2-3 was 55%. The BMI was below 20 in 53.6% of the patients. According to histological grading, 41.9% of patients had indifferentiated adenocarcinoma, mucinous cell adenocarcinoma or signet ring cell adenocarcinoma. 84% of patients were clinically at stage IV M1. The percentage of patients with tumor localized upper 1/3 of stomach was 60.8%. While 19.6% of patients had received surgical treatment, 47% of the patients had received chemotherapy as adjuvant or paliative purposes. The percentage of the patients who responded to the therapy (complete response + partial response + stable response) was 39% and the percentage of patients who had progression was 7.2%. Overall median survival time was 3.1 months and three years survival rate was 8%. At single variable analysis, BMI, clinical stage, surgery, type of surgery and serum level of albumin were significant prognostic factors related to overall median survival time. Gender, clinical stage, surgery, type of surgery, hemoglobine concentration and serum level of albumin were found to be significant prognostic factors related to survival without progression (p<0.05). The factors found to be significant related to overall survival rate in single variable analysis were reevaluated in multiple variable analysis. No surgical treatment, paliative surgery (compared to radical surgery), BMI below 20 were found to be the statistically significant poor prognostic factors related to survival. The factors found to be significant related to survival without progression in single variable analysis were evaluated in multiple variable analysis and no surgical treatment (compared to surgery), male gender (compared to female) were found to be statistically significant poor prognostic parameters. In terms of both overall survival and survival without progression, whether performing surgery or not was statistically most significant independent prognostic factor. In conclusion, no surgical treatment, paliative surgery instead of radical surgery, and BMI below 20 on first admission were determined as poor prognostic factors related to survival in patients with high grade gastric adenocarcinoma.
Analysis of Survival Factors in Patients With Advanced-Stage Gastric Adenocarcinoma
(int Scientific information, inc, 2006) Alici, Suleyman; Kaya, Serap; Izmirli, Mustafa; Tuncer, Ilyas; Dogan, Ekrem; Ozbek, Hanefi; Sayariloglu, Hayriye
Background: Prognosis in patients with gastric cancer is determined by the tumor itself, as well as certain patient-related factors. Material/Methods: In this study, 138 patients with high-grade gastric adenocarcinoma who were admitted to our hospital between September 1999 and April 2002 were retrospectively evaluated in terms of the effects of clinicopathological parameters and treatment approaches on survival by single and multiple variable analyses. Patients histopathologically diagnosed as having gastric adenocarcinomas with stage IV M-0 (without distant organ metastasis) or stage IV M-1 (with distant organ metastasis) were included in the study. Results: Overall median survival time was 3.1 months and three-year survival rate was 8%. With single variable analysis, body mass index (BMI), clinical stage, surgery, type of surgery, and serum level of albumin were significant prognostic factors related to overall median survival time. Gender, clinical stage, surgery, type of surgery, hemoglobin concentration, and serum level of albumin were found to be significant prognostic factors related to survival without progression (p < 0.05). No surgical treatment, palliative surgery (compared with radical surgery), and BMI below 20 were found to be the statistically significant poor prognostic factors related to survival in multiple variable analysis. In terms of both overall survival and survival without progression, performing surgery or not was statistically the most significant independent prognostic factor. Conclusions: No surgical treatment, palliative surgery instead of radical surgery, and BMI below 20 on first admission were determined as poor prognostic factors related to survival in patients with high-grade gastric adenocarcinoma.
The Anti-Inflammatory Activity of Eugenia Caryophyllata Essential Oil: an Animal Model of Antiinflammatory Activity
(Modestum Ltd, 2005) Ozturk, Abdurrahman; Ozbek, Hanefi
Aim: The aim of this study is gas chromatographic analysis of Eugenia caryaphyllata (clove) essential oil and investigation of its anti-inflammatory effects. Methods: The study involved eight groups; Serum physiologic, ethyl alcohol, indomethacin (3 mg/kg), etodolac (50 mg/kg), cardamom (0.05 mL/kg), EC-I (0.025 mL/kg), EC-II (0.050 mL/kg), EC-III (0.100 mL/kg) and EC-IV (0.200mL/kg). After measuring the volumes of right hind-paws of rats using a plethysmometer, drugs were injected intraperitoneally and lambda-carrageenan were injected subcutaneously into the plantar region. Three hours after the injections the volume measurements of the right hind-paws were repeated and the difference between the volumes were compared. Results: The composition of the essential oil was as follows: beta-caryophyllen % 44.7, eugenol % 44.2,alpha-humulen % 3.5, eugenyl acetate % 1.3 and alpha-copaen % 1.0. It was found that indomethazin reduced the inflammation by 95.70%, etodolac by 43.42 %, EC-I by 46.55 %, EC-II by 90.15 %, EC-III by 66.94 % and EC-IV by 82.78 %. The essential oil of Eugenia caryophyllata had an anti-inflammatory effect matching to that of etodolac at 0.025 and 0.1 mL/kg and to that of indomethacin at 0.05 and 0.2 mL/kg doses. Conclusion: As a result Eugenia caryophyllata essential oil extract was shown to have an antiinflammatory effect.
Are Nigella Sativa and Urtica Dioica Fixed Oils Effective on Carbon Tetrachloride-Induced Liver Injury Model in Rats
(Studium Press Llc, 2008) Ozbek, Hanefi; Ozturk, Mustafa; Erdogan, Ender; Kosem, Mustafa
Effectiveness of Nigella sativa and Urtica dioica. in prevention of carbon tetrachloride-induced liver injury were investigated in this study. Sprague-Dawley rats were given carbon tetrachloride dissolved in olive oil intraperitoneally three times a week for 11 weeks. After liver fibrosis was developed, carbon tetrachloride was discontinued and rats were randomized in four groups. They were treated with Nigella sativa fixed oil (2 ml/kg), Urtica dioica fixed oil (2 ml/kg) or Nigella sativa (2 ml/kg) and Urtica dioica (2 ml/kg) per orally for four weeks. The control group was untreated. The animals were sacrificed and specimens were taken for biochemical and histopathological study at the end. Cessation of carbon tetrachloride allowed for partial recovery of liver tissue by autoregeneration in control group. Liver fibrosis was further progressed in all three treatment groups and resulted in cirrhosis in some animals. In Urtica dioica group alanine aminotransferase level was significantly higher compared to other groups. Serum indirect bilirubin levels were higher in Urtica dioica and Nigella sativa groups compared to control and Nigella sativa+Urtica dioica groups. Present study indicate that fixed oils of Nigella sativa, Urtica dioica and Nigella sativa +Urtica dioica did not show a therapeutic effect in experimental liver fibrosis in rats.
The Effect of Nigella Sativa Oil Against Aspiculuris Tetraptera and Hymenolepis Nana in Naturally Infected Mice
(Saudi Med J, 2007) Ayaz, Erol; Yilmaz, Hasan; Ozbek, Hanefi; Tas, Zeynep; Orunc, Ozlem
Objectives: To investigate the antiparasitic effect of Nigella sativa oil (NSO) on Aspiculuris tetraptera (A. tetraptera) and Hymenolepis nana (H.nana) in mice in January 2005. Methods: Mice were obtained from the animal house facility of the Faculty of Medicine, Yuzuncu Yil University, Van, Turkey. The natural infections were determined by the cellophane tape method and the centrifugal flotation method of stool samples. The infected mice with A. tetraptera and H. nana were divided into 4 groups; 2 treatment and 2 control groups. Nigella sativa oil was given at the dose of 250 mu l/kg body weight orally for 2 consecutive days in the 2 treatment groups. All the mice were sacrificed on the seventh day after the last treatment. Gastrointestinal tract of the sacrificed animals was opened and washed with a serum physiologic. The contents were examined under a stereo microscope for counting and identifying of the parasites. The treatment and the control groups were compared using Mann-Whitney U-Test. Results: Nigella sativa oil reduced both A. tetraptera and its eggs. The difference was significant between Group 1 and Group 3 (p < 0.05). Nigella sativa oil reduced H.nana eggs starting from second day of the treatment until necropsy day during 5 days, but it was not significant between Group 2 and Group 4 (p > 0.05). Conclusions: Antiparasitic effect of NSO is related to its stimulating immune system.
Effects of High-Cholesterol Diet and Antioxidant Vitamins Combination on Oxidant/Antioxidant Status in Heart, Kidney, Liver and Testis Tissues From Rats
(Wiley, 2007) Devrim, Erdinc; Ozbek, Hanefi; Durak, Ilker
Possible effects of a high-cholesterol diet on the oxidant/antioxidant status in various tissues from rats and the effects of the combination of vitamins C and E on these parameters and on hypercholesterolemia were investigated. Twenty-four Sprague-Dawley type rats were randomly divided into three groups. The control group (group 1) was fed on a rat pellet diet. The study groups (groups 2 and 3) were fed on a high-cholesterol diet for 3 months. Rats in group 3 were supplemented with vitamins C (100 mg/kg intraperitoneally ) and E (60 mg/kg intramuscularly ) three times a week throughout the study. At the end of the study period, all animals were sacrificed and their heart, kidney, liver and testis surgically removed to measure oxidant and antioxidant parameters. Blood samples were also obtained from the animals. We observed that serum total cholesterol levels significantly increased in the study groups after the study period. The increase in the blood cholesterol level in group 3 was, however, significantly lower than that in group 2. As to the oxidant-antioxidant parameters, there were different results among the tissues. In conclusion, our results suggest that high serum cholesterol level does not lead to oxidation reactions in the early phase of hypercholesterolemia, and antioxidant vitamins supplementation increases antioxidant potential values of the tissues and decreases the blood total cholesterol level in the cholesterol-supplemented animals.
Evaluation of Anti-Inflammatory Effect of Zosima Absinthifolia and Deltoin
(Academic Journals, 2010) Bahadir, Oezlem; Citoglu, Guelcin Saltan; Ozbek, Hanefi
In this study, n-Hexane extracts prepared from aerial part of Zosima absinthifolia (ZAA) and the root of Z. absinthifolia (ZAR) (Umbelliferae) as well as deltoin were evaluated for their anti-inflammatory activity using the carrageenan-induced rat paw oedema test. The anti-inflammatory activity of Z. absinthifolia was not found to be significantly different at doses of 25 and 50 mg/kg. However, the aerial part of ZAA at 75 mg/kg, i.p., and Deltoin at 10 mg/kg, i.p. showed a significant reduction with 86.56 and 71.05% respectively in rat paw oedema induced by carrageenan against the reference anti-inflammatory drug indomethacin (3 mg/kg, i.p.) (87%). The ED50 of deltoin, ZAA and ZAR were determined as 5.08, 69.82 and 38.51 mg/kg, respectively. Furthermore, content of deltoin and columbianadin in both ZAA and ZAR were also analyzed by HPLC. Deltoin content of plant was found higher than the columbianadin content in ZAA and ZAR. The present study reveals that the aerial part of Z. absinthifolia possesses promising anti-inflammatory activity in rats.
Gastroprotective Activity of Nigella Sativa L Oil and Its Constituent, Thymoquinone Against Acute Alcohol-Induced Gastric Mucosal Injury in Rats
(Baishideng Publishing Group inc, 2005) Kanter, Mehmet; Demir, Halit; Karakaya, Cengiz; Ozbek, Hanefi
Aim: To evaluate the role of reactive oxygen species in the pathogenesis of acute ethanol-induced gastric mucosal lesions and the effect of Nigella sativa L oil (NS) and its constituent thymoquinone (TQ) in an experimental model. METHODS: Male Wistar albino rats were assigned into 4 groups. Control group was given physiologic saline orally (10 mL/kg body weight) as the vehicle (gavage); ethanol group was administrated 1 mL (per rat) absolute alcohol by gavage; the third and fourth groups were given NS (10 mL/kg body weight) and TQ (10 mg/kg body weight p.o) respectively 1 h prior to alcohol intake. One hour after ethanol administration, stomach tissues were excised for macroscopic examination and biochemical analysis. RESULTS: NS and TQ could protect gastric mucosa against the injurious effect of absolute alcohol and promote ulcer healing as evidenced from the ulcer index (UI) values. NS prevented alcohol-induced increase in thiobarbituric acid-reactive substances (TBARS), an index of lipid peroxidation. NS also increased gastric glutathione content (GSH), enzymatic activities of gastric superoxide dismutase (SOD) and glutathione-S-transferase (GST). Likewise, TQ protected against the ulcerating effect of alcohol and mitigated most of the biochemical adverse effects induced by alcohol in gastric mucosa, but to a lesser extent than NS. Neither NS nor TQ affected catalase activity in gastric tissue. CONCLUSION: Both NS and TQ, particularly NS can partly protect gastric mucosa from acute alcohol-induced mucosal injury, and these gastroprotective effects might be induced, at least partly by their radical scavenging activity. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
Hepatoprotective and Anti-Inflammatory Activities of Plantago Major L
(Wolters Kluwer Medknow Publications, 2009) Turel, Idris; Ozbek, Hanefi; Erten, Remzi; Oner, Ahmet Cihat; Cengiz, Nureddin; Yilmaz, Orhan
Objective: The aim of this study was to investigate anti-inflammatory and hepatoprotective activities of Plantago major L. (PM). Materials and Methods: Anti-inflammatory activity: Control and reference groups were administered isotonic saline solution (ISS) and indomethacin, respectively. Plantago major groups were injected PM in doses of 5 mg/kg (PM-I), 10 mg/kg (PM-II), 20 mg/kg (PM-III) and 25 mg/kg (PM-IV). Before and three hours after the injections, the volume of right hind-paw of rats was measured using a plethysmometer. Hepatoprotective Activity: The hepatotoxicity was induced by carbon tetrachloride (CCl4) administration. Control, CCl4 and reference groups received isotonic saline solution, CCl4 and silibinin, respectively. Plantago major groups received CCl4 (0.8 ml/kg) and PM in doses of 10, 20 and 25 mg/kg, respectively for seven days. Blood samples and liver were collected on the 8th day after the animals were killed. Results: Plantago major had an anti-inflammatory effect matching to that of control group at doses of 20 and 25 mg/kg. It was found that reduction in the inflammation was 90.01% with indomethacin, 3.10% with PM-I, 41.56% with PM-II, 45.87% with PM-III and 49.76% with PM-IV. Median effective dose (ED50) value of PM was found to be 7.507 mg/kg. Plantago major (25 mg/kg) significantly reduced the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels when compared to the CCl4 group. The histopathological findings showed a significant difference between the PM (25 mg/kg) and CCl4 groups. Conclusion: The results showed that PM had a considerable anti-inflammatory and hepatoprotective activities.
Hepatoprotective and Hypoglycemic Activity of Viburnum Lantana L
(Turkish Pharmacists Assoc, 2006) Sever Yilmaz, Betul; Saltan Citolu, Gulcin; Altun, M. Levent; Ozbek, Hanefi; Bayram, Irfan; Cengiz, Nurettin; Altinyay, Cigdem
In the present study we investigated the hepatoprotective effect of the water extract of Viburnum lantana L. (VL) leaves on carbon tetrachloride (CC14)-induced hepatotoxicity in rats, hypoglycemic activity and lethal doses of the sante extract in mice. Biochemical parameters of hepatic damage such as serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and bilirubin concentrations were determined. CCI4 (0.8 mL/kg i.p. for 7 days) treatment increased the serum AST, ALT, ALP and bilirubin levels significantly as compared to controls. Treatment of animals with silibinin (50 mg/kg) + CC14 (0.8 mL/kg i.p.) or VL (100 mg/kg, i.p.) + CC14 (0.8 mL/kg i.p.) for 7 days significantly ameliorated the levels of AST, ALT and ALP elevated by the CC14 treatment alone. The results of biochemical tests were also confirmed by histopathological examination. The livers of the group treated with VL showed less balooning degeneration and apoptosis. Necrosis had not been observed in VL group. These results suggest that, liver damage occured in VL-treated group is less than the damage occured in silibinin-treated group. To evaluate of hypoglicemic activity of VL, glibenclamide was used as the reference agent. But, VL has not hypoglycemic activity in healthy and diabetic mice.
Hepatoprotective and Tnf-Α Inhibitory Activity of Zosima Absinthifolia Extracts and Coumarins
(Elsevier, 2011) Bahadir, Ozlem; Citoglu, Gulcin Saltan; Ozbek, Hanefi; Dall'Acqua, Stefano; Hosek, Jan; Smejkal, Karel
Zosima absinthifolia (ZA) extracts and the coumarins (+)-columbianadin and (-)-deltoin were evaluated for their potential hepatoprotective and antiinflamatory effects in a CCl4-induced hepatotoxicity assay in rats and by the inhibition of TNF-alpha production on LPS-stimulated THP-1 macrophages, respectively. Both the ZA extracts and the coumarins showed hepatoprotective activity confirmed by monitoring the ALT/AST levels and by histopathological examination. The antiinflamatory activity, proved by the inhibition of TNF-alpha production, was found to be higher for the n-hexane root extract than for coumarins, suggesting synergic potential of the extract. The concentration of (-)-deltoin and (+)-columbianadin in extracts was determined by HPLC analysis. (c) 2011 Elsevier B.V. All rights reserved.
The Hepatoprotective Effects of Dihydromyrcenol and Geranyl Formate in an Experimental Model of Acute Hepatic Injury Induced by the Use of Carbon Tetrachloride
(Aves, 2011) Bayram, Irfan; Erten, Remzi; Bayram, Yasemin; Bulut, Gulay; Ozbek, Hanefi
Background/aims: We aimed to investigate the hepatoprotective effects of dihydromyrcenol and geranyl formate extracted from the Vitis vinifera L. plant in a rat model of acute hepatic injury induced by carbon tetrachloride. Methods: The study was performed on 54 Sprague-Dawley male rats. The animals were divided into 9 study groups with 6 rats in each. At the end of the 7-day study period, the animals were sacrificed. The effects of dihydromyrcenol and geranyl formate on hepatic injury were evaluated based on the comparisons of the changes in the weight, serum levels of alanine aminotransferase and aspartate aminotransferase, and histopathological changes in the liver. Results: Dihydromyrcenol significantly reduced the carbon tetrachloride-associated ballooning degeneration and apoptotic cell counts; this reduction was moderate with low doses of geranyl formate, while no reductions were observed with high doses of geranyl formate. The changes in the alanine aminotransferase and aspartate aminotransferase levels were in accordance with these findings. Conclusions: It can be concluded that in an experimental model of acute hepatic injury induced by carbon tetrachloride, dihydromyrcenol presents a hepatoprotective effect, while geranyl formate presents partial hepatoprotective effects at low doses and no hepatoprotective effects at high doses.
High-Cholesterol Diet Increases Xanthine Oxidase and Decreases Nitric Oxide Synthase Activities in Erythrocytes From Rats
(Pergamon-elsevier Science Ltd, 2008) Devrim, Erdinc; Erguder, Imge B.; Ozbek, Hanefi; Durak, Iiker
The aim of this study was to investigate the effects of a high-cholesterol diet on oxidant/antioxidant status and nitric oxide synthase (NOS) activity in erythrocytes from rats. Sixteen Sprague-Dawley-type albino male rats were used in the study. The rats were randomly divided into 2 groups: the control group (group 1) was fed a standard rat diet, and the treated group (group 2) was fed a high-cholesterol diet (4% cholesterol, 1% cholic acid, and 0.5% thiouracil) in addition to standard pellet rat diet for 3 months. At the end of the study period, blood samples were obtained from the rats under ether anesthesia. Oxidant (malondialdehyde level, sensitivity to oxidation value, and xanthine oxidase [XO] activity) and antioxidant parameters (antioxidant potential value, superoxide dismutase, catalase, and glutathione peroxidase activities) were studied in erythrocyte preparations. Activities of erythrocyte NOS and arginase enzymes and serum total cholesterol levels were also measured. We observed that serum total cholesterol levels, erythrocyte XO activities, and sensitivity to oxidation values significantly increased in group 2 (cholesterol fed) compared with the control group (group 1). Erythrocyte NOS activities were also found to decrease in group 2. In conclusion, our results suggest that cholesterol feeding causes an increase in XO activity and a decrease in NOS activity in the erythrocytes from rats. The increase in XO activity may render the erythrocyte membranes sensitive to oxidant stress, and the decrease in NOS activity in the erythrocytes may increase cardiovascular disease risk via reduced endothelial relaxation. (c) 2008 Elsevier Inc. All rights reserved.
Investigation of the Hepatoprotective Effects of Sesame (Sesamum Indicum L.) in Carbon Tetrachloride-Induced Liver Toxicity
(Springer, 2013) Cengiz, Nureddin; Kavak, Servet; Guzel, Ali; Ozbek, Hanefi; Bektas, Hava; Him, Aydin; Balahoroglu, Ragib
More than 600 chemicals can cause damage in liver, one of which is carbon tetrachloride (CCl4). Hepatoprotective agents could prevent tissue damage and reduce morbidity and mortality rates; such agents may include alternative or folkloric treatments. We investigated sesame (Sesamum indicum L.) for its hepatoprotective effect in CCl4-induced experimental liver damage. To this end, 0.8 mg/kg of sesame fixed oil was provided intraperitoneally to rats whose livers were damaged by CCl4. Tissue and blood samples were taken at the end of the experiments and evaluated histologically and biochemically. Ballooning degenerations and an increase in lipid droplets in liver parenchyma and increases in serum alanine transaminase, aspartate transaminase, and bilirubin were found in the CCl4 group. Biochemical and histopathological findings in the sesame fixed oil treated group were not significantly different from the CCl4 group. Sesame did not show a hepatoprotective effect in CCl4-induced liver toxicity.
Investigation on Median Lethal Dose (Ld50) and Hypoglycemic Effect of Thymus Fallax F. Leaves Essential Oil
(Studium Press Llc, 2009) Ozbek, Hanefi; Ozturk, Mustafa; Ozgoce, Fevzi
We aimed to investigate median lethal dose (LD50) and hypoglycemic effect of Thymus fallax F. leaves essential oil (TF) on healthy and diabetic mice. TF, glibenclamide (as a reference group) and isotonic saline solution (ISS) (control group) were administrated to the healthy and alloxan-induced diabetic mice. Blood samples were drawn from the vena coccygea of mice before treatment and in the 1st, 2nd, 3rd, 4th and 24th hour for measurement of glucose levels. When compared with ISS group TF decreased blood glucose values significantly in diabetic mice in the second hour. Glibenclamide decreased blood glucose values significantly in the 1st, 2nd, 4th and 24th hour. There was no significant difference of blood glucose values among groups of healthy mice. The median lethal dose (LD50) of the TF was determined at 0.741 mL/kg.
The Levels of Nitrite, Nitrate and Lipid Peroxidation in Diabetic Mouse Brain: the Effect of Melatonin and Pentoxifylline
(Taylor & Francis Ltd, 2022) Yalcinkaya, Ahmet S.; Sekeroglu, Mehmet Ramazan; Huyut, Zubeyir; Cokluk, Erdem; Ozbek, Hanefi; Ozturk, Gurkan; Balahoroglu, Ragip
Objective: This study investigated the relationship between diabetes (DM) and nitrite, nitrate and MDA levels and effect of melatonin and pentoxifylline. Methods: Sixty mice were randomly divided into four groups. Control: no action; Diabetes group (DM): after fasting-blood-glucose (FBG) was measured, 150 mg/kg alloxane was applied intraperitoneally three-times every other day; Diabetes + Melatonin (DM + MLT) and Diabetes + Pentoxifylline groups (DM + PTX): following the same procedures with DM, 10 mg/kg melatonin and 50 mg/kg pentoxifylline were administered subcutaneously six days, respectively. Following FBG analysis, brain tissues were taken under the anaesthesia. Nitrite, nitrate and MDA levels were measured. Results: In the all groups with alloxane, FBG were higher than in before application (p < .05). Also, FBG, nitrite, nitrate and MDA levels in the DM + MLT and DM + PTX groups were lower than in the DM (p < .05). Conclusions: Nitrite and nitrate may be related to etiopathogenesis of DM, and pentoxifylline and especially melatonin relatively decrease nitrite, nitrate and lipid peroxidation.
The Susceptibility To Autoxidation of Erythrocytes in Diabetic Mice: Effects of Melatonin and Pentoxifylline
(Wiley, 2017) Sekeroglu, Mehmet Ramazan; Huyut, Zubeyir; Cokluk, Erdem; Ozbek, Hanefi; Alp, Hamit Hakan
Oxidative stress had a great importance in development of complications in diabetes. We investigated effects of melatonin and pentoxifylline in diabetic mice. Swiss albino mice (n=40) were divided into four groups: alloxan-induced diabetes mellitus (DM), alloxan-induced diabetes with melatonin supplementation (DM+MLT), alloxan-induced diabetes with pentoxifylline supplementation (DM+PTX), and control. Glutathione-peroxidase (GSH-Px) activity, malondialdehyde (MDA) and reduced glutathione (GSH) levels, and susceptibility to oxidation of erythrocytes were measured. MDA levels were higher than control in the DM and DM+MLT. The DM had more MDA level than the DM+MLT and DM+PTX (P<0.001). After in vitro oxidation, MDA levels of all groups were found higher than the control. However, they were significantly lower than the DM in DM+PTX and DM+MLT (P<0.001). Although GSH levels of the DM and DM+PTX were less than the control, GSH-Px activity of the DM was lower than the control and DM+PTX (P<0.05). We suggest that there is increased oxidative stress and compromised antioxidant status of erythrocytes in diabetes; however, it can be effectively prevented by melatonin or pentoxifylline supplementation.