Browsing by Author "Ozdemir, Fethi Ahmet"

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Comparison of Germination Abilities of Centaurea Sp Under in Vivo and in Vitro Conditions
(Mattioli 1885, 2017) Demirel, Sevda; Turkoglu, Nalan; Ozdemir, Fethi Ahmet
In this study, in vivo and in vitro germination of endemic and under-threat Centaurea fenzlii and Centaurea derderifolia species were investigated. In in vivo germination, seeds were spread with forceps over the drying papers moisturized with distilled water in petri dishes. Then, petri dishes were closed and taken for germination in a climate chamber at 25 degrees C and 16 hours light/8 hours dark photoperiods. In in vitro germination, following the sterilization period, seeds were cultured in MS, B5, SH and WH growth medium with 6 seeds in each in a climate chamber at 25 degrees C under 16 hours light/8 hours dark photoperiod. Present findings revealed high germination rates for C. fenzlii and C. derderifolia seeds in WH growth medium.
The Effects of Phosphorus and Humic Acid on Some Soil Properties Flowers Quality of Gladiolus
(Mattioli 1885, 2017) Cavusoglu, Mehmet Nuri; Turkoglu, Nalan; Ozdemir, Fethi Ahmet
This study was conducted to determine the effects of various phosphorus and humic acid applications on the development of gladiolus varieties in pot culture. This study was carried out between May 2014 and April 2015. Rose Supreme, Red, and Essential cultivars were grown in soiled pods with 0, 100, 200 mg/kg phosphorus and 0, 1000, 2000 mg/kg humic acid doses. We found that application of humic acid and phosphorus had positively contributed to N and P contents in the soil as well as floret number of gladiolus and flower-stalk length. As a result of the study, optimum doses were determined as 2000 mg/kg humic acid and 200 mg/kg phosphorus with regard to the studied characteristics.
The Effects of Uv Irradiation and Incubation Time on in Vitro Phenolic Compound Production in 'karaerik Grape Cultivar
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2020) Celik, Mustafa; Keskin, Nurhan; Ozdemir, Fethi Ahmet
In this study, the effect of Ultraviolet (UV) irradiation on induction of individual and total phenolics production on callus cultures of 'Karaerik' grape cultivar was investigated. Callus tissues were obtained from the leaves of the cuttings grown in in vitro plants. As a culture medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA (Naphthaleneacetic acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissues were sub-cultured twice with 21 days intervals. After the second subculture, 12-day-old callus tissues were exposed to 254 nm UV-C light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. After the treatment, callus tissues were incubated under dark conditions. Phenolic compounds were measured at 24th, 48th and 72nd hours. Individual phenolic compounds were analyzed by HPLC (High Pressure Liquid Chromatography) and total phenolic compounds were measured by spectrophotometer. As a result of the study, it was found that UV irradiation was effective for induction the production of phenolic compounds in the callus tissues of 'Karaerik' grape cultivar and this effect was closely related to the application time.
Efficient Protocol for Multiple Micro-Shoot Regeneration From Endanger, Endemic and Ornamental Plant Centaurea Fenzlh Reichardt
(Parlar Scientific Publications (p S P), 2018) Turkoglu, Nalan; Ozdemir, Fethi Ahmet; Keskin, Nurhan; Khawar, Khalid Mahmood
Environment damage has brought about the elimination of many plant species, and numerous more are endangered. Similarly, ornamental and endemic Centaurea fenzlii Reichardt that grows in Turkey is threatened that necessities measures for its immediate conservation. In vitro culture techniques are widely used to conserve uncommon, threatened plants. This study reports a proficient technique for multiple micro-shoot regeneration of C. fenzlii. A low recurrence (35%) germination was acquired on MS medium supplemented with 2.60 mu M GA(3). Juvenile leaves were utilized as an explants source. The leaves were regenerated on MS medium that contained 1.11, 2.22, 4.44, 8.88 mu M BAP + 2.685, 5.37, 10.64 mu M NAA. The best callus regeneration percentage was seen on MS medium that used 4.44 mu M BAP + 10.64 mu M NAA. Callus regeneration percentage extended between 21.33 to 66.87. The most noteworthy micro-shoot regeneration percentage was gotten on MS medium containing 8.88 mu M BAP + 2.685 mu M NAA. Micro-shoot regeneration percentage run between 5.66 to 36.87%. The highest number of micro-shoots per explants was noted on MS medium containing 4.44 mu M BAP + 10.64 mu M NAA. Also number of micro-shoots per explants extended between 1.66 to 5.87. The rooting percentage of C. fenzlii was low with a few roots. The best rooting was noted following seven weeks of culture on 4.90 M IBA. IBA appears to act as stimulant in rooting of C. fenzlii.
In Vitro Propagation and Secondary Metabolites Investigation of Hypericum Perforatum L
(Parlar Scientific Publications (p S P), 2019) Mohammed, Namam Rebaz; Dalar, Abdullah; Ozdemir, Fethi Ahmet; Turker, Musa
Hypericum perforatum L. was regenerated in plant tissue culture and secondary metabolites (hypericin, pseudohypericin, quercetin, rutin, and chlorogenic acid) of the plants collected from field and regenerated in vitro were quantitatively compared. The liquid, semi solid and solid form of Murashige and Skoog (MS) basal medium supplemented with plant growth regulators (PGRs) in different concentration and combination were employed for regeneration and secondary metabolite product amplification. Based on preliminary tests nodal segment was preferred as an explant. At the end of 50 days of regeneration period, no statistically significant difference was observed between the length of root and shoot and root number of plants regenerated in solid, semi solid and liquid media supplemented with different PGRs. The quantitative secondary metabolite analyses was performed by High Performance Liquid Chromatography (HPLC). The highest concentrations of chlorogenic acid, quercetin, and pseudohypericin were observed in shoots and roots of the plants collected from field. Whereas the compounds were detected in low quantity in plants regenerated in the liquid, semi solid and solid medium, except for quercetin which was found higher concentration than chlorogenic acid, quercetin, and pseudohypericin in vitro regenerated plants. Hypericin and rutin were not detected by HPLC analysis in all of plants regenerated in vitro and in vivo.
Phenolic Compound Production Increased in Vitro Regenerated Cichorium Intybus L
(Mdpi, 2023) Abas, Yousif Abdullah; Eroglu, Ayten; Dalar, Abdullah; Turker, Musa; Ozdemir, Fethi Ahmet; Solowski, Gawel
Chicory (Cichorium intybus L.) is a low-height perennial or biennial herb from the family of Asteraceae. Investigation of different in vitro regeneration strategies of Cichorium intybus and increasing the number of secondary metabolites in vitro regenerated plant samples were the aims of the research. Callus and plant regenerations were achieved in basal plant growth media supplemented with plant growth regulators (PGRs). Whole plant regeneration was carried out by direct organogenesis from leaf explant in Murashige and Skoog (MS) and B5 media supplemented with naphthalene acetic (NAA) acid and indole-3-butyric acid (IBA). The highest callus quantity was produced in MS medium supplemented with indole-3-acetic acid (IAA) and benzyl amino purine (BAP). The combination and concentrations of PGRs used in MS and B5 media not only provided root and shoot formation with callus, but also caused a change in the amounts of phenolic components. In addition, some PGRs used caused an increase in the number of phenolic compounds in callus and shoots developed from the leaf explant. When plants that grow in vitro and in vivo are compared with each other, it has been determined that plants grown in vivo contain higher amounts of some phenolic compounds. In vivo and in vitro samples were extracted in ethanol/water (80:20 v/v). The analysis of phenolic compounds (caftaric, chicoric, and chlorogenic acids and esculin) were performed in high-performance liquid chromatography (HPLC) and inulin was in UV spectrophotometry. The caftaric and chlorogenic acids and inulin concentrations were higher in vivo samples than that in vitro. Contrarily, esculin, and chicoric acid concentrations were higher in the in vitro regenerated samples. The higher concentration of valuable compounds in the in vitro regenerated samples, especially in callus tissue, gives hope for large-scale production of secondary metabolites under laboratory conditions.
Rapd Markers Reveal Genetic Variation Between Cichorium Spinosum L. and Taraxacum Sp.; a Substantial Medicinal Plants of Greece
(Mattioli 1885, 2018) Shidfar, Mina; Keskin, Siddik; Khah, Ebrahim M.; Spiridon, Petropoulos; Ozdemir, Fethi Ahmet; Gokcen, Ibrahim Samet
Fifteen Random Amplified Polymorphic DNA (RAPD) markers were used to measure genetic diversity and genetic relationships between five endemic genera of Mediterranean basin (Greece). Three species of Cichorium spinosum were collected; two from island Crete (Greece) and one from island Kythnos, and other two species of Taraxacom sp. (Asteraceae); are from Orhomenos and Athens. Two hundred-forty amplified products and 163 RAPD bands were scored with an average of 67.91% of them revealing polymorphism across accessions. In this research OPD-05 primer with 24 bands showed the highest number of bands, while the OPM-18 and OPB-16, both of them with 8 bands showed the least number of bands. Also OPV-06 primer with 18 polimorphic bands showed the highest number of bands. The least number of polymorphic bandswere found in OPX-18. UBC-292, OPAN-01, OPB-16, OPM-18, OPD-05 primers. Subsequently, OPD-05 primer with 29.16% showed the least percentage of polymorphism degree, whereas OPM-18 and OPB-16 primers with 87.50% showed the highest percentage of polymorphism. UPGMA clustering based on data from polymorphic RAPD bands revealed two distinct group which joined to form one major cluster at 32% level of similarity. Also Cichorium spinosum, Crete and Cichorium spinosum, Kythnos, varieties with 100% similarity are synonyms. The similarity indices of the RAPD dendrogram ranged between 30% and 100% averagely high enough to suggest useful variability for genetic diversity and plant breeding.
Β-Carboline Production From in Vitro Regenerated Peganum Harmala L. Plant
(Parlar Scientific Publications (p S P), 2018) Onlu, Seyma; Bingol, Omer; Turker, Musa; Ozdemir, Fethi Ahmet
Peganum harmala L. seeds were germinated in hormone free sterile MS medium. Root, shoot tip, leaf, petiole, hypocotyl, cotyledon and nod explants excised from in vitro regenerated plants were incubated in MS medium supplemented with 1 mg/l and 2 mg/l 2,4-D for callus production. Hypocotyl and cotyledon explants produced callus and untreated callus was accepted as control for the comparison of beta-carboline alkaloids production extracted from different samples. Callus were exposed to cold and dark conditions and incubated in MS medium supplemented with 2 mg/l ABA, 1 mg/l ABA, 1 mg/l NAA and 1 mg/l BAP in different batches to increase beta-carboline alkaloid production. Seeds were germinated in soil ambient condition. beta-carboline alkaloids; harmalol, harmine and harmaline were extracted from seeds, plants grown in soil, callus grown in MS medium supplemented with 2,4-D, callus incubated MS medium supplemented with NAA, BAP, ABA and callus exposed cold and dark condition. The extracts were quantitatively analysed with HPLC. The highest alkaloid concentrations were determined in vivo seeds and plants grown in soil. However, alkaloid production in callus tissues is also promising. Harmalol and harmine concentration decreased with cold treatment but increased with ABA treatment compared to that of the control. Harmaline was increased with cold treatment, but dramatically decreased in all treatment that callus is exposed.