Browsing by Author "Ozel, Seda"

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Oxidative Stress and Altered Levels of Oxidants and Antioxidants in Acute Ischemic Stroke Patients in a Region of East Turkey
(Drunpp-sarajevo, 2012) Ozbey, Ulku; Seyran, Ayse; Erisir, Mine; Ozel, Seda
Background and purpose: Ischemic stroke is one of the most important diseases causing severe disability and death. Hypertension is the most important risk factor in strokes, representing roughly 70% of all cases. Oxidative stress plays an important role in acute ischemic stroke (AIS) pathogenesis. It is believed to be one of the mechanisms taking part in neuronal damage in stroke. Free radical formation and subsequent oxidative damage may be a factor in stroke severity. In 28 patients with AIS and 28 healthy individuals were investigated with regard to relationship between ischemic-stroke and glutathione peroxidase (GSH-Px), catalase (CAT) activities, malondialdehyde (MDA), glutathione, beta-carotene, vitamin A levels. Methods: GSH-Px, CAT activities and MDA, GSH, vitamin A, beta-carotene levels were measured by spectrophotometry in 28 subject (15 females and 13 males aged 67.1 +/- 13.3years) with AIS and 28 age-matched healthy subjects (13 females and 15 males aged 65.9 +/- 10.1). Results: In the patients with acute ischemic stroke, GSH-Px activity and MDA, GSH, vitamin A levels were found increased (p<0.001). Whereas there was no significant difference in level of beta-carotene and CAT activity between AIS patients and control group (p>0.005). Conclusions: Our results suggest deleterious effects of oxidative stress on clinical outcome in AIS. There was a significant increase in levels of MDA, GSH-Px and GSH. GSH elevation seemed to be a part of adaptive mechanisms
The Relationship Between Blood Antioxidant Enzyme Levels and Genotype During Migraine Attack and Initial Periods in Migraine Patients
(Walter de Gruyter Gmbh, 2011) Ozbey, Ulku; Ozel, Seda; Seyran, Ayse; Erisir, Mine; Berilgen, M. Said
Objective: In recent years, researchers have been focused on the importance of oxidative stress on migraine pathogenesis. We aimed to investigate the roles of Superoxide Dismutase (SOD), Glutathione Peroxidase (GSH-Px), Catalase (CAT), antioxidant Glutathione (GSH) and Malondialdehyde (MDA) of a lipid peroxidation product in migraine pathogenesis. Furthermore, we also aimed to investigate the incidence of the gene polymorphisms of these enzymes in Turkish population. Material and Methods: GSH-Px, CAT, SOD activities and plasma MDA and blood GSH levels were measured in 40 migraine patients at attack and initial period and 40 healthy subjects. The genotype characteristics were determined by PCR-based RFLP method using DNA extracted from peripheral blood. Results: Statistically significant decrease in GSH-Px, CAT, SOD activities and GSH levels were found in the migraine patients compared to the control group (GSH, CAT p < 0.001; GSH-Px, SOD p < 0.05). In the migraine patients and control groups no significant differences were observed for Mn-SOD and CAT genotypes, but there was a significant difference in the GSH-Px3 genotype. No difference was detected in the allele frequencies of MnSOD, GSH-Px3 and CAT gene between the patients and control group. Conclusion: Consequently, oxidative stress and antioxidant enzymes may play an important role in migraine pathogenesis, and according to our data GSH-Px3 gene polymorphism may cause a tendency to migraine for our regional community. Conflict of Interest: There is no conflict of interest between autors.