Browsing by Author "Ozercan, Ibrahim Hanifi"

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A Comprehensive Immunohistochemical Examination of the Distribution of the Fat-Burning Protein Irisin in Biological Tissues
(Elsevier Science inc, 2014) Aydin, Suleyman; Kuloglu, Tuncay; Aydin, Suna; Kalayci, Mehmet; Yilmaz, Musa; Cakmak, Tolga; Ozercan, Ibrahim Hanifi
Irisin was first identified in skeletal muscle cells, but its precise location has not yet been demonstrated, and there is limited information about irisin protein in other human and rat tissues. The present immunohistochemical study was undertaken to screen skeletal muscle and other tissues for irisin immunoreactivity.. Irisin staining was found in the brain (neurons and neuroglia), cardiac and skeletal muscle (fibers) and skin (sebaceous glands) tissues in male rats. In both human adult and fetal skeletal muscle, the most intense immunohistochemical staining was in the perimysium and endomysium, in the peripheral nerve (epineurium) and axon and nerve sheaths spreading among the cells, in the sarcoplasma and subendomysium. Irisin was also demonstrated in the testis (seminiferous tubules, some spermatogenic cells in fetal and Leydig cells in fetal and adult testis, ductus epididymis in fetal human epididymis); pancreas (islets of Langerhans, serous acini cells, intralobular and intralobular ducts cells); liver (hepatocytes; Kupffer cells and sinusoidal endothelial cells); spleen (subcapsular region and periarterial lymphatic sheets); the stomach (gastric parietal cells, tunica muscularis cells). We conclude that the fat-burning protein irisin locally produced in peripheral and central tissues could act as a gatekeeper of metabolic energy regulation in those tissues, since this myokine converts white into brown adipose tissue, enhancing energy expenditure. (C) 2014 Elsevier Inc. All rights reserved.
Protective Effects of L-Carnitine, N-Acetylcysteine and Genistein in an Experimental Model of Liver Fibrosis
(Elsevier Masson, Corporation office, 2014) Demiroren, Kaan; Dogan, Yasar; Kocamaz, Halil; Ozercan, Ibrahim Hanifi; Ilhan, Selcuk; Ustundag, Bilal; Bahcecioglu, Ibrahim Halil
Aim: Liver fibrosis is a reversible wound-healing response that occurs following liver injury. In this study, we aimed to investigate the possible protective effects of L-carnitine, N-acetylcysteine and genistein in liver fibrosis induced by carbon tetrachloride (CCl4). In addition, the effects of these agents were compared in the same study. Methods: In this study, rats were randomly allocated into 8 groups, consisting of 10 rats each, as follows: a control group, CCl4, L-carnitine, N-acetylcysteine, genistein, CCl4 and L-carnitine, CCl4 and N-acetylcysteine, and CCl4 and genistein. At the end of 6 weeks, blood and liver tissue specimens were collected. Alanine aminotransferase (ALT); aspartate aminotransferase (AST); complete blood count, tumor necrosis factor-alpha (TNF-alpha); platelet-derived growth factorBB (PDGF-BB); interleukin-6 (IL-6); liver glutathione level; oxidant/antioxidant status; scores of hepatic steatosis, necrosis, inflammation, and fibrosis; and the expression of alpha-smooth muscle actin were studied. Results: Although the ALT and AST values in the group administered CCl4 were significantly higher than in all the other groups (P < 0.05), there was no significant difference between the control group and the groups administered CCl4 combined with L-carnitine, N-acetylcysteine and genistein (P > 0.05). There were significant differences in the levels of TNF-alpha, PDGF-BB and IL-6 (P < 0.05) between the CCl4 group and the groups with L-carnitine, N-acetylcysteine and genistein added to CCl4. N-acetylcysteine and genistein had positive effects on the oxidant/ antioxidant status and on liver necrosis and fibrosis scores. Conclusions: In our study, L-carnitine, N-acetylcysteine and genistein showed significant protective effects in liver fibrosis induced by CCl4. (C) 2013 Elsevier Masson SAS. All rights reserved.