Browsing by Author "Ozhan, Gunes"

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Lc-esi-ms/Ms Analysis of Secondary Metabolites of Different St. John's Wort (Hypericum Perforatum) Extracts Used as Food Supplements and Evaluation of Developmental Toxicity on Zebrafish (Danio Rerio) Embryos and Larvae
(Elsevier, 2023) Atalara, Mehmet Nuri; Kokturk, Mine; Altindag, Fikret; Ozhan, Gunes; Ozen, Tevfik; Demirtas, Ibrahim; Gulcini, Llhami
Hypericum perforatum (St. John's wort) belongs to the Hypericaceae family and is one of the best known Hypericum species worldwide. It is a very popular and valuable medicinal plant widely distributed in Anato-lia. Hypericum perforatum contains many bioactive components that play a role in activities has been used as a food supplement. The extracts are used within safe dose range that are harmless and effective for health. When the SJW1, SJW2 and SJW3 fractions of St. John's Wort extracts were exposed to zebrafish embryos and larvae at different concentrations (5, 10, 100, and 300 mg/mL), the survival rates at 96th hour were deter-mined as 83.3, 27.5 and 2.5%, respectively. No significant changes were found in the malformation rates, and the larval emergence was found to be above 80% at 96th hour for all extracts. No caspase-3 expression was found at the 96th hour in the larvae. Similar secondary components of extracts were observed except quanti-tative differences. The use of samples in doses of 10 mg/mL and below as food supplement may be harmless, however, threshold dose values of H. perforatum extracts lower toxic doses may be due to the different amounts of secondary metabolites.& COPY; 2023 Published by Elsevier B.V. on behalf of SAAB.
Textile Dyes Maxilon Blue 5g and Reactive Blue 203 Induce Acute Toxicity and Dna Damage During Embryonic Development of Danio Rerio
(Elsevier Science inc, 2021) Kokturk, Mine; Altindag, Fikret; Ozhan, Gunes; Calimli, Mehmet Harbi; Nas, Mehmet Salih
Common textile dyes used in various industrial sectors are organic compounds and considered for the aquatic environment as pollutants. The textile dye industry is one of the main sectors that have serious impacts on the environment due to a large amount of wastewater released into the ecosystem. Maxilon blue 5G (MB-5G) and Reactive Blue 203 (RB-203) are widely used textile dyes. However, their potential toxicity on living organisms remains to be elucidated. Here, we investigate the acute toxicity and genotoxicity of MB-5G and RB-203 dyes using the zebrafish embryos/larvae. Embryos treated with each dye for 96 h revealed LC50 values of acute toxicity as 166.04 mg L-1 and 278.32 mg L-1 for MB-5G and RB 203, respectively. When exposed to MB-5G and RB-203 at different concentrations (1, 10, and 100 mg L-1) for 96 h, the expression of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, significantly increased in brain tissues as compared to control. MB-5G and RB-203 resulted in common developmental abnormalities including tail malformation, microphthalmia, pericardial edema, curved body axis, and yolk sac/pericardial edemas. Moreover, at its highest dose (100 mg L-1), RB-203 caused premature hatching after 48 h, while MG-5G did not. Our results collectively reveal that the textile dyes MB-5G and RB-203 cause genotoxicity and teratogenicity during embryonic and larval development of zebrafish. Thus, it is necessary to eliminate these compounds from wastewater or reduce their concentrations to safe levels before discharging the textile industry wastewater into the environment.
What Is the Eco-Toxicological Level and Effects of Graphene Oxide-Boramidic Acid (go-Ed Np) ?: in Vivo Study on Zebrafish Embryo/Larvae
(Elsevier Sci Ltd, 2022) Kokturk, Mine; Yildirim, Serkan; Yigit, Aybek; Ozhan, Gunes; Bolat, Ismail; Alma, Mehmet Hakki; Atamanalp, Muhammed
Graphene oxide (GO) and their natural/synthetic composites are encouraging tools for humanity. There is a need to address critical challenges and potential risk possibilities in GO-based architectures, which have a wide range of uses. In this study, ecotoxicological levels as well as GO-based nanoparticle synthesis, characterization, interaction mechanism and toxicity detection levels for potential biomedical applications were determined on zebrafish (Danio rerio). The effects of GO-ED-BA NP (graphene oxide-boramidic acid nano particles) which was characterized by FT-IR, SEM, TEM, and BET on survival rate, morphological abnormalities (yolk sac edema, lordosis/kyphosis, pericardial edema, and tail malformation), hatching rate as well as neuronal degeneration /necrosis, 8 OHdG and TNF-alpha expression were observed in D. rerio embryos and larvae. In the obtained findings, it was determined that the toxicity profile of GO-ED-BA NP appeared similar, in high-dose application with single GO use, causing a cytotoxic, pro-inflammatory response and triggering oxidative stress. However, increased malformation rates and mortality at the highest concentration were due to nanoparticle sizes and GO. The presence of boramidic acid unit on graphene nanostructure changed the GO's toxicity profile and positively directed the proinflammatory and oxidative stress response. Synthesizing of graphene oxide-boramidic acid and its toxicity panels compared to graphene oxide are reported for the first time in this study.