Browsing by Author "Ozkan, Cumali"

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Atropine Sulphate Test Can Be an Aetiologic Indicator of Vagal Bradycardia Developed in a Megaoesophagus Case
(Natl veterinary Research inst, 2007) Keles, Ihsan; Altug, Nuri; Kaya, Abdullah; Ozkan, Cumali; Yuksek, Nazmi; Aslan, Logman
An acquired megaoesophagus in a calf has been described. The diagnosis of megaoesophagus was made based on clinical and laboratory examinations, using contrast radiography, and necropsy findings. Atropine sulphate was used to enlighten the relationship between damage in the N. vagus and bradycardia, which developed in the megaoesophagus case.
Clinical Coenurosis (Coenurus Cerebralis) and Associated Pathological Findings in a Calf
(Univ Agriculture, Fac veterinary Science, 2011) Ozkan, Cumali; Yildirim, Serkan; Kaya, Abdullah
This study aims to investigate clinical and pathological findings of a clinical Coenurus cerebralis case in a 10-month-old Simmental male calf. Clinical examination of the calf revealed incoordination, irregular gait, failure to hold the head straight, leftward head tilt, and circling. The animal was diagnosed with C. cerebralis and euthanazia was recommended. The autopsy demonstrated a cyst (9x7 cm) in the caudal of the left cerebral hemisphere within the cranium. The cyst caused compression over the ventral portion of the left cerebral hemisphere, while a marked perforation of 3-4 cm diameter was found on the sphenoid bone. Histopathologically, hyperemia and perivascular mononuclear cell infiltration were observed. In conclusion, we found it beneficial to present the clinical and pathological findings of this calf infected with C. cerebralis which is known to be a rare clinical entity among cattle. (C) 2011 PVJ. All rights reserved
Detection of Se, Vit. E, Vit. A, Mda, 8-Ohdg, and Coq10 Levels and Histopathological Changes in Heart Tissue in Sheep With White Muscle Disease
(Humana Press inc, 2019) Yildirim, Serkan; Ozkan, Cumali; Huyut, Zubeyir; Cinar, Ali
This study was carried out to determine vit. E, Se, vit. A, malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and ubiquinone-10 (CoQ10) levels and histopathological changes in sheep with white muscle disease (WMD). A total of 30 sheep were used; 20 sheep with WMD were brought to our clinic for diagnosis and treatment at various times, and 10 healthy sheep were in the control group. The Se, vit. E, vit. A, MDA, 8-OHdG, and CoQ10 values of the healthy and WMD sheep were as follows: 0.917 +/- 0.037, 0.790 +/- 0.067; 1.190 +/- 0.011, 1.090 +/- 0.021; 5.400 +/- 0.275, 5.200 +/- 0.173; 1.602 +/- 0.264, 2.636 +/- 0.576; 0.656 +/- 0.197, 1.485 +/- 0.271; and 0.280 +/- 0.044, 1.753 +/- 0.551 respectively (p<0.05). According to histopathological and immunohistochemical findings in the WMD group, hyaline degeneration, Zenker's necrosis, and dystrophic calcification were observed in the muscle fibers. Immunohistochemically, 8-OHdG staining of the heart tissue determined a severe 8-OHdG expression in the WMD group. The findings of this study suggest that MDA, 8-OHdG, and CoQ10 values could be used as diagnostic and prognostic biomarkers in sheep affected with WMD.
Determination of Cryptosporidium Spp. in Van Cats by Nested Pcr
(Polish Soc veterinary Sciences Editorial office, 2018) Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Goz, Yasar; Ozkan, Cumali; Denizhan, Vural
The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YuzuncuYil University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens - a total of 100 Van cats were analyzed in the study. A simplified formol-ether concentration method was applied to all samples. The samples were then examined microscopically by a modified Ziehl-Neelsen staining method. As a result of the staining, Cryptosporidium spp. oocysts were identified in stool samples of 3 kittens in the microscopic examination. After that, PCR and nested-PCR were conducted with suitable primers. Nested PCR identified 5 kittens (5%) as positive. As a result, it was concluded that nested PCR was a superior diagnostic method for Cryptosporidium diagnosis compared with staining methods and that infected cats could be a health hazard for other cats and individuals, since Cryptosporidium spp. agents infect via the faecal-oral route. Therefore, we believe it is necessary to raise the awareness of people in contact with cats.
Determination of Selected Hormones and Biochemical Parameters in Turkish Van Cats With Tail Gland Hyperplasia
(Polish Soc veterinary Sciences Editorial office, 2020) Ozkan, Cumali; Kayar, Abdullah; Kaya, Abdullah; Yildirim, Serkan; Iskefli, Onur; Kozat, Suleyman; Akgul, Yakup
The present study was performed on Turkish Van cats with tail gland hyperplasia in order to determine changes in some hormone levels and biochemical parameters. Animal material consisted of 18 male Van cats of between 2-8 years of age, 12 with tail gland hyperplasia and 6 unaffected cats as the control group. Following routine clinical examination, hormone levels and biochemical parameters were measured by obtaining blood samples, and biopsy samples were obtained from the region and examined under a light microscope. When compared with the control group, testosterone levels were high although the difference between groups was not statistically significant. Similarly, no statistical significance was found between the groups in terms of other hormones. Among the hormonal parameters, the only remarkable increase was seen in the testosterone level. Among biochemical parameters, the alkaline phosphatase (ALP), aspartate amino transferase (AST), cholesterol and triglyceride levels were statistically different, while other biochemical parameters were not statistically significant. While AST and cholesterol results were increased, ALP and triglyceride levels were decreased. As a result, we conclude that the disease is an important problem which occurs extensively in mature male Van cats, particularly during and after mating periods. We also conclude that the disease might be associated with an increase in testosterone levels. Presenting this study is beneficial as there are limited reports about this disease in cats. This is the first study presenting tail gland hyperplasia with some hormonal levels and biochemical parameters in male Van cats.
Diagnosis and Treatment of Hypophosphatemia in Young Turkeys
(Univ Agriculture, Fac veterinary Science, 2012) Ozkan, Cumali; Kaya, Abdullah; Aslan, Logman; Akgul, Yakup
This study was aimed to diagnose and to treat the disease of young turkeys showing hypophosphatemia symptoms in a farm. The material of this study was consisted of turkeys in both sexes, aged between 13-15 weeks. In the clinical examinations of the turkeys; weight loss, weakness, unwilling to walk, lameness and extremity fractures in some animals were determined. Blood samples were collected from 20 turkeys chosen randomly for biochemical analyses and mineral assays. Serum Ca, P, ALP, Mg, Cu, Zn, Fe, Mn and Co levels were analyzed. Phosphorus and Zn levels were found to decrease significantly whereas Ca, ALP, Mn levels and Ca/P ratio found to increase before treatment. For treatment purpose, phosphoric acid and vitamin D-3 were added to their drinking water. Clinical findings improved after treatment and biochemical parameters which were abnormal before treatment returned to normal values after treatment. As a result, hypophosphatemia was one of the most important reasons of foot problems and bone fractures during the growth period of the turkeys. It was also observed that adding phosphorus and vitamin D-3 in the drinking water for the purpose of treatment was considered to be beneficial. (C) 2011 PVJ. All rights reserved
Effeciency of Various Supportive Treatments as a Cure for Anaemia in Cattle With Theileriosis
(Chulalongkorn Univ, 2014) Altug, Nun; Yuksek, Nazmi; Keles, Ihsan; Ozkan, Cumali; Yoruk, Ibrahim Hakki; Arslan, Sezai
The purpose of this study was to investigate the efficiency of various supportive treatments on the restoration of anaemia in theileriosis. A total of 20 cattle infected with theileriosis received intramuscularly a single dose of buparvaquone (2.5 mg/kg(-1) bw) and were divided into 4 equal groups according to the different therapy options: whereas no supportive treatment was applied in group 1, administrations of vitamin B complex, antioxidant vitamins (A, D, E and C), minerals and trace elements were performed in groups 2, 3 and 4, respectively. Haematological parameters, serum concentrations of antioxidant vitamins, minerals and trace elements as well as serum glutathion peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were determined before treatment and 7 days after in infected animals and compared to control values from 10 healthy cattle. In the theileriosis affected cattle, remarkable decreases in haematocrit values, haemoglobinaemia and platelet counts were associated to decreased serum alpha- / gamma-tocopherol, vitamin C, vitamin B-12, Ca, Mg, Zn, Na, GSH-Px and SOD levels and to increased sideraemia. The iron concentrations remained elevated and some antioxidants (alpha- / gamma-tocopherol and vitamin C concentrations and GSH-Px and SOD activities) decreased in all treated cattle. Haematocrit values, haemoglobinaemia and vitamin B-12 concentrations also remained depressed but maximal increases were recorded in cattle receiving vitamins B as supportive therapy (group 2). In parallel, platelet numeration was significantly restored in all groups, but specifically in group 2. On the other hand, the maximal increases in alpha-tocopherol and Zn concentrations recorded in groups 3 and 4, respectively, were not associated with cure of anaemia. These results show that the most efficient supportive therapy in the cure of anaemia during theileriosis was vitamin B administration.
Evaluation of Acute Phase Protein Levels and Some Cytokine Levels in Pneumonic Calves
(Polish Soc veterinary Sciences Editorial office, 2019) Akgul, Omer; Kozat, Suleyman; Ozkan, Cumali; Kaya, Abdullah; Akgul, Yakup
This study was carried out to determine the levels of various cytokines (TNF-alpha, IL-6, IL-8) and acute phase proteins (haptoglobin, C-reactive protein, serum amyloid A, alpha 1-acid glycoprotein, lactoferrin, and fibrinogen) in pneumonic calves, to reveal changes in these parameters depending on etiologic factors (bacterial, viral, bacterial + viral) in calf pneumonia, and to determine whether these parameters could be used for diagnosis and prognosis of the disease. The study was carried out on a total of 50 calves consisting of 10 healthy and 40 pneumonic animals aged 1 to 6 months. The pneumonic calves were classified into three groups: bacterial, viral and mixed (bacterial + viral). It was found that the levels of IL-6, IL-8, CRP, and TNF-alpha were statistically higher in the pneumonic calves than in the control group animals. It was found that the increase in levels of Serum IL-6, IL-8, and CRP were highest in the mixed infection group (bacterial + viral) and lowest in the virally infected group. Similarly, serum levels of Hp, CRP, SAA, alpha-1-AGP, and LF were found to be more statistically significant (P <0.01) in the infected calves than in the control group animals, but changes in fibrinogen levels were not statistically significant. Although the TP and ALB levels in the pneumonic calves were numerically higher than the same parameters in the control group, this was not statistically significant (P> 0.05). As a result, this study showed that serum levels of cytokines (IL-6, IL-8, TNF-alpha) and serum concentrations of acute-phase proteins (Hp, CRP, SAA, alpha-1-AGP, and LF) are significantly higher in the pneumonic calves than in the control group calves. Furthermore, the assessment of cytokine and acute phase protein levels can play an important role in the early diagnosis of calf pneumonia. This early diagnosis can help prevent deaths caused by pneumonia, which is the leading cause of major losses, and can also be helpful in the early treatment of pneumonia in the veterinary clinical field.
Evaluation of Homocysteine Levels in Neonatal Calves With Diarrhea
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Kozat, Suleyman; Ozkan, Cumali; Okman, Eda Nur
The aim of this study was to investigate the relationship between serum homocysteine (HCY), and creatinine, urea, venous blood gas and electrolytes values in neonatal calves with diarrhea. The study was conducted on a total of 30 calves, 20 with diarrhea and 10 healthy (control), with diarrhea complaints, of different races, sexes and ages ranging from 2-24 days. According to the venous blood gas results, the pCO(2) and base deficit values of calves with diarrhea were significantly higher (P<0.001) compared to the control group values, while pH, pO(2) and HCO3 values were significantly lower (P<0.001). While serum Na+ and Cl- concentrations in diarrheic calves did not show any statistical change when compared to the control group (P>0.05), serum K+ concentrations were statistically higher (P<0.001). Serum HCY, folate and vitamin B-12 concentration values of diarrheic calves were significantly higher (P<0.001) when compared to the control group. As a result; in neonatal calves with diarrhea, it has been concluded that homocystein excretion is disrupted by low renal excretion due to decrease in glomerular filtration rate that caused hyperhomocysteine. In addition, it is thought that this study will shed light on studies that will reveal the effect of hyperhomocysteinemia in the cardiovascular system in diarrheic calves.
Evaluation of the Levels of Homocysteine, Troponin I, and Nitric Oxide in Lambs With Subclinical White Muscle Disease
(Kafkas Univ, veteriner Fakultesi dergisi, 2011) Kozat, Suleyman; Altug, Nuri; Yuksek, Nazmi; Ozkan, Cumali
White muscle disease (WMD) or Nutritional myodegeneration disease (NMD) is a degenerative disease of the cardiac and skeletal muscles. The level of serum activity of Plasma homocysteine (Hcy), cardiac troponin I (cTn I), Nitric oxide (NO) were investigated in lambs with subclinical NMD in this study. Ten healthy lambs and twenty lambs with subclinical NMD were used in this study. The blood samples were firstly taken at the onset of the disease and then 3 and 10 days after treatment in NMD, and taken once control group. The values of serum activity of creatin kinase (CK), aspartate aminotranferase (AST), alanine aminotranferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were measured with autoanalyzer. Plasma concentration of Hcy, serum cTn I, and levels of NO were determined with ELISA method and value of activity erythrocyte glutathione peroxidase (GSH-Px) was measured with spectrophotometer. Before treatment, values of plasma Hcy, serum NO, cTn I, AST, ALT, ALP, CK, and LDH in lambs with NMD were higher than those of healthy ones (P<0.001), while GSH-Px activity of lambs with NMD are lower than those of healthy (P<0.001). After ten days of treatment, there were no significant differences between treated lambs with NMD and healthy lambs. Nutritional myodegeneration in lambs is associated with increased levels of Hcy, cTn I, and NO. Increased levels of Hcy, cTn I, and NO may be a result of selenium deficiency causing myocardial disorder in NMD lambs.
Immunohistochemical Diagnosis of Pseudorabies (aujeszky's Disease) in a Cow in Van, Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2017) Yildirim, Serkan; Ozkan, Cumali; Yener, Zabit; Cetin, Musa; Kozat, Suleyman
Aujeszky's disease, also known as pseudorabies, is caused by an alphaherpesvirus that infects the central nervous system and other system. Disease was first recognized as a fatal viral disease of cattle and dogs in 1902. The natural infections have been reported worldwide in swine, cattle, dogs and variety of wildlife species in more recent years. The study material was a 5 years old cow which was brought with symptoms such as skin itching, weakness, convulsions, fever and neurogical signs. As a result of the clinical examination the animal was suspected for Aujeszky's disease. Slaughter and necropsy were recommended. Subsequent to necropsy, tissues were fixed in formalin. Samples were evaluated histopathologically and immunohistochemically. According to the histopathological findings; diffuse, non-suppurative inflammation of brain, spinal cord and spinal nerves were determined. Brain lesions were detected commonly in cerebral and cerebellar cortexes. Grey and white matter were both affected. It was observed marked perivascular lymphoplasmocytic cell infiltration, glial cell proliferation, Hyperemia and hemoragia in veins in brain and light chromatolysis have been identified in some neurons. Intranuclear inclusions were observed in neurons. On the skin itching region of animal, serofibrinous inflammation in the dermis and subcutis was detected. According to the immunohistochemical findings; immunoperoxidase was detected strong positive in cytoplasms of both neurons and glial cells in areas with meningitis in brain. Consequently; after clinical, macroscopical and microscopical examination, this case was diagnosed as Aujeszky's disease. The aim of this study is presenting this disease immunochemically as it has never been reported in cattle in Turkey. We believe that it would be appropriate to present this case report since it is the first Pseudorabies case in cattle in Turkey.
Measurement of M-Mode Echocardiographic Parameters in Healthy Adult Van Cats
(Hokkaido Univ, 2014) Kayar, Abdullah; Ozkan, Cumali; Iskefli, Onur; Kaya, Abdullah; Kozat, Suleyman; Akgul, Yakup; Or, Mehmet Erman
Cardiomyopathies are the most common type of cardiac diseases in cats. Although some normal echocardiographic values for cats have been published, there are variations based on breeds and gender. The objective of this study is to determine normal reference values for M-mode echocardiographic parameters in nonsedated healthy adult Van cats and to compare those values with data reported for nonsedated healthy cats of other breeds. A total of 40 clinically healthy Van cats of both sexes belonging to the Van Cat Research and Application Center of Yuzuncu Yil University were used. Body weight (BW) and 16 M-mode echocardiographic variables were measured in 40 healthy Van cats. The effect of gender and age on each echocardiographic parameter was analyzed and the relationship between BW and each parameter investigated. There was a significant relationship between gender and left atrial dimension during ventricular systole (LAD) and aortic root dimension at end-diastole (AOD) as well as between BW and interventricular septal thickness at end-diastole (IVSd) and end-systole (IVSs), left ventricular internal dimension at end-diastole (LVIDd), left ventricular posterior wall thickness at end-diastole (LVPWd), LAD, AOD, the left ventricular end diastolic volume (EDV) and the stroke volume (SV). A relationship between age and the SV parameter alone was also established. This present study is the first work on cardiac reference values for Van cats highlighting the differences in some M-mode echocardiographic parameters of healthy adult Van cats and other cat breeds, which should be considered when interpreting echocardiographic findings, in order to draw the correct conclusions regarding cardiac health.
Medical and Surgical Treatment of Gastric Impaction in Juvenile Ostriches
(Medwell online, 2009) Aslan, Logman; Karasu, Abdullah; Ozkan, Cumali; Duz, Erkan; Kaya, Abdullah; Akgul, Yakup
Radiological diagnosis and medical and surgical treatment options of the constipation problem seen in ostrich chicks were investigated in this study. The study was performed in a farm that 60 ostrich chicks were kept that are between 8 and 12 weeks of age. Our clinic was applied to upon seeing loss of appetite, weight loss and sudden death in 2 animals in the farm. Fifteen sick ostrich chicks that gastric impaction problem was found with clinical and radiological examination were included in the study. In the ostrich chicks that were found to be sick in clinical examination, clinical symptoms were observed like loss of appetite, weakness, dehydration, reduced number of defecations, drooping wings, messiness of feathers and inability to stand. Medical treatment was applied to 7 animals that general health status was not severe and that obstruction was found to be not complete in radiological examination and proventriculotomy operation was performed in 8 animals that stomachs were found to be full with foreign materials in radiological examinations and with serious health condition and also in 2 ostrich chicks that did not respond to medical treatment. Five patients out of 7 that medical treatment was applied and 8 patients out of 10 that were treated surgically responded to the treatment and got well. In the autopsies of the animals that did not respond to the treatment and died, macroscopic edema, erosion and hemorrhagic ulcers were found in proventriculus and ventriculus mucosa and in addition, stomach was filled with stones, sand, pieces of wood, metal and glass, rough feed particles and clover fibers. It was concluded that radiological examination will determine the level of obstruction, make the treatment easier and will direct it. It was also, concluded that emergency surgical treatment in cases with complete obstruction not responding to medical treatment would be beneficial if performed with no time loss.
Parasites Detected in Neonatal and Young Calves With Diarrhoea
(Natl veterinary Research inst, 2006) Goz, Yasar; Altug, Nuri; Yuksek, Nazmi; Ozkan, Cumali
This study was performed between December 2000 May 2005 to detect parasitic diarrhoea agents in neonatal and young calves. In this period, faecal samples were obtained from 231 neonatal and young calves. Age of the calves ranged from I day to 8 months. Faecal samples were examined using native Lugol's solution, Fulleborn's salty water technique and modified Ziehl-Neelsen staining technique for Cryptosporidium. The parasitological examination revealed that 188 (81.3%) of the calves were infected with one or more parasitic agents.
Prevalence of Four Enteropathogens With Immunochromatographic Rapid Test in the Feces of Diarrheic Calves in East and Southeast of Turkey
(Univ Agriculture, Fac veterinary Science, 2013) Icen, Hasan; Arserim, Neval Berrin; Isik, Nurettin; Ozkan, Cumali; Kaya, Abdullah
In this study, fecal specimens taken from 192 diarrheic and 14 healthy calves (2-40 days old) were examined for the presence of bacterial and parasitic agents. Fecal samples from diarrheic calves with the four immunochromatographic rapid tests were 92.7% positive for four enteropathogens. The individual prevalence was 25, 21.8, 9.4 and 2.1% for Rotavirus, Cryptosporidium parvum, E. coli K99 and Coronavirus, respectively. Concomitant infections caused by two agents were 15.6% for Rotavirus+Cryptosporidium, 1.0% for Rotavirus+Coronavirus, 5.2 % for Cryptosporidium+E. coli K99, and 7.3% for Rotavirus+E. coli K99. Besides concomitant infections caused by three agents were 3.1% for Cryptosporidium +Rotavirus+E. coli K99 and 1.0%, Cryptosporidium+Rotavirus+Coronavirus. In addition one calf (1.0%) was infected by combination of four agents as Cryptosporidium, Rotavirus, Coronavirus, and E. coli K99. The calculated individual prevalence was 56.9% for Rotavirus, 47.8% for C. parvum, 26.0% for E. coli K99 and 5.2% for Coronavirus. However, 88 samples were positive in smear detection for Cryptosporidium while 92 were positive in rapid test. As a result of this study it can be concluded that multiple etiologies of diarrhea can be seen and this can help in the development of a specific treatment and preventative measures for practitioners in east and southeast of Turkey. (C) 2013 PVJ. All rights reserved
Relationship Between Packed Cell Volume Levels and Serum Nitric Oxide Concentrations in Cattle With Tropical Theileriosis
(Univ Agriculture, Fac veterinary Science, 2015) Ozkan, Cumali; Altug, Nuri; Akgul, Yakup; Keles, Ihsan; Kaya, Abdullah; Yuksek, Nazmi; Yur, Fatmagul
This study was performed to determine changes in serum NO (nitric oxide) levels in cattle with theileriosis in accordance with PCV levels. For this purpose, 42 cattle with theileriosis and 10 healthy cattle have been used as material. Infected animals have been divided into 3 equal groups. 1st group had <12%, 2nd group had 13-24% and 3rd group had <25% PCV level. Blood samples have been used to determine NO concentrations. Serum NO concentrations prior to treatment increased in all infected groups compared to control group however its levels were significant only in the third group. NO concentrations decreased to almost normal values after treatment especially in 2nd and 3rd group, however this decrease was not significant in the first group. As a result, NO levels have been found increased in cattle with natural theileriosis. Therefore, its concluded that NO level and PCV levels in theileriosis should be studied with more detail in the future to enlighten the role of NO release and strategies for controlling its' release should be made especially in severe cases. (C)2015 PVJ. All rights reserved
Rupture of the Gastrocnemius Muscle in a Cow Two Months After Twin Birth
(Natl veterinary Research inst, 2007) Altug, Nuri; Ozkan, Cumali; Yuksek, Nazmi; Karasu, Abdullah; Keles, Ihsan; Agaoglu, Zahid Tevfik; Ilhan, Fatma
The rupture of the gastrocnemius muscle was described in a cow who gave a twin birth. A tentative diagnosis of complete rupture of the muscle was based on information obtained from both anamnesis and clinical signs. The definitive diagnosis was made on necropsy. The role of serum minerals, especially Ca, changes in the muscle originated enzymes, and other laboratory parameters together with clinical examination findings was discussed.
Selected Tumour Biomarker Levels in Sheep With Pulmonary Adenomatosis
(Sciendo, 2020) Ozkan, Cumali; Yildirim, Serkan; Huyut, Zubeyir; Ozbek, Mustafa
Introduction: Sheep pulmonary adenomatosis (ovine pulmonary adenomatosis, OPA, Jaagsiekte) is a chronic contagious bronchoalveolar carcinoma caused by the Jaagsiekte sheep retrovirus. Since effective treatment and a vaccination procedure are not currently possible, control and eradication of the disease is difficult. It leads to serious economic losses around the world, therefore studies are currently underway in order to design control and eradication programmes. In this study, levels and changes in selected tumour markers (carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 125, CA 19-9, CA 15-3, and alphafetoprotein (AFP)-3) and their diagnostic significance were investigated. Material and Methods: A total of 30 sheep were used. Clinical examinations were performed and blood samples were obtained before slaughter from all animals with presumed OPA. Blood samples with positive OPA results by macroscopic and histopathological examination were included in the study as the experimental group and numbered 20. Sheep totalling 10 had negative OPA results and provided control samples. Results: CEA levels were similar in both groups, and the differences were statistically insignificant (P > 0.05). CA 125, CA 19-9, CA 15-3, and AFP-3 levels were higher in the OPA group than the control group and with statistical significance (P < 0.05). In all OPA animals, CA 125 levels were higher than 1 U/mL. Conclusion: Serum CAs and AFP levels increase significantly in adenomatous sheep. These tumour markers are thought to facilitate the diagnosis of OPA.
Serum Homocysteine, Vitamin B12 and Folate Levels in Healthy Turkish Van Cats
(Network veterinarians Bangladesh, 2017) Ozkan, Cumali; Kozat, Suleyman; Kaya, Abdullah; Akgul, Yakup
Objective: The present study was conducted for the purpose of setting forth the normal serum Hcy, vitamin B-12 and folate levels in Van cats of varying ages and genders, and the age-dependent variations of these parameters. Materials and methods: The material of the study consisted of a total of 60 healthy Van cats including 30 female and 30 male cats. Cats from both genders were separated into 3 groups on the basis of their ages. While the cats of 6 - 12 months of age were included in the first group, cats of 12-24 months of age were included in the second and those of more than 24 months of age were included in the third group. Results: From the blood samples collected; serum normal homocysteine, vitamin B-12 and folate levels were determined as 7.1 +/- 2.2 nmol/mL, 850.7 +/- 231.8 pg/mL and 16.7 +/- 0.8 ng/mL, respectively. In the statistical comparison of the determined serum homocysteine, vitamin B-12 and folate levels; some variations among different groups of age and genders were determined. However, none of these differences were determined to be statistically significant. Conclusion: The normal levels of serum Hcy, vitamin B-12 and folate of healthy Van cats were set forth for the first time by the present study. It is believed that the normal values of these parameters in Van cats can be used in the diagnosis and prognosis of various diseases and particularly cardiovascular diseases, that they will be helpful for researchers and will serve as a guideline to the studies to be conducted in the future.
Serum Malondialdehyde, Coenzyme Q10 and 8-Hydroxy Levels in Calves With Foot-And Disease
(Polish Soc veterinary Sciences Editorial office, 2018) Ozkan, Cumali; Huyut, Zubeyir; Yildirim, Serkan; Ozbek, Mustafa; Icen, Hasan
Foot-and-mouth disease (FMD) is an acute, contagious viral disease in cattle that is associated with enormous economic losses in Turkey and worldwide. The purpose of this study was to determine changes in serum malondialdehyde (MDA), coenzyme Q10 (CoQ10), 8-hydroxy-2-deoxyguanosine (8-OHdG) and deoxyguanosine (dG) and to perform histopathological examinations in calves with FMD. Thirty calves were studied, 20 of which were infected with FMD and 10 were free of the disease. Following a routine clinical examination, blood samples were obtained, and serum MDA, CoQ10, 8-OHdG and dG levels were determined. Necropsy and histopathological examinations were performed on dead calves with FMD. MDA and 8-OHdG/ 106dG levels were significantly higher in calves with FMD than in the control group. However, the increase in CoQ10 levels in calves with FMD, compared with the control group, was not statistically significant. Macroscopic examination of the heart tissue of calves with acute myocarditis revealed the presence of pale, yellowish gray-white necrotic muscle fibers in the ventricular wall of the heart. The muscle fibers in the myocardium were swollen and exhibited pyknotic nuclei and intense lymphocytic cell infiltration. In longitudinal sections, the muscle fibers were non-striated, swollen, and homogenously pink and contained pink nuclei. Between muscle fibers, intense mononuclear cell infiltration was observed. The findings of the present study indicate that oxidative stress is significantly increased in calves with FMD, and that oxidative DNA damage may play an important role in the etiopathogenesis of FMD. This is the first study to report CoQ10 and 8-OHdG levels in calves with FMD, and its findings may serve as the basis for future studies on this subject.