Browsing by Author "Ozkan, Hakan"

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Anti-Mutagenic and Anti-Oxidant Potencies of Cetraria Aculeata (Schreb.) Fr., Cladonia Chlorophaea (Florke Ex Sommerf.) Spreng. and Cetrelia Olivetorum (Nyl.) W.l. Culb. & C.f. Culb.)
(Shaheed Beheshti Univ, Sch Pharmacy, 2018) Ceker, Selcuk; Orhan, Furkan; Sezen, Selma; Gulluce, Medine; Ozkan, Hakan; Aslan, Ali; Agar, Guleray
In this study, the mutagenic and anti-mutagenic effects of methanol extract of three lichen species (Cetraria aculeata, Cladonia chlorophaea and Cetrelia olivetorum) were investigated by using E. coli-WP2, Ames-Salmonella (TA1535 and TA1537) and sister chromatid exchange (SCE) test systems. The results obtained from bacterial test systems demonstrated that methanol extracts of three lichen species have strong anti-mutagenic potencies on TA1535, TA1537 strains and to a lesser extent on E. coli-WP2 strain. The anti-oxidant level of human lymphocytes cells was determined in order to clarify the mechanism underlying the anti-mutagenic effects of these lichen species. Co-treatments of 5, 10 and 20 mu g/mL concentrations of these three lichen species with AFB decreased the frequencies of SCE and the level of MDA and increased the amount of SOD, GSH and GPx which decreased by aflatoxin. The findings of this work have clearly demonstrated that Cetraria aculeata, Cladonia chlorophaea and Cetrelia olivetorum have significant anti-mutagenic effects which are thought to be partly due to the anti-oxidant activities and the interaction capability of lichen extracts with mutagen agents (Sodium azide, acridin, N-methyl-N'-nitro-N-nitrosoguanidine and aflatoxin B-1).
Dna Based Ipbs-Retrotransposon Markers for Investigating the Population Structure of Pea (Pisum Sativum) Germplasm From Turkey
(Pergamon-elsevier Science Ltd, 2015) Baloch, Faheem Shehzad; Alsaleh, Ahmad; Saenz de Miera, Luis E.; Hatipoglu, Rustu; Ciftci, Vandettin; Karakoy, Tolga; Ozkan, Hakan
Retrotransposons have been highly studied in monocots; however retrotransposon diversity in dicot crops has not been well documented. Our objective was to assess the diversity harbored by field pea landraces using retrotranposon markers. In this research, molecular characterization of 104 landraces and 34 field pea breeding lines was assessed using newly developed iPBS-retrotransposon markers. The 12 iPBS-retrotransposon primers generated a total 106 scorable bands, and 81 of these were found to be polymorphic (76.4%), with an average of 6.75 polymorphic fragments per primer. Polymorphism information content (PIC) ranged from 0.33 to 0.84 with an average of 0.61. It was evident that field pea landraces from the same geographical region were often placed in different groups in the neighbor joining analysis, indicating that grouping based on genetic parameters was not closely related to the geographical origin. The population structure was determined by using STRUCTURE software, and three populations at K = 3 and five populations at K = 5 were identified among landraces. The plentiful diversity present in Turkish field pea landraces could be used as genetic resource in designing breeding program, and may also contribute to worldwide pea breeding programs. Our data also suggested a role of iPBS-retrotransposons as 'a universal marker' for molecular characterization of pea germplasm. (C) 2015 Elsevier Ltd. All rights reserved.
Molecular Characterization of Thermophilic Bacteria Isolated From Van City Ercis Town Hasanabdal Hot Spring
(Ars Docendi, 2009) Savas, Sumeyra; Adiguzel, Ahmet; Inan, Kadriye; Ozkan, Hakan; Gulluce, Medine; Sahin, Fikrettin
The study was carried am in order to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Van city Ercis town Hasanabdal hot spring water. Turkey Fatty Acid, rep PCR profiling methods, and 16S rDNA sequence data were used for the molecular characterization of thermophilic bacteria, Totally 9 different bacterial isolates were selected based on morphological, physiological and biochemical tests. These strains were further characterized by molecular tests including fatty acid and rep PCR profiles, and 16S rDNA sequence. Its the Fatty Acid profiles were examined, it was determined that for P13 and P9 isolates, iso-C15:0. anteiso-C15:0. iso-C17:0 and anteiso-C17:0 fatty acids are the major fatty acids. For P22, P86, P56. P60. P59 and P4 isolates, the major fatty acids are C15:0. iso-C16:0, and iso-C17:0 fatty acids, and the major fatty acids for P14, P35 isolates are iso-C15:0 and anteiso-C15:0 fatty acids. These bacterial strains that differ in terms of fatty acid profiles were analyzed by REP and BOX-PCR, which are a genomic fingerprint analysis method. For the strains, which exhibited differences with respect to each other at the end of this analysis, 16S rDNA analyses were performed. As a result of 16S rDNA analyses, it was observed that six of these isolates were identified as Geobacillus pallidus and remaining four strains were Bacillus licheniformis, B. pumilus, Brevibacillus brevis and Br. borstelensis species respectively.