Browsing by Author "Ozkan, Huseyin"

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Alterations in Oxidative Stress Markers in Laryngeal Carcinoma Patients
(Lippincott Williams & Wilkins, 2018) Bozan, Nazim; Demir, Halit; Gursoy, Tulay; Ozkan, Huseyin; Duzenli, Ufuk; Sankaya, Eren; Cankaya, Hakan
Background: Data describing how laryngeal cancer affects oxidative stress markers and antioxidants are limited. This study investigated serum antioxidant enzyme activities and oxidative stress markers before and after laryngectomies in patients with laryngeal cancer. Methods: A total of 29 patients with laryngeal cancer and 25 healthy control subjects were enrolled. Serum malondialdehyde (MDA) levels and superoxide dismutase (SOD), glutathione peroxidase (GSHPx), catalase (CAT), paraoxonase (PON), and arylesterase activities were measured spectrophotometrically. Blood samples were obtained from each patient just before surgery and 1 month after a laryngectomy. Results: The serum PON, arylesterase, CAT, SOD, and GSHPx activities were significantly decreased (all p < 0.001) and serum MDA levels were significantly increased (p < 0.001) in patients with laryngeal cancer, compared with control subjects. In laryngeal cancer patients, the serum GSHPx and arylesterase activity levels increased significantly following laryngectomies (both p < 0.001), whereas the MDA levels decreased significantly (p = 0.007). Conclusion: In patients with laryngeal cancer, the oxidant/antioxidant balance shifted toward oxidative stress. In addition, following laryngectomies, laryngeal cancer patients had increases in serum antioxidant enzyme activities and decreases in oxidative stress markers. Copyright (C) 2018, the Chinese Medical Association. Published by Elsevier Taiwan LLC.
An Investigation of 8-Hydroxy and 8-Iso F2α Levels in Patients With Larynx Carcinoma
(dergipark Akad, 2020) Cetin, Yaser Said; Duzenli, Ufuk; Berkoz, Mehmet; Ozkan, Huseyin; Bozan, Nazim
Objective: 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-iso-prostaglandin F-2(alpha) (8-iso-PGF(2)(alpha)) are indicators of oxidative stress in the human body. The aim of the present study was to investigate serum 8-OHdG and 8-iso-PGF(2)(alpha) levels in patients with larynx carcinoma and to compare them with healthy controls. Methods: A total of 50 patients, consisting of 25 patients with larynx carcinoma (study group) and 25 healthy subjects (control group), were enrolled in the present study. Serum 8-OHdG concentration was measured using an ELI-SA kit, whereas an enzyme immunoassay kit was utilized for the measurement of serum 8-iso-PGF(2)(alpha) levels. TNM stages of the patients were recorded from the patient records in the study group. Results: 8-OHdG and 8-iso-(2)(alpha) levels were significantly higher in patients with larynx carcinoma than the control subjects. 8-OHdG levels were significantly higher in patients with LN metastases than the patients without LN metastases in the study group. Conclusion: The findings of the present study showed the presence of significantly increased serum 8-OHdG and 8-iso-PGF(2)(alpha) levels in patients with larynx carcinoma, which may support the role of oxidative stress in the development laryngeal cancers.
Klippel-Feil Syndrome and Associated Ear Anomalies
(W B Saunders Co-elsevier inc, 2008) Yidirim, Nadir; Arslanoglu, Atilla; Mahirogullari, Mahir; Sahan, Murat; Ozkan, Huseyin
Background and purpose: Klippel-Feil syndrome (KFS) is a congenital segmentation anomaly of the cervical vertebrae that manifests as short neck, low hair line, and limited neck mobility. Various systemic malformations may also accompany the syndrome including wide variety of otopathologies affecting all 3 compartments of the ear (external, middle, and inner ear) as well as internal acoustic canal and vestibular aqueduct. We aimed to investigate these involvements and their clinical correlates in a group of patients with KFS. Materials, methods, and results: We present 20 KFS cases, of which 12 (% 60) displayed most of the reported ear abnormalities such as microtia, external ear canal stenosis, chronic ear inflammations and their sequels, anomalies of the tympanic cavity and ossicles, inner car dysplasies, deformed internal acoustic canal, and wide vestibular aqueduct, which are demonstrated using the methods of otoscopy, audiologic testing, and temporal bone computed tomography. Conclusions: This series represents one of the highest reported rate of ear involvement in KFS. We found no correlation between the identified ear pathologies and the skeletal and extraskeletal malformations. The genetic nature of the syndrome was supported by the existence of affected family members in 4 (20%) of the cases. (C) 2008 Elsevier Inc. All rights reserved.
The Protective Effect of Esculetin Against Aluminium Chloride-Induced Reproductive Toxicity in Rats
(Wiley, 2021) Turk, Erdinc; Ozan Tekeli, Ibrahim; Ozkan, Huseyin; Uyar, Ahmet; Cellat, Mustafa; Kuzu, Muslum; Guvenc, Mehmet
One of the prominent health problems caused by Aluminium was the decrease in male fertility rates. In the study, the protective effect of Esculetin (ESC) against the reproductive toxicity induced by Aluminium chloride (AlCl3) was investigated. For this purpose, AlCl3 was administrated to Wistar Albino rats at a dose of 34 mg/kg and ESC was administrated at a dose of 50 mg/kg for 70 days. It was determined that AlCl3 treatment reduced sperm motility and concentration, increased dead/live rate and abnormal sperm rate. It decreased serum testosterone level, and co-treatment of ESC significantly regulated these values. In the AlCl3-treated group, MDA level increased and GSH level, GPx and CAT activities decreased compared with those of the control group. However, co-treatment of ESC showed an amelioratory effect on the values except for CAT activity. It was observed that the expression level of NRF-2 increased in the ESC and AlCl3 + ESC groups, and NF-kappa B increased in the AlCl3 group with the control group. It was determined that Caspase-3 expression decreased, and Bcl-2 expression increased in AlCl3 + ESC group compared to AlCl3 group. It was also determined that AlCl3-induced tissue injury was significantly prevented by ESC co-treatment.