Browsing by Author "Ozok, Necati"

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Anti-Hyperglycemic Effect of Vicia Ervilia (L.) Willd (Fabaceae) Seed Extract and Its Effect on Lipid Profile, and Hepatic and Renal Biomarkers in Streptozotocin-Induced Diabetic Rats
(Pharmacotherapy Group, 2019) Kartal, Derya; Ozok, Necati
Purpose: To evaluate the impact of Vicia ervilia (L.) Willd (Fabaceae) lyophilized extract on various biomarkers of streptozotocin-induced diabetic rats. Methods: Adult male rats (36) were divided into 6 groups, viz, normal control (NC), diabetes control (DC), and treatment groups. Experimental diabetes was induced with streptozotocin. Blood samples were taken from the tail veins daily for 25 days. Fasting and postprandial blood glucose levels of rats were determined. The rats were sacrificed and blood, kidney and liver were taken for biochemical analysis. Results: Blood glucose decreased in DC groups compared to NC group (p <= 0.05). In diabetic extract groups, glycated Hb decreased compared to DC group (p <= 0.05). Alpha glucosidase activity was reduced in all treatment groups compared to DC group (p <= 0.05). Only triglyceride levels of diabetes extract (400 mg/mL) group decreased compared to DC (p <= 0.05). Total cholesterol levels fell compared to NC and DC groups (p <= 0.05). Low density lipoprotein was lower compared to NC (p <= 0.05), while aspartate aminotransferase and alanine aminotransferase levels rose in diabetic extract groups compared to NC, but fell in some diabetic extract groups compared to DC group (p <= 0.05). Lactate dehydrogenase decreased in all diabetic extract groups compared to NC and DC (p <= 0.05). Serum urea and creatinine levels were higher in diabetic extract groups compared to NC, but decreased in DC group (p <= 0.05). Conclusion: These findings demonstrate that Vicia ervilia lyophilized extract haas positive effects on blood glucose and biochemical parameters in diabetic rats compared to DC of V. ervilia seed extract.
Effects of 4-Chlorophenoxy Acetic Acid on Oxidative Stress and Antioxidant Enzymes in Primary Hepatocyte Cell Culture in Lake Van Fish (Alburnus Tarichi Guldenstadt 1814)
(Parlar Scientific Publications (p S P), 2020) Ozok, Necati; Oguz, Ahmet R.
Synthetic plant regulators are used extensively and uncontrollably in order to increase agricultural production. The 4-chlorophenoxyacetic acid used for this purpose is contaminating soil and water, and leaving residue on the products. Therefore, it cannot be ruled out that there may be adverse effects on nontarget organisms and especially on animal health. In this study, the effects of oxidative stress and antioxidant enzymes were investigated in the primary hepatocyte culture of Lake Van fish (Alburnus tarichi, Guldenstadt 1814) exposed to 4-chlorophenoxyacetic acid. Statistically significant reductions in superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase enzyme activities and reduced glutathione levels were observed in comparison with the obtained findings in the control group (P <= 0.05). There was also a significant increase in the level of malondialdehyde, a product of lipid peroxidation (P <= 0.05). As a result, it was determined that Lake Van fish treated with different concentrations of 4-chlorophenoxyacetic acid exhibited oxidative stress and lipid peroxidation in the primary hepatocyte cell culture.
Effects of Chlorpyrifos on Primary Gill Cell Culture of Lake Van Fish (Alburnus Tarichi Guldenstaadt 1814)
(Oxford Univ Press, 2020) Oguz, Ahmet Regaib; Oguz, Elif Kaval; Ozok, Necati
Lake Van fish (Alburnus tarichi Guldenstadt 1814) is the only fish that can adapt to the extreme conditions (pH 9.8 salinity 0.2% and alkalinity 151.2 meq/L) of Lake Van. In this study, it was aimed to determine the cytotoxic and genotoxic effects of chlorpyrifos (CPF) on Lake Van fish primary gill cell culture. Gill epithelium from Lake Van fish was isolated enzymatically and grown in primary culture on Leibovitz's L-15 medium. After different doses (0.01, 0.1, 1, and 10 mu M) of CPF were applied to the gill cells, the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA), and DNA damage levels (8-hydroxyguanine (8-OHdG)) were examined at the end of 24 and 48 h. It was determined that the TOS, MDA, and 8-OHdG levels increased in the cells exposed to high doses (1 and 10 mu M) of CPF and the TAS was decreased (P < 0.05). It was revealed from this study that CPF administered at a dose higher than 1 mu M can cause oxidative stress and DNA damage in the primary gill cell culture of Lake Van fish. In addition, the findings showed that Lake Van fish primary gill cell culture was useful in determining the effects of toxic substances likely to be the contaminants of a lake. [GRAPHICS]
Effects of Cypermethrin on Antioxidant Enzymes and Lipid Peroxidation of Lake Van Fish (Alburnus Tarichi)
(Taylor & Francis Ltd, 2020) Ozok, Necati
Lake Van fish is the only vertebrate species that lives in extreme condition (salinity of 2.2%, alkalinity of 151.2 meq/kg, and pH of 9.8) of largest soda lake of the world. This fish is an important source of protein for the people living in Van and surrounding provinces. . Cypermethrin (CYP) is used extensively against insects in agricultural, livestock, and domestic applications in Lake Van basin. CYP has toxic effects highly on non-target organisms as aquatic animals. The aim of this study was to evaluate the effect of commercial formulas of CYP (0.05 mu g/L) on Van Lake fish (Alburnus tarichi) for 24, 48, 72, 96 h under semi-static conditions using oxidative damage biomarkers. In this study, antioxidant defense system enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities and lipid peroxidation (LPO) product malondialdehyde (MDA) levels were determined in the liver, kidney, gills, and brain tissues. According to the results of this study, the decrease in SOD (at 48, 72, and 96 h) and CAT and GPx (at 24, 48, 72, and 96 h) enzyme activities were significant with exposure to CYP (p < 0.05). In addition, the increase in MDA levels, which is the indicator of LPO, was found to be significant (p < 0.05). However, in the 24 h period exposed to CYP, a significant increase in SOD activity was determined in kidney and brain compared to the control group (p < 0.05). In conclusion, sublethal concentrate application of CYP is thought to cause oxidative stress and disrupt the oxidant, antioxidant balance of Lake Van fish.
Hemato-Biochemical Responses of Van Fish (Alburnus Tarichi Guldenstadt, 1814) During Sublethal Exposure To Cypermethrin
(Taylor & Francis inc, 2018) Ozok, Necati; Oguz, Ahmet R.; Kankaya, Ertugrul; Yeltekin, Asli Cilingir
Cypermethrin is a highly active type II pyrethroid that is widely used to control harmful insects. The present study was conducted to investigate the possible effects of the sublethal concentration of cypermethrin (0.05L/L) on the biometric, hematological, and biochemical parameters of Van fish (Alburnus tarichi Guldenstadt 1814) for 24, 48, 72, and 96h. Behavioral and physiological changes were observed in the fish treated with cypermethrin. Hematological parameters revealed a significant decrease in the erythrocyte (RBC), hemoglobin (Hb), and hematocrit (Hct) values (p .05). Serum enzymes, aspartate aminotransferase (AST, EC 2.6.1.1), alanine aminotransferase (ALT, EC 2.6.1.2), and lactate dehydrogenase (LDH, EC 1.1.1.27) activities and serum cortisol level were significantly increased (p .05). The results showed that changes in the biometric, biochemical, and hematological values of the Van fish exposed to cypermethrin can be used as biomarkers for monitoring toxicity.
Investigation of the Therapeutic Effect of Melatonin on Deltamethrin Applied Mouse Primary Hepatocyte Culture
(Taylor & Francis Ltd, 2025) Kaval Oguz, Elif; Oguz, Ahmet Regaib; Ozok, Necati; Alkan, Zehra; Ergoz Azizoglu, Burcu; Orgi, Elif; Yesilbas, Ayse
ObjectiveIn recent years, it has been known that the melatonin hormone, secreted from the pineal gland, possesses significant antioxidant activity. This study explores the therapeutic effect of melatonin on the deleterious effects of deltamethrin, a pyrethroid pesticide extensively used worldwide, including in T & uuml;rkiye, on mouse liver cells.MethodsHepatocytes from Balb/C mice were isolated using a two-stage perfusion method, resulting in over 85% live hepatocytes. The isolated cells were cultured with different doses of deltamethrin (1 and 10 mu M) and melatonin (100 mu M) for 24 and 48 hours. At the conclusion of the culture period, hepatocytes were extracted at the 24th and 48th hours, and Malondialdehyde (MDA), Total Antioxidant Capacity (TAC), Total Oxidation Status (TOS), and DNA damages (8-hydroxy-2'-deoxyguanosine (8-OHdG)) were examined.ResultsWhile an increase in MDA, TOS, and DNA damage was observed in the deltamethrin-administered groups of hepatocytes, a decrease in TAC level was noted. It was determined that the applied deltamethrin had no effect on cell viability throughout the application period.ConclusionFurthermore, it was observed that melatonin, when administered concurrently with deltamethrin, reduced the toxic effect of deltamethrin. This study suggests that melatonin has a protective effect against deltamethrin-induced damage in mouse hepatocyte cells.
Neurotoxic and Immunotoxic Effects of Fenthion and Omethoate on Frogs at Acute Exposure
(Sage Publications inc, 2011) Celik, Ismail; Isik, Ismail; Ozok, Necati; Kaya, M. Salih
The study was carried out to investigate the neurotoxic and immunotoxic effects of fenthion-and omethoateused agricultural areas on frogs (Rana ridibunda) at acute exposure. The neurotoxic effects of the chemicals were evaluated by measuring the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Biomarkers selected for immunotoxic monitoring were the activities of adenosine deaminase (ADA) and myeloperoxidase (MPO) in various tissues of frogs exposed to 10 or 20 parts per million (ppm) dosages of fenthion and omethoate for 24, 48, 72 and 96 hours. Results showed that the administrations of chemicals fluctuated AChE and BChE activities in some tissues of frogs treated with both dosages at all the periods. With regard to the immunotoxic effects, MPO activity was increased in almost all the tissues of frogs after 10 and 20 ppm dosages and for 24, 48, 72 and 96 hours exposure of fenthion and omethoate as compared to those of control whereas ADA activity did not change in all the tissues. This may reflect the potential role of these parameters as useful biomarkers for toxicity of fenthion and omethoate.