Browsing by Author "Ozturk, Oznur"

Now showing 1 - 3 of 3

Evaluation of Pulp Tissue Dissolving Efficiency of Sodium and Calcium Hypochlorite Solutions Activated by Ultrasonics and Laser: an in Vitro Study
(Bmc, 2024) Ozturk, Oznur; Sen, Ozgur Genc
ObjectivesThis study aimed to compare the tissue dissolving capacities of sodium and calcium hypochlorite [NaOCl and Ca(OCl)2] solutions applied without activation or with two irrigant activation methods.Materials and MethodsOne hundred and eight dentin cavities were prepared. Preweighed tissue pieces were placed in these dentin cavities, and samples were divided into nine groups of twelve. In groups 1, 2, and 3, NaOCl was used with non-activation (NA), passive ultrasonic (PUA), and laser activation (LA). With the same techniques, Ca(OCl)2 was used in groups 4, 5, and 6, and distilled water was used in groups 7, 8, and 9. The weight loss of tissue samples was calculated and analyzed using Two-way ANOVA and Duncan tests.ResultsDistilled water groups showed no tissue dissolution in any conditions. NaOCI and Ca(OCI)2 showed statistically similar dissolving effectiveness when used with the same technique. Activated groups dissolved significantly greater tissue than non-activated ones, the highest in LA.ConclusionsCa(OCI)2 can be an alternative to NaOCl; for both, the first choice of activation may be the LA.
Evaluation of Western Blot Method for the Detection of Antibodies To Helicobacter Pylori Antigens in Patients With Gastric Carcinoma and Cases With Epigastric Complaints
(Ankara Microbiology Soc, 2010) Guducuoglu, Huseyin; Berktas, Mustafa; Bozkurt, Hamza; Ozer, Turkan Toka; Bulut, Guelay; Ozturk, Oznur; Ilhan, Mahmut
Helicobacter pylori proteins CagA (cytotoxin-associated gene A) and VacA (vacuolating cytotoxin A) are among the virulence factors of this species. CagA gene carrying H.pylori strains are particularly associated with gastric adenocarsinoma. This study was conducted to evaluate Western Blot (WB) method to determine specific H.pylori antibodies in a group of patients with gastric cancer and in a control group with no malignancy. A total of 99 patients with gastric cancer (94 adenocarcinoma, 2 adenosquamous cell carcinoma, 3 non-Hodgkin lymphoma) and 150 control cases with epigastric complaints such as nausea, vomiting, diarrhea, gastroesophageal reflux and abdominal pain, were included to the study. H.pylori IgG-ELISA was positive in all study (mean age: 56.7 +/- 1.2 years, 62 male) and control (mean age: 24.2 +/- 1.3 years, 64 male) patients. Specific antibodies against CagA, VacA, OMP (outer membrane protein)-67, urease-A, urease-B, HSP (heat shock protein) and flagellin antigens determined by a commercial WB-based kit (RIDA Blot Helicobacter, R-Biopham GmbH, Germany). Interestingly, no anti-VacA positivity was detected in none of the patient and control groups. The positivity rates for H.pylori CagA, OMP-67, urease A, urease-B, flagellin and HSP specific antibodies were as 78%, 54%, 37%, 60%, 53% and 82% in the gastric cancer group and 85%, 71%, 55%, 43%, 61% and 75% in the control group, respectively. There was no statistically significant difference (p> 0.05) between gastric carcinoma and control groups in terms of CagA, HSP and flagellin antibodies (p> 0.05). On the other hand, a statistically significant difference was found between the 2 groups in terms of urease-A, urease-B and OMP-67 (p< 0.01). These results suggested that this test should be assessed again by the manufacturer for its detection power directed towards specific H.pylori antibodies, especially for Vac-A. Further molecular and clinical studies are necessary to determine the factors that affect H.pylori virulence and disease prognosis.
Vanc Gene-Related Intrinsic Teicoplanin Resistance Detected in Enterococcus Casseliflavus and E-Gallinarum Strains by the Bd Phoenix Automated Microbiology System
(Amer Soc Microbiology, 2008) Berktas, Mustafa; Yaman, Gorkem; Ozturk, Oznur