Browsing by Author "Panhwar, Sallahuddin"

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Dual Responsive Disposable Electrode for the Enumeration Ofescherichia Coliin Whole Blood
(Wiley-v C H verlag Gmbh, 2020) Panhwar, Sallahuddin; Ilhan, Hasan; Hassan, Syeda Sara; Zengin, Adem; Boyaci, Ismail Hakki; Tamer, Ugur
In this report, we have designed a dual responsive disposable electrode for the enumeration ofEscherichia coli K12 (E. coli K12). The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal-organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro-electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantifyE. coliwith a ranging from 10(1)to 10(7) cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.
Rapid Bacterial Detection Through Microfluidic Integration With a Glucometer
(Elsevier Science Sa, 2025) Eryilmaz, Merve; Ilbasmis-Tamer, Sibel; Panhwar, Sallahuddin; Tayyarcan, Emine Kubra; Boyaci, Ismail Hakki; Suludere, Zekiye; Tamer, Ugur
We present a novel approach for sensitive and portable detection of pathogenic bacteria, which is crucial for household and clinical practice. Our method employs immunoliposomes, antibodies, and microchip to detect specific pathogens quantitatively. Gold and metal metal-organic nanoparticles and liposomes were characterized using high-resolution techniques like TEM and SEM. Utilizing a commercial, personal glucose meter (PGM), we initially detected released glucose from antibody-modified liposomes and microchips with MOF-NPs. Detection on the microchip was achieved within 30 min, while the PGM analysis took only one minute for targeted bacteria, yielding glucose signals of 66 mg/dL and 69 mg/dL, respectively. Serial dilutions with group A-Streptococcus pyogenes (GAS) (1.4 x 10<<^>>4-1.4 x 10<<^>>8 CFU/mL) demonstrated quantitative measurement applicability. This innovative approach and a portable PGM hold promise for various industries, including physician labs, hospitals, and households.