Browsing by Author "Ragbetli, C."

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Acute Myotoxic Effects of by Infusion of Prilocaine and Lidocaine in Rats
(Medwell online, 2009) Ragbetli, M. C.; Yalama, M.; Erdogan, E.; Cengiz, N.; Kati, I.; Ragbetli, C.
We assumed to examine the acute myotoxic effects of infusion of the local anesthetic lidocaine and prilocaine on the gluteus maximus muscle after continuous peripheral nerve blockade in rats. Eight adult female Sprague-Dawley average weighing 150-200 g rats were used in this study. Firstly, all of the animals were anesthetized with ketamine (50 mg kg(-1)) for 6 h. Then, prilocaine (right) and lidocaine (left) were used in equal volume (5 mg mL(-1)) for continuous peripheral nerve blockades of the posterior extremity at a rate of 0.3 mL h(-1) for a total period of 6 h in 5 animals. The remaining 3 animals as control group were treated with physiological saline on both sides at 0.3 mL h(-1) for a total period of 6 h. For routine histological observation, the infusion area was dissected and tissue samples including peripheral muscle were taken. For routine histological observation, the infusion area was dissected and tissue samples including peripheral muscle were taken. Method histological hazards as myotoxicite were not observed in skeletal muscle tissue after the infusion of prilocaine and lidocaine. There were no complications cases of local anesthesia. Prilocaine and lidocaine as local anesthetics might be applied safely by infusion.
Antioxidant Vitamins and Microminerals in Cows With Foot-And Disease
(Agricultural Research Communication Centre, 2014) Yoruk, I. H.; Tanritanir, P.; Dede, S.; Ceylan, E.; Ragbetli, C.
Foot and-mouth disease (FMD) is an acute, highly contagious inflammatory viral disease of domestic and wild animals which causes decrease in productivity and lead to mortality. This study was conducted to investigate the levels of copper, zinc, magnesium and iron, alpha-tocopherol, vitamin D-3, vitamin C and lipid peroxidation in cow with foot-and-mouth disease. Sixty native black cows infected by foot-and-mouth disease and twenty healthy controls were selected for the study after clinical and serological examination. In the diseased animals, the malondialdehyde (p<0.00) and-copper (p<0.001) levels were increased while the vitamin C, alpha-tocopherol (p<0.001), vitamin D3 (p<0.05) magnesium (p<0.001) and iron (p<0.005) levels were decreased relative to controls. Although the zinc level was not changed, the Cu/Zn ratio was elevated in the diseased group. The obsereved changes may be the result of secondary infections due to suppressed immune system and losses of tissue and mucosa associated with foot-and-mouth disease.
Effect of Streptozotocin on Biochemical Parameters in Rats
(Chemical Publishing Co., 2010) Ragbetli, C.; Ceylan, E.
This study was carried out to investigate whether streptozotocin induced diabetic rats could effect some of serum biochemical parameters. Twenty Sprague-Dawley albino rats were divided into two experimental: Control (50 mg/kg SF) and streptozocin (60 mg/kg), Control rats were given only physiological saline (50 mg/kg). Serum glucose, AST, ALT and cholesterol levels of rats were determined after treatment of 1, 3 and 6 h, while the level of glucose, aspartate amino transferase (AST) and alanine amino transferase (ALT) were increased and cholesterol level was decreased. It is concluded from the experimental study that streptozocin (60 mg/kg) may increase of serum glucose, AST, ALT levels and cause diabet in rats.
The Effect of Tulathromycin Treatment on Antioxidant Vitamins in Montofon Calves With Pneumonia
(Medwell online, 2009) Ragbetli, C.; Tanritanir, P.; Yoruk, I.; Cay, M.
The purpose of this study is to examine changes in some vitamin levels and clinical symptoms of treatment with tulathromycin in Montofon calves with pneumonia. For this purpose, 30 calves exhibiting clinical sings of pneumonia such as fever >39 degrees C, elevated respiratory rate >40 (min(-1)), pulsation >100, cough, lack of appetite, nasal flaring, mandibular lymph node enlargement, respiratory difficulties and nasal discharge were studied. A control group of 20 healthy calves were also studied. Blood samples were collected from the diseased calves before and after treatment with tulathromycin (Draxxin-Pfizer), as well as from the healthy calves in order to determine retinol (mu g mL(-1)), alpha-tocopherol (mu g dL(-1)) and vitamin D-3 (mu g dL(-1)) levels by High Performance Liquid Chromatography (HPLC). Compared to the control group, there were increase of both retinol and alpha-tocopherol levels in the diseased animals and the increase in retinol levels were significant (p<0.001). Vitamin D-3 levels weren't affected in the diseased animals (p>0.05). Abnormal rectal temperatures, respiratory rates and pulsation levels decreased significantly (p>0.001).
The Effect of Tulathromycin Treatment on Biochemical Parameters in Montofon Calves With Pneumonia
(Academic Journals inc, 2010) Ragbetli, C.; Ceylan, E.; Tanritanir, P.
The purpose of this study is to examine changes in some biochemical parameters of treatment with tulathromycin in Montofon calves with pneumonia. For this purpose, 30 claves exhibiting clinical sings of pneumonia with fever, cough, lack of appetite, nasal flaring, mandibular lymph node enlargement dyspnea and nasal discharge were studied. A control group of 20 healthy calves was also studied. Blood samples were collected from the healthy and diseased calves before and after treatment with Draxxin (Tluthromycin-Pfizer). Compared to the control group Ca, K, total bilirubin, albumin, urea levels were affected (p<0.001) and Na (p<0.05) in the diseased animals and total protein, ALP, Glucose, Creatin levels were not affected in the diseased animals (p>0.05). Single dose tulathromycin therapy was characterized by significantly fewer treatment days, a higher response rate, significantly better reduction of pyrexia and fever and other pneumonia symptoms in calves requiring treatment.
Effect on Biochemical Parameters in Naturally Infected Calves With Giardiasis After Treatment With Albendazole in Van Province, Turkey
(2014) Ragbetli, C.; Tanritanir, P.; Yilmaz, H.; Yoruk, I.; Ozdemir, H.
The aim of this study was to investigate the changes in the blood serum levels of some biochemical parameters, lipid-soluble vitamins and trace elements in the calves naturally infected with Giardia spp. in Van region, before and after treatment with orally 20 mg/kg-1 albendazole three successive days. Serum samples were analyzed for biochemical parameters and some vitamins. In the infected animals, concentrations of creatinine, amylase levels were lower (p< 0.05). Glucose, urea, total protein, AST, ALT, sodium, potassium, retinol and α-tocopherol levels were seen significantly higher in the after treatment group (p< 0.05). There was no statistically significant difference in the concentration of total bilirubin, chloride and vitamin D3 between groups. All the animals were not encountered cysts or trophozoites of Giardia at the end of the treatment. The results of this study would suggest albendazole to be 100% effective against giardiasis in all of the calves in Van region.
The Efficacy of Moxidectin Against Gastrointestinal Nematode Infections in Goats
(2009) Ragbetli, C.; Ceylan, E.; Tanritanir, P.
The aim of this study was to examine the efficacy of rnoxidectin treatment on goats naturally infected with gastrointestinal nematodes in Van region, Turkey. Two hundred and forty goats infected with gasterointestinal parasites were treated with 0.2 rug kg-1 moxidectin (Cydectin, Abfar), subcutaneously. Ten randomly selected goats were not treated and allocated as a control group. Faecal samples were examined for gastrointestinal parasites qualitatively and quantitatively (EPG) in 0th, 7th and 14th days of treatment Larvae of the parasite species of Ostertagia, Haemonchus, Nematodirus and Trichostrongylus were detected in the coprocultures of the infected animals performed before treatment. It was observed that moxidectin was 100% effective against the gastrointestinal nematodes. © 2009 Academic Journals Inc.
Evaluating the Effect of the Treatment of Doramectin on Some Biochemical Parameters in Goats Naturally Infected With Gastrointestinal Nematodes
(Academic Journals inc, 2010) Ceylan, E.; Ragbetli, C.; Tanritanir, P.
The aim of this study was to investigate the therapeutic effect of doramectin applied once in an optimum dose on stomach-intestine nematodes on goats naturally infected with gastrointestinal nematodes. Alto to study the effect of the doramectin application on the clinically important biochemical parameters, electrolytes, minerals, vitamin B-12 levels and enzyme activities of goats were affected or not after anthelmentic drug application. For this purpose, the stools in 45 goals between 1-3 year were chosen in Van province and subjected to Fulleborn saturated salt solution method. Thirty goats with gastrointestinal nematodes constituted treatment group and they were doramectin applied (group I). Other 15 goats with gastrointestinal nematodes chose as control group (group II) was not Doramectine applied. Taking stool samples goats with Doramectin treatment and from control group on the 0, 7 and 14 days of application in gram stools, the number of eggs (EPG) were analyzed by modified McMaster method and treatment efficiency was a investigated. After coprocultures, goats were determined to be infected with Trichostrongylus sp., Nematodirus sp., Ostertagia sp., Marshallagia sp., Haemonchus sp. and Oesphagostomum sp. The results demonstrated that Fe, Albumin and hemoglobin levels statistically increased in treatment group in comparison with control group (p<0.05); a significant change in IBP, Ferritin, ALT, AST, total protein, P, Ca, Na and K levels was not observed and minor increases along with the treatment related to time in vitamine B-12, ALP and Mg levels occurred. It was concluded that in goats, one dose of 0.2 mg kg(-1) of subcutaneous doramectin was efficient 100% and no side-effect was observed.
Histopathological Effects of Desflurane on the Liver and the Kidney Using Light Microscopy
(2010) Kati, I.; Tekin, M.; Tas, A.; Ragbetli, C.; Ugras, S.
The aim of this study was to histopathologically assess the toxic effect of desflurane on the liver and the kidney. The rats (n = 150) were grouped into five main and three subgroups according to the exposure time to the anesthetic agent. The first group was planned as 1 MAC Desflurane+N2O+O2, the second group as 1 MAC Desflurane+O2, the third group as 2 MAC Desfiurane+N2O+O2, the fourth group as 2 MAC Desflurane+O2 and the 5th group as the control group. Each group was given the anesthetic agent for 1, 3 or 6 h. The rats that were exposed to the anesthetic agent with an adequate dose and for an adequate period of time were sacrificed properly. Livers and kidneys were resected and examined by light microscopes following the routine tissue follow-up procedures. Significant histopathological changes were observed in all groups compared to the control group. However, the pathological findings of the group 2 MAC Desflurane+N2O+O2 at the 6th h with the light microscope was significantly more than those of other groups at all times. It was concluded that although, desflurane caused cellular changes in liver and kidney tissue, it did not cause any clinical changes. Nevertheless, further studies are required to support this opinion. © Medwell Journals, 2010.
The Investigations of Total Antioxidant Status and Biochemical Serum Profile in Thymoquinone-Treated Rats
(African Networks Ethnomedicines, 2015) Kurt, E.; Dede, S.; Ragbetli, C.
Background: This study was planned to determine the dosage and duration of thymoquinone (TQ) at which it demonstrates the optimum effect on the total antioxidant status (TAS) and the biochemical parameters in the blood serum. Materials and Methods: 48 male rats (Wistar-albino) weighing between 200-250 g were used as material. Group 1 (control) (TQ solution 5 mg/kg/day), Group 2 (15 mg/kg/day), Group 3 (30 mg/kg/day), Group 4 (45 mg/kg/day) and Group 5 (60 mg/kg/day) were designated, each containing 8 rats. Different doses of TQ (oral gavage) were administered for four weeks. Results: The TAS levels were determined to be considerably low statistically in all TQ-administered groups in comparison with the control group. It was determined that the serum biochemical parameters exerted a significant effect depending on the TQ doses. Conclusion: As a result, rats administered with TQ orally, at 60 mg/kg dosage, show that the liver and kidney function parameters in particular as different from normal. This brings us to the conclusion that at this dosage, there is reliable biochemical wise but future protective studies in which 30 mg/kg doses can be used safely is encouraged.
Long-Term Effects of Deslorelin on Ovarian Preantral Follicle Number in Female Rats: Preliminary Stereological Observations
(Wiley-blackwell, 2011) Alkis, I.; Sendag, S.; Cetin, Y.; Akyol, V.; Ragbetli, C.; Wehrend, A.; Ragbetli, M.
The Serum Protein Fractions in Streptozotocin (Stz) Administrated Rat Models
(EManuscript Services, 2017) Ragbetli, C.; Dede, S.; Koc, F.; Yuksek, V.; Ragbetli, M.C.
Background: Experimental diabetes can be induced using chemical agents such as streptozotocin Objective: This study aimed to investigate the effect of streptozotocin (STZ) which is most important agent to produce experimental diabetic model at two different doses on serum protein fractions in rat models. Materials and Methods: Twenty-four male rats that weighed an average of 250 g and were 3-4 months old were used as the experimental models. They were sorted into three groups composed of eight rats each of STZ 55 mg/kg, STZ 65 mg/kg and control. Diabetes was induced by administering STZ 55 mg/kg and 65 mg/kg intraperitoneally. The serum protein fractions were analyzed by cellulose acetate electrophoresis. Results: No significant difference was observed between the groups for all fractions except alpha-2 and beta globulins. The alpha-2 and beta globulin levels were significantly higher in the 55 mg/kg group than in the 65 mg/kg STZ and control groups (p<0.05). Conclusion: This increase may be due to the involvement of different proteins in the alpha-2 and beta globulin protein fractions. © 2016 Phcog.Net.