Browsing by Author "Ragbetli, M. C."

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Acute Myotoxic Effects of by Infusion of Prilocaine and Lidocaine in Rats
(Medwell online, 2009) Ragbetli, M. C.; Yalama, M.; Erdogan, E.; Cengiz, N.; Kati, I.; Ragbetli, C.
We assumed to examine the acute myotoxic effects of infusion of the local anesthetic lidocaine and prilocaine on the gluteus maximus muscle after continuous peripheral nerve blockade in rats. Eight adult female Sprague-Dawley average weighing 150-200 g rats were used in this study. Firstly, all of the animals were anesthetized with ketamine (50 mg kg(-1)) for 6 h. Then, prilocaine (right) and lidocaine (left) were used in equal volume (5 mg mL(-1)) for continuous peripheral nerve blockades of the posterior extremity at a rate of 0.3 mL h(-1) for a total period of 6 h in 5 animals. The remaining 3 animals as control group were treated with physiological saline on both sides at 0.3 mL h(-1) for a total period of 6 h. For routine histological observation, the infusion area was dissected and tissue samples including peripheral muscle were taken. For routine histological observation, the infusion area was dissected and tissue samples including peripheral muscle were taken. Method histological hazards as myotoxicite were not observed in skeletal muscle tissue after the infusion of prilocaine and lidocaine. There were no complications cases of local anesthesia. Prilocaine and lidocaine as local anesthetics might be applied safely by infusion.
A Morphological and Stereological Study on Cervical Segment of Spinal Cord of Quails
(Wiley, 2017) Cakmak, G.; Soyguder, Z.; Ragbetli, M. C.
In this study, volume densities of white and grey matters of cervical segments of spinal cords of quail were investigated stereologically. In both sexes, mature, six quails were used as material of this study. All animals were fixed by perfusing in 10% buffered formalin. Tissue specimens were obtained from cervical spinal cords. These tissue specimens were cut every fiftieth section at 5m thickness by a microtome. And mean six or seven sections were examined from every block by this method at microscope. After that, these sections were stained by haematoxylin eosin and photographed. Densities of volumes of all tissues of cervical segments of whole spinal cords and white and grey matters were calculated with principle of Cavalieri. As a result, total volume of spinal cord, volumes of white and grey matters of cervical segment and volume rates of white and grey matters were calculated.
Silibinin and Ellagic Acid Increase the Expression of Insulin Receptor Substrate 1 Protein in Ultraviolet Irradiated Rat Skin
(Taylor & Francis Ltd, 2020) Gorgisen, G.; Ozkol, H.; Tuluce, Y.; Arslan, A.; Ecer, Y.; Keskin, S.; Ragbetli, M. C.
Daily exposure to ultraviolet (UV) light induces inflammation and tumorigenesis in the skin. Silibinin and ellagic acid are natural products that exhibit anti-inflammatory and anti-tumorigenic properties. Insulin receptor substrate protein 1 (IRS1) is important for skin homeostasis and physiology, but its activity following UV radiation remains unclear. We investigated the effects of ellagic acid and silibinin on IRS1 expression in ultraviolet A (UVA) and ultraviolet B (UVB) irradiated rat skin. Forty-two female Wistar rats were divided randomly into six groups of seven animals. The dorsal skin of rats was exposed to UVA + UVB, then treated with ellagic acid and silibinin by gavage. IRS1 expression in skin tissues was determined by western blot analysis. IRS1 expression increased significantly following treatment with ellagic acid and silibinin in UVA + UVB irradiated skin compared to the UVA + UVB only group. After UVA + UVB treatment, ellagic acid effected greater induction of IRS1 expression than silibinin. Our findings suggest that the photoprotective roles of ellagic acid and silibinin may be due to induction of IRS1 expression in UVA + UVB treated rat skin.
Stereological and Histopathological Evaluation of Ovary and Uterine Horns of Female Rats Prenatally Exposed To Diclofenac Sodium
(informa Healthcare, 2013) Guven, D.; Altunkaynak, B. Z.; Ayranci, E.; Kaplan, S.; Bildircin, F. D.; Kesim, Y.; Ragbetli, M. C.
In this study, we investigated the morphometric and histological alterations of the ovary and uterine horns in 4-week-old rats that were prenatally exposed to diclofenac sodium (DS). For this purpose, pregnant rats were divided into two groups: the control and drug-treated groups. Beginning from the 5th day after mating through the 15th day of pregnancy, DS (1 mg/kg daily) was intraperitoneally injected in the treated group. No injection was given to the rats in the control group. After spontaneous delivery, male off spring were obtained. At the end of the 4th week, ovary and uterine horn samples were removed. Following dissection and routine histological preparation, histopathological and stereological investigations were carried out. Our results indicate that DS application leads to a decrease in the mean volume fraction of the uterine horn. Moreover, there was an increased volume fraction in some structures of the ovary; like the cortex, medulla and zona granulosa. There was no difference found between the two groups in terms of the mean volume of the antrum and the Graafian follicle fraction. Finally, in light of our findings, we may suggest that DS may lead to adverse effects in rats that are prenatally subjected to this drug.