Browsing by Author "Söğütlü, İnci"

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The Effects of Grape Seed Extract on Insulin, Adiponectin and Resistin Levels in Diabetic Rats
(2021) Yılmaz, Halil Cumhur; Mert, Nihat; Mis, Leyla; İrak, Kıvanç; Mert, Handan; Söğütlü, İnci
In this study, the effects of grape seed extract on insulin, adiponectin and resistin levels in diabetic rats were aimed to investigate. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats were used. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats randomly divided into four groups of eight each: control group (C), group with diabetes (DM), grape seed extract group (GSE), diabetes mellitus and grape seed extract group (DM+GSE). The diabetic group 45 mg/kg single dose of streptozotocin was administered intraperitoneally, and the grape seed extract groups (DM+GSE and GSE) grape seed extract was given orally every day with an intragastric tube for 20 days (0.6 ml/rat). Blood samples were taken from the hearts of rat’s end of the experiment. The sera obtained were used for insulin, adiponectin and resistin analysis measured via ELISA with commercial kits. The insulin and adiponectin levels of the rats in the diabetes + grape seed extract group were found to be higher than those in the diabetes group. There was no significant change in the resistin level differences between the groups. As a result; It is possible to say that grape seed extract has an increasing effect on insulin level and adiponectin levels of diabetic rats are decreased due to diabetes and it has no significant effect on resistin levels. Detected by the positive effects of grape seed extracts, it may be useful to use for the regulating of the diabetes in human.
Honamli Goats Breed in South of Turkey I- Serum Mineral Analysis
(2020) Ayşin, Nesrullah; Mert, Handan; Mert, Nihat; Demir, Ayse Ozge; Söğütlü, İnci; İrak, Kıvanç
The objectivity of this study was to investigate some blood minerals and parameters in Honamli goats reared under semi-intensive conditions. 90 goats (2-4 ages) in different three herds kept under similar manage mental conditions were chosen as research materials in Teke Border of Antalya Province. Serum Ca 6.786±0.206 mg/dL, P 4.094±0.173 mg/dL, Cl 111.105±0.582 mmol/L, K 4.519±0.066 mmol/L, Mg 2.161±0.05 mg/dL, Na 148.047±0.508 mmol/L, Fe 110.706±2.510 μg/dL, UIBC 155.025±4.333 μg/dL and Tp 7.055±0.120 g/dL were determined by Roche Diagnostics, Cobas 8000 modular analyser series, immunoassay. After then, total iron- binding capacity (TIBC), transferrin (Tf), transferrin saturation (TSAT) and Ca++ levels were calculated from each other using different biochemical formulas. Ratios between the some minerals were calculated and presented in the text. Also, very high significant differences (P<0.0001) were found between herds for P, Cl, TIBC and Tf. However, no significant differences were found for Ca, Na, TSAT, TP and Ca results. Coefficients of variation (CV) were within 2 % and 35 % for all outcome parameters. In Pearson correlation analysis, negative and different two significant values (P<0.01 and P<0.001) were calculated between Fe and unsaturated iron binding capacity (UIBC) for all analysed groups. Controversially, positive and very high significant correlations (P<0.0001) were detected between Cl and Na. The results obtained from this study could serve as reference values for Honamli goats breeding in Mediterranean Region of Turkey.
Some Macromineral and Trace Mineral Levels in Milk of Different Dog Breeds
(2022) Mert, Handan; Mert, Nihat; Söğütlü, İnci; Cellat, Mustafa; Günbatar, Nizamettin
Objective: In this study, it was planned to examine the mineral levels in the milk of different breed dogs during the lactation period. Calcium, magnesium, potassium, sodium, copper, zinc, manganese and iron concentrations were analyzed in dog milk throughout the lactation period.\rMaterials and methods: In this research 6 Labradors, 6 German Shepherds, 6 Pointers, 5 Turkish Tazis, 5 Setters, 7 Malinois, and 5 Golden Retrievers (a total 40 dogs) of 3 to 4 years age were used as research materials, all of which were under same management and feeding conditions. All dogs were on diets appropriate for gestation and lactation periods. Adequate milk volume could be collected 2-3 weeks after parturition, and there were no known medical problems. Each day's samples were kept capped and refrigerated after being collected. The concentrations of calcium, magnesium, potassium, sodium, copper, zinc, manganese and iron, were analyzed by using Varian Brand 30/40 model AAS device. \rResults: The Ca, K, Na, Zn, Mn and Fe levels of milk samples from different dog breeds were found to have no significant difference . The highest Mg level was determined in Pointer breed milk samples, and the lowest was determined in Setter milk samples. The highest Cu levels amongst the inspected races were in Labrador milk samples, whereas the lowest levels were determined in Setter breeds. \rConclusion:This data shows that most of the analyzed milk content of different breeds of dogs did not change significantly during the same lactation period, and any present difference could be taken into account when evaluating breeding studies.