Browsing by Author "Sagbas, S."
Now showing 1 - 2 of 2
- Results Per Page
- Sort Options
Article Degradable Natural Phenolic Based Particles With Micro-And Nano-Size Range(Bentham Science Publishers B.V., 2018) Sahiner, N.; Sagbas, S.; Sahiner, M.; Aktas, N.Background: Degradable polymeric particles derived from phenolic compounds are promising materials for biomedical applications due to their inherently antioxidant, antimicrobial, and anticancerogenic properties. We revise all the patent regarding to the biomedical and food additive formulations of Rutin (RT) and Quercetin (QC) as phenolic compounds. Objective: Prepare degradable Poly(Rutin) (p(RT)) and Poly(Quercetin) (p(QC)) particles from natural phenolic compounds, Rutin (RT) and Quercetin (QC). Method: P(RT), and p(QC) particles were prepared using microemulsion crosslinking method employing phenolic compounds such as RT and QC as monomer and poly(ethylene glycol) diglycidyl ether (PEGGE) as a crosslinker in a single step. The degradability of these particles was investigated at physiological conditions, pH 5.4, 7.4, and 9 at 37.5°C. The antioxidant capacity of RT, QC and their corresponding particles was determined by means of total phenol content and ABTS + scavenging assay. The blood compatibility of the particles is determined with hemolysis and blood clotting tests, and the cytotoxicity of the particles on L929 fibroblast cell and A549 cancer cells was done by WST-1 tests. Results: The size of the prepared phenolic particles was in the size range of 0.4-4 μm with negative zeta potentials,-20.29±1.7 and-31.31±2.0 mV for p(RT) and p(QC) particles, respectively. The highest amount of degradation was obtained for p(QC) particles in almost a linear profile with relatively longer time degrading kinetics at pH 9, e.g., 197±23 mg/g QC was released up to 130 h. The antioxidant capacities of phenolic compounds were decreased about ten-fold upon the particle formations of the phenolic compound, and the antioxidant capacity of p(QC) particles was found to be better than p(RT) particles with 0.22±0.01 and 0.05±0.001 μmol trolox equivalent g -1 , respectively. The blood compatibility test of p(RT) and p(QC) particles revealed that both particles are blood compatible up to 1 mg/mL concentration and possess clotting of blood over 1 mg/mL concentrations. Furthermore, the cytotoxicity tests showed that p(RT) particles are more biocompatible than p(QC) on the fibroblast cell as 91% cell viability versus 50% for p(QC) was observed at 75 μg/mL particle concentrations. Additionally, at this concentration 42.3% of cancer cells were inhibited by p(RT) particles. Conclusion: Degradable p(RT) and p(QC) particles that are prepared in a single step offer great avenue for biomedical applications as highly antioxidant materials and with good biocompatibility in contact with blood and fibroblast cells, as well as great anticancerogenic capability against the cancer cells. © 2018 Bentham Science Publishers.Conference Object The Utilization Smart Hydrogels and Composites With Controllable Porosity in the Preparation of Metal Nanocatalyst(Crc Press-taylor & Francis Group, 2012) Sahiner, N.; Ozay, O.; Sagbas, S.; Yasar, A.; Aktas, N.The hydrogels from 2-acrylamido-2-methyl-1-propansulfonic acid (AMPS), 2-acrylamido glyconic acid (AAGA), N-vinyl imidazole (VI), and sulfopropylmethacrylate (SPM) that can absorb metal ions such as Co, Ni, Rh, Ru ye Pt from aqueous media were used in the preparation of homo or copolymeric hydrogels and their organic-inorganic composites with tetraethoxy silane (TEOS), tertamethoxysilane (TMOS) and vinyltriethoxysilane (VTEOS), or vinyltrimethoxyilane (VTMOS) as hydrogel-silica composites. The prepared hydrogel and their silica composites were used as template in Cu, Ag, Co, Ni, Fe etc, and magnetic ferrite particle preparation. The prepared hydrogel metal composites were used as a smart catalyst system with controllable porosity for the production of hydrogen in the hydrolysis of different hydrites such as NaBH4 is a novel concept and a smart approach.