Browsing by Author "Saglam, Y. S."

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Effect of Pomegranate (Punica Granatum L.) Juice on Kidney, Liver, Heart and Testis Histopathological Changes, and the Tissues Lipid Peroxidation and Antioxidant Status in Lead Acetate-Treated Rats
(C M B Assoc, 2017) Aksu, D. S.; Saglam, Y. S.; Yildirim, S.; Aksu, T.
Pomegranate juice (PJ) contains relevant amounts of active biological compounds which alleviate the detrimental effects of chronic heavy metal exposure. This study investigated the protective potential of PJ against lead-induced oxidative stress. A total of forty adult male Sprague Dawley rats were divided into four experimental groups. The animals were fed a standard pellet diet and tap water ad libitum. The rats were divided into four groups (n=10 for each group): control, lead asetat (2000 ppm), low-treated PJ- a daily dose of 2.000 ppm lead plus 30 mu l pomegranate juice (included 1.050 mu mol total polyphenols, gallic acid equivalent), and high-treated PJ- a daily dose of 2.000 ppm lead plus 60 mu l pomegranate juice (included 2.100 mu mol total polyphenols, gallic acid equivalent). The treatments were delivered for 5 weeks. After the treatment period, the tissues samples (kidney, liver, heart and testis) were collected. Tissue lead (Pb) and mineral amounts (copper, zinc, and iron), tissues lipid peroxidation level and antioxidant status, and tissues histopathological changes were determined. The results showed that the highest rate lead loading was in the kidney and the testis. Pomegranate juice was decreased the lead levels of soft tissues examined; increased Zn amounts in tissues of which the lead accumulation was higher (kidney and the testis); decreased the copper, zinc and the iron levels of the liver and heart tissues, without creating a weakness in antioxidant capacity of these tissues, restricted the oxidative stress by decreasing lipid peroxidation, improved both of the activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalaz (CAT), and the level of glutathione (GSH) in all the tissues examined in lead-treated groups. As histopathological findings, the cellular damage induced by lead in the tissues of the kidney, liver and the heart were observed to have been partially prevented by PJ treatment. The protective effect of PJ was more pronounced in the testis compared to those others.
Immunohistochemical Detection of Leptospiral Antigens in Cases of Naturally Occurring Abortions in Sheep
(Elsevier Science Bv, 2008) Saglam, Y. S.; Yener, Z.; Temur, A.; Yalcin, E.
This study was carried out to determine the leptospiral antigens in naturally occurring 108 abort sheep fetuses. To determine the antigen localizations in tissue sections (kidney, liver, lung and spleen) of each fetus were stained with immunoperoxidase (IP) technique and then were examined under light microscope. The results of this study showed that 19 (17%) out of 108 fetuses were positive for the presence of leptospiral antigens. In the 19 positive cases, leptospiral antigens were found in lung (n = 10; 9%), liver (n = 7; 6%), kidney (n = 12; 11%) and spleen (n = 2; 2%) samples. Microscopic studies demonstrated that leptospiral antigens were located in the cytoplasm of macrophages in interalveolar and interlobular septum of the lung; in the cytoplasm of macrophages in the portal regions and hepatocytes of the liver; in the cytoplasm of epithelial cells of renal pelvis, in the cytoplasm of epithelial cells of cortical and medullar tubules, and macrophages of intertubular region in the kidney. In the spleen, antigens were detected in the cytoplasm of macrophages throughout the parenchymal tissue. In conclusion, the results of this study demonstrate that leptospirosis could be a major disease causing abortions in sheep. (C) 2007 Elsevier B.V. All rights reserved.
Immunohistochemical Detection of Mannheimia (Pasteurella) Haemolytica Antigens in Goats With Natural Pneumonia
(Springer, 2009) Yener, Z.; Ilhan, F.; Ilhan, Z.; Saglam, Y. S.
Pneumonia is a leading cause of loss to ruminants throughout the world. Mannheimia (Pasteurella) haemolytica is one of the most important etiological agent of pneumonia in cattle, sheep, and goats. This study was carried out to determine the incidence of M.haemolytica antigens using immunohistochemistry labelling of formalin-fixed, paraffin-embedded tissues in pneumonic lungs of goats slaughtered at abattoir, and then to compare these immunohistochemistry results with the results of bacterial isolation. For these objectives, a total of 1505 goat lungs slaughtered in slaughterhouse were grossly examined and pneumonia was detected in 74 cases (4.91%). Of these, with the exception of verminous pneumonia observed in 32 cases, on 42 pneumonic lungs immunohistochemical examinations were performed. Formalin-fixed and paraffin-embedded lung tissue samples were immunohistochemically stained by the avidin-biotin-peroxidase complex (ABC) procedure using polyclonal antibodies to detect M.haemolytica antigens. Pneumonic lesions were more frequently encountered in cranioventral lobes than caudal lobes, and characterized by irregular lobular foci of atelectasis or lobar pneumonia. The presence of M.haemolytica antigens was detected in 19 (45%) out of 42 pneumonic lungs. Bacterial antigens were found most frequently in the cytoplasm of bronchial and bronchiolar epithelial cells, in the swirling degenerating leukocytes in the alveoli, and in the degenerating leukocytes in the area of coagulation necrosis, less frequently in the epithelial cells of bronchial glands, and lymphoid cells. Conclusionly, immunohistochemical detection of M.haemolytica antigens in pneumonic lungs appear to be more reliable compared to bacterial isolation.